Scholars@Duke
Bruce Klitzman

Bruce Klitzman

Associate Professor Emeritus in Surgery
Surgery, Plastic, Maxillofacial, and Oral Surgery
klitz@duke.edu
Plastic Surgery, Duke University Medical Center, Durham, NC 27710
0112 Baker House, Trent Dr, Durham, NC 27710

Selected Publications

Optimization of the IntraVascular Oxygenator Catheter Using Angular Oscillation.

Journal Article Ann Biomed Eng · March 2024 We demonstrate a methodology which both improves oxygen transport and reduces or eliminates bubble formation in a novel hyperbaric membrane oxygenator catheter model system. Angular oscillations were introduced to a bundle of hollow fiber membranes (HFMs) ... Full text Link to item Cite

Intravascular Gas Exchange: Physiology, Literature Review, and Current Efforts.

Journal Article Respir Care · April 2022 Acute respiratory failure with inadequate oxygenation and/or ventilation is a common reason for ICU admission in children and adults. Despite the morbidity and mortality associated with acute respiratory failure, few proven treatment options exist beyond i ... Full text Link to item Cite

The Influence of Topical Vasodilator-Induced Pharmacologic Delay on Cutaneous Flap Viability and Vascular Remodeling.

Journal Article Plast Reconstr Surg · March 1, 2022 BACKGROUND: Surgical delay is a well-described technique to improve survival of random and pedicled cutaneous flaps. The aim of this study was to test the topical agents minoxidil and iloprost as agents of pharmacologic delay to induce vascular remodeling ... Full text Link to item Cite

Poly(lactide-co-ε-caprolactone) scaffold promotes equivalent tissue integration and supports skin grafts compared to a predicate collagen scaffold.

Journal Article Wound Repair Regen · November 2021 Dermal scarring from motor vehicle accidents, severe burns, military blasts, etc. is a major problem affecting over 80 million people worldwide annually, many of whom suffer from debilitating hypertrophic scar contractures. These stiff, shrunken scars limi ... Full text Link to item Cite

Development of a novel intravascular oxygenator catheter: Oxygen mass transfer properties across nonporous hollow fiber membranes.

Journal Article Biotechnol Bioeng · January 2021 Despite hypoxic respiratory failure representing a large portion of total hospitalizations and healthcare spending worldwide, therapeutic options beyond mechanical ventilation are limited. We demonstrate the technical feasibility of providing oxygen to a b ... Full text Link to item Cite

Ultrasonic bubble detection and tracking using spatial coherence and motion modeling

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2021 Limited options exist to improve oxygenation in patients with acute hypoxic respiratory failure. We are developing an intravascular oxygenator catheter capable of delivering a clinically significant amount of oxygen directly into the bloodstream of patient ... Full text Cite

Detection of flap tissue ischemia in a rat model: Real-time monitoring of changes in oxygenation and perfusion through injectable biosensors.

Journal Article Surgery · November 2020 BACKGROUND: The success of surgical flaps is improved by timely correction of vascular compromise. Current monitoring methods are labor or cost intensive or have limited clinical benefit. We hypothesize that injectable oxygen sensors can identify acute vas ... Full text Link to item Cite

Continuous monitoring of interstitial tissue oxygen using subcutaneous oxygen microsensors: In vivo characterization in healthy volunteers.

Journal Article Microvasc Res · July 2019 Measurements of regional tissue oxygen serve as a proxy to monitor local perfusion and have the potential to guide therapeutic decisions in multiple clinical disciplines. Transcutaneous oximetry (tcpO2) is a commercially available noninvasive technique tha ... Full text Link to item Cite

Machine Perfusion of Liver Grafts With Implantable Oxygen Biosensors: Proof of Concept Study in a Rodent Model.

Journal Article Transplant Direct · July 2019 BACKGROUND: Normothermic machine perfusion (NMP) is emerging as a novel preservation strategy in liver transplantation, but the optimal methods for assessing liver grafts during this period have not been determined. The aim of this study was to investigate ... Full text Link to item Cite

Implantable "smart tattoo" SERS nanosensors for in vivo detection of nucleic acid biotargets in a large animal model

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2019 Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo molecular detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid target ... Full text Cite

Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid targets in a large animal model

Journal Article Nano Research · August 1, 2018 Featured Publication Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid targets using a ... Full text Open Access Cite

Enhanced Drug Delivery to the Skin Using Liposomes.

Journal Article Plast Reconstr Surg Glob Open · July 2018 BACKGROUND: Enhancing drug delivery to the skin has importance in many therapeutic strategies. In particular, the outcome in vascularized composite allotransplantation mainly depends on systemic immunosuppression to prevent and treat episodes of transplant ... Full text Open Access Link to item Cite

Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut.

Journal Article Elife · June 19, 2018 Featured Publication How host and microbial factors combine to structure gut microbial communities remains incompletely understood. Redox potential is an important environmental feature affected by both host and microbial actions. We assessed how antibiotics, which can impact ... Full text Open Access Link to item Cite

Characterization of the Foreign Body Response to Common Surgical Biomaterials in a Murine Model.

Journal Article Eur J Plast Surg · November 2017 Featured Publication BACKGROUND: Implanted biomaterials are subject to a significant reaction from the host, known as the foreign body response (FBR). We quantified the FBR to five materials following subcutaneous implantation in mice. MATERIALS AND METHODS: Polyvinyl alcohol ... Full text Open Access Link to item Cite

Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia.

Journal Article Nature Scientific Reports · August 15, 2017 Novel injectable biosensors were used to measure interstitial oxygenation before, during, and after transient ischemia. It is well known that reactive hyperemia occurs following a period of ischemia. However, increased blood flow does not necessarily mean ... Full text Open Access Link to item Cite

Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia.

Journal Article Sci Rep · August 15, 2017 Featured Publication Novel injectable biosensors were used to measure interstitial oxygenation before, during, and after transient ischemia. It is well known that reactive hyperemia occurs following a period of ischemia. However, increased blood flow does not necessarily mean ... Full text Open Access Link to item Cite

Stiffness of Protease Sensitive and Cell Adhesive PEG Hydrogels Promotes Neovascularization In Vivo.

Journal Article Ann Biomed Eng · June 2017 Featured Publication Materials that support the assembly of new vasculature are critical for regenerative medicine. Controlling the scaffold's mechanical properties may help to optimize neovascularization within implanted biomaterials. However, reducing the stiffness of synthe ... Full text Open Access Link to item Cite

Tissue-Integrating Oxygen Sensors: Continuous Tracking of Tissue Hypoxia.

Journal Article Adv Exp Med Biol · 2017 We describe a simple method of tracking oxygen in real-time with injectable, tissue-integrating microsensors. The sensors are small (500 μm × 500 μm × 5 mm), soft, flexible, tissue-like, biocompatible hydrogel s that have been shown to overcome the foreign ... Full text Open Access Link to item Cite

Monitoring apoptosis and anticancer drug activity in single cells using nanosensors

Chapter · January 1, 2017 One of the key challenges in biology and medicine has been molecular analysis of single living cells aimed at providing an understanding of the underlying complex mechanisms in the basic, structural, and functional unit of life. This is important in unders ... Full text Cite

Plasmonic Gold-Nanostars for Tracking of Adult Stem Cells and for Selective Photothermal Ablation

Conference Journal of the American College of Surgeons · October 2016 Full text Cite

548 Real-time continuous monitoring of subcutaneous tissue oxygenation

Conference Journal of Investigative Dermatology · September 2016 Full text Cite

FOREIGN BODY REACTION TO COMMONLY USED SURGICAL BIOMATERIALS

Journal Article WOUND REPAIR AND REGENERATION · March 1, 2016 Link to item Cite

NOVEL IMPLANTABLE OPTICAL OXYGEN MONITOR TO DETECT FLAP VIABILITY

Journal Article WOUND REPAIR AND REGENERATION · March 1, 2016 Link to item Cite

A medical-toxicological view of tattooing.

Journal Article Lancet · January 23, 2016 Featured Publication Long perceived as a form of exotic self-expression in some social fringe groups, tattoos have left their maverick image behind and become mainstream, particularly for young people. Historically, tattoo-related health and safety regulations have focused on ... Full text Link to item Cite

Porous, Dexamethasone-loaded polyurethane coatings extend performance window of implantable glucose sensors in vivo.

Journal Article Acta Biomater · January 2016 Featured Publication UNLABELLED: Continuous glucose sensors offer the promise of tight glycemic control for insulin dependent diabetics; however, utilization of such systems has been hindered by issues of tissue compatibility. Here we report on the in vivo performance of impla ... Full text Link to item Cite

In vivo detection of SERS-encoded plasmonic nanostars in human skin grafts and live animal models.

Journal Article Anal Bioanal Chem · November 2015 Featured Publication Surface-enhanced Raman scattering (SERS)-active plasmonic nanomaterials have become a promising agent for molecular imaging and multiplex detection. Among the wide variety of plasmonics-active nanoparticles, gold nanostars offer unique plasmon properties t ... Full text Link to item Cite

Improving the Biocompatibility of Implants in Irradiated Tissue Using an Adipose-Derived Stromal-Vascular Fraction

Conference International Journal of Radiation Oncology*Biology*Physics · November 2015 Full text Cite

Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.

Journal Article Sci Rep · October 12, 2015 Featured Publication Tissue-engineered blood vessels (TEBV) can serve as vascular grafts and may also play an important role in the development of organs-on-a-chip. Most TEBV construction involves scaffolding with biomaterials such as collagen gel or electrospun fibrous mesh. ... Full text Open Access Link to item Cite

Novel implantable optical oxygen monitor to detect flap viability

Conference Journal of the American College of Surgeons · October 2015 Full text Cite

Vascularization of Porous PolyHEMA Scaffolds

Conference FASEB JOURNAL · April 1, 2015 Link to item Cite

NOVEL IMPLANTABLE OPTICAL OXYGEN MONITOR TO DETECT FLAP VIABILITY

Conference WOUND REPAIR AND REGENERATION · March 1, 2015 Link to item Cite

FOREIGN BODY RESPONSE TO FIVE COMMON SUBCUTANEOUS IMPLANTS

Conference WOUND REPAIR AND REGENERATION · March 1, 2015 Link to item Cite

Bioburden after Staphylococcus aureus inoculation in type 1 diabetic rats undergoing internal fixation.

Journal Article Plast Reconstr Surg · September 2014 Fracture stabilization in the diabetic patient is associated with higher complication rates, particularly infection and impaired wound healing, which can lead to major tissue damage, osteomyelitis, and higher amputation rates. With an increasing prevalence ... Full text Open Access Link to item Cite

Tissue integration of porous polyHEMA scaffold

Conference FASEB JOURNAL · April 1, 2014 Link to item Cite

A novel immune competent murine hypertrophic scar contracture model: a tool to elucidate disease mechanism and develop new therapies.

Journal Article Wound Repair Regen · 2014 Hypertrophic scar (HSc) contraction following burn injury causes contractures. Contractures are painful and disfiguring. Current therapies are marginally effective. To study pathogenesis and develop new therapies, a murine model is needed. We have created ... Full text Link to item Cite

Micovascular integration into porous polyHEMA scaffold

Chapter · January 1, 2014 Surface-enhanced Raman scattering (SERS) spectroscopy can be a useful tool in regard to disease diagnosis and prevention. Advantage of SERS over conventional Raman spectroscopy is its significantly increased signal (up to factor of 106-108) which allows de ... Full text Cite

Anti-hypotensive treatment and endothelin blockade synergistically antagonize exercise fatigue in rats under simulated high altitude.

Journal Article PLoS One · 2014 Rapid ascent to high altitude causes illness and fatigue, and there is a demand for effective acute treatments to alleviate such effects. We hypothesized that increased oxygen delivery to the tissue using a combination of a hypertensive agent and an endoth ... Full text Open Access Link to item Cite

The effect of nitric oxide surface flux on the foreign body response to subcutaneous implants.

Journal Article Biomaterials · September 2012 Although the release of nitric oxide (NO) from biomaterials has been shown to reduce the foreign body response (FBR), the optimal NO release kinetics and doses remain unknown. Herein, polyurethane-coated wire substrates with varying NO release properties w ... Full text Open Access Link to item Cite

Patient-derived endothelial progenitor cells improve vascular graft patency in a rodent model.

Journal Article Acta Biomater · January 2012 Late outgrowth endothelial progenitor cells (EPCs) derived from the peripheral blood of patients with significant coronary artery disease were sodded into the lumens of small diameter expanded polytetrafluoroethylene (ePTFE) vascular grafts. Grafts (1mm in ... Full text Open Access Link to item Cite

Tissue engraftment of hypoxic-preconditioned adipose-derived stem cells improves flap viability.

Journal Article Wound Repair Regen · 2012 Adipose-derived stem cells (ASCs) have the ability to release multiple growth factors in response to hypoxia. In this study, we investigated the potential of ASCs to prevent tissue ischemia. We found conditioned media from hypoxic ASCs had increased levels ... Full text Open Access Link to item Cite

Implant healing in experimental animal models of diabetes.

Journal Article J Diabetes Sci Technol · May 1, 2011 Diabetes mellitus is becoming increasingly prevalent worldwide. Additionally, there is an increasing number of patients receiving implantable devices such as glucose sensors and orthopedic implants. Thus, it is likely that the number of diabetic patients r ... Full text Open Access Link to item Cite

Increased in vivo glucose recovery via nitric oxide release.

Journal Article Anal Chem · February 15, 2011 The in vivo glucose recovery of subcutaneously implanted nitric oxide (NO)-releasing microdialysis probes was evaluated in a rat model using saturated NO solutions to steadily release NO. Such methodology resulted in a constant NO flux of 162 pmol cm(-2) s ... Full text Open Access Link to item Cite

In vitro fluid dynamics of the Ahmed glaucoma valve modified with expanded polytetrafluoroethylene.

Journal Article Curr Eye Res · February 2011 PURPOSE: Long-term intraocular pressure reduction by glaucoma drainage devices (GDDs) is often limited by the fibrotic capsule that forms around them. Prior work demonstrates that modifying a GDD with a porous membrane promotes a vascularized and more perm ... Full text Open Access Link to item Cite

Bioluminescence imaging of glucose in tissue surrounding polyurethane and glucose sensor implants.

Journal Article J Diabetes Sci Technol · September 1, 2010 BACKGROUND: The bioluminescence technique was used to quantify the local glucose concentration in the tissue surrounding subcutaneously implanted polyurethane material and surrounding glucose sensors. In addition, some implants were coated with a single la ... Full text Open Access Link to item Cite

A model of sequential heart and composite tissue allotransplant in rats.

Journal Article Plast Reconstr Surg · July 2010 BACKGROUND: Some of the 600,000 patients with solid organ allotransplants need reconstruction with a composite tissue allotransplant, such as the hand, abdominal wall, or face. The aim of this study was to develop a rat model for assessing the effects of a ... Full text Open Access Link to item Cite

Intravital microscopy evaluation of angiogenesis and its effects on glucose sensor performance.

Journal Article J Biomed Mater Res A · June 15, 2010 An optical window model for the rodent dorsum was used to perform chronic and quantitative intravital microscopy and laser Doppler flowmetry of microvascular networks adjacent to functional and non-functional glucose sensors. The one-sided configuration af ... Full text Open Access Link to item Cite

Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials.

Journal Article Ann Biomed Eng · June 2010 Resorbable scaffolds such as polyglycolic acid (PGA) are employed in a number of clinical and tissue engineering applications owing to their desirable property of allowing remodeling to form native tissue over time. However, native PGA does not promote end ... Full text Open Access Link to item Cite

Relationship between the canthal-tragus distance and the puncture point in temporomandibular joint arthroscopy.

Journal Article Int J Oral Maxillofac Surg · January 2010 This study investigated whether a relationship exists between the length of the canthal-tragus line and the distance from the tragus at which the puncture point for arthroscope insertion should be made. On one side of 11 cadaver heads, a puncture point was ... Full text Link to item Cite

Expanded polytetrafluoroethylene membrane alters tissue response to implanted Ahmed glaucoma valve.

Journal Article Curr Eye Res · July 2009 PURPOSE: Long-term intraocular pressure control by glaucoma drainage implants is compromised by the formation of an avascular fibrous capsule that surrounds the glaucoma implant and increases aqueous outflow resistance. It is possible to alter this fibroti ... Full text Link to item Cite

Increased signs of acute rejection with ischemic time in a rat musculocutaneous allotransplant model.

Journal Article Transplant Proc · March 2009 BACKGROUND: Composite tissue allotransplantation (CTA) may restore a variety of tissue defects, but carries the potential risks of graft failure and/or immunosuppression-related complications. Ischemia-reperfusion injury has been documented in CTA is known ... Full text Link to item Cite

In vivo cytokine-associated responses to biomaterials.

Journal Article Biomaterials · January 2009 Cytokines, chemokines, and growth factors were analyzed periodically over eight weeks from the wound exudate fluid surrounding biomaterials implanted subcutaneously within stainless steel mesh cages. TNF-alpha, MCP-1, MIP-1alpha, IL-2, IL-6, IL-1beta, VEGF ... Full text Link to item Cite

In vitro and in vivo characterization of porous poly-L-lactic acid coatings for subcutaneously implanted glucose sensors.

Journal Article J Biomed Mater Res A · December 1, 2008 Featured Publication The purpose of this study was to test the hypothesis that porous poly-L-lactic acid (PLLA) sensor coatings reduce fibrosis and promote blood microvessel formation in tissue adjacent to the sensor surface. Porous PLLA coatings were produced using ammonium b ... Full text Link to item Cite

Adipose stem cell attachment to glucose sensors

Conference 8th World Biomaterials Congress 2008 · December 1, 2008 Cite

Percutaneous window chamber method for chronic intravital microscopy of sensor-tissue interactions.

Journal Article J Diabetes Sci Technol · November 2008 BACKGROUND: A dorsal, two-sided skin-fold window chamber model was employed previously by Gough in glucose sensor research to characterize poorly understood physiological factors affecting sensor performance. We have extended this work by developing a perc ... Full text Link to item Cite

IFATS collection: Adipose-derived stromal cells improve the foreign body response.

Journal Article Stem Cells · October 2008 Featured Publication Many implanted devices fail due to the formation of an avascular capsule surrounding the device. Additionally, fat has long been known to promote healing and vascularization. The goals of this study were to identify potential mechanisms of the provascular ... Full text Link to item Cite

Metabolic and functional characterization of human adipose-derived stem cells in tissue engineering.

Journal Article Plast Reconstr Surg · September 2008 BACKGROUND: The use of adipose-derived stem cells for tissue engineering involves exposing them to metabolically adverse conditions. This study examines the metabolism, proliferation, and differentiation of adipose-derived stem cells under various conditio ... Full text Link to item Cite

Advantages of minimally invasive, balloon-dissector-assisted tissue expander placement: a clinical and experimental study.

Journal Article Ann Plast Surg · January 2008 Featured Publication BACKGROUND: Tissue expansion has been dependent upon healing the access incision after placement. Delay can be reduced with minimally placed expanders. Smaller, remote incisions allow for earlier expansion. METHODS: Balloon-assisted tissue expander placeme ... Full text Link to item Cite

Reduced foreign body response at nitric oxide-releasing subcutaneous implants.

Journal Article Biomaterials · November 2007 Featured Publication The tissue response to nitric oxide (NO)-releasing subcutaneous implants is presented. Model implants were created by coating silicone elastomer with diazeniumdiolate-modified xerogel polymers capable of releasing NO. The host tissue response to such impla ... Full text Link to item Cite

Non muscle myosin II regulates contractile phenotypes

Conference Journal of the American College of Surgeons · September 2007 Full text Cite

Efficacy of ethyl nitrite in reversing surgical vasospasm.

Journal Article J Reconstr Microsurg · July 2007 Featured Publication Vasospasm leading to thrombosis is a major cause of free flap failure. Flap blood flow may improve by application of vasodilating agents to the pedicle. The purpose of this study is to evaluate the effect of a novel S-nitrosylating agent, O-ethyl nitrite, ... Full text Link to item Cite

Vascular endothelial growth factor and dexamethasone release from nonfouling sensor coatings affect the foreign body response.

Journal Article J Biomed Mater Res A · June 15, 2007 Featured Publication Vascular endothelial growth factor (VEGF) and dexamethasone (DX) release from hydrogel coatings were examined as a means to modify tissue inflammation and induce angiogenesis. Antibiofouling hydrogels for implantable glucose sensor coatings were prepared f ... Full text Link to item Cite

Combined bone allograft and adipose-derived stem cell autograft in a rabbit model.

Journal Article Ann Plast Surg · May 2007 Featured Publication Currently available options for the repair of bony defects have substantial limitations. Much work has looked to the possibility of engineering bone using stem cells. These tissue-engineering efforts have focused on calvarial defect models, which have the ... Full text Link to item Cite

Adult adipose-derived stem cell attachment to biomaterials.

Journal Article Biomaterials · February 2007 Featured Publication Attachment of adipose-derived stem cells (ASCs) to biomaterials prior to implantation is a possible strategy for mediating inflammation and wound healing. In this study, the ASC percent coverage was measured on common medical grade biosensor materials subj ... Full text Link to item Cite

Vein graft anastomoses with magnets.

Journal Article J Plast Reconstr Aesthet Surg · 2007 Featured Publication BACKGROUND: Performing vascular anastomoses requires complete circumferential access and is time-consuming and technically challenging. The purpose of our study was to assess a sutureless magnetic device for anastomosing vein grafts. METHODS AND RESULTS: O ... Full text Link to item Cite

Direct demonstration of instabilities in oxygen concentrations within the extravascular compartment of an experimental tumor.

Journal Article Cancer Res · February 15, 2006 Featured Publication To test the hypothesis that temporal variations in microvessel red cell flux cause unstable oxygen levels in tumor interstitium, extravascular oxygenation of R3230Ac mammary tumors grown in skin-fold window chambers was measured using recessed tip polarogr ... Full text Link to item Cite

The effects of ZD6474, an inhibitor of VEGF signaling, on cutaneous wound healing in mice.

Journal Article J Surg Res · December 2005 Featured Publication BACKGROUND: ZD6474 is an inhibitor of the VEGFR-2 receptor tyrosine kinase with additional activity against EGFR-1 receptor tyrosine kinases that has been shown to inhibit tumor growth and wound-induced neovascularization in pre-clinical studies and phase ... Full text Link to item Cite

Inhibition of implant-associated infections via nitric oxide release.

Journal Article Biomaterials · December 2005 Featured Publication The in vivo antibacterial activity of nitric oxide (NO)-releasing xerogel coatings was evaluated against an aggressive subcutaneous Staphylococcus aureus infection in a rat model. The NO-releasing implants were created by coating a medical-grade silicone e ... Full text Link to item Cite

Halofuginone inhibits collagen deposition in fibrous capsules around implants.

Journal Article Ann Plast Surg · March 2005 Fibrous capsule formation around implants remains a difficult problem that has been studied for decades. The etiology is elusive, but the end result is the deposition of a dense collagenous capsule around implanted materials. The purpose of this study was ... Link to item Cite

In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts.

Journal Article J Biomed Mater Res B Appl Biomater · January 15, 2005 Featured Publication In this study, we examined combinations of three approaches to improve the adhesion of endothelial cells (EC) onto expanded polytetrafluoroethylene (ePTFE) vascular grafts placed at the femoral artery of rats: (1) high-affinity receptor-ligand binding of R ... Full text Link to item Cite

Method for improved accuracy in endogenous urea recovery marker calibrations for microdialysis in tumors.

Journal Article J Pharmacol Toxicol Methods · 2005 Featured Publication INTRODUCTION: Urea has been proposed as an endogenous recovery marker for microdialysis for absolute concentration calculations of analytes in microdialysis samples. Previously we demonstrated a linear relationship between urea concentrations in a rat mamm ... Full text Link to item Cite

Outflow facility of a Glaucoma Valve enclosed in expanded polytetrafluoroethylene (ePTFE)

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · January 1, 2005 Link to item Cite

Tempostatin™ (halofuginone) inhibits collagen deposition in fibrous capsules

Other Transactions - 7th World Biomaterials Congress · December 1, 2004 A study proposed to see the effectiveness of a collagen type I synthesis inhibitor, Tempostatin™ (Halofuginone), at inhibiting collagen deposition around implanted materials was discussed. The data indicated that halofuginone was effective at inhibiting th ... Cite

Engineered zinc finger-activating vascular endothelial growth factor transcription factor plasmid DNA induces therapeutic angiogenesis in rabbits with hindlimb ischemia.

Journal Article Circulation · October 19, 2004 Featured Publication BACKGROUND: Therapeutic angiogenesis seeks to promote blood vessel growth to improve tissue perfusion. Vascular endothelial growth factor (VEGF) exists in multiple isoforms. We investigated an engineered zinc finger-containing transcription factor plasmid ... Full text Link to item Cite

Immunohistochemical identification of vascular endothelial growth factor in pig latissimus dorsi musculocutaneous flaps following ischemia-reperfusion injury.

Journal Article Ann Plast Surg · October 2004 Featured Publication Vascular Endothelial Growth Factor (VEGF), a potent angiogenic, mitogenic and vascular permeability enhancing protein, appears to improve survival of ischemic flaps independent of its route of administration. The purpose of this study was to examine VEGF p ... Full text Link to item Cite

Side-to-side sutureless vascular anastomosis with magnets.

Journal Article J Vasc Surg · September 2004 Featured Publication OBJECTIVE: Abbe and Payr introduced vascular techniques and devices to facilitate vessel anastomosis over a century ago. Obora published the idea of a sutureless vascular anastomosis with use of magnetic rings in 1978. The purpose of this study was to asse ... Full text Link to item Cite

Anastomoses for vascular shunt, arterial graft, and flap vascularization performed using magnets

Conference JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS · September 1, 2004 Link to item Cite

Basic fibroblast growth factor expression following surgical delay of rat transverse rectus abdominis myocutaneous flaps.

Journal Article Plast Reconstr Surg · June 2004 Featured Publication Partial transverse rectus abdominis myocutaneous (TRAM) flap loss in breast reconstruction can be a devastating complication for both patient and surgeon. Surgical delay of the TRAM flap has been shown to improve flap viability and has been advocated in "h ... Full text Link to item Cite

Vascular endothelial growth factor expression in pig latissimus dorsi myocutaneous flaps after ischemia reperfusion injury.

Journal Article Plast Reconstr Surg · February 2003 Featured Publication Exogenous administration of vascular endothelial growth factor (VEGF) improves long-term viability of myocutaneous flaps. However, endogenous expression of this substance in flaps following ischemia-reperfusion injury has not been reported previously. Endo ... Full text Link to item Cite

Reduction of wound angiogenesis in patients treated with BMS-275291, a broad spectrum matrix metalloproteinase inhibitor.

Journal Article Clin Cancer Res · February 2003 PURPOSE: The purpose of this study was to evaluate the feasibility of incorporating a novel wound angiogenesis assay into a Phase I study of BMS-275291, a broad-spectrum matrix metalloproteinase inhibitor, and to determine whether the wound angiogenesis as ... Link to item Cite

A clinical model of dermal wound angiogenesis.

Journal Article Wound Repair Regen · 2003 Full-thickness dermal biopsies were performed in healthy volunteers to establish the range of angiogenic responses in wound healing in a normal population. Four-millimeter punch biopsies were made in the forearms of 15 healthy volunteers. Each wound was ev ... Full text Link to item Cite

Magnetic resonance imaging of a tissue/implanted device biointerface using in vivo microdialysis sampling.

Journal Article Anal Chem · September 15, 2002 Real-time in vivo images of magnetic resonance contrast agent diffusion from implanted microdialysis probes were obtained by magnetic resonance (MR) microscopy. A gadolinium-containing contrast agent (Gd-DTPA) was infused through microdialysis probes impla ... Full text Link to item Cite

Decreased analyte transport through implanted membranes: differentiation of biofouling from tissue effects.

Journal Article J Biomed Mater Res · December 15, 2001 Membrane biofouling and tissue changes in the foreign body response are known to cause detrimental reductions of analyte transport into implanted biosensors. The relative contribution of each phenomenon is unknown. Hollow fiber microdialysis probes were em ... Full text Link to item Cite

Urea as a recovery marker for quantitative assessment of tumor interstitial solutes with microdialysis.

Journal Article Cancer Res · November 1, 2001 Microdialysis is a technique that enables measurement of extracellular concentrations of unbound analytes. A small probe with a semipermeable membrane is implanted in tissue and constantly perfused. Small analytes in the interstitial fluid diffuse into the ... Link to item Cite

Laser Doppler flowmeter monitoring of free-tissue transfers: blood flow in normal and complicated cases.

Journal Article Plast Reconstr Surg · June 2001 In this article, laser Doppler flowmeter (LDF) monitoring of blood flow in 94 free flaps is summarized. Seventy-six patients had uneventful postoperative courses, and 18 patients developed postoperative complications, with a salvage rate of 88 percent. Exc ... Full text Link to item Cite

Long- and short-term effects of biological hydrogels on capsule microvascular density around implants in rats.

Journal Article J Biomed Mater Res · May 1, 2001 Fibrous capsule formation around implants can inhibit solute exchange between implantable devices and the circulation. Parylene-n coated polycarbonate disks surrounded with growth factor reduced Matrigel (MG) or several gelatin-based matrices were implante ... Full text Link to item Cite

Metabolites at a biomaterial-tissue interface sampled by microdialysis - a comparison of the subcutaneous foreign body response in humans and rats over 8 days

Other Annals of Biomedical Engineering · December 1, 2000 The purpose of this work was to study how closely the rat models the human subcutaneous (SQ) foreign body response. Polyamide microdialysis probes (CMA/Microdialysis; Solna, Sweden) were implanted subcutaneously in healthy human volunteers and in Sprague D ... Cite

Review of methods used to study oxygen transport at the microcirculatory level.

Journal Article Int J Cancer · October 20, 2000 The existence of hypoxic regions in tumors has long been recognized as a key factor leading to radiation resistance. More recently, it has been found that low oxygen levels also affect drug resistance, angiogenesis, cytokine production, cell cycle control, ... Link to item Cite

The role of allopurinol and deferoxamine in preventing pressure ulcers in pigs.

Journal Article Plast Reconstr Surg · April 2000 Ischemia and reperfusion may be important in the pathogenesis of pressure ulcers. On the basis of this hypothesis, the effects of intermittent pressure and the anti-free radical agents allopurinol and deferoxamine were studied in a pig model in which a pre ... Full text Link to item Cite

The effect of muscle flap transposition to the fracture site on TNFalpha levels during fracture healing.

Journal Article Plast Reconstr Surg · March 2000 The trauma and sepsis that follow open fractures and wounds may lead to the production of various cytokines. Understanding wound healing requires a direct knowledge of the specific cytokines and the respective wound fluid levels that are present at the wou ... Full text Link to item Cite

Metabolites at a biomaterial-tissue interface sampled by microdialysis - a comparison of the subcutaneous foreign body response in humans and rats over 8 days

Other Annals of Biomedical Engineering · 2000 The purpose of this work was to study how closely the rat models the human subcutaneous (SQ) foreign body response. Polyamide microdialysis probes (CMA/Microdialysis; Solna, Sweden) were implanted subcutaneously in healthy human volunteers and in Sprague D ... Cite

Wound-induced angiogenesis: A clinical model

Journal Article ORTHOGONAL POLARIZATION SPECTRAL IMAGING · January 1, 2000 Link to item Cite

Characterization of analyte transport over time through implantable biosensor membranes using microdialysis

Other Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 1999 A microdialysis method for monitoring permeability changes of implanted biosensor membranes was demonstrated in vivo. Glucose diffusion through three different hollow fiber membranes (polyethersulfone (PES), polyacrylonitrile (PAN), polycarbonate (PC)) was ... Cite

Quantification of longitudinal tissue pO2 gradients in window chamber tumours: impact on tumour hypoxia.

Journal Article Br J Cancer · April 1999 We previously reported that the arteriolar input in window chamber tumours is limited in number and is constrained to enter the tumour from one surface, and that the pO2 of tumour arterioles is lower than in comparable arterioles of normal tissues. On aver ... Full text Link to item Cite

Multimodality evaluation of pressure relief surfaces.

Journal Article Plast Reconstr Surg · December 1998 Featured Publication Multimodality evaluation of six different support surfaces was performed measuring interface pressure, transcutaneous oxygen tension, and blood flow in pressure ulcer-prone areas on healthy subjects. Interface pressure was measured with a flexible force-se ... Full text Link to item Cite

Comparison of supine and lateral positioning on various clinically used support surfaces.

Journal Article Ann Plast Surg · November 1998 Featured Publication In this study, interface pressures, transcutaneous oxygen tension (TcPO2), and blood flow were compared with the classic pressure ulcer-prone areas in the supine and lateral position on six different high- and low-air-loss support surfaces. Interface press ... Full text Link to item Cite

Pressure ulcers and pressure relief surfaces.

Journal Article Clin Plast Surg · July 1998 Featured Publication Pressure ulcer incidence has been reduced but not eliminated. A few patients still develop pressure ulcers despite using air-fluidized beds and other specialty beds. In the future, very high-risk patients may possibly be sent to space clinics to recuperate ... Link to item Cite

Improved microsurgical anastomotic patency with low molecular weight heparin.

Journal Article J Reconstr Microsurg · July 1998 Blood flow to a free flap may be impaired by thrombotic occlusion at the anastomosis or by microemboli occluding microvessels. The purpose of this study was to test the hypothesis that unfractionated heparin (UFH) or low molecular weight fractions of hepar ... Full text Link to item Cite

Engineering the tissue which encapsulates subcutaneous implants. II. Plasma-tissue exchange properties.

Journal Article J Biomed Mater Res · June 15, 1998 This study assesses the plasma-tissue exchange characteristics of the capsular tissue that forms around implants and how they are affected by implant porosity. The number of vessels and their permeability to rhodamine were measured by intravascular injecti ... Full text Link to item Cite

Engineering the tissue which encapsulates subcutaneous implants. III. Effective tissue response times.

Journal Article J Biomed Mater Res · June 15, 1998 The results of two previous studies have shown that implant porosity can be used to increase both the measured diffusion coefficients and the vascularity within the tissue encapsulating long-term subcutaneous implants. This study investigates the hypothesi ... Full text Link to item Cite

Heparin bonding increases patency of long microvascular prostheses.

Journal Article Plast Reconstr Surg · January 1998 The high thrombogenicity of synthetic biomaterials has limited their use for reconstructive microsurgery. Prime factors in the thrombogenicity of synthetic materials in contact with blood include gas nuclei at the blood gas interface as well as the inheren ... Full text Link to item Cite

Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies.

Journal Article J Biomater Sci Polym Ed · 1998 Synthetic vascular grafts do not spontaneously endothelialize in humans and require some form of anticoagulation to maintain patency. Preseeding synthetic graft materials such as expanded polytetrafluoroethylene (ePTFE) and polyethylene terephthalate (PET) ... Full text Link to item Cite

Effects of internal pressure on blood flow in pig skin

Other FASEB Journal · 1998 Tissue expanders are inflated until the blanched skin and sluggish capillary refill imply poor perfusion. However, expander pressure and cutaneous blood flow immediately following inflation have not been measured. Thus, the purpose of this study was to ide ... Cite

Membrane biofouling measured by microdialysis

Other Proceedings of the Controlled Release Society · January 1, 1998 Cite

Engineering the tissue which encapsulates subcutaneous implants. I. Diffusion properties.

Journal Article J Biomed Mater Res · December 5, 1997 Featured Publication This report uses normal rat subcutis as a reference point to provide a quantitative analysis of small analyte transport through the tissue which encapsulates implants. Polyvinyl alcohol (PVA) with 60- and 350-micron mean pore size (PVA-60, PVA-350), nonpor ... Full text Link to item Cite

Heparin coating of vascular prostheses reduces thromboemboli.

Journal Article Surgery · November 1997 Featured Publication BACKGROUND: Synthetic conduits made from currently available materials are suboptimal for use in small-diameter vascular reconstruction because of their high surface thrombogenicity, which leads to failure. METHODS: In this study control, heparin-irrigated ... Full text Link to item Cite

Laser Doppler flowmetry: a clinical test of pulpal vitality.

Journal Article Oral Surg Oral Med Oral Pathol Oral Radiol Endod · October 1997 PROBLEM: A rapid, accurate, noninvasive method of determining pulpal blood flow would be helpful in determining pulpal vitality. OBJECTIVE: The purpose of this study was to determine if laser Doppler flowmetry can measure induced changes in pulpal blood fl ... Full text Link to item Cite

Modulation of ultraviolet light-induced epidermal damage: beneficial effects of tocopherol.

Journal Article Plast Reconstr Surg · September 1997 Featured Publication Oxygen free radicals have been shown to result from and mediate deleterious effects of ultraviolet radiation on the skin. The purpose of this study was to determine if topical DL-alpha-tocopherol (vitamin E) could reduce ultraviolet-induced damage to the e ... Full text Link to item Cite

Iloprost in alginate decreases the thrombogenicity of expanded polytetrafluoroethylene.

Journal Article J Reconstr Microsurg · May 1997 Featured Publication Vascular prostheses of small diameter perform poorly in vivo, in part because of the high thrombogenicity of available biomaterials. This study examined the thrombogenicity of expanded polytetrafluoroethylene (ePTFE) vascular graft segments (10 mm long x 4 ... Full text Link to item Cite

Brown recluse spider envenomation: a prospective trial of hyperbaric oxygen therapy.

Journal Article Acad Emerg Med · March 1997 OBJECTIVES: Loxosceles reclusa (brown recluse) spider bites can produce severe skin lesions that may necessitate extensive surgical repair. This study delineated the effects of hyperbaric oxygen (HBO) therapy on these lesions by performing a prospective co ... Full text Link to item Cite

Diffusion properties of tissue which encapsulates subcutaneous implants

Other Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering · January 1, 1997 Results are presented of experiments on the diffusion properties of tissue with encapsulates subcutaneous implants. The first experiment was conducted to measure the effective coefficients through capsules which formed around various substrates implanted i ... Cite

Diffusion properties of capsular tissue

Other Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium · December 1, 1996 The effect of capsule formation around a subcutaneous implant is discussed. Polyvinyl alcohol sponge and stainless steel wire mesh materials were implanted. The results show that the diffusion coefficients of fluorescein through capsular tissue are approxi ... Cite

Continual in vivo assessment of 1 mm diameter vascular prostheses

Other Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium · December 1, 1996 An in vivo model of small diameter vascular grafts is developed to quantitatively described the effects of graft treatments on downstream tissue. The model shows that heparin treated grafts and endothelialized grafts performed better than the untreated ePT ... Cite

Fibronectin enhances cell spreading and retention on ePTFE under shear in vitro

Other Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium · December 1, 1996 The extent of cell spreading and retention on a modified endothelial cell lining made of expanded polytetrafluoroethylene (ePTFE) under various shear stresses in vitro were examined. The ePTFE was denucleated and treated either by rinsing in sterile phosph ... Cite

Pulpal response to cavity preparation with the Er:YAG and Mark III free electron lasers.

Journal Article Oral Surg Oral Med Oral Pathol Oral Radiol Endod · June 1996 OBJECTIVE: The purpose of this investigation was to evaluate the pulp response to class V cavity preparation with the use of the Er:YAG laser and free electron laser. STUDY DESIGN: Class V cavities were prepared in 133 teeth of four beagle dogs by one of t ... Full text Link to item Cite

Microsurgical composite tissue transplantation at difficult recipient sites facilitated by preliminary installation of vein grafts as arteriovenous loops.

Journal Article J Reconstr Microsurg · May 1996 In microvascular surgery when local recipient vessels are inadequate, vein grafting is required. There are several potential inherent disadvantages of immediate vein grafting, including the development of graft thrombosis or leakage, an increased opportuni ... Full text Link to item Cite

Limiting impairment of muscle function following ischemia and reperfusion in rabbits.

Journal Article J Reconstr Microsurg · April 1996 Reperfusion injury is a phenomenon complicating microvascular reconstruction. Extensive investigations of free-radical scavengers exist in the literature. The potential beneficial effects of verapamil, deferoxamine, and dimethylsulfoxide were studied in th ... Full text Link to item Cite

Inhibition of nitric oxide synthase on brain oxygenation in anesthetized rats exposed to hyperbaric oxygen.

Journal Article Undersea Hyperb Med · December 1995 Nitric oxide (NO) production is involved in the development of oxygen toxicity of the central nervous system (CNS) since inhibition of nitric oxide synthase (NOS) significantly protects animals from hyperbaric oxygen (HBO)-mediated convulsions. One potenti ... Link to item Cite

Inhibition of radiation-induced up-regulation of leukocyte adhesion to endothelial cells with the platelet-activating factor inhibitor, BN52021.

Journal Article Int J Radiat Oncol Biol Phys · October 15, 1995 PURPOSE: The inflammatory process is likely involved in normal tissue damage after radiation exposure, yet few studies have directly evaluated the factors that might be involved in the regulation of inflammation after irradiation in vivo. We tested the hyp ... Full text Link to item Cite

Assessments of thrombogenicity by three in vitro techniques. Student Research Award in the Undergraduate, Master Candidate, or Health Science Degree Candidate Category, 21st annual meeting of the Society for Biomaterials, San Francisco, CA, March 18-22, 1995.

Journal Article J Biomed Mater Res · September 1995 This study assessed three in vitro techniques designed to measure the thrombogenicity of vascular grafts. All techniques immersed vascular grafts in rotating blood. In the gravimetric analysis, the weight of adherent thrombi was recorded at 2 min intervals ... Full text Link to item Cite

Nitric oxide synthase inhibition and extracellular glutamate concentration after cerebral ischemia/reperfusion.

Journal Article Stroke · February 1995 BACKGROUND AND PURPOSE: Transient cerebral ischemia in rats results in selective loss of neuronal viability, eg, hippocampal CA1 neurons. The neurochemical variables responsible for this selective vulnerability to ischemia/reperfusion (IR) appear to involv ... Full text Link to item Cite

PULPAL RESPONSE TO CAVITY PREPARATION WITH THE ERYAG LASER

Other JOURNAL OF DENTAL RESEARCH · January 1, 1995 Link to item Cite

Blood-flow mapping of oral tissues by laser Doppler flowmetry.

Journal Article Int J Oral Maxillofac Surg · October 1994 The purpose of this study was to develop a rapid protocol for noninvasive quantification of blood flow at intraoral sites by laser Doppler flowmetry. For each flow measurement, the lowest flow observed over a 30-s period was recorded. Three flow readings a ... Full text Link to item Cite

Evaluation of the thrombogenecity of microvascular prosthesis by in vivo microscopy.

Journal Article J Korean Med Sci · October 1994 Expanded polytetrafluoroethylene(ePTFE) grafts 4mm long and 1mm in diameter were implanted into the iliac artery of 100-150g male rats using standard microvascular technique. Prior to clamp removal, the cremaster muscle was isolated as an island flap based ... Full text Link to item Cite

Differences in leucocyte-endothelium interactions between normal and adenocarcinoma bearing tissues in response to radiation.

Journal Article Br J Cancer · May 1994 Previously, we demonstrated that the interaction between leucocytes and endothelial cells in tumour tissues is greatly diminished compared with normal tissues under several induced inflammatory conditions. Radiation has been reported to cause release of in ... Full text Link to item Cite

Rheology and the regulation of oxygen delivery.

Journal Article Microsurgery · 1994 Featured Publication Full text Link to item Cite

Enhanced cardiac preservation with oxygenated University of Wisconsin solution.

Journal Article J Surg Res · December 1993 The purpose of this study was to determine if oxygen delivery to rabbit cardiac allografts arrested and stored in University of Wisconsin solution (UWS) at 4 degrees C would affect preservation. Nineteen isolated rabbit hearts were rapidly excised and perf ... Full text Link to item Cite

Measurement of material extravasation in microvascular networks using fluorescence video-microscopy.

Journal Article Microvasc Res · September 1993 We have developed a new method using fluorescence videomicroscopy to quantitate the extravasation of intravenously injected materials. This method can measure the relative plasma concentration of, and the vascular permeability to, these materials in microc ... Full text Link to item Cite

Quantification of in vitro endothelial cell adhesion to vascular graft material.

Journal Article J Biomed Mater Res · August 1993 This study tests the hypothesis that denucleating vascular graft material and binding cell adhesion molecules increases endothelial attachment. Removal of gas nuclei (denucleation) increases the available surface area of biomaterials for modification and/o ... Full text Link to item Cite

Tissue oxygenation and blood flow on specialized and conventional hospital beds.

Journal Article Ann Plast Surg · May 1993 Despite the widespread use of specialty bed products, the physiological mechanism of their benefit has not been evaluated. In this study, healthy subjects were used to study transcutaneous oxygen tension (TcPo2) and laser Doppler blood flow in pressure sor ... Full text Link to item Cite

Patency and blood flow in gas denucleated arterial prostheses.

Journal Article J Biomed Mater Res · April 1993 Biomaterials exposed to blood often fail due to thrombosis. Gas nuclei (air) in the material are thrombogenic and a potential cause of failure. The effects of gas nuclei on patency and blood flow were studied in 4 mm diameter arterial grafts (Gore ePTFE; J ... Full text Link to item Cite

EFFECT OF HYPERBARIC-OXYGEN ON MUSCLE OXYGEN-TENSIONS

Conference FASEB JOURNAL · February 19, 1993 Link to item Cite

Analysis of oxygen transport to tumor tissue by microvascular networks.

Journal Article Int J Radiat Oncol Biol Phys · February 15, 1993 We present theoretical simulations of oxygen delivery to tumor tissues by networks of microvessels, based on in vivo observations of vascular geometry and blood flow in the tumor microcirculation. The aim of these studies is to investigate the impact of va ... Full text Link to item Cite

A comparison of tumor and normal tissue microvascular hematocrits and red cell fluxes in a rat window chamber model.

Journal Article Int J Radiat Oncol Biol Phys · January 15, 1993 This laboratory has previously used a window chamber model to measure red blood cell velocity in mammary tumors and normal granulation tissues of the F-344 rat. Because red cell flux and hematocrit more accurately reflect the oxygen carrying potential of b ... Full text Link to item Cite

Reduction in tumor blood flow in skin flap tumor after hydralazine is not due to a vascular steal phenomenon

Journal Article Radiation Oncology Investigations · January 1, 1993 The purpose of this study was to evaluate whether a “vascular steal” mechanism (preferential arteriolar vasodilation in normal vs. tumor tissue concomitant with a reduction in blood pressure) is responsible for tumor blood flow reduction after hydralazine ... Full text Cite

Effects of the calcium channel blocker flunarizine on the hemodynamics and oxygenation of tumor microvasculature.

Journal Article Radiat Res · October 1992 Flunarizine is a diphenylpiperazine calcium entry blocker that has been shown previously to increase tumor blood flow and sensitivity to radiotherapy via reduction in the radiobiologically significant hypoxic fraction. Two mechanisms of action have been pr ... Link to item Cite

Diminished leukocyte-endothelium interaction in tumor microvessels.

Journal Article Cancer Res · August 1, 1992 Leukocyte-endothelium interaction in vivo consists of the rolling of leukocytes along the vascular wall and, under certain conditions, their adherence to endothelial cells. In a rat tumor microcirculation model (mammary adenocarcinoma implanted in rat skin ... Link to item Cite

Effects of bradykinin on the hemodynamics of tumor and granulating normal tissue microvasculature.

Journal Article Radiat Res · June 1992 Bradykinin (BK) is an important endogenous mediator of microvascular flow modulation. Since the structure of the microcirculation is very different in tumor tissues than in normal tissues, bradykinin may elicit different responses in tumors. This study was ... Link to item Cite

Perivascular oxygen tensions in a transplantable mammary tumor growing in a dorsal flap window chamber.

Journal Article Radiat Res · May 1992 Fischer 344 rats with R3230 Ac mammary carcinomas implanted in dorsal flap window chambers served as a model to obtain measurements of perivascular and stromal oxygen tension in normal and tumor tissues using Whalen recessed-tip microelectrodes (3- to 6-mi ... Link to item Cite

Reconstruction of rat femoral veins with microvascular prostheses.

Journal Article Plast Reconstr Surg · March 1991 Synthetic conduits have not been suitable for microvascular reconstruction owing primarily to their high thrombogenicity. Vein replacements are the most vulnerable to thrombosis because of their low shear rates and low pressure. Experimental replacement of ... Full text Link to item Cite

Quantification of gas denucleation and thrombogenicity of vascular grafts.

Journal Article J Biomed Mater Res · March 1991 In vitro methods were developed to measure the air content of vascular graft walls and the thrombogenicity of this air. Gas content (volume %) of expanded polytetrafluoroethylene (ePTFE) grafts from different sources ranged from 75.5 +/- 0.4% to 61.8 +/- 0 ... Full text Link to item Cite

Establishment of normal ranges of laser Doppler blood flow in autologous tissue transplants.

Journal Article Plast Reconstr Surg · February 1991 Over a 3-year period, 136 patients were monitored following free autologous tissue transplantation using a laser Doppler flowmeter linked to a computerized data-acquisition system. This monitoring system has indicated perfusion compromise in free flaps mor ... Full text Link to item Cite

Acute difluoromethylornithine treatment increases skin flap survival in rats.

Journal Article Ann Plast Surg · July 1990 Difluoromethylornithine (DFMO) pretreatment for 7 days improved survival of rat abdominal skin flaps in previous studies. The purpose of this study was to determine if acute administration of DFMO enhances survival. Each rat had a 7 x 7-cm abdominal skin f ... Full text Link to item Cite

Quantification of neutrophil adhesion to skeletal muscle venules following ischemia-reperfusion.

Journal Article J Reconstr Microsurg · July 1990 Ischemia-reperfusion is encountered in a wide variety of surgical situations. The damage resulting from ischemia-reperfusion may be due, in part, to the infiltration and activation of neutrophils into the reperfused tissue. The purpose of the study was to ... Full text Link to item Cite

Laser Doppler blood flow measurements of common cutaneous donor sites for reconstructive surgery.

Journal Article Plast Reconstr Surg · April 1990 The purpose of this study was to evaluate cutaneous blood flow in regions commonly used as donor sites in reconstructive surgery in order to better establish normal flow ranges. Flow was measured with the TSI Laserflo BPM 403 in 27 healthy volunteers and c ... Full text Link to item Cite

Stimulation of DNA synthesis in human epidermis by UVB radiation and its inhibition by difluoromethylornithine.

Journal Article Plast Reconstr Surg · April 1990 The purpose of this study was to determine whether the rate of DNA synthesis in human skin could be increased by UVB radiation and to determine the potential for reversing the stimulatory effects of UVB radiation by alpha-difluoromethylornithine (DFMO). Sp ... Full text Link to item Cite

EFFECTS OF HARD AND RESILIENT DENTURE MATERIALS ON TISSUE PERFUSION

Conference JOURNAL OF DENTAL RESEARCH · March 1, 1990 Link to item Cite

Toxic effects of catecholamines on skin.

Journal Article Plast Reconstr Surg · January 1990 The purpose of this study was to examine the effects of catecholamines on skin necrosis independent of their vasoactive effects. Rat abdominal or human breast skin was excised, pinned flat, and incubated at 37 degrees C for 6 hours in a buffered salt solut ... Full text Link to item Cite

Regional variations of laser doppler blood flow in ischemic skin flaps

Conference Plastic and Reconstructive Surgery · January 1, 1990 Full text Cite

The effect of nicotinamide on microvascular density and thermal injury in rats.

Journal Article J Surg Res · November 1989 The effects of nicotinamide on the microvasculature and wound healing were examined in rats subjected to thermal injury. Rats (250 g) were treated with 50 mg nicotinamide intraperitoneally twice daily for 21 days and then heart and brain biopsies were take ... Full text Link to item Cite

Hydrostatic pressure reduces thrombogenicity of polytetrafluoroethylene vascular grafts.

Journal Article Am J Physiol · October 1989 A prime factor in the thrombogenicity of synthetic materials in contact with blood is the blood-gas interface. Small pockets of gas, known as gas nuclei, are trapped within surface interstices. The resulting blood-gas interface denatures plasma proteins an ... Full text Link to item Cite

Decreased thrombogenicity of vascular prostheses following gas denucleation by hydrostatic pressure.

Journal Article Plast Reconstr Surg · December 1988 The high rate of thrombosis of 1.0-mm polytetrafluoroethylene (PTFE) grafts has limited their use in microvascular surgery. One possible reason for this is the blood-gas interface due to entrapped air in the interstices. The present study examines the effe ... Full text Link to item Cite

Reduced cell death in skin flaps in rats treated with difluoromethylornithine.

Journal Article FASEB J · December 1987 The beneficial effects of alpha-difluoromethylornithine (DFMO) were assessed in a model of peripheral ischemia. DFMO is an irreversible inhibitor of ornithine decarboxylase (ODC), the initial enzyme in the production of polyamines. A 7 x 7 cm rat abdominal ... Full text Link to item Cite

Microvascular determinants of blood flow.

Journal Article J Reconstr Microsurg · October 1987 Full text Link to item Cite

Increased survival of skin flaps by scavengers of superoxide radical.

Journal Article FASEB J · August 1987 Elevation of rat abdominal skin flaps, followed by ligation and division of the left inferior neurovascular pedicle, resulted in only a 40% survival of the area normally perfused by the ligated artery and vein. Superoxide dismutase (SOD) (EC 1.15.1.1) admi ... Full text Link to item Cite

Routine clinical use of laser Doppler flowmeter to monitor free tissue transfer: preliminary results.

Journal Article J Reconstr Microsurg · July 1987 Preliminary results indicate that a new laser Doppler flowmeter is more easily understood by nursing personnel than other laser Doppler units currently available. This monitor has demonstrated its capability in identifying and predicting tissue ischemia be ... Full text Link to item Cite

Rat skin flap necrosis reduced with topical chlorpromazine

Other Surgical Forum · January 1, 1987 Cite

Measurement of erythrocyte velocity by use of a periodic differential detector.

Journal Article Am J Physiol · October 1985 An optical velocimeter employing a linear array of photodiodes has been developed and utilized for measuring erythrocyte velocities in the microcirculation. A magnified image of a microvessel is projected and aligned on a one-dimensional array of photodiod ... Full text Link to item Cite

RELATIVE VELOCITY OF RED-CELLS AND SUB-MICRON PARTICLES IN MUSCLE CAPILLARIES

Other INTERNATIONAL JOURNAL OF MICROCIRCULATION-CLINICAL AND EXPERIMENTAL · January 1, 1984 Link to item Cite

A PERIODIC DIFFERENTIAL DETECTOR FOR MICROVASCULAR VELOCIMETRY

Other INTERNATIONAL JOURNAL OF MICROCIRCULATION-CLINICAL AND EXPERIMENTAL · January 1, 1984 Link to item Cite

Oxygen transport in resting and contracting hamster cremaster muscles: experimental and theoretical microvascular studies.

Journal Article Microvasc Res · January 1983 Intravital microscopy of the superfused cremaster muscle was used to measure the density, diameter, length, hematocrit, red cell velocity, and red cell flux in capillaries of the pentobarbital-anesthetized hamster. Oxygen microelectrodes were used to measu ... Full text Link to item Cite

Augmented tissue oxygen supply during striated muscle contraction in the hamster. Relative contributions of capillary recruitment, functional dilation, and reduced tissue PO2.

Journal Article Circ Res · December 1982 To investigate the relative contributions of alterations in blood flow, capillary density, and tissue PO2 to elevated oxygen delivery in working muscle, we conducted experiments on the suffused hamster cremaster muscle, using in vivo microscopic techniques ... Full text Link to item Cite

Capillary network geometry and red cell distribution in hamster cremaster muscle.

Journal Article Am J Physiol · February 1982 Vascular geometry and red cell distribution were examined in 133 capillaries by means of intravital microscopy in the cremaster muscle of the pentobarbital-anesthetized hamster. Significant correlations (P less than 0.01) were found between diameter and fl ... Full text Link to item Cite

Microvascular hematocrit and red cell flow in resting and contracting striated muscle.

Journal Article Am J Physiol · October 1979 Microvascular hematocrit and its possible relation to oxygen supply were systematically examined. We studied the red cell volume fraction (hematocrit) in arterial blood and in capillaries under a variety of circumstances. Control capillary hematocrit avera ... Full text Link to item Cite

Interrelations between contracting striated muscle and precapillary microvessels.

Journal Article Am J Physiol · November 1978 Arterioles and capillaries in the hamster cremaster muscle were observed during electrical stimulation of striated muscle fibers in order to characterize the microcirculatory basis of functional hyperemia. When contraction was restricted to single muscle f ... Full text Link to item Cite