Journal ArticleWound Repair Regen · May 2022
Pyoderma gangrenosum (PG) has been linked to various underlying systemic diseases; many associations are based on case reports or small case series, including hidradenitis suppurativa. Literature examining systemic therapies according to underlying comorbi ...
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Journal ArticleCurrent Dermatology Reports · December 1, 2016
Purpose of Review: This review provides a thorough qualitative synthesis of the characteristic physical examination findings, expected diagnostic study findings, risk factors, etiology, systemic complications, and optimal therapeutic approach to leg ulcers ...
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Journal ArticleWound Repair Regen · September 2016
The present status of clinical leg ulcer healing research was reviewed by 25 experts over 2 days on September 28 and 29, 2015. Multiple clinical effectiveness reviews were presented suggesting that published clinical wound healing research often does not m ...
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Journal ArticleBritish Journal of Dermatology · May 1, 2016
The effect of sex hormones on pyoderma gangrenosum (PG) has not been reported. We report the case of a 34-year-old woman with chronic PG leg ulcers who was found to have recurring, premenstrual flares of PG. Her PG flares were controlled with the use of et ...
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Journal ArticleSemin Vasc Surg · 2015
Venous ulcer of the lower extremity is a common vascular condition and is associated with decreased quality of life, reduced mobility, and social isolation. Treatment of chronic venous ulcer (CVU) includes compression therapy, debridement of the ulcer when ...
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Journal ArticleWound Repair Regen · 2015
The use of negative pressure wound therapy (NPWT) is increasing in both the inpatient and outpatient settings. We conducted a systematic review on the efficacy and safety of NPWT for the treatment of chronic wounds in the home setting. We searched MEDLINE, ...
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Journal ArticlePLoS One · 2015
Chronic, non-healing wounds contribute significantly to the suffering of patients with co-morbidities in the clinical population with mild to severely compromised immune systems. Normal wound healing proceeds through a well-described process. However, in c ...
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Journal ArticleWound Repair Regen · 2014
The purpose of this study was to systematically review the literature on the benefits and harms of advanced wound dressings on wound healing, mortality, quality of life, pain, condition of the wound bed, and adverse events for patients with chronic venous ...
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Journal ArticleJournal of Vascular Surgery: Venous and Lymphatic Disorders · January 1, 2014
Objective: Chronic venous ulcers (CVUs) remain the leading causes for nonhealing wounds in the lower extremities. Although multilayer compression dressing remains the treatment gold standard, there are various surgical procedures aimed at healing CVUs with ...
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Journal ArticleWound Repair Regen · 2014
The prevalence and costs of chronic venous ulcer care in the US are increasing. The Johns Hopkins University Evidence-Based Practice Center recently completed a systematic review of the comparative effectiveness of advanced wound dressings, antibiotics, an ...
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Journal ArticleBr J Dermatol · October 2011
Human skin is colonized by bacteria. The development of new genomic microbiological techniques has revealed that the bacterial ecology of human skin is far more complex than previously imagined and includes many fastidious or noncultivable bacterial specie ...
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Journal ArticleWound Repair Regen · 2011
Controlling for sample site is considered to be an important aspect of chronic wound microbiological investigations; yet, macroscale spatial variation in wound microbiota has not been well characterized. A total of 31 curette samples were collected at the ...
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Journal ArticleJournal of Investigative Dermatology · January 1, 2011
Lower-extremity wounds are a major complication of diabetes. Hemoglobin A1c (HbA1c) reflects glycemia over 2-3 months and is the standard measure used to monitor glycemia in diabetic patients, but results from studies have not shown a consistent associatio ...
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Journal ArticleWound Repair Regen · 2011
Chronic wounds contain complex polymicrobial communities of sessile organisms that have been underappreciated because of limitations of standard culture techniques. The aim of this work was to combine recently developed next-generation investigative techni ...
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Journal ArticleWound Repair Regen · May 2022
Pyoderma gangrenosum (PG) has been linked to various underlying systemic diseases; many associations are based on case reports or small case series, including hidradenitis suppurativa. Literature examining systemic therapies according to underlying comorbi ...
Full textLink to itemCite
Journal ArticleCurrent Dermatology Reports · December 1, 2016
Purpose of Review: This review provides a thorough qualitative synthesis of the characteristic physical examination findings, expected diagnostic study findings, risk factors, etiology, systemic complications, and optimal therapeutic approach to leg ulcers ...
Full textCite
Journal ArticleWound Repair Regen · September 2016
The present status of clinical leg ulcer healing research was reviewed by 25 experts over 2 days on September 28 and 29, 2015. Multiple clinical effectiveness reviews were presented suggesting that published clinical wound healing research often does not m ...
Full textLink to itemCite
Journal ArticleBritish Journal of Dermatology · May 1, 2016
The effect of sex hormones on pyoderma gangrenosum (PG) has not been reported. We report the case of a 34-year-old woman with chronic PG leg ulcers who was found to have recurring, premenstrual flares of PG. Her PG flares were controlled with the use of et ...
Full textCite
Journal ArticleSemin Vasc Surg · 2015
Venous ulcer of the lower extremity is a common vascular condition and is associated with decreased quality of life, reduced mobility, and social isolation. Treatment of chronic venous ulcer (CVU) includes compression therapy, debridement of the ulcer when ...
Full textLink to itemCite
Journal ArticleWound Repair Regen · 2015
The use of negative pressure wound therapy (NPWT) is increasing in both the inpatient and outpatient settings. We conducted a systematic review on the efficacy and safety of NPWT for the treatment of chronic wounds in the home setting. We searched MEDLINE, ...
Full textLink to itemCite
Journal ArticlePLoS One · 2015
Chronic, non-healing wounds contribute significantly to the suffering of patients with co-morbidities in the clinical population with mild to severely compromised immune systems. Normal wound healing proceeds through a well-described process. However, in c ...
Full textLink to itemCite
Journal ArticleWound Repair Regen · 2014
The purpose of this study was to systematically review the literature on the benefits and harms of advanced wound dressings on wound healing, mortality, quality of life, pain, condition of the wound bed, and adverse events for patients with chronic venous ...
Full textLink to itemCite
Journal ArticleJournal of Vascular Surgery: Venous and Lymphatic Disorders · January 1, 2014
Objective: Chronic venous ulcers (CVUs) remain the leading causes for nonhealing wounds in the lower extremities. Although multilayer compression dressing remains the treatment gold standard, there are various surgical procedures aimed at healing CVUs with ...
Full textCite
Journal ArticleWound Repair Regen · 2014
The prevalence and costs of chronic venous ulcer care in the US are increasing. The Johns Hopkins University Evidence-Based Practice Center recently completed a systematic review of the comparative effectiveness of advanced wound dressings, antibiotics, an ...
Full textLink to itemCite
Journal ArticleBr J Dermatol · October 2011
Human skin is colonized by bacteria. The development of new genomic microbiological techniques has revealed that the bacterial ecology of human skin is far more complex than previously imagined and includes many fastidious or noncultivable bacterial specie ...
Full textLink to itemCite
Journal ArticleWound Repair Regen · 2011
Controlling for sample site is considered to be an important aspect of chronic wound microbiological investigations; yet, macroscale spatial variation in wound microbiota has not been well characterized. A total of 31 curette samples were collected at the ...
Full textLink to itemCite
Journal ArticleJournal of Investigative Dermatology · January 1, 2011
Lower-extremity wounds are a major complication of diabetes. Hemoglobin A1c (HbA1c) reflects glycemia over 2-3 months and is the standard measure used to monitor glycemia in diabetic patients, but results from studies have not shown a consistent associatio ...
Full textCite
Journal ArticleWound Repair Regen · 2011
Chronic wounds contain complex polymicrobial communities of sessile organisms that have been underappreciated because of limitations of standard culture techniques. The aim of this work was to combine recently developed next-generation investigative techni ...
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Journal ArticleClin Microbiol Infect · December 2010
Chronic wounds cause substantial morbidity and disability. Infection in chronic wounds is clinically defined by routine culture methods that can take several days to obtain a final result, and may not fully describe the community of organisms or biome with ...
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Journal ArticleMil Med · July 2010
The Combat Wound Initiative (CWI) program is a collaborative, multidisciplinary, and interservice public-private partnership that provides personalized, state-of-the-art, and complex wound care via targeted clinical and translational research. The CWI uses ...
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Journal ArticleJ Am Geriatr Soc · July 2010
China has the world's largest and most rapidly growing older adult population. Recent dramatic socioeconomic changes, including a large number of migrating workers leaving their elderly parents and grandparents behind and the 4:2:1 family structure caused ...
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Journal ArticleJ Invest Dermatol · January 2010
The role of bacteria in the pathogenesis of chronic, nonhealing wounds is unclear. All wounds are colonized with bacteria, but differentiating colonizers from invading organisms is difficult, if not impossible, at the present time. Furthermore, robust new ...
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Journal ArticlePLoS One · July 31, 2009
BACKGROUND: Bacterial colonization is hypothesized to play a pathogenic role in the non-healing state of chronic wounds. We characterized wound bacteria from a cohort of chronic wound patients using a 16S rRNA gene-based pyrosequencing approach and assesse ...
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Journal ArticleJ Am Acad Dermatol · February 2008
UNLABELLED: Chronic wounds are a major healthcare problem costing the United States billions of dollars a year. The American Academy of Dermatology has underscored the significance of wound care in dermatological practice. It is critical for all dermatolog ...
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Journal ArticleInt J Low Extrem Wounds · December 2007
Advances in digital imaging and archiving have made the measurement and documentation of wound areas possible over time. To assess the reproducibility and precision of digital image measurements, we used WoundMatrix Web (http://www.woundmatrix.com/) and re ...
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Journal ArticleJ Am Acad Dermatol · August 2007
Lipedema is characterized by symmetric lower extremity enlargement secondary to the deposition of fat. Lipedema is not rare, but it is commonly misdiagnosed as lymphedema. We describe a 20-year-old woman with massive lower extremity enlargement that did no ...
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Journal ArticleEmergency Medicine Clinics of North America · January 1, 2007
One of the great misconceptions in wound care is that a wound heals best when permitted to form a dry scab. By contrast, moisture has repeatedly been shown to significantly accelerate wound healing. Emergency physicians and other acute care providers are e ...
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Journal ArticleJ Invest Dermatol · July 1999
Clinical manifestations of mastocytosis are mediated, at least in part, by release of the mast cell mediators histamine and prostaglandin D2. It has been previously reported that in addition to prostaglandin D2, mast cells produce other eicosanoids, includ ...
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Journal ArticleAcad Med · October 1998
PURPOSE: To assess the state of managed care knowledge and attitudes and to evaluate the effects of a two-day course on participants' knowledge, attitudes, and behavioral intentions. METHOD: In 1996, the University of California, Davis, Medical School invi ...
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Journal ArticleBr J Dermatol · February 1997
We report on dominantly inherited epidermal acantholysis in three dogs, a sire and two female offspring. The skin lesions were characterized by hairless, hypertrophic plaques. Histopathologically, these lesions showed epidermal hyperplasia with individual ...
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ConferenceJ Dermatol · November 1996
Human mast cells contain large quantities of chymotryptic and tryptic proteinases. In human skin, mast cells contain both chymase and tryptase, whereas, in the mucosa of the gastrointestinal tract, mast cells contain primarily tryptase. By contrast, submuc ...
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Journal ArticleJ Pathol · March 1996
The plasminogen activators, tissue type and urokinase type (tPA and uPA, respectively), have been identified in various malignancies and have been implicated in both local growth and metastatic spread. To characterize plasminogen activator expression more ...
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Journal ArticleJ Am Acad Dermatol · March 1996
BACKGROUND: The biologically active form of vitamin D3, calcitriol, is effective in the treatment of psoriasis but can alter calcium metabolism. Calcipotriene is an analog of calcitriol that has low calcemic activity and aids in clearing psoriasis. OBJECTI ...
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Journal ArticleJ Am Acad Dermatol · February 1996
BACKGROUND: Chronic venous insufficiency may be associated with lipodermatosclerosis or atrophie blanche. Coagulation abnormalities may be related to these cutaneous disorders. OBJECTIVE: Our purpose was to determine whether fibrinolytic abnormalities exis ...
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Journal ArticleDev Biol · August 1995
Plasminogen activator enzymes have been implicated in the regulation of growth, migration, and differentiation which occur continually in normal epidermis and cyclically in the hair follicle. To elucidate further the importance of plasminogen activation in ...
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Journal ArticleJ Invest Dermatol · June 1995
Symptoms of mastocytosis have been attributed to the overproduction of both histamine and prostaglandin (PG) D2. Recently, we developed an assay for the major urinary metabolite of PGD2 (PGD-M), 9 alpha,11 beta-dihydroxy-15-oxo-2,3,18,19-tetranorprost-5-en ...
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Journal ArticleJ Cell Physiol · May 1995
Autocrine activation of the epidermal growth factor (EGF) receptor on keratinocytes has been recognized as an important growth regulatory mechanism involved in epithelial homeostasis, and, possibly, hyperproliferative diseases. Insulin-like growth factor ( ...
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Journal ArticleArch Dermatol · December 1994
Managed care is the predominant method of health care delivery in Sacramento, Calif; the effect on our academic medical center is profound. The lessons we are learning in the development of a university-based health care network have applicability to derma ...
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Journal ArticleJ Immunol · April 15, 1994
This study establishes the primary structure of human skin chymase and provides further evidence for the presence of a cathepsin G-like proteinase within human mast cells. The amino acid sequence of human skin chymase was established by protein methods and ...
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Journal ArticleArch Dermatol · April 1994
BACKGROUND: Chronic wounds represent a worldwide problem. For laboratory and clinical research to adequately address this problem, a common language needs to exist. OBSERVATION: This language should include a system of wound classification, a lexicon of wo ...
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Journal ArticleExp Dermatol · April 1994
Keratinocytes propagated in low calcium (30 microM CaCl2) serum-free media grow in a monolayer and exhibit morphologic and biosynthetic phenotypes most similar to those of keratinocytes in the basal layer of the normal epidermis. When the calcium in the me ...
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Journal ArticleJ Invest Dermatol · March 1994
The plasminogen activators, tissue type and urokinase type (tPA and uPA, respectively) have been identified in human skin under normal conditions and in various inflammatory dermatoses, including psoriasis. By Northern blot analyses, mRNA for uPA, but not ...
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Journal ArticleHistochemistry · February 1994
The plasminogen activator (PA) proteolytic cascade has been implicated in the regulation of cell activities, including proliferation and differentiation, both of which occur continuously in normal human epidermis and are aberrant in psoriatic epidermis. To ...
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Journal ArticleWound Repair and Regeneration · January 1, 1994
Chronic wounds represent a worldwide problem. For laboratory and clinical research to adequately address this problem, a common language needs to exist. This language should include a system of wound classification, a lexicon of wound descriptors, and a de ...
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Journal ArticleJ Clin Invest · November 1993
Pemphigus vulgaris is an autoimmune blistering disease that is induced by binding of antibodies to a 130/85-kD protein complex on epidermal keratinocytes. An in vivo experimental model of this disease was developed by reconstituting severe combined immunod ...
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Journal ArticleBr J Dermatol · September 1993
Patients with venous leg ulcers have a readily recognized clinical syndrome of shallow ulcers, oedema, leg pain, venous ankle blush, lipodermatosclerosis, varicose veins, hyperpigmentation, and atrophie blanche, and they are assumed to have venous abnormal ...
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Journal ArticleJ Cell Physiol · September 1993
The involvement of protein kinase C (PKC) in epidermal growth factor (EGF)-induced human keratinocyte migration was studied with the phagokinetic assay. It was concluded that PKC activation does not mediate, but rather inhibits, EGF-induced keratinocyte mi ...
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Journal ArticleJ Cell Sci · September 1993
Using immunohistochemistry and in situ hybridization, we have characterized the expression and localization of components of the plasminogen activator proteolytic cascade in an organotypic coculture system which consists of a "dermal" portion (human dermal ...
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Journal ArticleExp Cell Res · September 1993
The sensitivity to serine proteinases of cellular proteins involved in cell-matrix adhesion was investigated using C32 melanoma cells. Cells dissociated from monolayers by the metal chelator ethylenediaminetetraacetic acid were incubated with proteolytic e ...
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Journal ArticleJ Am Acad Dermatol · March 1993
BACKGROUND: Venous leg ulcers are a common cause of morbidity, but few predictive parameters exist that can be used to follow their progress. OBJECTIVE: We investigated the use of healing rate as a useful parameter in the treatment of venous ulceration. ME ...
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Journal ArticleArch Dermatol · August 1992
BACKGROUND AND DESIGN: Pyoderma gangrenosum is a chronic inflammatory ulcerative skin disease of unknown origin, often associated with various diseases including inflammatory bowel disease, inflammatory arthritis, monoclonal gammopathies, hepatitis, and my ...
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Journal ArticleJ Invest Dermatol · March 1992
The presence of plasminogen activators (PA) in a variety of solid tumors appears to correlate, in a number of instances, with enhanced invasive or metastatic capabilities. In the present study, we have immunocytochemically examined basal cell (BCC) and squ ...
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Journal ArticleJ Am Acad Dermatol · February 1992
BACKGROUND: Cyclosporine has been used to treat a variety of cutaneous and immune-mediated diseases. Nephrotoxicity is the most common major adverse side effect. OBJECTIVE: The study was designed to investigate the frequency of alterations in serum creatin ...
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Journal ArticleJ Exp Med · October 1, 1991
Secretory granules of human dermal mast cells contain a chymotrypsin-like serine proteinase called chymase. In this study, we demonstrate that the inactive cytokine, 31 kD interleukin 1 beta (IL-1 beta), can be converted rapidly to an 18 kD biologically ac ...
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Journal ArticleLab Invest · October 1991
Human connective tissue type mast cells (CTMC) are frequently located in close proximity to microvascular and neural basement membranes (BM). We have explored the interaction between human dermal connective tissue-type (chymase positive) mast cells and lam ...
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Journal ArticleJ Invest Dermatol · June 1991
Mediators released from injured human skin that initiate the inflammatory response have not been adequately identified. Organ culture of full-thickness skin explants enables us to do so, because injury to the skin can be made in vitro, eliminating the rapi ...
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Journal ArticleArch Dermatol · April 1991
Methotrexate is an effective and convenient treatment for severe psoriasis whose use is limited by the development of hepatic fibrosis and cirrhosis in a small number of patients. The mechanism of hepatotoxicity is unknown, but it is believed to be the res ...
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Journal ArticleArch Dermatol · February 1991
Nine patients with adult-onset urticaria pigmentosa were studied for the incidence of extracutaneous mast cell involvement and the efficacy of potent topical corticosteroid therapy for cutaneous lesions. Seven of the nine patients had increased mast cells ...
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Journal ArticleJ Invest Dermatol · November 1990
Elevated levels of the serine proteinase plasminogen activator are observed in psoriatic lesions. In contrast to normal epidermis, lesional psoriatic epidermis contains primarily tissue type plasminogen activator (tPA) activity and much lower levels of uro ...
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Journal ArticleJ Invest Dermatol · November 1990
Plasminogen activator (PA), which catalyzes the conversion of plasminogen to the proteinase plasmin, has been implicated in a variety of cutaneous disorders. Lesional epidermis from patients with psoriasis, pemphigus, bullous pemphigoid, and Hailey-Hailey ...
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Journal ArticleJ Biol Chem · December 15, 1989
The ability of plasma proteinase inhibitors to inactivate human chymase, a chymotrypsin-like proteinase stored within mast cell secretory granules, was investigated. Incubation with plasma resulted in over 80% inhibition of chymase hydrolytic activity for ...
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Journal ArticleAm J Med · April 1989
In this report, we have presented our experience with a patient with a rare cutaneous granulocytic sarcoma. In addition to hematoxylin and eosin, myeloperoxidase stain and specific stains for lysozyme and esterase were helpful in confirming the histologic ...
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Journal ArticleJ Invest Dermatol · March 1989
To investigate the mechanisms by which cutaneous plasminogen activator (PA) may be regulated, we have tested cultured keratinocytes for the presence of PA inhibitors. Using biosynthetic labeling experiments with 35S-methionine in conjunction with specific ...
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Journal ArticleYale J Biol Med · 1989
Extracorporeal photochemotherapy is a new form of immunotherapy which involves the extracorporeal photoinactivation of peripheral blood cells by 8-methoxypsoralen in the presence of ultraviolet A irradiation, followed by readministration of the cells. To e ...
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Journal ArticleArch Dermatol · January 1989
This article summarizes the management of 22 cases of pyoderma gangrenosum over the past four years at the hospital of the University of Pennsylvania, Philadelphia. Eighteen patients with pyoderma gangrenosum were studied using the most sensitive routine l ...
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Journal ArticleJ Invest Dermatol · June 1988
To investigate the role of plasminogen activator (PA) in cutaneous disease, we have used biochemical and immunocytochemical techniques to examine PA in normal and lesional skin. In normal human dermis, tissue PA is the predominant PA activity; however, in ...
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Journal ArticleJ Invest Dermatol · June 1988
Urokinase-type plasminogen activator (uPA) is produced and secreted by cultured human keratinocytes as a single chain precursor. UPA in keratinocyte conditioned medium is not susceptible to inhibition with diisopropylfluorophosphate (DFP), and it has an ap ...
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Journal ArticleArch Biochem Biophys · April 1988
Serine class proteinases with trypsin-like and chymotrypsin-like specificity were purified from dog mastocytoma tissue. An antiserum was produced against the chymotrypsin-like proteinase. The antiserum reacted with mast cells in skin sections prepared from ...
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Journal ArticleJ Invest Dermatol · November 1987
Pemphigus IgG induces acantholysis in skin organ culture without the involvement of complement. Urokinase-type plasminogen activator, a proteolytic enzyme, has been implicated in the development of acantholysis. To test this hypothesis, we prepared a rabbi ...
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Journal ArticleJ Biol Chem · May 15, 1987
The subcellular localization of human skin chymase to mast cell granules was established by immunoelectron microscopy, and binding of chymase to the area of the dermo-epidermal junction, a basement membrane, was demonstrated immunocytochemically in cryosec ...
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Journal ArticleJ Invest Dermatol · April 1987
When confluent keratinocyte cultures were wounded by cutting with a blade, the cells rapidly retracted from the wounded site, leaving an area denuded of cells. Within 3-4 h of wounding, keratinocytes began to migrate from the edges and gradually reepitheli ...
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Journal ArticleDermatologica · 1987
Topical corticosteroids are utilized in the treatment of a wide variety of skin diseases, primarily those involving an inflammatory component. Recent investigations have revealed that one of the effects of long-term usage of steroids is the depletion of sk ...
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Journal ArticleArch Dermatol Res · 1986
The main chymotryptic and tryptic proteinases of human skin were found in high-salt extracts of human dermis. The levels of these enzymes were markedly increased in salt extracts of human cutaneous mastocytosis as compared to the levels found in extracts o ...
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Journal ArticleExp Cell Res · December 1985
Using biochemical and immunocytochemical approaches, we have investigated the plasminogen activator (PA) of primary human epidermal cell cultures. A rabbit antibody raised against human urinary PA (urokinase) inhibited greater than or equal to 96% of the P ...
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Journal ArticleArch Dermatol · December 1985
Based on a previous observation that the long-term application of potent topical corticosteroids under occlusion to normal skin resulted in the loss of mast cells, we investigated the effects of intralesional and topical steroids in urticaria pigmentosa (U ...
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Journal ArticleBiochemistry · April 9, 1985
The extended substrate binding sites of several chymotrypsin-like serine proteases, including rat mast cell proteases I and II (RMCP I and II, respectively) and human and dog skin chymases, have been investigated by using peptide 4-nitroanilide substrates. ...
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Journal ArticleJ Am Acad Dermatol · January 1985
In this report we describe the successful outcome following the use of corticosteroid pulse therapy in a patient with a severe phenytoin hypersensitivity reaction presenting with toxic epidermal necrolysis and severe hepatitis. Steroid pulse therapy may be ...
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Journal ArticleJ Invest Dermatol · November 1984
Human skin chymotrypsin-like proteinase, human neutrophil cathepsin G, rat mast cell chymase, and rat salivary gland tonin are cell-derived serine proteinases of similar size with specificity for amino acids of aromatic residues. Each enzyme was examined f ...
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Journal ArticleJ Exp Med · October 1, 1984
The degradation of normal human skin by the human polymorphonuclear leukocyte proteinases cathepsin G and elastase, and by a human skin chymotrypsin-like proteinase that appears to be a mast cell constituent, was examined. Enzymes were incubated with fresh ...
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Journal ArticleArch Dermatol · June 1984
The incubation of cultured epidermal cells with IgG obtained from a 56-year-old man with penicillamine-induced pemphigus resulted in an increase in extracellular and intracellular plasminogen activator. This suggests that penicillamine-induced pemphigus an ...
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Journal ArticleBr J Dermatol · March 1984
Human epidermal cells were cultured with pemphigus antibody in the presence of hydrocortisone, methylprednisolone sodium succinate, dapsone and gold. Hydrocortisone and methylprednisolone reduced the production of the enzyme plasminogen activator but dapso ...
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Journal ArticleJ Invest Dermatol · February 1984
A precise method for quantitation of polymorphonuclear leukocyte (PMNL) accumulation in skin in vivo, has been developed so that the proinflammatory effects of various agents can be compared. This method can also be used to evaluate the effect of therapeut ...
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Journal ArticleJ Invest Dermatol · September 1983
Psoriatic patients, particularly those with psoriatic arthritis, have neutrophilic and eosinophilic leukocytosis. Isolated polymorphonuclear leukocytes (PMNLs) from psoriatic patients have normal concentrations of proteolytic enzymes and they have beta-adr ...
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Journal ArticleArch Dermatol · August 1983
We describe two patients with pityriasis rubra pilaris in whom hypertriglyceridemia developed following the oral administration of high-dose vitamin A (retinol). The effects of transient hypertriglyceridemia are unknown, as both patients tolerated high-dos ...
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Journal ArticleBr J Dermatol · July 1983
The molecular sizes of secreted and cell-associated plasminogen activators from four cultured cell types were determined using an SDS-PAGE technique in which plasminogen and casein were included during polymerization of the polyacrylamide gel. The major ba ...
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Journal ArticleJ Invest Dermatol · June 1983
Involved psoriatic epidermis maintains its histologic appearance, increased labeling index, and increased level of plasminogen activator after grafting onto athymic nude mice. Epidermis from clinically uninvolved psoriatic skin develops an increase in plas ...
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Journal ArticleArch Dermatol · April 1983
A patient had cutaneous polyarteritis nodosa associated with Crohn's disease. Immunopathologic studies disclosed evidence of circulating immune complexes as measured by C1q binding. Immunofluorescent microscopy of lesional skin showed C3 and fibrin deposit ...
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Journal ArticleJ Biol Chem · March 10, 1983
A chymotrypsin-like proteinase was purified 2400-fold from human skin. The procedure involves extraction of the proteinase from skin in 2 M KCl, precipitation with protamine chloride, fractionation by gel filtration chromatography, and fractionation by chr ...
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Journal ArticleJ Exp Med · January 1, 1983
Binding of anti-cell surface pemphigus autoantibodies to cultured human epidermal cells stimulates synthesis and secretion of plasminogen activator (PA). Increases in PA activity were detected within 6 h of the addition of IgG and stimulation was dependent ...
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Journal ArticleBr J Dermatol · January 1983
The specific activity of plasminogen activator was increased in clinically involved psoriatic epidermis compared with the uninvolved skin of the same eight patients. Alterations in plasminogen activator activity correlated with disease activity as measured ...
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Journal ArticleJ Am Acad Dermatol · January 1983
Atrophie blanche vasculitis is due to thromboocclusion of dermal blood vessels. A 28-year-old white man with a very severe case of this disease had a complete remission when he was treated with minidose heparin sodium injections. As little as 5,000 U of he ...
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Journal ArticleJ Invest Dermatol · December 1982
Triton X-100 extracts of cultured human epidermal cells exhibited proteolytic activity as measured by the hydrolysis of [3H]-casein at neutral pH. The majority of endogenous proteolytic activity was inhibited by parahydroxy mercuribenzoate and by mersalyl ...
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Journal ArticleArch Dermatol · July 1982
Aberrations of cellular immune functions in pyoderma gangrenosum (PG) may lead to nonspecific activation of inflammatory cells or to an imbalance of suppression leading to autoaggression (chronic ulceration). A patient with severe unremitting PG had anergy ...
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Journal ArticleJ Immunol · March 1982
Incubation of monolayer cultures of human melanoma cells with monkey anti-human melanoma IgG resulted in loss of cellular adhesion. Release of melanoma cells from the culture dish was not the result of cytotoxicity. Antibody-induced cell detachment was par ...
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Journal ArticleScience · February 5, 1982
Clinically involved psoriatic epidermis maintains its histological appearance, increased labeling index, and increased level of plasminogen activator after being grafted onto athymic nude mice. Uninvolved psoriatic epidermis develops increases in plasminog ...
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Journal ArticleSpringer Semin Immunopathol · June 1981
The current state of understanding of pemphigus includes the following: 1. Pemphigus is an autoimmune disease. In all variants a circulating autoantibody is found which binds to epidermal cells. In vivo antibody may be found deposited in the epidermis of p ...
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Journal ArticleJ Invest Dermatol · August 1980
Five patients with Darier's disease and 6 patients with pityriasis rubra pilaris were treated with 13-cis-retinoic acid. Extracts of separated epidermis were assayed for extractable protein, lactic dehydrogenase, Cathepsin D, beta glucuronidase and neutral ...
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Journal ArticleJ Histochem Cytochem · July 1980
A monospecific antibody to purified human skin chemotactic proteinase was raised in rabbits and shown to produce a single line of identity against crude and purified human proteinase. The antibody was used to localize the proteinase in human fibroblasts, p ...
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Journal ArticleJ Invest Dermatol · May 1980
An in vitro model system using cultured newborn epidermal cells was employed to investigate the binding of pemphigus autoantibody and subsequent loss of adhesion between epidermal cells. Pemphigus antibodies bound to both mouse and human cultured epidermal ...
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Journal ArticleCutis · April 1980
A new synthetic oral retinoid, 13-cis retinoic acid, is fairly well tolerated in patients and appears to be effective in those with Darier's disease and lamellar ichthyosis. It is less effective in those with pityriasis rubra pilaris. The mechanism of acti ...
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Journal ArticleJ Surg Oncol · 1979
A possible mechanism for tumor cell invasion of normal tissue might be secretion of proteolytic enzymes. This study compares and contrasts production and secretion of proteinases by cell cultures of normal and chemically transformed mouse epithelial cells. ...
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Journal ArticleJ Immunol · February 1978
A proteinase active at physiologic pH was isolated from unstimulated human peripheral blood lymphocytes with gel filtration and affinity chromatography. The proteinase with a molecular mass of approximately 30,000 daltons was completely inhibited by diisop ...
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Journal ArticleProc Natl Acad Sci U S A · January 1978
Immunoglobulin from pemphigus patients binds to the surface of mouse epidermal cells in culture. Cells incubated with the pemphigus antibody are easily detached from culture plates whereas cells incubated with serum from normal patients remain on the plate ...
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Journal ArticleScience · December 16, 1977
Extracts of involved and uninvolved skin from nine patients with untreated psoriasis were studied for chemotactic activity. Psoriatic plaque contains increased amounts of a complement-dependent chemotactic factor that is inhibited by diisopropyl fluorophos ...
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Journal ArticleBiochim Biophys Acta · July 8, 1977
A proteinase (EC 3.4.-.-) active at physiological pH has been isolated from human skin utilizing gel filtration and affinity chromatography techniques. The proteinase has a molecular weight of approx. 28 000 and it is inhibited by alpha 2-macroglobulin, al ...
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Journal ArticleBr J Dermatol · January 1977
A patient with epidermolysis bullosa hereditaria letalis had the characteristic electron microscopic lesions not only in the skin, but also in the gastro-intestinal, genito-urinary and respiratory tracts. Administration of dexamethasone to this patients re ...
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Journal ArticleBiochim Biophys Acta · December 8, 1976
Three neutral proteinases (EC 3.4.--.--) and cathepsin D have been identified in human epidermis utilizing a highly sensitive radioactive method. The proteinases were extracted in 1.0 M KC1 and 0.1% Triton X-100 and separated by Sephadex G-75 chromatograph ...
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Journal ArticleArch Dermatol · July 1976
A patient with acute pancreatitis developed subcutaneous fat necrosis of the anterior abdominal wall secondary to leakage of pancreatic enzymes through a rent in the peritoneum following paracentesis. The same patient also had another subcutaneous complica ...
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Journal ArticleArch Dermatol · September 1975
The lysosomal proteinase cathepsin D has been localized in rabbit skin by immunocytochemical techniques. The enzyme was found in the basal layer of the epidermis, hair follicles, sebaceous glands, fibroblasts, and endothelial cells. An autoradiographic tec ...
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Journal ArticleBiochem J · February 1974
1. Experiments were made to determine whether the purified lysosomal proteinases, cathepsins B1 and D, degrade acid-soluble collagen in solution, reconstituted collagen fibrils, insoluble collagen or gelatin. 2. At acid pH values cathepsin B1 released (14) ...
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Journal ArticleJ Exp Med · May 1, 1973
Specific anti-(rabbit cathepsin D) serum, previously shown to inhibit cathepsin D, arrested the intracellular digestion of sheep IgG and radiochemically labeled hemoglobin and proteoglycan in rabbit alveolar macrophages. In the presence of antiserum, cells ...
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Journal ArticleJ Clin Invest · December 1968
This report suggests a mechanism for collagen degradation mediated by human granulocytic leukocytes. A specific collagenase, which is extractable from human granulocytes, has been partially purified by DEAE chromatography. This collagenolytic enzyme is ope ...
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Journal ArticleScience · March 29, 1968
A collagenase, operative at neutral and alkaline pH, has been extracted from the granule fraction of human granulocytic leukocytes. It digests reconstituted collagen fibrils and reduces the viscosity of collagen solutions. Cleavage of collagen in solution ...
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