Scholars@Duke
Fan Yuan

Fan Yuan

Professor of Biomedical Engineering
Biomedical Engineering
fyuan@duke.edu
Duke Box 90281, Durham, NC 27708-0281
Rm 1385, Fciemas, Duke Univers, Durham, NC 27708-0281

Selected Publications

Spatiotemporal Control of Immune Responses with Nucleic Acid Cocktail Vaccine

Journal Article Advanced Therapeutics · November 1, 2024 Nucleic acid vaccines play important roles in the prevention and treatment of diseases. However, limited immunogenicity remains a major obstacle for DNA vaccine applications in the clinic. To address the issue, the present study investigates a cocktail app ... Full text Cite

A comprehensive comparison of DNA and RNA vaccines.

Journal Article Advanced drug delivery reviews · July 2024 Nucleic acid technology has revolutionized vaccine development, enabling rapid design and production of RNA and DNA vaccines for prevention and treatment of diseases. The successful deployment of mRNA and plasmid DNA vaccines against COVID-19 has further v ... Full text Cite

Sucrose Treatment Enhances the Electrotransfer of DNA by Activating Phospholipase A2.

Journal Article Pharmaceutics · March 29, 2024 Our previous study discovered that sucrose and other non-reducing sugars (e.g., trehalose and raffinose) could be used to improve the electrotransfer (ET) of molecular cargo, including DNA, mRNA, and ribonucleoprotein in various cell lines and primary huma ... Full text Link to item Cite

Nuclear Entry of DNA and Transgene Expression in Dividing and Non-dividing Cells.

Journal Article Cellular and molecular bioengineering · December 2023 IntroductionPlasmid DNA (pDNA) must be delivered into the nucleus for transgene expression in mammalian cells. The entry may happen passively during the nuclear envelope breakdown and reformation in dividing cells or actively through the nuclear p ... Full text Cite

Transient inhibition of lysosomal functions potentiates nucleic acid vaccines.

Journal Article Proc Natl Acad Sci U S A · October 31, 2023 Nucleic acid vaccines have shown promising results in the clinic against infectious diseases and cancers. To robustly improve the vaccine efficacy and safety, we developed an approach to increase the intracellular stability of nucleic acids by transiently ... Full text Link to item Cite

Copy number of naked DNA delivered into nucleus of mammalian cells by electrotransfection.

Journal Article Bioelectrochemistry (Amsterdam, Netherlands) · October 2023 Electrotransfection is a non-viral method for delivery of nucleic acids into cells. In our previous study, we have determined the minimal copy number of plasmid DNA (pDNA) per cell required for transgene expression post electrotransfection, and developed a ... Full text Cite

Power-controlled acoustofluidic manipulation of microparticles.

Journal Article Ultrasonics · September 2023 Recently, surface acoustic wave (SAW) based acoustofluidic separation of microparticles and cells has attracted increasing interest due to accuracy and biocompatibility. Precise control of the input power of acoustofluidic devices is essential for generati ... Full text Cite

Modelling hybrid acoustofluidic devices for enhancing Nano- and Micro-Particle manipulation in microfluidics

Journal Article Applied Acoustics · March 30, 2023 Acoustofluidic techniques are increasingly used to manipulate nano- and micro-particles in microfluidics. A wide range of acoustofluidic devices consisting of microchannels and acoustic sources have been developed for applications in biochemistry and biome ... Full text Cite

A Double-Permeability Poroelasticity Model for Fluid Transport in a Biological Tissue

Journal Article Transport in Porous Media · March 1, 2023 This work presents a double-permeability poroelasticity model for fluid flows in both the microvascular and interstitial networks in a biological tissue. In the newly developed model, both networks are modeled as porous structures with distinct permeabilit ... Full text Cite

Nanoenhancer for improving naked DNA electrotransfection In vivo.

Journal Article Frontiers in bioengineering and biotechnology · January 2023 Introduction: Electrotransfection (ET) is a non-viral approach widely used for delivery of naked nucleic acids. Its efficiency can be increased in vitro by treatment of cells with various small molecule enhancers. However, these enhancers oft ... Full text Cite

A statistical framework for determination of minimal plasmid copy number required for transgene expression in mammalian cells.

Journal Article Bioelectrochemistry (Amsterdam, Netherlands) · April 2021 Plasmid DNA (pDNA) has been widely used for non-viral gene delivery. After pDNA molecules enter a mammalian cell, they may be trapped in subcellular structures or degraded by nucleases. Only a fraction of them can function as templates for transcription in ... Full text Cite

An Enhanced Tilted-Angle Acoustofluidic Chip for Cancer Cell Manipulation

Journal Article IEEE Electron Device Letters · April 1, 2021 In recent years, surface acoustic wave (SAW) devices have demonstrated great potentials and increasing applications in the manipulation of nano- and micro-particles including biological cells with the advantages of label-free, high sensitivity and accuracy ... Full text Cite

Thin film Gallium nitride (GaN) based acoustofluidic Tweezer: Modelling and microparticle manipulation.

Journal Article Ultrasonics · December 2020 Gallium nitride (GaN) is a compound semiconductor which shows advantages in new functionalities and applications due to its piezoelectric, optoelectronic, and piezo-resistive properties. This study develops a thin film GaN-based acoustic tweezer (GaNAT) us ... Full text Cite

The Long Noncoding RNA NEAT1 Promotes Sarcoma Metastasis by Regulating RNA Splicing Pathways.

Journal Article Mol Cancer Res · October 2020 Soft-tissue sarcomas (STS) are rare malignancies showing lineage differentiation toward diverse mesenchymal tissues. Half of all high-grade STSs develop lung metastasis with a median survival of 15 months. Here, we used a genetically engineered mouse model ... Full text Link to item Cite

Inhibition of Caspases Improves Non-Viral T Cell Receptor Editing.

Journal Article Cancers · September 2020 T cell receptor (TCR) knockout is a critical step in producing universal chimeric antigen receptor T cells for cancer immunotherapy. A promising approach to achieving the knockout is to deliver the CRISPR/Cas9 system into cells using electrotransfer techno ... Full text Cite

Enhancing Cell Viability and Efficiency of Plasmid DNA Electrotransfer Through Reducing Plasma Membrane Permeabilization.

Journal Article Bioelectricity · September 2020 BackgroundPulsed electric field has been widely used to facilitate molecular cargo transfer into cells. However, the cell viability is often decreased when trying to increase the electrotransfer efficiency. We hypothesize that the decrease is due ... Full text Cite

Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo.

Journal Article Adv Biosyst · August 2020 Cell engineering relies heavily on viral vectors for the delivery of molecular cargo into cells due to their superior efficiency compared to nonviral ones. However, viruses are immunogenic and expensive to manufacture, and have limited delivery capacity. N ... Full text Link to item Cite

Correction: Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB.

Journal Article Lab on a chip · August 2020 Correction for 'Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB' by Roman Mikhaylov et al., Lab Chip, 2020, 20, 1807-1814, DOI: 10.1039/C9LC01192G. ... Full text Cite

Gallium Nitride: A Versatile Compound Semiconductor as Novel Piezoelectric Film for Acoustic Tweezer in Manipulation of Cancer Cells

Journal Article IEEE Transactions on Electron Devices · August 1, 2020 Gallium nitride (GaN) is a compound semiconductor which has advantages to generate new functionalities and applications due to its piezoelectric, pyroelectric, and piezo-resistive properties. Recently, surface acoustic wave (SAW)-based acoustic tweezers we ... Full text Cite

Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB.

Journal Article Lab on a chip · May 2020 Acoustofluidics has been increasingly applied in biology, medicine and chemistry due to its versatility in manipulating fluids, cells and nano-/micro-particles. In this paper, we develop a novel and simple technology to fabricate a surface acoustic wave (S ... Full text Cite

Ultrastructural Analysis of Vesicular Transport in Electrotransfection.

Journal Article Microsc Microanal · October 2018 Emerging evidence from various studies indicates that plasmid DNA (pDNA) is internalized by cells through an endocytosis-like process when it is used for electrotransfection. To provide morphological evidence of the process, we investigated ultrastructures ... Full text Link to item Cite

Current Progress in Electrotransfection as a Nonviral Method for Gene Delivery.

Journal Article Molecular pharmaceutics · September 2018 Electrotransfection (ET) is a nonviral method for delivery of various types of molecules into cells both in vitro and in vivo. Close to 90 clinical trials that involve the use of ET have been performed, and approximately half of them are related to cancer ... Full text Cite

Quantum confined peptide assemblies with tunable visible to near-infrared spectral range.

Journal Article Nature communications · August 2018 Quantum confined materials have been extensively studied for photoluminescent applications. Due to intrinsic limitations of low biocompatibility and challenging modulation, the utilization of conventional inorganic quantum confined photoluminescent materia ... Full text Cite

Enhancing Electrotransfection Efficiency through Improvement in Nuclear Entry of Plasmid DNA.

Journal Article Molecular therapy. Nucleic acids · June 2018 The nuclear envelope is a physiological barrier to electrogene transfer. To understand different mechanisms of the nuclear entry for electrotransfected plasmid DNA (pDNA), the current study investigated how manipulation of the mechanisms could affect elect ... Full text Cite

1213 Exosome-mediated RNAi delivery for melanoma therapy

Conference Journal of Investigative Dermatology · May 2018 Full text Cite

The brain interstitial system: Anatomy, modeling, in vivo measurement, and applications.

Journal Article Progress in neurobiology · October 2017 Although neurons attract the most attention in neurobiology, our current knowledge of neural circuit can only partially explain the neurological and psychiatric conditions of the brain. Thus, it is also important to consider the influence of brain intersti ... Full text Cite

Stiffness characterization of anisotropic trabecular meshwork.

Journal Article Journal of biomechanics · August 2017 Elevation of intraocular pressure has been correlated to changes in stiffness of trabecular meshwork (TM) in glaucomatous eyes although mechanical properties of the TM remain to be quantitatively determined. Data in the literature suggest that the TM canno ... Full text Cite

Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection.

Journal Article Mol Ther · March 1, 2017 Electrotransfection is a widely used method for delivering genes into cells with electric pulses. Although different hypotheses have been proposed, the mechanism of electrotransfection remains controversial. Previous studies have indicated that uptake and ... Full text Link to item Cite

Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection.

Journal Article PloS one · January 2017 A recent theory suggests that endocytosis is involved in uptake and intracellular transport of electrotransfected plasmid DNA (pDNA). The goal of the current study was to understand if approaches used previously to improve endocytosis of gene delivery vect ... Full text Cite

Nuclear Envelope As A Physical Barrier In Electrotransfection.

Conference MOLECULAR BIOLOGY OF THE CELL · January 1, 2017 Link to item Cite

Improvement in Electrotransfection of Cells Using Carbon-Based Electrodes.

Journal Article Cellular and molecular bioengineering · December 2016 Electrotransfection has been widely used as a versatile, non-viral method for gene delivery. However, electrotransfection efficiency (eTE) is still low and unstable, compared to viral methods. To understand potential mechanisms of the unstable eTE, we inve ... Full text Cite

Visualization of conventional outflow tissue responses to netarsudil in living mouse eyes.

Journal Article Eur J Pharmacol · September 15, 2016 Visual impairment due to glaucoma currently impacts 70 million people worldwide. While disease progression can be slowed or stopped with effective lowering of intraocular pressure, current medical treatments are often inadequate. Fortunately, three new cla ... Full text Link to item Cite

Elastic hydrogel as a sensor for detection of mechanical stress generated by single cells grown in three-dimensional environment.

Journal Article Biomaterials · August 2016 Cell volume growth occurs in all living tissues. The growth exerts mechanical stresses on surrounding tissues that may alter tissue microenvironment, and have significant implications in health and diseases. However, the level of growth stress generated by ... Full text Cite

Mathematical Modeling of Outflow Facility Increase With Trabecular Meshwork Bypass and Schlemm Canal Dilation.

Journal Article J Glaucoma · April 2016 PURPOSE: To mathematically model the conventional aqueous humor outflow system with trabecular meshwork (TM) bypass and Schlemm canal (SC) dilation. METHODS: The SC was modeled as a rectangular channel with the TM modeled as a permeable membrane. The colle ... Full text Link to item Cite

Mechanical analysis of rat trabecular meshwork.

Journal Article Soft matter · April 2015 Stiffness of trabecular meshwork (TM) may play an important role in regulating outflow resistance in healthy and glaucomatous eyes. However, the current techniques for stiffness measurement can only be applied to TM dissected from human donor or large anim ... Full text Cite

A power-law dependence of bacterial invasion on mammalian host receptors.

Journal Article PLoS computational biology · April 2015 Pathogenic bacteria such as Listeria and Yersinia gain initial entry by binding to host target cells and stimulating their internalization. Bacterial uptake entails successive, increasingly strong associations between receptors on the surface of bacteria a ... Full text Cite

Endothelial Cell Senescence Increases Traction Forces due to Age-Associated Changes in the Glycocalyx and SIRT1.

Journal Article Cellular and molecular bioengineering · March 2015 Endothelial cell (EC) aging and senescence are key events in atherogenesis and cardiovascular disease development. Age-associated changes in the local mechanical environment of blood vessels have also been linked to atherosclerosis. However, the extent to ... Full text Cite

A self-adaptive sampling digital image correlation algorithm for accurate displacement measurement

Journal Article Optics and Lasers in Engineering · February 1, 2015 Digital image correlation (DIC) is nowadays widely applied to many engineering areas as an effective optical displacement measurement technique. To minimize the potential effect of spatial sampling locations on full-field displacement measurement, this pap ... Full text Cite

Macrophage embedded fibrin gels: an in vitro platform for assessing inflammation effects on implantable glucose sensors.

Journal Article Biomaterials · December 2014 The erroneous and unpredictable behavior of percutaneous glucose sensors just days following implantation has limited their clinical utility for diabetes management. Recent research has implicated the presence of adherent inflammatory cells as the key miti ... Full text Cite

Circumferential tensile stiffness of glaucomatous trabecular meshwork.

Journal Article Invest Ophthalmol Vis Sci · February 10, 2014 PURPOSE: Our previous work indicated that a larger circumferential Young's modulus (E) of trabecular meshwork (TM) correlated with a higher outflow facility (C) in normal human donor eyes. The current study investigated the influence of glaucomatous TM sti ... Full text Link to item Cite

Mathematical modeling of the Phoenix Rising pathway.

Journal Article PLoS Comput Biol · February 2014 Apoptosis is a tightly controlled process in mammalian cells. It is important for embryogenesis, tissue homoeostasis, and cancer treatment. Apoptosis not only induces cell death, but also leads to the release of signals that promote rapid proliferation of ... Full text Link to item Cite

Disease progression in iridocorneal angle tissues of BMP2-induced ocular hypertensive mice with optical coherence tomography.

Journal Article Mol Vis · 2014 PURPOSE: The goal of the present study was to test for the first time whether glaucomatous-like disease progression in a mouse can be assessed morphologically and functionally with spectral domain optical coherence tomography (SD-OCT). METHODS: We monitore ... Link to item Cite

Role of specific endocytic pathways in electrotransfection of cells.

Journal Article Molecular therapy. Methods & clinical development · January 2014 Electrotransfection is a technique utilized for gene delivery in both preclinical and clinical studies. However, its mechanisms are not fully understood. The goal of this study was to investigate specific pathways of endocytosis involved in electrotransfec ... Full text Cite

Accurate displacement measurement via a self-adaptive digital image correlation method based on a weighted ZNSSD criterion

Journal Article Optics and Lasers in Engineering · January 1, 2014 Digital image correlation (DIC) technique has been increasingly employed to implement surface deformation measurements in many engineering fields. Practically, it has been demonstrated that the choice of subset sizes exerts a strong influence on measuremen ... Full text Cite

Predicting glucose sensor behavior in blood using transport modeling: relative impacts of protein biofouling and cellular metabolic effects.

Journal Article Journal of diabetes science and technology · November 2013 BackgroundTissue response to indwelling glucose sensors remains a confounding barrier to clinical application. While the effects of fully formed capsular tissue on sensor response have been studied, little has been done to understand how tissue in ... Full text Cite

Circumferential Trabecular Meshwork Stiffness in Glaucomatous Eyes

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · June 1, 2013 Link to item Cite

Local Stiffness of Rat Trabecular Meshwork

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · June 1, 2013 Link to item Cite

Digital Image Correlation with Self-Adaptive Gaussian Windows

Journal Article Experimental Mechanics · March 1, 2013 A novel subpixel registration algorithm with Gaussian windows is put forward for accurate deformation measurement in digital image correlation technique. Based on speckle image quality and potential deformation states, this algorithm can automatically mini ... Full text Cite

Improving interstitial transport of macromolecules through reduction in cell volume fraction in tumor tissues.

Journal Article Nanomedicine : nanotechnology, biology, and medicine · October 2012 Interstitial transport of large molecules and nanoparticles is an important concern in nanomedicine-mediated cancer treatment. To that end, the current study was proposed to improve the transport through enlargement of extracellular space by treating tumor ... Full text Cite

A novel Schlemm's Canal scaffold increases outflow facility in a human anterior segment perfusion model.

Journal Article Investigative ophthalmology & visual science · September 2012 PurposeAn intracanalicular scaffold (Hydrus microstent) designed to reduce intraocular pressure as a glaucoma treatment was tested in human anterior segments to determine changes in outflow facility (C).MethodsHuman eyes with no history o ... Full text Cite

Differential effects of trabecular meshwork stiffness on outflow facility in normal human and porcine eyes.

Journal Article Invest Ophthalmol Vis Sci · August 9, 2012 PURPOSE: The study was designed to determine trabecular meshwork (TM) stiffness and its relationship to outflow facility (C) in perfused normal human and porcine eyes. METHODS: Human and porcine eyes were perfused at pressures of 10, 20, 30, and 40 mm Hg t ... Full text Link to item Cite

Critical issues in delivery of RNAi therapeutics in vivo.

Journal Article Current pharmaceutical biotechnology · June 2012 RNA interference (RNAi) is a fundamental mechanism of gene regulation and has been harnessed to produce a new class of drugs for treatment of various diseases. A key issue in these applications is how to effectively deliver RNAi therapeutics into target ce ... Full text Cite

A microfluidic system for investigation of extravascular transport and cellular uptake of drugs in tumors.

Journal Article Biotechnology and bioengineering · May 2012 Three-dimensional (3D) tumor models have been established in various microfluidic systems for drug delivery and resistance studies in vitro. However, one of the main drawbacks of these models is non-uniform distribution of cells, leaving regions with very ... Full text Cite

Regulation of trabecular meshwork cell contraction and intraocular pressure by miR-200c.

Journal Article PLoS One · 2012 Lowering intraocular pressure (IOP) delays or prevents the loss of vision in primary open-angle glaucoma (POAG) patients with high IOP and in those with normal tension glaucoma showing progression. Abundant evidence demonstrates that inhibition of contract ... Full text Link to item Cite

Special issue: Experiments and modeling in microand nano-biomechanics

Journal Article Cellular and Molecular Bioengineering · September 1, 2011 Full text Cite

Proliferation behavior of E. coli in a three-dimensional in vitro tumor model.

Journal Article Integrative biology : quantitative biosciences from nano to macro · June 2011 Advances in genetic engineering of non-pathogenic Escherichia coli (E. coli) have made this organism an attractive candidate for gene delivery vehicle. However, proliferation and transport behaviors of E. coli in three-dimensional (3D) tumor environment ar ... Full text Cite

Cellular pharmacokinetic and pharmacodynamic analyses of ethacrynic acid: Implications in topical drug delivery in the eye.

Journal Article Mol Vis · 2011 PURPOSE: Ethacrynic acid (ECA) is a potential trabecular meshwork (TM) drug that has shown promising results in preclinical studies for treatment of primary open-angle glaucoma. However, topical application of ECA is currently limited by adverse effects in ... Link to item Cite

Membrane binding of plasmid DNA and endocytic pathways are involved in electrotransfection of mammalian cells.

Journal Article PloS one · January 2011 Electric field mediated gene delivery or electrotransfection is a widely used method in various studies ranging from basic cell biology research to clinical gene therapy. Yet, mechanisms of electrotransfection are still controversial. To this end, we inves ... Full text Open Access Cite

A review of three-dimensional in vitro tissue models for drug discovery and transport studies.

Journal Article Journal of pharmaceutical sciences · January 2011 The use of animal models in drug discovery studies presents issues with feasibility and ethical concerns. To address these limitations, in vitro tissue models have been developed to provide a means for systematic, repetitive, and quantitative investigation ... Full text Cite

Enhancement of electric field-mediated gene delivery through pretreatment of tumors with a hyperosmotic mannitol solution.

Journal Article Cancer gene therapy · January 2011 Pulsed electric fields can enhance interstitial transport of plasmid DNA (pDNA) in solid tumors. However, the extent of enhancement is still limited. To this end, the effects of cellular resistance to electric field-mediated gene delivery were investigated ... Full text Cite

Modeling the relative impact of capsular tissue effects on implanted glucose sensor time lag and signal attenuation.

Journal Article Analytical and bioanalytical chemistry · October 2010 Little is known mechanistically about why implanted glucose sensors lag behind blood glucose levels in both the time to peak sensor response and the magnitude of peak sensor response. A mathematical model of glucose transport from capillaries through surro ... Full text Cite

Numerical simulations of ethacrynic acid transport from precorneal region to trabecular meshwork.

Journal Article Annals of biomedical engineering · March 2010 Topical application of drugs for treatment of intraocular diseases is often limited by inadequate transport and induced toxicity in corneal tissues. To improve the drug delivery, a mathematical model was developed to numerically simulate the transport proc ... Full text Cite

Comparative effects of thermosensitive doxorubicin-containing liposomes and hyperthermia in human and murine tumours.

Journal Article Int J Hyperthermia · 2010 PURPOSE: In previous reports, laboratory-made lysolecithin-containing thermosensitive liposome encapsulating doxorubicin (LTSL-DOX) showed potent anticancer effects in FaDu human squamous cell carcinoma. To further study the spectrum of LTSL-DOX activity, ... Full text Link to item Cite

Tumor microvascular permeability is a key determinant for antivascular effects of doxorubicin encapsulated in a temperature sensitive liposome.

Journal Article Int J Hyperthermia · September 2008 Previous data have demonstrated that doxorubicin (DOX) released from a lysolecithin-containing thermosensitive liposome (LTSL) can shut down blood flow in a human tumor xenograft (FaDu) in mice when the treatment is combined with hyperthermia (HT), suggest ... Full text Link to item Cite

Relaxin treatment of solid tumors: effects on electric field-mediated gene delivery.

Journal Article Molecular cancer therapeutics · August 2008 Pulsed electric fields have been shown to enhance interstitial transport of plasmid DNA (pDNA) in solid tumors in vivo. However, the extent of enhancement is still limited partly due to the collagen component in extracellular matrix. To this end, effects o ... Full text Cite

Field distribution and DNA transport in solid tumors during electric field-mediated gene delivery.

Journal Article Journal of pharmaceutical sciences · February 2008 Gene therapy has a great potential in cancer treatment. However, the efficacy of cancer gene therapy is currently limited by the lack of a safe and efficient means to deliver therapeutic genes into the nucleus of tumor cells. One method under investigation ... Full text Cite

Dose response of angiogenesis to basic fibroblast growth factor in rat corneal pocket assay: II. Numerical simulations.

Journal Article Microvascular research · January 2008 Angiogenesis involves interactions among various molecules and cells. To understand the complexity of interactions, we developed a mathematical model to numerically simulate angiogenesis induced by basic fibroblast growth factor (bFGF) in the corneal pocke ... Full text Cite

Effects of transmural fluid exchange on tumor blood flow

Journal Article BIORHEOLOGY · January 1, 2008 Link to item Cite

Mechanistic analysis of electroporation-induced cellular uptake of macromolecules.

Journal Article Experimental biology and medicine (Maywood, N.J.) · January 2008 Pulsed electric field has been widely used as a nonviral gene delivery platform. The delivery efficiency can be improved through quantitative analysis of pore dynamics and intracellular transport of plasmid DNA. To this end, we investigated mechanisms of c ... Full text Cite

Dose response of angiogenesis to basic fibroblast growth factor in rat corneal pocket assay: I. Experimental characterizations.

Journal Article Microvascular research · January 2008 Understanding mechanisms of formation of vascular networks under different experimental conditions is essential for improving treatment of angiogenesis-dependent diseases. To this end, we investigated the dose response of angiogenesis to basic fibroblast g ... Full text Cite

Electric field-mediated transport of plasmid DNA in tumor interstitium in vivo.

Journal Article Bioelectrochemistry (Amsterdam, Netherlands) · November 2007 Local pulsed electric field application is a method for improving non-viral gene delivery. Mechanisms of the improvement include electroporation and electrophoresis. To understand how electrophoresis affects pDNA delivery in vivo, we quantified the magnitu ... Full text Cite

Electric fields around and within single cells during electroporation-a model study.

Journal Article Annals of biomedical engineering · July 2007 One of the key issues in electric field-mediated molecular delivery into cells is how the intracellular field is altered by electroporation. Therefore, we simulated the electric field in both the extracellular and intracellular domains of spherical cells d ... Full text Cite

Transscleral diffusion of ethacrynic acid and sodium fluorescein.

Journal Article Mol Vis · February 22, 2007 PURPOSE: One of the current limitations in developing novel glaucoma drugs that target the trabecular meshwork (TM) is the induced corneal toxicity from eyedrop formulations. To avoid the corneal toxicity, an alternative approach would be to deliver TM dru ... Link to item Cite

Engineering novel synthetic biological systems

Journal Article IET Synthetic Biology · 2007 Cite

Glucose recovery with bare and hydrogel-coated microdialysis probes: experiment and simulation of temporal effects.

Journal Article Analytical chemistry · January 2007 In vitro microdialysis glucose sampling was used to test the transient and steady-state suitability of antifouling hydrogel coatings, composed of 2-hydroxyethyl methacrylate, vinylpyrrolidinone, and poly(ethylene glycol). The in vitro glucose diffusion coe ... Full text Cite

A single molecule detection method for understanding mechanisms of electric field-mediated interstitial transport of genes.

Journal Article Bioelectrochemistry (Amsterdam, Netherlands) · October 2006 The interstitial space is a rate limiting physiological barrier to non-viral gene delivery. External pulsed electric fields have been proposed to increase DNA transport in the interstitium, thereby improving non-viral gene delivery. In order to characteriz ... Full text Cite

Electric fields in tumors exposed to external voltage sources: implication for electric field-mediated drug and gene delivery.

Journal Article Annals of biomedical engineering · October 2006 The intratumoral field, which determines the efficiency of electric field-mediated drug and gene delivery, can differ significantly from the applied field. Therefore, we investigated the distribution of the electric field in mouse tumors and tissue phantom ... Full text Cite

Preferential extravasation and accumulation of liposomal vincristine in tumor comparing to normal tissue enhances antitumor activity.

Journal Article Cancer Chemother Pharmacol · August 2006 To quantitatively evaluate the extravasation, accumulation and selectivity to tumor tissues of liposomal vincristine (LV), dorsal skin-fold window chambers on athymic mice with or without LX-1, a human small cell lung cancer, xenograft implants and fluores ... Full text Link to item Cite

Nonlinear dependence of hydraulic conductivity on tissue deformation during intratumoral infusion.

Journal Article Annals of biomedical engineering · July 2006 Efficiency of intratumoral infusion for drug and gene delivery depends on intrinsic tissue structures as well as infusion-induced changes in these structures. To this end, we investigated effects of infusion pressure (P(inf)) and infusion-induced tissue de ... Full text Cite

Hyperthermia mediated liposomal drug delivery.

Journal Article Int J Hyperthermia · May 2006 Drug delivery systems have been developed for cancer therapy in an attempt to increase the tumour drug concentration while limiting systemic exposure. Liposomes have achieved passive targeting of solid tumours through enhanced vascular permeability, which ... Full text Link to item Cite

Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers.

Journal Article J Natl Cancer Inst · March 1, 2006 BACKGROUND: Delivery of anticancer therapeutic agents to solid tumors is problematic. Macromolecular drug carriers are an attractive alternative drug delivery method because they appear to target tumors and have limited toxicity in normal tissues. We inves ... Full text Link to item Cite

Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery.

Journal Article Mol Cancer Ther · February 2006 Recent studies have shown that up to 90% of viral vectors could disseminate to normal organs following intratumoral infusion. The amount of dissemination might be dependent on the infusion conditions. Therefore, we investigated the effects of infusion rate ... Full text Link to item Cite

Delivery of viral vectors to tumor cells: extracellular transport, systemic distribution, and strategies for improvement.

Journal Article Annals of biomedical engineering · January 2006 It is a challenge to deliver therapeutic genes to tumor cells using viral vectors because (i) the size of these vectors are close to or larger than the space between fibers in extracellular matrix and (ii) viral proteins are potentially toxic in normal tis ... Full text Cite

Quantitative comparison of the inhibitory effects of GW5638 and tamoxifen on angiogenesis in the cornea pocket assay.

Journal Article Angiogenesis · 2006 GW5638 is a novel tissue-selective estrogen receptor (ER) modulator. Structurally, it is a derivative of tamoxifen that is known for its inhibitory effects on angiogenesis in an ER-independent manner. Therefore, it is possible that GW5638 has the same effe ... Full text Link to item Cite

Ex vivo and in vivo mapping of electric fields in tumor tissues

Journal Article Proceedings of the 2005 Summer Bioengineering Conference · December 1, 2005 Electric field-mediated gene delivery is a promising therapeutic modality that uses an externally applied voltage source to introduce exogenous genetic materials to target cells by field-dependent phenomena, such as electrophoresis and electroporation. In ... Cite

In vivo electric field-mediated transport of plasmid DNA in tumor interstitium

Journal Article Proceedings of the 2005 Summer Bioengineering Conference · December 1, 2005 Cite

Numerical simulations of transcorneal tranport of ethacrynic acid

Journal Article Proceedings of the 2005 Summer Bioengineering Conference · December 1, 2005 Potential trabecular meshwork (TM) drugs can be delivered with a lower concentration on the cornea surface over a long period of time for reducing the induced corneal toxicity. To determine the concentration of drugs that can be achieved in the TM, we deve ... Cite

A novel method for viral gene delivery in solid tumors.

Journal Article Cancer Res · September 1, 2005 Intratumoral infusion is the most commonly used method for viral gene delivery in clinical trials for cancer treatment. However, a potential problem in this approach is that viral vectors may disseminate from tumor to normal tissues during and after the in ... Full text Link to item Cite

Characterisation of systemic dissemination of nonreplicating adenoviral vectors from tumours in local gene delivery.

Journal Article Br J Cancer · April 25, 2005 Systemic virus dissemination is a potential problem during local gene delivery in solid tumours. However, the kinetics and pathways of the dissemination have not been well characterised during the first 24 h after the infusion is started. To this end, we i ... Full text Link to item Cite

Targeting tumor microvessels using doxorubicin encapsulated in a novel thermosensitive liposome.

Journal Article Mol Cancer Ther · October 2004 Liposomal drugs accumulate only in perivascular regions in tumors after i.v. injection. Thus, they cannot kill tumor cells in deeper tissue layers. To circumvent this problem, we investigated effects of doxorubicin (DOX) encapsulated in a lysolecithin-cont ... Link to item Cite

Electric fields within cells as a function of membrane resistivity--a model study.

Journal Article IEEE transactions on nanobioscience · September 2004 Externally applied electric fields play an important role in many therapeutic modalities, but the fields they produce inside cells remain largely unknown. This study makes use of a three-dimensional model to determine the electric field that exists in the ... Full text Cite

Controlled release of ethacrynic acid from poly(lactide-co-glycolide) films for glaucoma treatment.

Journal Article Biomaterials · August 2004 Ethacrynic acid (ECA) is a potential glaucoma drug that can reduce intraocular pressure. However, conventional methods of ECA administration may cause toxicity to normal eye tissues and are inconvenient to patients. Therefore, we developed and characterize ... Full text Link to item Cite

Effects of pulse strength and pulse duration on in vitro DNA electromobility.

Journal Article Bioelectrochemistry (Amsterdam, Netherlands) · April 2004 Interstitial transport of DNA is a rate-limiting step in electric field-mediated gene delivery in vivo. Interstitial transport of macromolecules, such as plasmid DNA, over a distance of several cell layers, is inefficient due to small diffusion coefficient ... Full text Cite

Systemic virus dissemination during local gene delivery in solid tumors and its control with an alginate solution.

Journal Article Conf Proc IEEE Eng Med Biol Soc · 2004 Intratumoral infusion, a routine method for local gene delivery in solid tumors, may cause a systemic dissemination of gene vectors. This is because tumor vessels are intrinsically leaky and intratumoral injection can also result in damage of tumor vessels ... Full text Link to item Cite

Systemic dissemination of viral vectors during intratumoral injection.

Journal Article Mol Cancer Ther · November 2003 Intratumoral injection is a routine method for local viral gene delivery that may improve interstitial transport of viral vectors in tumor tissues and reduce systemic toxicity. However, the concentration of transgene products in normal organs, such as in t ... Link to item Cite

Progress report of a Phase I study of the intracerebral microinfusion of a recombinant chimeric protein composed of transforming growth factor (TGF)-alpha and a mutated form of the Pseudomonas exotoxin termed PE-38 (TP-38) for the treatment of malignant brain tumors.

Journal Article J Neurooncol · October 2003 TP-38 is a recombinant chimeric targeted toxin composed of the EGFR binding ligand TGF-alpha and a genetically engineered form of the Pseudomonas exotoxin, PE-38. After in vitro and in vivo animal studies that showed specific activity and defined the maxim ... Full text Link to item Cite

Reduction of wound angiogenesis in patients treated with BMS-275291, a broad spectrum matrix metalloproteinase inhibitor.

Journal Article Clin Cancer Res · February 2003 PURPOSE: The purpose of this study was to evaluate the feasibility of incorporating a novel wound angiogenesis assay into a Phase I study of BMS-275291, a broad-spectrum matrix metalloproteinase inhibitor, and to determine whether the wound angiogenesis as ... Link to item Cite

Effects of cell damage and glycosaminoglycan degradation on available extravascular space of different dextrans in a rat fibrosarcoma.

Journal Article Int J Hyperthermia · 2003 Drug delivery to solid tumors may be enhanced through increasing the available volume fraction (K(AV)) of drugs. Therefore, two approaches were investigated that may increase K(AV) of dextrans in a rat fibrosarcoma: (a) damaging cells in tumours via ex viv ... Full text Link to item Cite

A clinical model of dermal wound angiogenesis.

Journal Article Wound Repair Regen · 2003 Full-thickness dermal biopsies were performed in healthy volunteers to establish the range of angiogenic responses in wound healing in a normal population. Four-millimeter punch biopsies were made in the forearms of 15 healthy volunteers. Each wound was ev ... Full text Link to item Cite

Reducing systemic toxicity in local tumor gene therapy using an alginate solution

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 2002 To reduce dissemination of viral vectors and their gene products during and after local gene therapy, we mixed adenoviral vectors with an alginate solution and subsequently injected the mixture into mouse tumors. We found that the alginate solution signifi ... Cite

Effects of tissue stretching or cell shrinkage on penetration depth of macromolecules in a rat fibrosarcoma

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 2002 Interstitial penetration is critical for drug delivery in tumor tissues. To experimentally determine the penetration depth of macromolecules at the steady state, rat fibrosarcoma tissues were sectioned into 600μm slices and incubated in solutions of dextra ... Cite

Transscleral diffusion of ethacrynic acid and sodium fluorescein

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 2002 Transscleral drug delivery is an effective method for treatment of eye diseases in posterior segment. Therefore, we investigated transscleral permeability of ethacrynic acid (ECA), a potential drug for treating glaucoma. To determine the ex vivo permeabili ... Cite

Effects of electric pulse strength and pulse duration on plasmid DNA electromobility

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 2002 Interstitial transport of DNA is a rate-limiting step in electric field-mediated gene delivery. Interstitial transport of macromolecules such as plasmid DNA is limited by small diffusion coefficient, large diffusion distance and inadequate convection. Here ... Cite

Electromobility of plasmid DNA in tumor tissues during electric field-mediated gene delivery.

Journal Article Gene Ther · October 2002 Interstitial transport is a crucial step in plasmid DNA-based gene therapy. However, interstitial diffusion of large nucleic acids is prohibitively slow. Therefore, we proposed to facilitate interstitial transport of DNA via pulsed electric fields. To test ... Full text Link to item Cite

An equivalent length model of microdialysis sampling.

Journal Article Journal of pharmaceutical and biomedical analysis · April 2002 One of the critical issues in microdialysis sampling is how to predict the extraction fraction (E(d)), based on transport properties of analytes in both tissues and probes. A one-dimensional (1-D) model has been used widely in previous studies to predict E ... Full text Cite

Intravital fluorescence facilitates measurement of multiple physiologic functions and gene expression in tumors of live animals.

Journal Article Dis Markers · 2002 The purpose of this report is to present an overview of the use of fluorescence imaging in vivo, with particular emphasis on oncology. It is important to note, however, that many of the methods described herein have been applied to the study of non-maligna ... Full text Link to item Cite

Available space and extracellular transport of macromolecules: effects of pore size and connectedness.

Journal Article Annals of biomedical engineering · December 2001 Molecular exclusion in tumor tissues is one of the limiting factors for drug delivery to tumor cells. It can be quantified by the available volume fraction of solutes (K(AV)). We found in a previous study that K(AV) of dextran in tumor tissues decreased sh ... Full text Cite

Quantitative analysis of intratumoral infusion of color molecules.

Journal Article American journal of physiology. Heart and circulatory physiology · August 2001 Intratumoral infusion has a potential for improving distribution of drugs. To optimize the infusion, we developed a novel technique to quantify the distribution volume of color molecules (Vd) in solid tumors. Evans blue-labeled albumin was infused locally ... Full text Cite

Effective tumor therapy with plasmid-encoded cytokines combined with in vivo electroporation.

Journal Article Cancer Res · April 15, 2001 Plasmids may have unique advantages as a gene delivery system. However, a major obstacle is the low in vivo transduction efficiency. In this study, an electroporation-based gene transduction approach was taken to study the effect of interleukin (IL)-2 or I ... Link to item Cite

Numerical simulations of angiogenesis in the cornea.

Journal Article Microvascular research · January 2001 Angiogenesis plays important roles in many physiologic and pathologic processes in the body. To understand mechanisms of angiogenesis, we developed a mathematical model for quantitative analysis of various biological events involved in angiogenesis. Our mo ... Full text Cite

Interstitial hydraulic conductivity in a fibrosarcoma.

Journal Article Am J Physiol Heart Circ Physiol · December 2000 Convective transport of therapeutic agents in solid tumors can be improved through intratumoral infusion. To optimize the convection, we investigated the dependence of the hydraulic conductivity on tissue deformation induced by interstitial fluid pressure ... Full text Link to item Cite

Mobility of plasmid DNA subject to pulsed electric fields

Journal Article Annals of Biomedical Engineering · December 1, 2000 The mobility of plasmid DNA in high amplitude/low duration electric fields was assessed. The influences of pulse amplitude, pulse duration and agarose gel concentration on mobility were compared. Movement of plasmid DNA in an electroporation setting was in ... Cite

Delivery of plasmid DNA through intratumoral infusion and electroporation

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · December 1, 2000 We investigated DNA transport in the interstitial space and across cell membrane facilitated by intratumoral infusion and in vivo electroporation, respectively. In the study, a rat fibrosarcoma was perfused ex vivo, and apparent hydraulic conductivity (Kap ... Cite

New technique for measurement of distribution volume of color molecules in solid tumors

Journal Article Annals of Biomedical Engineering · December 1, 2000 To optimize intramural infusion of macromolecules, a novel technique was developed to quantify the distribution volume of color molecules in solid tumors. It was shown that infusion pressure and volume significantly affects the distribution volume of drugs ... Cite

Available volume fraction of macromolecules in solid tumors

Journal Article Annals of Biomedical Engineering · December 1, 2000 An ex vivo method was developed to quantify available volume fraction (Kav) in tumor tissues and polymer gels. It was shown that Kav is an important transport parameter that depends on physichochemical properties of drugs and tissue structures. ... Cite

Numerical simulations of angiogenesis in the cornea

Journal Article Annals of Biomedical Engineering · December 1, 2000 To understand the mechanisms of angiogenesis, a mathematical model was developed for quantitative analysis of various biological events involved in angiogenesis. The model was focused on twodimensional angiogenesis in the cornea. The model considered diffu ... Cite

Vascular permeability in a human tumour xenograft: molecular charge dependence.

Journal Article British journal of cancer · May 2000 Molecular charge is one of the main determinants of transvascular transport. There are, however, no data available on the effect of molecular charge on microvascular permeability of macromolecules in solid tumours. To this end, we measured tumour microvasc ... Full text Cite

Numerical simulation of angiogenesis in the cornea

Journal Article FASEB JOURNAL · March 15, 2000 Link to item Cite

Delivery of plasmid DNA through intratumoral infusion and electroporation

Conference ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE) · January 1, 2000 We investigated DNA transport in the interstitial spaceand across cell membrane facilitated by intratumoral infusionand in vivo electroporation, respectively. In the study, a ratfibrosarcoma was perfused ex vivo, and apparent hydraulicconductivity (Kaap) w ... Full text Cite

Numerical simulations of angiogenesis in the cornea

Journal Article Annals of Biomedical Engineering · 2000 To understand the mechanisms of angiogenesis, a mathematical model was developed for quantitative analysis of various biological events involved in angiogenesis. The model was focused on twodimensional angiogenesis in the cornea. The model considered diffu ... Cite

Mobility of plasmid DNA subject to pulsed electric fields

Journal Article Annals of Biomedical Engineering · 2000 The mobility of plasmid DNA in high amplitude/low duration electric fields was assessed. The influences of pulse amplitude, pulse duration and agarose gel concentration on mobility were compared. Movement of plasmid DNA in an electroporation setting was in ... Cite

New technique for measurement of distribution volume of color molecules in solid tumors

Journal Article Annals of Biomedical Engineering · 2000 To optimize intramural infusion of macromolecules, a novel technique was developed to quantify the distribution volume of color molecules in solid tumors. It was shown that infusion pressure and volume significantly affects the distribution volume of drugs ... Cite

Pressure and temperature-dependence of the hydraulic conductivity in a fibrosarcoma

Conference Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 1999 Intratumor infusion has been used to deliver therapeutic agents to tumor cells. The distribution volume of infused agents depends on the hydraulic conductivity (K) in tumors, which may vary with the infusion pressure. Therefore, we quantified K in a rat fi ... Cite

Available volume fraction of macromolecules in the extravascular space of a fibrosarcoma: implications for drug delivery.

Journal Article Cancer Res · August 15, 1999 Steric exclusion of molecules in the extravascular space of tissues can be quantified by the available volume fraction (K(AV)). Despite its clinical importance, however, there is a paucity of data in the literature regarding the available volume fraction o ... Link to item Cite

Augmentation of transvascular transport of macromolecules and nanoparticles in tumors using vascular endothelial growth factor.

Journal Article Cancer research · August 1999 The goal of this investigation was to measure changes in vascular permeability, pore cutoff size, and number of transvascular transport pathways as a function of time and in response to vascular endothelial growth factor (VEGF), placenta growth factor (PIG ... Cite

Endothelial cell death, angiogenesis, and microvascular function after castration in an androgen-dependent tumor: role of vascular endothelial growth factor.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · September 1998 The sequence of events that leads to tumor vessel regression and the functional characteristics of these vessels during hormone-ablation therapy are not known. This is because of the lack of an appropriate animal model and monitoring technology. By using i ... Full text Cite

Transvascular drug delivery in solid tumors.

Journal Article Seminars in radiation oncology · July 1998 The microvessel wall is a barrier for the delivery of various therapeutic agents to tumor cells. Tumor microvessels are, in general, more permeable to macromolecules than normal vessels. The hyperpermeability is presumably due to the existence of large por ... Full text Cite

Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · April 1998 Novel anti-neoplastic agents such as gene targeting vectors and encapsulated carriers are quite large (approximately 100-300 nm in diameter). An understanding of the functional size and physiological regulation of transvascular pathways is necessary to opt ... Full text Cite

Modulation of intratumoral drug delivery by the tumor microenvironment and vascular endothelial growth factor

Journal Article Proceedings of the Controlled Release Society · January 1, 1998 Cite

Quantitative angiogenesis assays: progress and problems.

Journal Article Nature medicine · November 1997 Full text Cite

Interlaboratory variation in oxygen tension measurement by Eppendorf "Histograph" and comparison with hypoxic marker.

Journal Article J Surg Oncol · September 1997 BACKGROUND AND OBJECTIVES: The median of pO2 values in tumor measured by Eppendorf "Histograph" with a needle-type electrode has been used as a prognostic indicator in cancer patients. However, it is not established that a pretreatment measured pO2 value c ... Full text Link to item Cite

Effect of host microenvironment on the microcirculation of human colon adenocarcinoma.

Journal Article The American journal of pathology · September 1997 It is generally accepted that the host microenvironment influences tumor biology. There are discrepancies in growth rate, metastatic potential, and efficacy of systemic treatment between ectopic and orthotopic tumors. Liver is the most common and critical ... Cite

Stress is good and bad for tumors.

Journal Article Nature biotechnology · August 1997 Full text Cite

Direct in vivo measurement of targeted binding in a human tumor xenograft.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · March 1997 Binding is crucial to the function of most biologically active molecules, but difficult to quantify directly in living tissue. To this end, fluorescence recovery after photobleaching was used to detect the immobilization of fluorescently labeled ligand cau ... Full text Cite

Role of nitric oxide in tumor microcirculation. Blood flow, vascular permeability, and leukocyte-endothelial interactions.

Journal Article The American journal of pathology · February 1997 The present study was designed to define the role of nitric oxide (NO) in tumor microcirculation, through the direct intravital microcirculatory observations after administration of NO synthase (NOS) inhibitor and NO donor both regionally and systemically. ... Cite

Interstitial pH and pO2 gradients in solid tumors in vivo: high-resolution measurements reveal a lack of correlation.

Journal Article Nature medicine · February 1997 The partial pressure of oxygen (pO2) and pH play critical roles in tumor biology and therapy. We report here the first combined, high-resolution (< or = 10 microns) measurements of interstitial pH and pO2 profiles between adjacent vessels in a human tumor ... Full text Cite

Effect of basic fibroblast growth factor on angiogenesis and growth of isografted bone: quantitative in vitro-in vivo analysis in mice.

Journal Article International journal of microcirculation, clinical and experimental · January 1997 Basic fibroblast growth factor (bFGF), a constituent of bone and cartilage matrix, has been shown to be a potent mitogen for osteoblasts and chondrocytes and yet an inhibitor of chondrocyte terminal differentiation in cell culture. To characterize the effe ... Full text Cite

Time-dependent vascular regression and permeability changes in established human tumor xenografts induced by an anti-vascular endothelial growth factor/vascular permeability factor antibody.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · December 1996 The hyperpermeability of tumor vessels to macromolecules, compared with normal vessels, is presumably due to vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) released by neoplastic and/or host cells. In addition, VEGF/VPF is a pot ... Full text Cite

Perfusion of single tumor microvessels: application to vascular permeability measurement.

Journal Article Microcirculation (New York, N.Y. : 1994) · December 1996 ObjectiveTo develop a new method for determining the relative importance of convection versus diffusion in macromolecular transport across tumor microvessel walls.MethodsThe human colon adenocarcinoma LS174T was transplanted in the dorsal ... Full text Cite

Fluorescence ratio imaging of interstitial pH in solid tumours: effect of glucose on spatial and temporal gradients.

Journal Article British journal of cancer · October 1996 Tumour pH plays a significant role in cancer treatment. However, because of the limitations of the current measurement techniques, spatially and temporally resolved pH data, obtained non-invasively in solid tumours, are not available. Fluorescence ratio im ... Full text Cite

Heating or freezing bone. Effects on angiogenesis induction and growth potential in mice.

Journal Article Acta orthopaedica Scandinavica · August 1996 We have characterized the effect of bone graft treatment by heating or freezing (with or without dimethyl sulfoxide (DMSO)). Tissue culture and dorsal skin-fold chambers in mice were used as sites to quantify the effect on angiogenesis, growth and calcific ... Full text Cite

Quantitation and physiological characterization of angiogenic vessels in mice: effect of basic fibroblast growth factor, vascular endothelial growth factor/vascular permeability factor, and host microenvironment.

Journal Article The American journal of pathology · July 1996 A prerequisite for the development of novel angiogenic and anti-angiogenic agents is the availability of routine in vivo assays that permit 1) repeated, long-term quantitation of angiogenesis and 2) physiological characterization of angiogenic vessels. We ... Cite

Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin.

Journal Article Blood · November 1995 P-selectin-mediated rolling is believed to be important in the recruitment of leukocytes to tissue after ischemia-reperfusion injury. The dorsal skin chamber was used to examine differences in the rolling and stable adhesion of circulating leukocytes in su ... Cite

Vascular permeability in a human tumor xenograft: molecular size dependence and cutoff size.

Journal Article Cancer research · September 1995 Molecular size is one of the key determinants of transvascular transport of therapeutic agents in tumors. However, there are no data in the literature on the molecular size dependence of microvascular permeability in tumors. Therefore, we measured microvas ... Cite

Quantitative analysis of angiogenesis and growth of bone: effect of indomethacin exposure in a combined in vitro-in vivo approach.

Journal Article Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie · January 1995 Nonsteroidal anti-inflammatory agents have been used experimentally and clinically to suppress a variety of physiological events, including angiogenesis and formation of bone. The exact mechanisms by which indomethacin alters skeletal tissue generation are ... Full text Cite

Flow velocity in the superficial lymphatic network of the mouse tail.

Journal Article The American journal of physiology · October 1994 The present study had two goals: 1) to establish an animal model in which a large network of the initial lymphatics of the skin can be investigated in vivo and 2) to measure effective flow velocity (defined as axial component of the flow velocity) in the l ... Full text Cite

Vascular permeability and microcirculation of gliomas and mammary carcinomas transplanted in rat and mouse cranial windows.

Journal Article Cancer research · September 1994 Many brain tumors are highly resistant to chemotherapy, presumably due to the presence of a tight blood-tumor barrier. For a better understanding of the regulation of this barrier by the brain environment, a new intravital microscopy model was established ... Cite

Angiogenesis and growth of isografted bone: quantitative in vivo assay in nude mice.

Journal Article Laboratory investigation; a journal of technical methods and pathology · August 1994 BackgroundUnderstanding the regulation of vascularization and formation of bone after skeletal trauma is essential for the development of methods to promote healing. The lack of information on the biology of bone healing led us to establish an exp ... Cite

Microvascular permeability and interstitial penetration of sterically stabilized (stealth) liposomes in a human tumor xenograft.

Journal Article Cancer research · July 1994 Microvascular permeability and interstitial penetration of sterically stabilized liposomes in both normal s.c. tissue and human colon adenocarcinoma LS174T xenograft were quantified by using the dorsal skin-fold chamber implanted in severe combined immunod ... Cite

Noninvasive measurement of microvascular and interstitial oxygen profiles in a human tumor in SCID mice.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · March 1994 Simultaneous measurements of intravascular and interstitial oxygen partial pressure (PO2) in any tissue have not previously been reported, despite the importance of oxygen in health and in disease. This is due to the limitations of current techniques, both ... Full text Cite

Fluorescence photobleaching with spatial Fourier analysis: measurement of diffusion in light-scattering media.

Journal Article Biophysical journal · December 1993 A new method for the measurement of diffusion in thick samples is introduced, based upon the spatial Fourier analysis of Tsay and Jacobson (Biophys. J. 60: 360-368, 1991) for the video image analysis of fluorescence recovery after photobleaching (FRAP). In ... Full text Cite

Microvascular permeability of albumin, vascular surface area, and vascular volume measured in human adenocarcinoma LS174T using dorsal chamber in SCID mice.

Journal Article Microvascular research · May 1993 A novel method was developed to measure the effective permeability of microvessels in three-dimensional tumors. Two unique features characterized our approach: (i) Texas Red (with peak excitation and peak emission wavelengths of 596 and 615 nm, respectivel ... Full text Cite

Transport of fluid and macromolecules in tumors

Conference American Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD · December 1, 1992 We have characterized steps in drug delivery using two tumor models: (i) an `isolated' tumor which grows in the body with only one arterial input and one venous output, so that we can measure directly what is going into a tumor and what is leaving a tumor; ... Cite

Angiogenesis, microvascular architecture, microhemodynamics, and interstitial fluid pressure during early growth of human adenocarcinoma LS174T in SCID mice.

Journal Article Cancer research · December 1992 To date, most quantitative information on tumor angiogenesis, microcirculation, and transport has been derived from rodent tumors grown in transparent chamber preparations. In this paper we present a chamber technique adapted to immunodeficient mice for th ... Cite

Pharmacokinetic analysis of the perivascular distribution of bifunctional antibodies and haptens: comparison with experimental data.

Journal Article Cancer research · October 1992 A mathematical model is developed to describe the concentration profiles around individual tumor blood vessels for two-step approaches to cancer treatment. The model incorporates plasma pharmacokinetics, interstitial diffusion, reversible binding between a ... Cite

A new view of convective-diffusive transport processes in the arterial intima.

Journal Article Journal of biomechanical engineering · August 1991 In this paper a new theoretical framework is presented for analyzing the filtration and macromolecular convective-diffusive transport processes in the intimal region of an artery wall with widely dispersed macromolecular cellular leakage sites, as proposed ... Full text Cite

Pharmacokinetic analysis of two-step approaches using bifunctional and enzyme-conjugated antibodies.

Journal Article Cancer research · June 1991 Bifunctional antibodies (BFA) and enzyme-conjugated antibodies (ECA) can be used to preferentially deliver a hapten or drug to tumor sites for diagnosis and therapy. We present here a simple pharmacokinetic model for the above two systems by considering on ... Cite

A mathematical model for the receptor mediated cellular regulation of the low density lipoprotein metabolism.

Journal Article Journal of biomechanical engineering · February 1991 A prototype mathematical model for Brown and Goldstein's pioneering studies on the LDL receptor mediated pathway for the regulation of the cellular content of cholesterol has been developed in this paper. In order to analyze the essential features of this ... Full text Cite

New view of convective-diffusive processes in the arterial intima

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · December 1, 1990 Macromolecular transport across the arterial wall is closely linked to a variety of processes in the intima that are believed to play an important role in the subendothelial accumulation of lipid and the formation of the early foam cell lesion. In this pap ... Cite

The displacement wave theory of blood vessel

Journal Article Applied Mathematics and Mechanics · June 1989 Full text Cite