Scholars@Duke
Ralph Snyderman

Ralph Snyderman

James B. Duke Distinguished Professor of Medicine
Medicine
ralph.snyderman@duke.edu
Box 3059 Med Ctr, Durham, NC 27710
2424 Erwin Road, Suite 1101, Durham, NC 27705

Selected Publications

Risk factors of food insecurity among students at diverse post-secondary education institutions: a cross-sectional examination.

Journal Article J Am Coll Health · March 2025 OBJECTIVE: Identify the prevalence of food insecurity (FI) and compare sociodemographic, mental, physical, behavioral, and environmental risk factors for FI among students at a private university, community college, and historically black college or univer ... Full text Link to item Cite

Adaptation to Telehealth of Personalized Group Visits for Late Stage Diabetic Kidney Disease.

Journal Article Kidney360 · December 1, 2023 KEY POINTS: Improving late stage diabetic kidney disease care requires adapting evidence-based, self-management programs for telehealth delivery. We adapted and pilot-tested a telehealth approach and found it to be feasible. Preliminary data suggested it i ... Full text Link to item Cite

Advancing human health in the decade ahead: pregnancy as a key window for discovery: A Burroughs Wellcome Fund Pregnancy Think Tank.

Journal Article Am J Obstet Gynecol · September 2020 Recent revolutionary advances at the intersection of medicine, omics, data sciences, computing, epidemiology, and related technologies inspire us to ponder their impact on health. Their potential impact is particularly germane to the biology of pregnancy a ... Full text Link to item Cite

In Reply to Goetz.

Journal Article Acad Med · July 2020 Full text Link to item Cite

Compassion and Health Care: A Discussion With the Dalai Lama.

Journal Article Acad Med · August 2019 The calling to be a physician has historically been driven by compassion-that is, the desire to relieve the suffering of others. However, the current health care delivery system in the United States has increasingly limited the ability of physicians to exp ... Full text Link to item Cite

An evaluation of mHealth adoption and health self-management in emerging adulthood.

Journal Article AMIA Annu Symp Proc · 2019 This study offers a description of factors that predict the adoption of mobile health technologies (mHealth) and their application for health self-management in emerging adults. Primary data collection occurred at three diverse postsecondary educational in ... Link to item Cite

Integration of Personalized Health Planning and Shared Medical Appointments for Patients with Type 2 Diabetes Mellitus.

Journal Article South Med J · November 2018 OBJECTIVES: This study describes the feasibility of implementing personalized health planning (PHP) within shared medical appointments (SMAs) for patients with type 2 diabetes mellitus. The PHP-SMA approach was designed to synergize the benefits of SMAs wi ... Full text Link to item Cite

Health Care Reform in the United States.

Journal Article JAMA · November 8, 2016 Full text Link to item Cite

A Chancellor's Tale: Transforming Academic Medicine

Book · November 4, 2016 Featured Publication During his fifteen years as chancellor, Dr. Ralph Snyderman helped create new paradigms for academic medicine while guiding the Duke University Medical Center through periods of great challenge and transformation. Under his leadership, the medical center b ... Cite

Value of Personalized Medicine.

Journal Article JAMA · February 9, 2016 Full text Link to item Cite

Personalized Health Planning in Primary Care Settings.

Journal Article Fed Pract · January 2016 Personalized health planning can be operationalized as a health care delivery model to support personalized, proactive, patient-driven care. ... Open Access Link to item Cite

Patient engagement as a risk factor in personalized health care: A systematic review of the literature on chronic disease

Journal Article Genome Medicine · February 26, 2014 Background: The role of patient engagement as an important risk factor for healthcare outcomes has not been well established. The objective of this article was to systematically review the relationship between patient engagement and health outcomes in chro ... Full text Cite

Patient engagement as a risk factor in personalized health care: a systematic review of the literature on chronic disease.

Journal Article Genome Med · 2014 BACKGROUND: The role of patient engagement as an important risk factor for healthcare outcomes has not been well established. The objective of this article was to systematically review the relationship between patient engagement and health outcomes in chro ... Full text Link to item Cite

Personalized health care in 2013: a status report on the impact of genomics.

Journal Article N C Med J · 2013 This issue of the NCMJ describes the impact that genomics has had on the practice of medicine in the decade since the full sequencing of the human genome was completed in 2003. Specifically, it reports on how genomics is affecting health care delivery, des ... Link to item Cite

Personalized health care as a pathway for the adoption of genomic medicine.

Journal Article J Pers Med · November 13, 2012 While the full promise of genomic medicine may be many years in the future, personalized health care (PHC) can begin solving important health care needs now and provide a framework for the adoption of genomic technologies as they are validated. PHC is a st ... Full text Link to item Cite

Personalized health care: from theory to practice.

Journal Article Biotechnol J · August 2012 The practice of medicine stands at the threshold of a transformation from its current focus on the treatment of disease events to an emphasis on enhancing health, preventing disease and personalizing care to meet each individual's specific health needs. Pe ... Full text Link to item Cite

Personalized medicine is more than genomic medicine: confusion over terminology impedes progress towards personalized healthcare.

Journal Article Per Med · January 2012 Over the last decade, scientific discovery and technological advances have created great anticipation for capabilities to tailor individual medical decisions and provide personalized healthcare. Despite some advances, adoption has been sporadic and there r ... Full text Link to item Cite

Introduction of Robert J. Lefkowitz.

Journal Article J Clin Invest · October 2011 Full text Link to item Cite

The Role of Genomics in Enabling Prospective Health Care

Chapter · December 1, 2010 Microbiology has a profound impact on concepts concerning the cause and potential cure of illnesses, through the identification of causative agents for numerous infectious diseases. Given the increasing number of diseases determined to have specific causes ... Full text Cite

Commentary: Personalized health planning and the Patient Protection and Affordable Care Act: an opportunity for academic medicine to lead health care reform.

Journal Article Acad Med · November 2010 The Patient Protection and Affordable Care Act of 2010 (PPACA) mandates the exploration of new approaches to coordinated health care delivery--such as patient-centered medical homes, accountable care organizations, and disease management programs--in which ... Full text Link to item Cite

Improving health by taking it personally.

Journal Article JAMA · January 27, 2010 Full text Link to item Cite

Creating meaningful health care reform.

Journal Article J Clin Invest · October 2009 Full text Link to item Cite

More about prospective health care.

Journal Article Acad Med · May 2009 Full text Link to item Cite

IOM publishes agenda for national summit on integrative medicine

Journal Article Integrative Medicine · 2009 Cite

Perspective: Prospective health care and the role of academic medicine: lead, follow, or get out of the way.

Journal Article Acad Med · August 2008 The authors contend that the crisis facing the U.S. health care system is in large part a consequence of that system's disease-oriented, reactive, and sporadic approach to care, and they suggest that a prospective approach to health care, which emphasizes ... Full text Link to item Cite

Proposal for a new health record to support personalized, predictive, preventative and participatory medicine.

Journal Article Per Med · January 2008 Today's approach to patient care and the medical record that directs and documents it is largely focused on identifying and treating the patient's disease. This has resulted in a sporadic, reactive healthcare system. Shifting medicine's focus to personaliz ... Full text Link to item Cite

Meeting the challenges facing clinical research: solutions proposed by leaders of medical specialty and clinical research societies.

Journal Article Acad Med · February 2006 The development of a robust national clinical research enterprise is needed to improve health care, but faces formidable challenges. To define the impediments and formulate solutions, the Institute of Medicine's Clinical Research Roundtable convened leader ... Full text Link to item Cite

Prospective health care: the second transformation of medicine.

Journal Article Genome Biol · 2006 Emerging scientific technologies provide rich sources of predictive biomarkers, which could transform health care. Identification of causal biomarkers will enable the development of tools to quantify risk and anticipate disease. Accurate health risk analys ... Full text Link to item Cite

The debt repayment paradox for VA clinical investigators - Reply

Journal Article JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION · 2004 Full text Cite

Prospective medicine: the next health care transformation.

Journal Article Acad Med · November 2003 The introduction of science into the practice of medicine in the early 20th century was a transforming event for the profession. Now, breakthroughs in science and know how make it possible to transform care once again and to fix the broken U.S. health care ... Full text Link to item Cite

Interleukin-8-mediated heterologous receptor internalization provides resistance to HIV-1 infectivity. Role of signal strength and receptor desensitization.

Journal Article J Biol Chem · May 2, 2003 Human immunodeficiency virus type 1 (HIV-1) entry into CD4(+) cells requires the chemokine receptors CCR5 or CXCR4 as co-fusion receptors. We have previously demonstrated that chemokine receptors are capable of cross-regulating the functions of each other ... Full text Link to item Cite

Personalized health planning.

Journal Article Science · April 25, 2003 Full text Link to item Cite

Role of the cytoplasmic tails of CXCR1 and CXCR2 in mediating leukocyte migration, activation, and regulation.

Journal Article J Immunol · March 15, 2003 IL-8 (or CXCL8) activates the receptors CXCR1 (IL-8RA) and CXCR2 (IL-8RB) to induce chemotaxis in leukocytes, but only CXCR1 mediates cytotoxic and cross-regulatory signals. This may be due to the rapid internalization of CXCR2. To investigate the roles of ... Full text Link to item Cite

Integrative medicine is a trojan horse.

Journal Article Arch Intern Med · November 11, 2002 Full text Link to item Cite

Integrative medicine is a Trojan horse - Reply

Journal Article ARCHIVES OF INTERNAL MEDICINE · November 11, 2002 Link to item Cite

Phospholipase C-beta 2 interacts with mitogen-activated protein kinase kinase 3.

Journal Article Biochem Biophys Res Commun · April 26, 2002 Phospholipase C (PLC)-beta enzymes (isoenzymes beta 1-beta 4) are activated by G protein subunits, leading to the generation of intracellular messengers which mobilize calcium and activate protein kinase C. It has recently been recognized that these enzyme ... Full text Link to item Cite

Integrative medicine: bringing medicine back to its roots.

Journal Article Arch Intern Med · February 25, 2002 Full text Link to item Cite

RGS4 inhibits platelet-activating factor receptor phosphorylation and cellular responses.

Journal Article Biochemistry · March 27, 2001 To define the role of regulators of G-protein signaling (RGS) in chemoattractant-mediated responses, RGS4 and the receptors for platelet-activating factor (PAFR), formylated peptides (FR), or interleukin-8 (CXCR1) were stably coexpressed in a rat basophili ... Full text Link to item Cite

Ralph Snyderman, MD - CAM and the role of the academic health center

Journal Article ALTERNATIVE THERAPIES IN HEALTH AND MEDICINE · September 1, 2000 Link to item Cite

Targeted disruption of the leukotriene B(4) receptor in mice reveals its role in inflammation and platelet-activating factor-induced anaphylaxis.

Journal Article J Exp Med · August 7, 2000 Leukotrienes are derived from arachidonic acid and serve as mediators of inflammation and immediate hypersensitivity. Leukotriene B(4) (LTB(4)) and leukotriene C(4) (LTC(4)) act through G protein-coupled receptors LTB(4) receptor (BLTR) and Cys-LTR, respec ... Full text Link to item Cite

Regulation of the human chemokine receptor CCR1. Cross-regulation by CXCR1 and CXCR2.

Journal Article J Biol Chem · March 31, 2000 To investigate the regulation of the CCR1 chemokine receptor, a rat basophilic leukemia (RBL-2H3) cell line was modified to stably express epitope-tagged receptor. These cells responded to RANTES (regulated upon activation normal T expressed and secreted), ... Full text Link to item Cite

Clinical research.

Journal Article Science · March 17, 2000 Full text Link to item Cite

Identification of a region at the N-terminus of phospholipase C-beta 3 that interacts with G protein beta gamma subunits.

Journal Article Biochemistry · February 22, 2000 Members of the phospholipase C-beta (PLC-beta) family of proteins are activated either by G alpha or G beta gamma subunits of heterotrimeric G proteins. To define specific regions of PLC-beta 3 that are involved in binding and activation by G beta gamma, a ... Full text Link to item Cite

Oversight mechanisms for clinical research.

Journal Article Science · January 28, 2000 Full text Link to item Cite

Function and regulation of chemoattractant receptors.

Journal Article Immunol Res · 2000 Phagocyte migration and activation at sites of inflammation is mediated through chemoattractant receptors that are coupled to G-proteins. Early studies from our laboratory demonstrated G-protein-mediated phospholipase C activation by chemoattractants. Rece ... Full text Link to item Cite

Chemoattractant receptors activate distinct pathways for chemotaxis and secretion. Role of G-protein usage.

Journal Article J Biol Chem · December 24, 1999 Human leukocyte chemoattractant receptors activate chemotactic and cytotoxic pathways to varying degrees and also activate different G-proteins depending on the receptor and the cell-type. To determine the relationship between G-protein usage and the biolo ... Full text Link to item Cite

Chemoattractant receptor cross-desensitization.

Journal Article J Biol Chem · March 5, 1999 Full text Link to item Cite

Differential cross-regulation of the human chemokine receptors CXCR1 and CXCR2. Evidence for time-dependent signal generation.

Journal Article J Biol Chem · September 11, 1998 Neutrophils and transfected RBL-2H3 cells were used to investigate the mechanism of cross-regulation of the human interleukin-8 (IL-8) receptors CXCR1 and CXCR2 by chemoattractants. In neutrophils, Ca2+ mobilization by the CXCR2-specific chemokine, growth- ... Full text Link to item Cite

Opiates transdeactivate chemokine receptors: delta and mu opiate receptor-mediated heterologous desensitization.

Journal Article J Exp Med · July 20, 1998 An intact chemotactic response is vital for leukocyte trafficking and host defense. Opiates are known to exert a number of immunomodulating effects in vitro and in vivo, and we sought to determine whether they were capable of inhibiting chemokine-induced d ... Full text Link to item Cite

Differential regulation of formyl peptide and platelet-activating factor receptors. Role of phospholipase Cbeta3 phosphorylation by protein kinase A.

Journal Article J Biol Chem · May 1, 1998 Formylated peptides (e.g. n-formyl-Met-Leu-Phe (fMLP)) and platelet-activating factor (PAF) mediate chemotactic and cytotoxic responses in leukocytes through receptors coupled to G proteins that activate phospholipase C (PLC). In RBL-2H3 cells, fMLP utiliz ... Full text Link to item Cite

Multiple signaling pathways of human interleukin-8 receptor A. Independent regulation by phosphorylation.

Journal Article J Biol Chem · April 24, 1998 Interleukin-8 (IL-8) receptor A (CXCR1) couples to a pertussis toxin-sensitive G protein to mediate phospholipase Cbeta (PLCbeta) activation and cellular responses. Responses to CXCR1 are attenuated by prior exposure of neutrophils to either IL-8, a cleava ... Full text Link to item Cite

L-selectin-dependent leukocyte rolling on endothelial monolayers under flow

Journal Article FASEB Journal · December 1, 1997 Lcukocyte-endothelial (L/E) interactions during inflammation are regulated by the synchronized binding of endothelial adhesion molecules with corresponding leukocyte counter-receptors, including L-selectin. The dynamic interactions of rat basophilic leukem ... Cite

Regulation of human chemokine receptors CXCR4. Role of phosphorylation in desensitization and internalization.

Journal Article J Biol Chem · November 7, 1997 Members of the chemokine receptor family CCR5 and CXCR4 have recently been shown to be involved in the entry of human immunodeficiency virus (HIV) into target cells. Here, we investigated the regulation of CXCR4 in rat basophilic leukemia cells (RBL-2H3) s ... Full text Link to item Cite

Chemoattractant receptor-induced phosphorylation of L-selectin.

Journal Article J Biol Chem · May 23, 1997 The selectin adhesion molecules and chemoattractant receptors synergistically regulate leukocyte migration into lymphoid tissues and sites of inflammation, but little is known about how these families of receptors modulate each other's function. In this st ... Full text Link to item Cite

Role of phospholipase Cbeta3 phosphorylation in the desensitization of cellular responses to platelet-activating factor.

Journal Article J Biol Chem · May 2, 1997 Platelet-activating factor (PAF) stimulates a diverse array of cellular responses through receptors coupled to G proteins that activate phospholipase C (PLC). Truncation of the cytoplasmic tail of the receptor to remove phosphorylation sites (mutant PAF re ... Full text Link to item Cite

Mechanisms of inflammation and leukocyte activation.

Journal Article Med Clin North Am · January 1997 This article reviews the current status of the knowledge of mechanisms of activating inflammatory responses. It also describes inflammatory mediators, adhesion proteins, the inflammatory process itself, and the molecular mechanisms controlling inflammatory ... Full text Link to item Cite

Advances in rheumatology - Preface

Journal Article MEDICAL CLINICS OF NORTH AMERICA · January 1, 1997 Link to item Cite

Chemoattractant receptor-induced phosphorylation of L-selectin.

Journal Article JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY · January 1, 1997 Link to item Cite

Cross-desensitization among receptors for platelet activating factor and peptide chemoattractants. Evidence for independent regulatory pathways.

Journal Article J Biol Chem · November 8, 1996 Cross-desensitization among receptors for peptide chemoattractants have been shown to involve two independent processes, receptor phosphorylation and inhibition of phospholipase C (PLC) activation. Receptors for lipid chemoattractants, i.e. platelet activa ... Full text Link to item Cite

The Jim interview - Ralph Snyderman, MD

Journal Article JOURNAL OF INVESTIGATIVE MEDICINE · October 1, 1996 Link to item Cite

Thrombin primes responsiveness of selective chemoattractant receptors at a site distal to G protein activation.

Journal Article J Biol Chem · February 9, 1996 To define the molecular basis of human chemoattractant receptor regulation, rat basophilic leukemia RBL-2H3 cells, which are thrombin-responsive, were transfected to stably express epitope-tagged receptors for C5a, interleukin-8 (IL-8), formylpeptides (e.g ... Full text Link to item Cite

Cross-desensitization of chemoattractant receptors occurs at multiple levels. Evidence for a role for inhibition of phospholipase C activity.

Journal Article J Biol Chem · November 17, 1995 To define the molecular mechanisms of cross-regulation among chemoattractant receptors, we stably coexpressed, in a rat basophilic leukemia (RBL-2H3) cell line, epitope-tagged receptors for the chemoattractants formylmethionylleucylphenylalanine (fMLP), a ... Full text Link to item Cite

Regulation of human interleukin-8 receptor A: identification of a phosphorylation site involved in modulating receptor functions.

Journal Article Biochemistry · October 31, 1995 The human type A interleukin-8 receptor (IL-8RA) was modified to express an amino-terminal epitope tag and stably overexpressed in a rat basophilic leukemia cell line (RBL-2H3). This receptor (ET-IL-8RA) displayed functional properties similar to those of ... Full text Link to item Cite

Human calcium-calmodulin dependent protein kinase I: cDNA cloning, domain structure and activation by phosphorylation at threonine-177 by calcium-calmodulin dependent protein kinase I kinase.

Journal Article EMBO J · August 1, 1995 Human Ca(2+)-calmodulin (CaM) dependent protein kinase I (CaMKI) encodes a 370 amino acid protein with a calculated M(r) of 41,337. The 1.5 kb CaMKI mRNA is expressed in many different human tissues and is the product of a single gene located on human chro ... Full text Link to item Cite

Inherited human complement C5 deficiency. Nonsense mutations in exons 1 (Gln1 to Stop) and 36 (Arg1458 to Stop) and compound heterozygosity in three African-American families.

Journal Article J Immunol · May 15, 1995 Hereditary C5 deficiency has been reported in several families of different ethnic backgrounds and from different geographic regions, but the molecular genetic defect causing C5 deficiency has not been delineated in any of them. To examine the molecular ba ... Link to item Cite

Regulation of stably transfected platelet activating factor receptor in RBL-2H3 cells. Role of multiple G proteins and receptor phosphorylation.

Journal Article J Biol Chem · October 7, 1994 Platelet activating factor (PAF) interacts with cell surface receptors to mediate inflammatory responses. To determine the mechanisms of PAF receptor regulation, we constructed epitope-tagged human PAF receptor cDNA (ET-PAFR) and generated stable transfect ... Link to item Cite

Cross-desensitization of receptors for peptide chemoattractants. Characterization of a new form of leukocyte regulation.

Journal Article J Immunol · October 1, 1994 The formylpeptide (fMLP) and C5a chemoattractants were previously shown to cross-desensitize each other's ability to mobilize Ca2+ in leukocytes but not to affect nonchemoattractant Ca(2+)-mobilizing receptors, and vice versa. Our data show that all recept ... Link to item Cite

Role of phosphate-magnesium-binding regions in the high GTPase activity of rac1 protein.

Journal Article Biochemistry · December 7, 1993 rac1, a member of the low molecular mass GTP-binding protein family, has a 20-fold higher GTPase activity than H-ras, but the structural motifs responsible for this property do not appear to reside within the conserved amino acids of the consensus GTP-bind ... Full text Link to item Cite

Differences in phosphorylation of formylpeptide and C5a chemoattractant receptors correlate with differences in desensitization.

Journal Article J Biol Chem · November 15, 1993 To define the regulation of chemoattractant receptors, epitope-tagged human formyl peptide and C5a receptor cDNAs (ET-FR and ET-C5aR) were stably expressed in rat basophilic leukemia, RBL-2H3 cells. An antibody (12CA5) specific to "ET" was used to immunopr ... Link to item Cite

Identification of additional members of human G-protein-coupled receptor kinase multigene family.

Journal Article Proc Natl Acad Sci U S A · October 15, 1993 Human neutrophils express several distinct guanine nucleotide binding (G)-protein-coupled receptors that mediate their responsiveness to chemoattractants. Phosphorylation by receptor-specific and second messenger-activated protein kinases is a common mecha ... Full text Link to item Cite

Rac1, a low-molecular-mass GTP-binding-protein with high intrinsic GTPase activity and distinct biochemical properties.

Journal Article Eur J Biochem · June 1, 1992 Rac1, a member of the family of low-molecular-mass GTP-binding proteins, functions in phagocytic leukocytes as a component necessary for activation of the respiratory burst. To characterize the biochemical properties of rac1, the protein was expressed as a ... Full text Link to item Cite

Differential regulation of cAMP by endogenous versus transfected formylpeptide chemoattractant receptors: implications for Gi-coupled receptor signaling.

Journal Article Biochem Biophys Res Commun · March 31, 1992 Endogenous neutrophil formylpeptide receptors do not inhibit adenylylcyclase activation. The ability of a cloned and transfected human formylpeptide receptor to mediate the inhibition of adenylylcyclase was assessed in the human embryonic kidney 293 TSA ce ... Full text Link to item Cite

Functional high efficiency expression of cloned leukocyte chemoattractant receptor cDNAs.

Journal Article FEBS Lett · February 10, 1992 Human kidney 293 TSA cells were transfected by a calcium phosphate method with human formylpeptide and C5a receptor cDNAs with high efficiency. Formylpeptide receptor positive transfectants expressed a total of 968,000 +/- 34,000 receptors per cell with tw ... Full text Link to item Cite

Both stimulatory and inhibitory GDP/GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins.

Journal Article Biochem Biophys Res Commun · January 31, 1992 Six peaks of small GTP-binding proteins (G proteins) were separated by column chromatographies from the cytosol fraction of the differentiated HL-60 cells: two peaks of rho p21, one peak of smg/rap1 p21, two peaks of rac1 p21, and one peak of an unidentifi ... Full text Link to item Cite

CHEMOATTRACTANT STIMULUS-RESPONSE COUPLING

Conference MONONUCLEAR PHAGOCYTES · January 1, 1992 Link to item Cite

Receptor class desensitization of leukocyte chemoattractant receptors.

Journal Article Proc Natl Acad Sci U S A · December 15, 1991 To better define their regulation, formylpeptide and C5a chemoattractant receptor cDNAs were transiently expressed with high efficiency (approximately 35-54%) in human kidney cells. As in neutrophils, both receptors were active in elevating intracellular c ... Full text Link to item Cite

A synthetic peptide homologous to retroviral transmembrane envelope proteins depresses protein kinase C mediated lymphocyte proliferation and directly inactivated protein kinase C: a potential mechanism for immunosuppression.

Journal Article Microbiol Immunol · 1991 CKS-17, an immunosuppressive peptide homologous to certain retroviral transmembrane envelope protein, has been shown to inhibit lymphocyte proliferation in response to mitogens or alloantigens when covalently attached to bovine serum albumin (CKS-17-BSA). ... Full text Link to item Cite

Molecular mechanisms of leukocyte activation by chemoattractants.

Journal Article Agents Actions Suppl · 1991 Chemoattractant receptors on leukocytes initiate migratory and cytotoxic activities via GTP-binding proteins. Recent cloning of cDNA encoding the formylpeptide receptor indicates it to be a member of the class of seven membrane spanning domain receptors wh ... Link to item Cite

MOLECULAR-BASIS OF LEUKOCYTE CHEMOTACTIC RESPONSES

Conference CELLULAR AND CYTOKINE NETWORKS IN TISSUE IMMUNITY · January 1, 1991 Link to item Cite

Isoprenylation of the low molecular mass GTP-binding proteins rac 1 and rac 2: possible role in membrane localization.

Journal Article Biochem Biophys Res Commun · September 14, 1990 Ras proteins can be modified at their COOH-terminal cysteine in the motif Cys-Ali-Ali-Xaa by a farnesyl isoprenoid. This modification is essential for membrane association and biological activity of ras proteins. A similar COOH-terminal amino acid sequence ... Full text Link to item Cite

A soluble inhibitor of T lymphocyte function induced by HIV-1 infection of CD4+ T cells: characterization of a cellular protein and its relationship to p15E.

Journal Article Cell Immunol · July 1990 Soluble suppressor factor (SSF), first described in association with HIV-1 infection in vivo, is a molecule(s) capable of inhibiting T cell-dependent immune reactivity. Its relationship to human immunodeficiency virus (HIV) was further defined as supernata ... Full text Link to item Cite

A Synthetic Peptide Homologous to Retroviral Transmembrane Envelope Proteins Depresses Protein Kinase C Mediated Lymphocyte Proliferation and Directly Inactivated Protein Kinase C: A Potential Mechanism for Immunosuppression

Journal Article MICROBIOLOGY and IMMUNOLOGY · January 1, 1990 CKS-17, an immunosuppressive peptide homologous to certain retroviral transmembrane envelope protein, has been shown to inhibit lymphocyte proliferation in response to mitogens or alloantigens when covalently attached to bovine serum albumin (CKS-17-BSA). ... Cite

rac, a novel ras-related family of proteins that are botulinum toxin substrates.

Journal Article J Biol Chem · October 5, 1989 A new family of ras-related proteins, designated rac (ras-related C3 botulinum toxin substrate) has been identified. rac1 and rac2 cDNA clones were isolated from a differentiated HL-60 library and encode proteins that are 92% homologous and share 58% and 2 ... Link to item Cite

Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets.

Journal Article J Biol Chem · October 5, 1989 Identification of the GTP-binding proteins from human platelet particulate fractions was attained by their purification via successive column chromatography steps followed by amino acid sequencing. To enhance the likelihood of identifying the GTP-binding p ... Link to item Cite

Mastoparan, a wasp venom peptide, identifies two discrete mechanisms for elevating cytosolic calcium and inositol trisphosphates in human polymorphonuclear leukocytes.

Journal Article J Immunol · September 1, 1989 Mastoparan, a tetradecapeptide toxin from wasp venom stimulates secretion in mast cells and enhances GTPase activity of several purified guanine nucleotide regulatory proteins (G proteins). This suggests that this toxin may effect cellular functions throug ... Link to item Cite

Stimulation of phosphorylcholine turnover and diacylglycerol production in human polymorphonuclear leukocytes. Novel assay for phosphorylcholine.

Journal Article Biochem J · June 15, 1989 Receptor-bypassing stimulants of human polymorphonuclear leukocytes (PMNLs), such as ionomycin or phorbol 12-myristate 13-acetate (PMA), generate an increase in diacylglycerol (DAG) which is independent of a phospholipase C specific for phosphatidylinosito ... Full text Link to item Cite

Substance P primes human neutrophil activation: a mechanism for neurological regulation of inflammation.

Journal Article Biochem Biophys Res Commun · June 15, 1989 The neuropeptide substance P (SP), a member of the tachykinin family, has stimulatory effects on various cell types at nanomolar concentrations. SP has also direct effects on polymorphonuclear leukocytes (PMNs). However, unlike other cells, stimulation of ... Full text Link to item Cite

Molecular cloning of the cDNA for ligatin.

Journal Article J Cell Sci · June 1989 We describe the first isolation and sequence of a partial cDNA clone encoding ligatin, a trafficking receptor for phosphoglycoproteins. The clone was isolated from a human U937 promonocyte lambda gt11 cDNA library using rabbit antiserum to rat ileal ligati ... Full text Link to item Cite

Multiple chromatographic forms of the formylpeptide chemoattractant receptor and their relationship to GTP-binding proteins.

Journal Article Biochem Biophys Res Commun · May 30, 1989 The radiolabeled formylpeptide chemoattractant receptor, partially purified by wheat germ agglutinin-Sepharose chromatography, eluted in three distinct peaks when chromatographed on DEAE-Fractogel. Incubation of the lectin-Sepharose purified receptor with ... Full text Link to item Cite

Characterization of G25K, a GTP-binding protein containing a novel putative nucleotide binding domain.

Journal Article Biochem Biophys Res Commun · April 14, 1989 Amino acid sequences were obtained for four peptides (p1, -2, -3 and 4) generated by chemical or proteolytic cleavage of a 25 kDa GTP-binding protein purified from human placental and platelet membranes. The peptides shared sequence similarities with those ... Full text Link to item Cite

GRAM, A NOVEL RAS-RELATED GENE FAMILY CLONED FROM MYELOID CELLS

Journal Article CLINICAL RESEARCH · April 1, 1989 Link to item Cite

Production of a retroviral P15E-related chemotaxis inhibitor by IL-1-treated endothelial cells. A possible negative feedback in the regulation of the vascular response to monokines.

Journal Article J Immunol · March 15, 1989 The effects of IL-1 on vascular endothelium result in a complex set of alterations which are potentially disruptive of vessel wall and underlying tissue integrity. The present study was aimed at investigating possible regulation of such potentially destruc ... Link to item Cite

Analysis of calcium homeostasis in activated human polymorphonuclear leukocytes. Evidence for two distinct mechanisms for lowering cytosolic calcium.

Journal Article J Biol Chem · January 15, 1989 The stimulation of polymorphonuclear leukocytes (PMNs) by chemoattractants triggers a rapid rise in cytosolic free calcium concentration(s) ([Ca2+]i), which quickly returns to base line, suggesting a role for calcium removal in the homeostasis of activated ... Link to item Cite

Effects of CKS-17, a synthetic retroviral envelope peptide, on cell-mediated immunity in vivo: immunosuppression, immunogenicity, and relation to immunosuppressive tumor products.

Journal Article Cancer Immunol Immunother · 1989 CKS-17 is a heptadecapeptide corresponding to a region highly conserved in retroviral transmembrane proteins such as p15E. Because a relationship had previously been determined between p15E and immunosuppressive tumor cell products, we examined the effect ... Full text Link to item Cite

Modulation of inositol phospholipid metabolism by polyamines.

Journal Article Biochem J · November 15, 1988 At low concentrations of Mg2+, incorporation of 32P from [gamma-32P]ATP into phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) in plasma membranes isolated from human polymorphonuclear leucocytes was enhanced 2-4-fold ... Full text Link to item Cite

Human retrovirus-related synthetic peptides inhibit T lymphocyte proliferation.

Journal Article Immunol Lett · September 1988 Immunosuppression frequently accompanies infections with the human retroviruses HTLV-1 and HIV. Previous studies have shown that UV-inactivated and detergent-disrupted preparations of either virus can produce immune dysfunction in vitro although the active ... Full text Link to item Cite

Human IFN-gamma production is inhibited by a synthetic peptide homologous to retroviral envelope protein.

Journal Article J Immunol · July 15, 1988 A synthetic 17 amino acid peptide (CKS-17) homologous to a highly conserved region of human and animal retroviral transmembrane proteins was investigated for its influence on the in vitro production of IFN-gamma from human peripheral mononuclear cells. The ... Link to item Cite

Retroviral protein P15E and tumorigenesis. Expression is neither required nor sufficient for tumor development.

Journal Article J Immunol · June 1, 1988 Murine tumor cells frequently express retroviral protein p15E, a protein with antiinflammatory activity. This has led to the hypothesis that p15E expression allows nascent tumor cells to escape host immunologic defenses. To evaluate the role of p15E expres ... Link to item Cite

Binding of alpha 2-macroglobulin-thrombin complexes and methylamine-treated alpha 2-macroglobulin to human blood monocytes.

Journal Article Biochemistry · April 19, 1988 The binding of alpha 2-macroglobulin (alpha 2M) to human peripheral blood monocytes was investigated. Monocytes, the precursors of tissue macrophages, were isolated from fresh blood by centrifugal elutriation or density gradient centrifugation. Binding stu ... Full text Link to item Cite

The formylpeptide chemoattractant receptor copurifies with a GTP-binding protein containing a distinct 40-kDa pertussis toxin substrate.

Journal Article J Biol Chem · April 5, 1988 Detergent extraction of plasma membranes from differentiated HL60 cells, specifically labeled with the chemoattractant, formyl-Nle-Leu-Phe-Nle-[125I-Tyr] Lys, resulted in the solubilization of a receptor-radioligand complex. GTP-binding activity coeluted w ... Link to item Cite

Behçet's disease--provocative clues.

Journal Article West J Med · April 1988 Link to item Cite

Behçet's disease--provocative clues.

Journal Article The Western journal of medicine · April 1988 Cite

Calcium influx stimulates a second pathway for sustained diacylglycerol production in leukocytes activated by chemoattractants.

Journal Article Proc Natl Acad Sci U S A · March 1988 Metabolic pathways involved in the activation of polymorphonuclear leukocytes (PMNs) were characterized by using chemoattractants with equivalent chemotactic activity but widely disparate ability to stimulate superoxide production [N-formylmethionylleucylp ... Full text Link to item Cite

Leukocyte chemoattraction by 1,2-diacylglycerol.

Journal Article Proc Natl Acad Sci U S A · March 1988 Previous reports have demonstrated the hydrolysis of inositol phospholipids in polymorphonuclear leukocytes (PMN) in response to chemoattractants and in lymphocytes in response to the mitogen phytohemagglutinin. We investigated the role of 1,2-diacylglycer ... Full text Link to item Cite

Leukocyte chemoattractant receptors.

Journal Article Methods Enzymol · 1988 Full text Link to item Cite

Signal transduction in cells following binding of chemoattractants to membrane receptors.

Journal Article Virchows Arch B Cell Pathol Incl Mol Pathol · 1988 Binding of chemoattractants to specific cell surface receptors on human polymorphonuclear leukocytes (PMNs) initiates a variety of biologic responses, including directed migration (chemotaxis), release of superoxide anions, and lysosomal enzyme secretion. ... Full text Link to item Cite

Regulation of inositol phospholipid and inositol phosphate metabolism in chemoattractant-activated human polymorphonuclear leukocytes.

Journal Article J Cell Biochem · December 1987 Binding of chemoattractants to specific cell surface receptors on polymorphonuclear leukocytes (PMNs) initiates a series of biochemical responses leading to cellular activation. A critical early biochemical event in chemoattractant (CTX) receptor-mediated ... Full text Link to item Cite

IMMUNOLOGY OF THE RHEUMATIC DISEASES - PREFACE

Journal Article RHEUMATIC DISEASE CLINICS OF NORTH AMERICA · December 1, 1987 Link to item Cite

Isolation of GTP-binding proteins from myeloid HL-60 cells. Identification of two pertussis toxin substrates.

Journal Article J Biol Chem · November 15, 1987 We have isolated the major GTP-binding proteins from myeloid HL-60 cell plasma membranes. Two pertussis toxin substrates with similar apparent molecular masses of 40 and 41 kDa, respectively, are contained in these preparations, with both proteins being AD ... Link to item Cite

Recombinant hydrophilic region of murine retroviral protein p15E inhibits stimulated T-lymphocyte proliferation.

Journal Article Proc Natl Acad Sci U S A · October 1987 Retroviral envelope protein p15E and antigenically related proteins have been implicated as potential mediators of immune dysfunction associated with retroviral infections and with neoplasia. Due to its extreme hydrophobicity, purified p15E has not been av ... Full text Link to item Cite

A synthetic peptide homologous to the envelope proteins of retroviruses inhibits monocyte-mediated killing by inactivating interleukin 1.

Journal Article J Immunol · October 1, 1987 The synthetic peptide CKS-17 has homology to a highly conserved region of the immunosuppressive retroviral envelope protein P15E, to envelope proteins of HTLV I, II, III, and to that encoded by an endogeneous C-type human retroviral DNA. CKS-17 inhibits th ... Link to item Cite

Regulation of inositol phosphate metabolism in chemoattractant-stimulated human polymorphonuclear leukocytes. Definition of distinct dephosphorylation pathways for IP3 isomers.

Journal Article J Biol Chem · August 25, 1987 The metabolism of the calcium mobilizing inositol-1,4,5-trisphosphate (IP3) isomer was studied in myo-[3H]inositol labeled, chemoattractant-stimulated human polymorphonuclear neutrophils (PMNs), and in PMN lysates. It was determined that 1,4,5-IP3 is metab ... Link to item Cite

Molecular cloning of a new human G protein. Evidence for two Gi alpha-like protein families.

Journal Article FEBS Lett · July 13, 1987 The amino acid sequence of a novel G protein alpha subunit (Gx alpha) has been deduced from the nucleotide sequence of a human cDNA clone isolated from a differentiated HL-60 cDNA library. The cDNA encodes a polypeptide of 354 amino acids (Mr 40,519) which ... Full text Link to item Cite

Role of a guanine nucleotide regulatory protein in the activation of phospholipase C by different chemoattractants.

Journal Article J Immunol · June 15, 1987 It is well established that formyl peptide chemoattractants can activate a phospholipase C in leukocytes via a pertussis toxin (PT)-sensitive guanine nucleotide regulatory (G) protein. Whether this pathway is similarly used by chemoattractant receptors as ... Link to item Cite

Chemoattractant-induced activation of c-fos gene expression in human monocytes.

Journal Article J Exp Med · June 1, 1987 Human monocytes use the products of phosphoinositide hydrolysis (1,2-diacylglycerol and inositol 1,4,5-triphosphate) as second messengers to trigger rapid cellular activation during the occupancy of chemoattractant receptors. The effect of chemoattractants ... Full text Link to item Cite

Nucleotide regulatory protein-mediated activation of phospholipase C in human polymorphonuclear leukocytes is disrupted by phorbol esters.

Journal Article J Biol Chem · May 5, 1987 Polymorphonuclear leukocytes (PMNs) activate phospholipase C via a guanine nucleotide regulatory (G) protein. Pretreatment of the PMNs with pertussis toxin (PT) or 4-beta-phorbol 12-myristate 13-acetate (PMA) inhibited chemoattractant-induced inositol tris ... Link to item Cite

Identification of a novel inositol bisphosphate isomer formed in chemoattractant stimulated human polymorphonuclear leukocytes.

Journal Article Biochem Biophys Res Commun · April 14, 1987 Analysis of inositol phosphate formation in chemoattractant-stimulated human polymorphonuclear leukocytes demonstrated the production of inositol 1,4,5-trisphosphate, inositol 1,3,4-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, inositol 1,4-bisphospha ... Full text Link to item Cite

A conserved region at the COOH terminus of human immunodeficiency virus gp120 envelope protein contains an immunodominant epitope.

Journal Article Proc Natl Acad Sci U S A · April 1987 A highly immunogenic epitope from a conserved COOH-terminal region of the human immunodeficiency virus (HIV) gp120 envelope protein has been identified with antisera from HIV-seropositive subjects and a synthetic peptide (SP-22) containing 15 amino acids f ... Full text Link to item Cite

Chemotactic factor and P15E-related chemotaxis inhibitor in human melanoma cell lines with different macrophage content and tumorigenicity in nude mice.

Journal Article J Immunol · April 1, 1987 The present study was designed to characterize the production of chemoattractants by human melanoma lines with high (M4Be, M3Da, NTerDa) or low tumorigenic (Doc8, M1Do) potential when heterotransplanted in nude mice. Supernatants from the Doc8 and M1Do cel ... Link to item Cite

Inhibition of human natural killer cell activity by a synthetic peptide homologous to a conserved region in the retroviral protein, p15E.

Journal Article J Immunol · February 1, 1987 It has been shown previously that the retroviral envelope protein p15E suppresses certain monocyte and lymphocyte functions. In this paper, we describe the effects on natural killer (NK) activity of a synthetic peptide (CKS-17) with homology to a region of ... Link to item Cite

Altered cell-averaged microviscosity of murine peritoneal macrophages undergoing activation in vivo or in vitro.

Journal Article Cell Immunol · February 1987 The cell-averaged microviscosity of intact murine peritoneal mononuclear phagocytes in various stages of activation was assessed by quantifying fluorescent depolarization of 1,6-diphenyl-1,3,5-hexatriene. Macrophages activated in vivo with Mycobacterium bo ... Full text Link to item Cite

Human Gi protein alpha-subunit: deduction of amino acid structure from a cloned cDNA.

Journal Article FEBS Lett · January 26, 1987 The amino acid sequence of the alpha-subunit of Gi, the human adenylate cyclase inhibiting GTP-binding protein, has been deduced from the nucleotide sequence of a DNA clone complementary to Gi alpha mRNA from differentiated U937 cells. The cDNA encodes a p ... Full text Link to item Cite

Suppressive effect on polyclonal B-cell activation of a synthetic peptide homologous to a transmembrane component of oncogenic retroviruses.

Journal Article Proc Natl Acad Sci U S A · January 1987 Purified feline leukemia virus, UV light-inactivated feline leukemia virus, and a synthetic peptide (CKS-17) homologous to a well-conserved region of the transmembrane components of several human and animal retroviruses were each studied for their effects ... Full text Link to item Cite

Isolation of GTP-binding proteins from myeloid HL-60 cells. Identification of two pertussis toxin substrates

Journal Article Journal of Biological Chemistry · January 1, 1987 We have isolated the major GTP-binding proteins from myeloid HL-60 cell plasma membranes. Two pertussis toxin substrates with similar apparent molecular masses of 40 and 41 kDa, respectively, are contained in these preparations, with both proteins being AD ... Cite

Leukocyte activation by chemoattractant receptors: roles of a guanine nucleotide regulatory protein and polyphosphoinositide metabolism.

Journal Article Rev Infect Dis · 1987 Chemoattractant receptors on leukocytes are coupled to a guanine nucleotide regulatory (N or G) protein that stimulates a membrane-associated phospholipase C to hydrolyze phosphatidylinositol 4,5-biphosphate. The products of this hydrolysis are inositol 1, ... Full text Link to item Cite

Lymphoma models for B cell activation and tolerance. V. Anti-Ig mediated growth inhibition is reversed by phorbol myristate acetate but does not involve changes in cytosolic free calcium.

Journal Article J Mol Cell Immunol · 1987 B cell lymphomas which can be growth inhibited by crosslinking their surface IgM receptors by anti-Ig reagents provide models for normal B cell regulation and tolerance. WEHI-231 and CH31 are two independently derived lines that are exquisitely sensitive t ... Link to item Cite

Model for leukocyte regulation by chemoattractant receptors: roles of a guanine nucleotide regulatory protein and polyphosphoinositide metabolism.

Journal Article J Leukoc Biol · December 1986 Binding of chemoattractants to their receptors on phagocytes activates a guanine nucleotide regulatory (N) protein through the substitution of GTP for GDP on N. The activated N protein in turn stimulates a membrane-associated phospholipase C by lowering th ... Full text Link to item Cite

Coexistence of a chemotactic factor and a retroviral P15E-related chemotaxis inhibitor in human tumor cell culture supernatants.

Journal Article J Immunol · October 15, 1986 Two sets of seemingly contradictory evidence have been reported concerning the effects of tumor cell products on the regulation of monocyte migration in vitro and presumably the extravasation of macrophages into tumors in vivo. The present study was design ... Link to item Cite

A pertussis/choleratoxin-sensitive N protein may mediate chemoattractant receptor signal transduction.

Journal Article Biochem Biophys Res Commun · July 31, 1986 Chemoattractant receptors on phagocytic leukocytes utilize a guanine nucleotide regulatory (N) protein to activate phospholipase C and subsequent biological responses. Since pertussis toxin inhibits activation of leukocytes by chemoattractants and ribosyla ... Full text Link to item Cite

A guanine nucleotide regulatory protein controls polyphosphoinositide metabolism, Ca2+ mobilization, and cellular responses to chemoattractants in human monocytes.

Journal Article J Immunol · July 1, 1986 Previous studies demonstrated that oligopeptide chemoattractant receptors on PMN and macrophages exist in high and low affinity states which are interconvertible by guanosine di- and triphosphates. These observations suggest that guanine nucleotide regulat ... Link to item Cite

Differential stimulation of the respiratory burst and lysosomal enzyme secretion in human polymorphonuclear leukocytes by synthetic diacylglycerols.

Journal Article J Immunol · June 15, 1986 Binding of chemoattractants to receptors on human polymorphonuclear leukocytes (PMN) stimulates the phosphodiesteric cleavage of phosphatidylinositol 4,5-bisphosphate to produce inositol 1,4,5-trisphosphate and 1,2-diacylglycerols. To investigate the possi ... Link to item Cite

Extensive hydrolysis of N-formyl-L-methionyl-L-leucyl-L-[3H] phenylalanine by human polymorphonuclear leukocytes. A potential mechanism for modulation of the chemoattractant signal.

Journal Article J Biol Chem · April 15, 1986 Chemoattractant receptors on human polymorphonuclear leukocytes (PMNs) stimulate a series of important biological responses. In an attempt to better understand the mechanism of stimulus response coupling of chemoattractant receptors, the kinetics of N-form ... Link to item Cite

Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein.

Journal Article Science · April 4, 1986 Cleavage of phosphatidylinositol 4,5-bisphosphate by phospholipase C results in the production of two important second messengers: inositol-1,4,5-trisphosphate and 1,2-diacylglycerol. Although several receptors promote this cleavage, the molecular details ... Full text Link to item Cite

Chemoattractant-mediated stimulation of the respiratory burst in human polymorphonuclear leukocytes may require appearance of protein kinase activity in the cells' particulate fraction.

Journal Article Blood · April 1986 Low doses of aliphatic alcohols produce divergent effects on the function of chemoattractant receptors on human polymorphonuclear leukocytes (PMNs) since they enhance chemotaxis but inhibit stimulation of superoxide production by chemoattractants. As such, ... Link to item Cite

Mechanisms of inflammation and leukocyte chemotaxis in the rheumatic diseases.

Journal Article Med Clin North Am · March 1986 Understanding the mechanisms of inflammatory cell accumulation, as well as how such cells mediate tissue destruction, provides better insights into the pathogenesis and therapeutics of the rheumatic diseases. This article discusses the role of the inflamma ... Full text Link to item Cite

PNEUMONITIS DURING LOW-DOSE METHOTREXATE THERAPY - REPLY

Journal Article ARCHIVES OF INTERNAL MEDICINE · March 1, 1986 Link to item Cite

Foreword

Journal Article Medical Clinics of North America · March 1986 Full text Cite

In Reply

Journal Article Archives of Internal Medicine · January 1, 1986 Full text Cite

Effects of tumor growth on host defenses.

Journal Article Cancer Metastasis Rev · 1986 Monocytes and macrophages play an important role in host defense against neoplasia. Studies from our and other laboratories have demonstrated that patients with a variety of cancers have a defect in monocyte chemotactic responses. Tumor-bearing mice are al ... Full text Link to item Cite

Role of guanine nucleotide regulatory protein in polyphosphoinositide degradation and activation of phagocytic leukocytes by chemoattractants.

Journal Article J Cell Biochem · 1986 Leukocyte activation by chemoattractants provides an important model to study the biochemical mechanisms of stimulus-response coupling in these cells. Well-defined chemotactic factors induce readily quantifiable responses in phagocytic leukocytes. These in ... Full text Link to item Cite

Pneumonitis complicating low-dose methotrexate therapy in rheumatoid arthritis.

Journal Article Arch Intern Med · November 1985 Three of 95 patients with rheumatoid arthritis who were being treated with low-dose (5 to 15 mg/wk) methotrexate sodium developed the clinical, radiographic, and pathologic features of methotrexate-associated pulmonary injury. Marked hypoxemia emphasized t ... Full text Link to item Cite

Redistribution of protein kinase C activity in human monocytes: correlation with activation of the respiratory burst.

Journal Article J Immunol · November 1985 Protein kinase C (PKC) was found to be present in purified human monocytes and lymphocytes isolated by countercurrent centrifugal elutriation. In unstimulated monocytes and lymphocytes, approximately 90% of the PKC activity was cytosolic when the cells wer ... Link to item Cite

Inhibition of lymphocyte proliferation by a synthetic peptide homologous to retroviral envelope proteins.

Journal Article Science · October 25, 1985 The retroviral transmembrane envelope protein p15E is immunosuppressive in that it inhibits immune responses of lymphocytes, monocytes, and macrophages. A region of p15E has been conserved among murine and feline retroviruses; a homologous region is also f ... Full text Link to item Cite

Phorbol myristate acetate mediates redistribution of protein kinase C in human neutrophils: potential role in the activation of the respiratory burst enzyme.

Journal Article J Immunol · September 1985 Protein kinase C may be important in leukocyte function, because it is activated by phorbol myristate acetate (PMA), a potent stimulus of the respiratory burst in neutrophils. The localization of protein kinase C was compared in unstimulated and PMA-stimul ... Link to item Cite

Successful tumour immunotherapy: possible role of antibodies to anti-inflammatory factors produced by neoplasms.

Journal Article Clin Exp Immunol · July 1985 Phenol-saline tumour extracts, active in the immunotherapy of bovine ocular squamous cell carcinoma (BOSCC), were used to immunize mice. The immunized mice became resistant to the depression of delayed type hypersensitivity (DTH) reactions by products of B ... Link to item Cite

Chemoattractant-elicited alterations of cAMP levels in human polymorphonuclear leukocytes require a Ca2+-dependent mechanism which is independent of transmembrane activation of adenylate cyclase.

Journal Article J Biol Chem · June 10, 1985 The affinity of the chemoattractant receptor for N-formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe) on human polymorphonuclear leukocytes (PMNs) is regulated by guanine nucleotides, and chemoattractants stimulate increased intracellular cAMP levels in ... Link to item Cite

Chemoattractant receptor-induced hydrolysis of phosphatidylinositol 4,5-bisphosphate in human polymorphonuclear leukocyte membranes. Requirement for a guanine nucleotide regulatory protein.

Journal Article J Biol Chem · May 25, 1985 Incubation of plasma membranes from human polymorphonuclear leukocytes (PMNs) with [gamma-32P]ATP in the presence of MgCl2 resulted in the formation of 32P-labeled phosphatidic acid (PA), phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol 4,5 ... Link to item Cite

Activation of the respiratory burst enzyme from human neutrophils in a cell-free system. Evidence for a soluble cofactor.

Journal Article J Clin Invest · May 1985 Activation of the respiratory burst in phagocytic cells, an important host defense process, is not yet well understood. We now report the development of a cell-free system for activation of NADPH oxidase, the respiratory burst enzyme, in human neutrophils. ... Full text Link to item Cite

Inhaled asbestos activates a complement-dependent chemoattractant for macrophages.

Journal Article Lab Invest · May 1985 Pulmonary macrophages migrate to the sites where inhaled chrysotile asbestos fibers initially are deposited (i.e., surfaces of alveolar duct bifurcations). These macrophages have been shown to form a major component of an early asbestos-induced interstitia ... Link to item Cite

Potential role for a guanine nucleotide regulatory protein in chemoattractant receptor mediated polyphosphoinositide metabolism, Ca++ mobilization and cellular responses by leukocytes.

Journal Article Biochem Biophys Res Commun · March 15, 1985 Islet activating protein from Bordetella pertussis toxin which ribosylates certain guanine nucleotide regulatory proteins causes a marked reduction of chemoattractant-elicited responses such as chemotaxis, O2 production and cAMP elevations in human polymor ... Full text Link to item Cite

Enhanced tumorigenicity of transformed NIH/3T3 cells which produce retroviral P15E

Journal Article Proceedings of the American Association for Cancer Research · January 1, 1985 Cite

The phagocytic cell: summary.

Journal Article Rev Infect Dis · 1985 Full text Link to item Cite

Regulatory mechanisms of a chemoattractant receptor on human polymorphonuclear leukocytes.

Journal Article Rev Infect Dis · 1985 The oligopeptide chemotactic factor receptor in leukocyte membranes exists in two affinity states that are in part interconvertible. Convertibility is regulated by guanine nucleotides, which suggests that a nucleotide regulatory unit allosterically modifie ... Full text Link to item Cite

ENHANCED TUMORIGENICITY OF TRANSFORMED NIH/3T3 CELLS WHICH PRODUCE RETROVIRAL P15E

Conference PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH · January 1, 1985 Link to item Cite

Light scattering by polymorphonuclear leukocytes stimulated to aggregate under various pharmacologic conditions.

Journal Article Blood · September 1984 Enhancement of light transmission has been widely accepted as an empirical measure of cell aggregation in suspension. Several years ago, this measurement was introduced to the study of polymorphonuclear leukocyte (PMN) aggregation by adapting a hypothesis ... Link to item Cite

Regulatory mechanisms of a chemoattractant receptor on leukocytes.

Journal Article Fed Proc · September 1984 Chemoattractant receptors on leukocytes initiate a number of coordinated biochemical and biological processes in a strict dose-related manner. Chemotaxis-related functions occur at low doses of chemoattractants whereas the microbicidal or secretory functio ... Link to item Cite

Immunosuppressive activity of the retroviral envelope protein P 15E and its possible relationship to neoplasia.

Journal Article Immunol Today · August 1984 Type C retroviral infections can cause profound immunosuppression as well as neoplasms. The retroviral envelope protein p15E has both immunosuppressive and anti-inflammatory activities which may contribute to the pathogenicity of retroviruses. Murine and h ... Full text Link to item Cite

A potential second messenger role for unsaturated fatty acids: activation of Ca2+-dependent protein kinase.

Journal Article Science · May 11, 1984 Arachidonate and other unsaturated long-chain fatty acids were found to activate protein kinase C from human neutrophils. Kinase activation by arachidonate required calcium and was enhanced by diolein but did not require exogenous phosphatidylserine. Subma ... Full text Link to item Cite

The NADPH oxidase of human polymorphonuclear leukocytes. Evidence for regulation by multiple signals.

Journal Article J Biol Chem · May 10, 1984 Activation of the membrane-bound NADPH oxidase in human polymorphonuclear leukocytes can be triggered by chemoattractants, the tumor promoter phorbol myristate acetate or the calcium ionophore A23187. We have shown previously that these stimuli have marked ... Link to item Cite

Rapid changes in light scattering from human polymorphonuclear leukocytes exposed to chemoattractants. Discrete responses correlated with chemotactic and secretory functions.

Journal Article J Clin Invest · May 1984 A platelet aggregometer was adapted for the simultaneous measurement of perpendicular light scattering in addition to light transmission. The addition of chemoattractants to polymorphonuclear leukocyte suspensions evoked a single wave of increased light tr ... Full text Link to item Cite

Human malignant and mitogen-transformed cells contain retroviral P15E-related antigen.

Journal Article J Exp Med · March 1, 1984 Virus-related oncogenes have been demonstrated in human tumor cells and may play a role in neoplastic transformation. Cancerous effusions contain inhibitors of monocyte function and are absorbed by monoclonal antibodies to the immunosuppressive retroviral ... Full text Link to item Cite

Characterization of an oligopeptide chemoattractant receptor on human blood monocytes using a new radioligand.

Journal Article Blood · March 1984 The study of chemoattractant receptors on human monocytes had been limited by the lack of a radioligand suitable for use with the small numbers of cells routinely available from human donors. A new synthetic oligopeptide radioligand f[35S]met-leu-phe, with ... Link to item Cite

A chemoattractant receptor on macrophages exists in two affinity states regulated by guanine nucleotides.

Journal Article J Cell Biol · February 1984 The binding characteristics of the oligopeptide chemoattractant receptor on guinea pig macrophages and macrophage membrane preparations were characterized using detailed binding studies and computer analysis. Viable macrophages bound the radiolabeled chemo ... Full text Link to item Cite

A potential second messenger role for arachidonic acid: activation of Ca2+-dependent protein kinase.

Journal Article Trans Assoc Am Physicians · 1984 A widely distributed Ca2+- and phospholipid-dependent protein kinase, protein kinase C, may play a major role in cellular regulation. We now report that arachidonate can directly activate protein kinase C from human neutrophils. Activation was Ca2+-depende ... Link to item Cite

Chemoattractant receptors on phagocytic cells.

Journal Article Annu Rev Immunol · 1984 Chemoattractant receptors on leukocytes can trigger a number of cellular responses, including the cytoskeletal reorganization, changes in cell shape, directed motility, lysosomal enzyme secretion, and activation of the respiratory burst. The dose of chemoa ... Full text Link to item Cite

Transductional mechanisms of chemoattractant receptors on leukocytes.

Journal Article Contemp Top Immunobiol · 1984 Phagocytic leukocytes contain receptors for chemoattractants on their cell surface. Binding of chemotactic factors to these receptors initiates a number of coordinated cellular responses in a strict dose-dependent manner. Motility-related functions such as ... Full text Link to item Cite

CORRECTION

Journal Article IMMUNOLOGY TODAY · January 1, 1984 Link to item Cite

Pharmacologic manipulation of leukocyte chemotaxis. Present knowledge and future trends.

Journal Article Am J Med · October 31, 1983 The chemotactic responses of leukocytes are initiated by the binding of chemoattractants to specific cell-surface receptors. At larger doses, chemoattractants stimulate other biologic activities in leukocytes, including the production of superoxide anions ... Full text Link to item Cite

Murine malignant cells synthesize a 19,000-dalton protein that is physicochemically and antigenically related to the immunosuppressive retroviral protein, P15E.

Journal Article J Exp Med · September 1, 1983 Murine tumors contain low molecular weight factors that inhibit macrophage accumulation at inflammatory foci. Certain oncogenic murine leukemia viruses contain similar inhibitory activity and the active component of the retroviruses was shown to be the env ... Full text Link to item Cite

Guanine nucleotides modulate the binding affinity of the oligopeptide chemoattractant receptor on human polymorphonuclear leukocytes.

Journal Article J Clin Invest · September 1983 The oligopeptide chemoattractant receptor on human polymorphonuclear leukocyte (PMN) membranes exists in two affinity states. Since guanine nucleotides regulate the binding affinity and transductional activity of several other types of receptors, we examin ... Full text Link to item Cite

Induction of selective biological responses to chemoattractants in a human monocyte-like cell line.

Journal Article Infect Immun · September 1983 The availability of monocyte cell lines that can be induced to differentiate in a predictable fashion can provide important tools for the study of the biochemical mechanisms of specific cellular responses. The U937 human monocyte cell line was previously s ... Full text Link to item Cite

Polymorphonuclear leukocyte function in psoriasis: chemotaxis, chemokinesis, beta-adrenergic receptors, and proteolytic enzymes of polymorphonuclear leukocytes in the peripheral blood from psoriatic patients.

Journal Article J Invest Dermatol · September 1983 Psoriatic patients, particularly those with psoriatic arthritis, have neutrophilic and eosinophilic leukocytosis. Isolated polymorphonuclear leukocytes (PMNLs) from psoriatic patients have normal concentrations of proteolytic enzymes and they have beta-adr ... Full text Link to item Cite

Leukocyte chemotaxis.

Journal Article Fed Proc · August 1983 Link to item Cite

Activation of the respiratory burst enzyme in human polymorphonuclear leukocytes by chemoattractants and other soluble stimuli. Evidence that the same oxidase is activated by different transductional mechanisms.

Journal Article J Clin Invest · July 1983 Chemoattractant-receptor coupling triggers several biologic responses in phagocytic cells including activation of the respiratory burst. Prior evidence in intact cells implied that stimulation of the respiratory burst by chemoattractants was by a mechanism ... Full text Link to item Cite

Hormonal activation of adenylate cyclase in macrophage membranes is regulated by guanine nucleotides.

Journal Article J Immunol · February 1983 Many macrophage functions such as chemotaxis, phagocytosis, enzyme secretion, and cytotoxicity are influenced by intracellular cyclic nucleotide levels, but the regulatory mechanisms involved are poorly defined. We have developed methods that allowed us to ... Link to item Cite

Effect of membrane fluidizers on the number and affinity of chemotactic factor receptors on human polymorphonuclear leukocytes.

Journal Article Microbiol Immunol · 1983 Chemotaxis by leukocytes appears to be initiated by the binding of chemo-attractants to specific cell surface receptors. In other biological systems, the affinity and functional activity of membrane receptors are regulated by the local microviscosity. The ... Full text Link to item Cite

Chemoattractant receptor affinity reflects its ability to transduce different biological responses.

Journal Article Agents Actions Suppl · 1983 The oligopeptide chemotactic factor receptor in human PMN membranes exists in two affinity states which are in part interconvertible and regulated by guanine nucleotides. In whole cells, only one affinity of the receptor can be seen, presumably due to the ... Full text Link to item Cite

HUMAN-TUMOR CELLS AND MITOGEN-STIMULATED MONONUCLEAR-CELLS CONTAIN AN ANTIGEN RELATED TO RETROVIRAL P15E

Journal Article PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH · January 1, 1983 Link to item Cite

Amphotericin B alters the affinity and functional activity of the oligopeptide chemotactic factor receptor on human polymorphonuclear leukocytes.

Journal Article J Immunol · October 1982 Leukocyte chemotaxis is initiated by the binding of chemotactic factors to specific, high-affinity receptors. Amphotericin B, a polyene antibiotic that binds to membrane cholesterol, inhibits human neutrophil (PMN) chemotaxis. We examined the effects of th ... Link to item Cite

Chemoattractant receptor functions in human polymorphonuclear leukocytes are divergently altered by membrane fluidizers.

Journal Article Proc Natl Acad Sci U S A · October 1982 The chemotactic factor receptor on leukocytes initiates several cellular responses including chemotaxis, lysosomal enzyme secretion, and O2- production. The latter two responses require approximately 10-100 times more chemoattractant than is required for c ... Full text Link to item Cite

Cyclic nucleotides regulate the morphologic alterations required for chemotaxis in monocytes.

Journal Article J Immunol · March 1982 The initial morphologic response of human monocytes to chemoattractants is a change in shape from round to a triangular "motile" configuration (polarization). At doses chemotactic in vitro, chemoattractants induced rapid (t 1/2 = 45 sec), sustained (greate ... Link to item Cite

Induction of cellular immunity to Coccidioides immitis after sensitization with dinitrochlorobenzene.

Journal Article Ann Intern Med · March 1982 A patient had progressive disseminated coccidioidomycosis and depressed cellular immunity to Coccidioides immitis. He developed a large nasal coccidioidal lesion that was unresponsive to conventional therapy. Application of dinitrochlorobenzene (DNCB) to t ... Full text Link to item Cite

Transmethylation reactions regulate affinity and functional activity of chemotactic factor receptors on macrophages.

Journal Article Cell · January 1982 Methylation mediated by S-adenosyl-L-methionine is required for the chemotaxis of mononuclear leukocytes. We investigated whether transmethylation reactions are required for normal functioning of chemotactic factor receptors. Three chemoatractant-mediated ... Full text Link to item Cite

Effects of interferon therapy on monocyte functions of cancer patients

Journal Article Proceedings of the American Association for Cancer Research · January 1, 1982 Cite

EFFECTS OF INTERFERON THERAPY ON MONOCYTE FUNCTIONS OF CANCER-PATIENTS

Journal Article PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH · January 1, 1982 Link to item Cite

Alterations of new methylated phospholipid synthesis in the plasma membranes of macrophages exposed to chemoattractants.

Journal Article J Cell Biol · October 1981 Chemotactic factors have been shown to inhibit the methylation of phosphatidylethanolamine in macrophages without affecting total phospholipid synthesis. It would thus be anticipated that newly synthesized membranes of macrophages exposed to chemoattractan ... Full text Link to item Cite

Transmethylation reactions are required for initial morphologic and biochemical responses of human monocytes to chemoattractants.

Journal Article J Immunol · October 1981 Transmethylation reactions mediated by S-adenosyl-L-methionine are required for the chemotaxis of mononuclear leukocytes. It is not yet known, however, whether methylation reactions participate in the initial transduction of the chemotactic signals that le ... Link to item Cite

Inhibitors of monocyte responses to chemotaxins are present in human cancerous effusions and react with monoclonal antibodies to the P15(E) structural protein of retroviruses.

Journal Article J Clin Invest · October 1981 Individuals with cancer have previously been shown to have abnormal chemotactic responsiveness. Surgical removal of the tumor often resulted in normalization of monocyte function, which suggests that human neoplasms might inhibit monocyte chemotaxis by rel ... Full text Link to item Cite

Molecular and cellular mechanisms of leukocyte chemotaxis.

Journal Article Science · August 21, 1981 The application of modern scientific methods to the study of leukocyte function has begun to reveal the molecular and cytostructural bases of the chemotactic responses of these cells. Leukocyte chemotaxis is initiated by the binding of chemoattractants to ... Full text Link to item Cite

The role of transmethylation reactions in regulating the binding of BCG-activated murine macrophages to neoplastic target cells.

Journal Article J Immunol · July 1981 The ability of BCG-activated macrophages from C57BL/6J mice to lyse neoplastic targets was depressed by inhibitors of methyltransferase reactions (10(-4) M adenosine, 10(-5) M EHNA, and 10(-4) M L-homocysteine or 10(-5) M DZA). Binding of P815 mastocytoma ... Link to item Cite

Differential anti-inflammatory effects of LPS in susceptible and resistant mouse strains.

Journal Article J Immunol · July 1981 Most mouse strains are highly susceptible to endotoxin (LPS) lethality and are responsive to LPS stimulation in vitro (e.g., B cell mitogenesis, macrophage activation). They are, however, capable of mounting only a small inflammatory response to LPS when i ... Link to item Cite

Monocyte responsiveness to chemotactic stimuli is a property of a subpopulation of cells that can respond to multiple chemoattractants.

Journal Article J Clin Invest · January 1981 The chemotactic migration of leukocytes is preceded by an alteration in the cells' shape from round to a characteristic polar configuration. We have developed an assay that shows that human monocytes, when exposed to chemoattractant in suspension, assume t ... Full text Link to item Cite

Disorders of human leukocyte chemotaxis.

Journal Article Clin Immunol Rev · 1981 Link to item Cite

Effects of amphotericin B on a human neutrophil chemotactic factor receptor

Journal Article Federation Proceedings · January 1, 1981 Cite

POLYMORPHONUCLEAR LEUKOCYTE FUNCTIONS IN PSORIASIS

Journal Article CLINICAL RESEARCH · January 1, 1981 Link to item Cite

Depression of murine macrophage accumulation by low-molecular-weight derived from spontaneous mammary carcinomas.

Journal Article J Natl Cancer Inst · October 1980 Extracts prepared from spontaneous mouse mammary adenocarcinomas, as well as plasma and urine from inbred C3H/HeN mice carrying murine mammary tumor virus and bearing such tumors, significantly inhibited the accumulation of macrophages at inflammatory site ... Full text Link to item Cite

N-Formylmethionyl peptide receptors on equine leukocytes initiate secretion but not chemotaxis.

Journal Article Science · July 25, 1980 The chemotaxis of leukocytes appears to be initiated by the binding of chemotactic factors to the surface of these cells. N-Formylated peptides induce chemotaxis and lysosomal enzyme secretion of leukocytes; because these peptides are available in a purifi ... Full text Link to item Cite

Development of specific receptors for N-formylated chemotactic peptides in a human monocyte cell line stimulated with lymphokines.

Journal Article J Exp Med · July 1, 1980 A human monocyte-like cell line, U937, when grown in continuous culture, does not secrete lysosomal enzymes or migrate towards chemotactic factors. When the cells are stimulated by lymphokines, however, they develop the ability both to migrate directionall ... Full text Link to item Cite

Macrophage accumulation in mice is inhibited by low molecular weight products from murine leukemia viruses.

Journal Article J Immunol · June 1980 Low m.w. extracts from three known oncogenic viruses, Friend, Moloney, and Rauscher, inhibited the accumulation of macrophages at sites of delayed inflammatory reactions in mice. The potential biologic significance of these proteins is suggested by their p ... Link to item Cite

Demonstration of a chemotactic factor receptor on macrophages.

Journal Article J Immunol · June 1980 Certain synthetic N-formylated peptides are potent chemotactic agents for phagocytic cells. We have identified a specific, high affinity receptor for the chemotactic peptide fMet-Leu-[3H]Phe on inflammatory as well as on resident guinea pig peritoneal macr ... Link to item Cite

Influence of cytoskeletal assembly on phosphatidylcholine synthesis in intact phagocytic cells.

Journal Article Cell · June 1980 Interactions of the plasma membrane with the cytoskeleton are required for diverse cellular functions such as adhesion, division, secretion, endocytosis and chemotaxis. We therefore investigated whether the reversible assembly of microtubules and/or microf ... Full text Link to item Cite

An inherited abnormality of neutrophil adhesion. Its genetic transmission and its association with a missing protein.

Journal Article N Engl J Med · May 22, 1980 Neutrophils from a five-year-old boy with recurrent bacterial infections failed to spread on surfaces, leading to a severe defect in chemotaxis and a mild impairment in phagocytosis. Failure to spread was also seen in a fraction of the neutrophils from the ... Full text Link to item Cite

Absence of macrophage involvement in the passive serum therapy of Friend leukemia virus-induced disease.

Journal Article Cancer Res · March 1980 The possible involvement of host macrophages in the passive serum therapy of Friend leukemia virus (FLV)-induced disease has been examined with the use of agents inhibiting normal macrophage functions, including silica and a tumor-produced macrophage chemo ... Link to item Cite

DEPRESSION OF MURINE MACROPHAGE ACCUMULATION BY FACTORS DERIVED FROM SPONTANEOUS MAMMARY CARCINOMAS

Journal Article PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH · January 1, 1980 Link to item Cite

MACROPHAGE CHEMOTAXIS INHIBITOR DERIVED FROM GUINEA-PIG HEPATO-CARCINOMA

Journal Article FEDERATION PROCEEDINGS · January 1, 1980 Link to item Cite

FIBROBLAST CHEMOATTRACTANTS

Journal Article FEDERATION PROCEEDINGS · January 1, 1980 Link to item Cite

Chronic mucocutaneous candidiasis. Immunologic studies of three generations of a single family

Journal Article American Journal of Medicine · December 1, 1979 A family consisting of eight members in three generations (age 10 months to 53 years) affected with chronic mucocutaneous candidiasis was studied along with three unaffected relatives. Dermatophytosis, loss of teeth and recurrent viral infections were pres ... Full text Cite

Chronic mucocutaneous candidiasis. Immunologic studies of three generations of a single family.

Journal Article Am J Med · December 1979 A family consisting of eight members in three generations (age 10 months to 53 years) affected with chronic mucocutaneous candidiasis was studied along with three unaffected relatives. Dermatophytosis, loss of teeth and recurrent viral infections were pres ... Full text Link to item Cite

Generation of a fibroblast chemotactic factor in serum by activation of complement.

Journal Article J Clin Invest · November 1979 When serum complement is activated by either the classical or alternative pathways, a factor with an apparent 80,000 mol wt is generated that is chemotactic for human dermal fibroblasts. The origin of this serum-derived chemotactic factor (SDCF) is not kno ... Full text Link to item Cite

Deficiency of the fifth component of complement in human subjects. Clinical, genetic and immunologic studies in a large kindred.

Journal Article Am J Med · October 1979 The discovery of a large kindred with a heritable deficiency of the fifth component of complement (C5) has permitted the accumulation of new clinical, genetic and immunologic data concerning the role of C5 in human subjects. The proband, who has had nine e ... Full text Link to item Cite

Phospholipid methylation in macrophages is inhibited by chemotactic factors.

Journal Article Proc Natl Acad Sci U S A · June 1979 Chemotaxis by human monocytes has been shown to require methylation mediated by S-adenosyl-L-methionine(AdoMet), but the specific transmethylation reaction necessary for this function was not elucidated. In an attempt to define the methylation requirement ... Full text Link to item Cite

Do macrophages destroy nascent tumors?

Journal Article J Natl Cancer Inst · June 1979 Link to item Cite

Neisseria meningitidis bacteremia in association with deficiency of the sixth component of complement.

Journal Article Infect Immun · June 1979 The serum of a 26-year-old black man with a recent episode of meningococcemia complicated by meningitis and arthritis was found to lack hemolytic complement activity. The sixth component of complement was not detected by functional or immunochemical assays ... Full text Link to item Cite

Neisseria meningitidis and Neisseria gonorrhoeae bacteremia associated with C6, C7, or C8 deficiency.

Journal Article Ann Intern Med · June 1979 We summarize data from 24 previously described or newly diagnosed cases of homozygous deficiency of the sixth, seventh, or eighth components of complement. Thirteen of 24 patients had at least one episode, and usually two or more episodes of Neisseria meni ... Full text Link to item Cite

Antibodies raised against purified beta-adrenergic receptors specifically bind beta-adrenergic ligands.

Journal Article Proc Natl Acad Sci U S A · May 1979 Antibodies raised against purified beta-adrenergic receptors themselves specifically bind beta-adrenergic ligands. Digitonin-solubilized frog (Rana pipiens) erythrocyte beta-adrenergic receptors, purified 100- to 200-fold by adsorption to an alprenolol-aga ... Full text Link to item Cite

Mechanism of chemoattraction by mouse skin neutral proteinase

Journal Article Journal of Investigative Dermatology · January 1, 1979 Cite

Transmethylation reactions and the chemotaxis of eukaryotic cells

Journal Article Journal of Supramolecular and Cellular Biochemistry · January 1, 1979 Cite

Hereditary C2 deficiency associated with cutaneous lupus erythematosus: clinical, laboratory, and genetic studies.

Journal Article Arch Dermatol · January 1979 Selective congenital deficiency in the second component of complement has been described in association with lupus erythematosus (LE) and other connective tissue disorders. We identified a 59-year-old woman with a 13-year history of cutaneous LE and no det ... Link to item Cite

Rheumatoid nodulosis. Sporadic and familial diseases.

Journal Article J Rheumatol · 1979 Two patients are described with longstanding, multiple, subcutaneous nodules with the histopathological features of rheumatoid nodules. Neither patient had any clinical evidence of rheumatic disease. One patient had a family history of smiliar nodules tran ... Link to item Cite

TRANSMETHYLATION REACTIONS AND THE CHEMOTAXIS OF EUKARYOTIC CELLS

Conference JOURNAL OF SUPRAMOLECULAR STRUCTURE · January 1, 1979 Link to item Cite

MECHANISM OF CHEMO-ATTRACTION BY MOUSE SKIN NEUTRAL PROTEINASE

Journal Article FEDERATION PROCEEDINGS · January 1, 1979 Link to item Cite

Monocyte chemotactic mechanisms in patients with hypernephroma.

Journal Article Natl Cancer Inst Monogr · December 1978 Peripheral blood monocyte chemotaxis was evaluated in 15 patients with hypernephroma, 10 of whom were evaluated preoperatively and postoperatively. Five patients had a significantly reduced response preoperatively that returned to normal levels after radic ... Link to item Cite

Familial deficiency of the seventh component of complement associated with recurrent bacteremic infections due to Neisseria.

Journal Article J Infect Dis · September 1978 The serum of a 29-year old woman with a recent episode of disseminated gonococcal infection and a history of meningococcal meningitis and arthritis as a child was found to lack serum hemolytic complement activity. The seventh component of complement (C7) w ... Full text Link to item Cite

Tubular arrays on electron microscopy not specific.

Journal Article N Engl J Med · August 10, 1978 Full text Link to item Cite

Requirement of S-adenosyl-L-methionine-mediated methylation for human monocyte chemotaxis.

Journal Article Proc Natl Acad Sci U S A · August 1978 The chemotactic response of motile bacteria requires the methylation of specific proteins by S-adenosyl-L-methionine. To determine whether methylation is required for the chemotaxis of human leukocytes, we studied the effects of inhibition of S-adenosyl-L- ... Full text Link to item Cite

Resistance of neoplasms to immunological destruction: role of a macrophage chemotaxis inhibitor.

Journal Article J Exp Med · July 1, 1978 Several tissue culture lines of 6C3HED, a murine lymphoma, were more susceptible to immunologic destruction in vivo than the highly virulent 6C3HED line maintained by serial intramuscular transplantation. The attenuated tissue culture cells were rejected b ... Full text Link to item Cite

Abortive and productive infections of human mononuclear phagocytes by type I herpes simplex virus.

Journal Article Am J Pathol · April 1978 The ability of Type I herpes simplex (HSV) to replicate in normal human monouclear phagocytes was investigated. Mononuclear leukocytes were obtained from the peripheral blood of patients by Ficoll-Hypaque gradient centrifugation, and the monocytes were iso ... Link to item Cite

Abnormal monocyte chemotaxis in patients with breast cancer: evidence for a tumor-mediated effect.

Journal Article J Natl Cancer Inst · April 1978 The chemotactic responsiveness of peripheral blood monocytes was measured in 194 individuals: 37 patients with breast cancer, 17 patients with a history of breast cancer but clinically free of disease after surgery, 42 patients with benign breast masses, a ... Full text Link to item Cite

The role of macrophages in the rheumatic diseases

Journal Article Clinics in Rheumatic Diseases · January 1, 1978 In this article the functions of the macrophage are reviewed and a role for the macrophage in rheumatic disease is proposed. The known mechanisms by which mononuclear phagocytes are mobilised from the bone marrow as a result of peripheral macrophages makin ... Cite

REQUIREMENT OF METHYLATION FOR HUMAN MONOCYTE CHEMOTAXIS

Journal Article FEDERATION PROCEEDINGS · January 1, 1978 Link to item Cite

CHARACTERIZATION OF A TUMOR DERIVED MACROPHAGE CHEMOTAXIS INHIBITOR (MCI)

Journal Article FEDERATION PROCEEDINGS · January 1, 1978 Link to item Cite

MUCO-CUTANEOUS CANDIDIASIS - IMMUNOLOGICAL STUDIES IN 3 GENERATIONS

Journal Article CLINICAL RESEARCH · January 1, 1978 Link to item Cite

MUCO-CUTANEOUS CANDIDIASIS - IMMUNOLOGICAL STUDIES IN 3 GENERATIONS

Conference JOURNAL OF INVESTIGATIVE DERMATOLOGY · January 1, 1978 Link to item Cite

Generalized microsporum audoninii infection and depressed cellular immunity associated with a missing plasma factor required for lymphocyte blastogenesis.

Journal Article Am J Med · December 1977 Described herein is a 15 year old girl with a generalized, possibly systemic Microsporum audouinni infectin associated with anergy and defective lymphocyte transformation as a consequence of a deficiency of an uncharacterized plasma factor. Intravenous adm ... Full text Link to item Cite

Macrophage migratory dysfunction in cancer. A mechanism for subversion of surveillance.

Journal Article Am J Pathol · September 1977 There is considerable evidence to suggest that macrophages participate in host resistance to the development and spread of cancer. We have, therefore, studied monocytemacrophage function in humans and animals with neoplasms. Approximately 60% of patients w ... Link to item Cite

Abnormalities of leukotaxis in atopic dermatitis.

Journal Article J Allergy Clin Immunol · August 1977 Leukocyte chemotaxis studies were performed in 14 patients with atopic dermatitis. Monocyte chemotactic responsiveness (MCR), polymorphonuclear leukocyte (PMN) chemotactic responsiveness (PCR), and patient serum inhibition of normal monocyte chemotaxis wer ... Full text Link to item Cite

Disorders of leukocyte chemotaxis.

Journal Article Pediatr Clin North Am · May 1977 The rapid accumulation of inflammatory cells at sites of microbial invasion or neoplastic transformation is a central event in immunologically-mediated host defense. The availability of methodology to accurately quantify leukocyte migration in vitro has al ... Full text Link to item Cite

Specific receptor sites for chemotactic peptides on human polymorphonuclear leukocytes.

Journal Article Proc Natl Acad Sci U S A · March 1977 Synthetic N-formylmethionyl peptides are chemotactic attractants for human polymorphonuclear leukocytes. The well-defined structure-activity relationship of these peptides in eliciting a chemotactic response suggests that the interaction of the peptides wi ... Full text Link to item Cite

Abnormalitieis of monocyte chemotaxis in patients with melanoma: effects of immunotherapy and tumor removal.

Journal Article J Natl Cancer Inst · January 1977 The chemotactic responsiveness of peripheral blood monocytes was studied before and after immunotherapy was administered to 56 patients with melanoma. Abnormal chemotaxis was found in 36 patients (64%) prior to treatment; this abnormality correlated with s ... Full text Link to item Cite

Influenza induced depression of human monocyte chemotaxis reversed by levamisole

Journal Article Federation Proceedings · January 1, 1977 Cite

Influenza-induced depression of monocyte chemotaxis: Reversal by levamisole

Journal Article Cellular Immunology · January 1, 1977 Depression of monocyte chemotactic responsiveness that occurs in patients with acute influenza may be a factor in causing the high incidence of superinfection seen in this viral disease. Levamisole, a pharmacological agent capable of enhancing monocyte che ... Full text Cite

FAMILIAL DEFICIENCY OF 5TH COMPONENT OF COMPLEMENT

Journal Article CLINICAL RESEARCH · January 1, 1977 Link to item Cite

DEPRESSION OF HUMAN MONOCYTE CHEMOTAXIS BY AMNIOTIC-FLUID

Journal Article CLINICAL RESEARCH · January 1, 1977 Link to item Cite

An inhibitor of macrophage function produced by neoplasms

Journal Article Cellular Immunology · December 1976 Full text Cite

The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor.

Journal Article J Exp Med · November 2, 1976 A quantitative assay that measures fibroblast chemotaxis in vitro is described. Application of this technique has revealed that peripheral blood lymphocytes stimulated by antigen or mitogen in vitro produce a factor that is chemotactic for human dermal fib ... Full text Link to item Cite

Human lymphocyte motility: normal characteristics and anomalous behavior of chronic lymphocytic leukemia cells.

Journal Article Blood · November 1976 The characteristics of human lymphocyte motility and its relationship to the redistribution of surface membrane antigens (capping) are poorly defined. Since chronic lymphocytic leukemia (CLL) cells cap poorly when compared with normal human lymphocytes, th ... Link to item Cite

Effect of virus infection on the inflammatory response. Depression of macrophage accumulation in influenza-infected mice.

Journal Article Am J Pathol · November 1976 To better define the mechanisms by which viruses depress immune function, the effect of influenza infection on the ability of macrophages to accumulate at sites of inflammation was determined. Mice were inoculated with virus, and their inflammatory respons ... Link to item Cite

Depression of macrophage function by a factor produced by neoplasms: a merchanism for abrogation of immune surveillance.

Journal Article J Immunol · October 1976 The possibility that macrophages mediate surveillance against the development of neoplasms has been reciving increasing support. The acquisition, by neoplastic cells, of the capacity to subvert macrophage function may be an important mechanism by which the ... Link to item Cite

Calcium influx requirement for human neutrophil chemotaxis: inhibition by lanthanum chloride.

Journal Article Science · September 3, 1976 Calcium fluxes of human neutrophils measured in the presence of chemotactically active serum showed a marked stimulation of calcium-45 uptake from the media. Chemotactically inactive serum did not cause an influx of calcium. The magnitude of the calcium in ... Full text Link to item Cite

Prostaglandins and inflammation: enhancement of monocyte chemotactic responsiveness by prostaglandin E2.

Journal Article Prostaglandins · September 1976 The effects of prostaglandins on human monocyte chemotaxis were studied in vitro. None of the prostaglandins tested, including members of the A, B, E or F series, were chemotactic for monocytes. Prostaglandin E2 however, enhanced the chemotactic responsive ... Full text Link to item Cite

A role for adenosine deaminase in human monocyte maturation.

Journal Article J Clin Invest · August 1976 The occurrence of a deficiency of adenosine deaminase (ADA) activity in some patients with severe combined immunodeficiency suggests a possible relationship between the activity of ADA and the aberration of the immune system. To help delineate the function ... Full text Link to item Cite

An inhibitor of macrophage chemotaxis produced by neoplasms.

Journal Article Science · April 23, 1976 The accumulation of macrophages at neoplastic sites may be an important event in immunologically mediated tumor killing. The implantation of syngeneic neoplasms in mice, however, was found to depress the animal's ability to localize macrophages at inflamma ... Full text Link to item Cite

Effects of neoplasms on inflammation: depression of macrophage accumulation after tumor implantation.

Journal Article J Immunol · March 1976 The local accumulation of macrophages at sites of neoplasms may be a critical event in immunologically mediated tumor killing. Individuals with neoplasms, however, have been noted to have depressed monocyte chemotactic responsiveness in vitro. To determine ... Link to item Cite

Identification of beta-adrenergic receptors in human lymphocytes by (-) (3H) alprenolol binding.

Journal Article J Clin Invest · January 1976 Human lymphocytes are known to posessess a catecholamine-responsive adenylate cyclase which has typical beta-adrenergic specificity. To identify directly and to quantitate these beta-adenergic receptors in human lymphocytes, (-) [3H] alprenolol, a potent b ... Full text Open Access Link to item Cite

INFLAMMATORY RESPONSE AND RESISTANCE TO ENDOTOXIN (LPS) IN MICE

Journal Article CLINICAL RESEARCH · January 1, 1976 Link to item Cite

ADENOSINE-DEAMINASE AND HUMAN MONOCYTE-MACROPHAGE MATURATION

Journal Article JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY · January 1, 1976 Link to item Cite

DEPRESSED MACROPHAGE ACCUMULATION FOLLOWING INFLUENZA INFECTION IN MICE

Journal Article AMERICAN JOURNAL OF PATHOLOGY · January 1, 1976 Link to item Cite

INHIBITOR OF MACROPHAGE CHEMOTAXIS PRODUCED BY NEOPLASMS

Conference CLINICAL RESEARCH · January 1, 1976 Link to item Cite

IDENTIFICATION OF A MACROPHAGE CHEMOTAXIS INHIBITOR PRODUCED BY NEOPLASMS

Journal Article FEDERATION PROCEEDINGS · January 1, 1976 Link to item Cite

Depressed monocyte chemotaxis during acute influenza infection.

Journal Article Lancet · November 29, 1975 The chemotactic responsiveness of monocytes from patients with serologically proven influenza infection has been quantified in vitro. Individuals with acute influenza had a significant (P less than 0-001) depression of monocyte chemotaxis. The depression r ... Full text Link to item Cite

Defective monocyte function in patients with genitourinary carcinoma.

Journal Article J Natl Cancer Inst · November 1975 An assay allowing quantitation of monocyte function, i.e., chemotaxis, was used to study the activity of monocytes in patients with neoplasms of the urinary tract. Twenty-four subjects with various stages of renal carcinoma exhibited a mean chemotactic def ... Full text Link to item Cite

Abnormalities of leukocyte chemotaxis in human disease.

Journal Article Ann N Y Acad Sci · June 13, 1975 Full text Link to item Cite

Quantification of mouse macrophage chemotaxis in vitro: role of C5 for the production of chemotactic activity.

Journal Article Infect Immun · March 1975 Delineation of the mechanisms of macrophage accumulation at local tissue sites will further our understanding of immunologically mediated host resistance to infectious and neoplastic diseases. Since mice are frequently used for the study of immune function ... Full text Link to item Cite

Interaction of complex polysaccharides with the complement system: effect of calcium depletion on terminal component consumption.

Journal Article Infect Immun · February 1975 Complex polysaccharides and lipopolysaccharides can activate the terminal components of complement by either the classical (antibody, C1, C4, and C2) or alternative complement pathways, but the relative importance of either pathway for terminal component c ... Full text Link to item Cite

Depression of macrophage chemotaxis in vivo in tumor bearing mice

Journal Article Federation Proceedings · January 1, 1975 Cite

Characterization of chemotactic activity produced in vivo by a cell mediated immune reaction in the guinea pig

Journal Article Journal of Immunology · January 1, 1975 A system that allows repeated sampling of peritoneal fluid at various time intervals has been adapted to study mechanisms of leukocyte accumulation in vivo. Application of this technique in guinea pigs exhibiting delayed hypersensitivity (DH) to horseradis ... Cite

Characterization of chemotactic activity produced in vivo by a cell-mediated immune reaction in the guinea pig.

Journal Article J Immunol · January 1975 A system that allows repeated sampling of peritoneal fluid at various time intervals has been adapted to study mechanisms of leukocyte accumulation in vivo. Application of this technique in guinea pigs exhibiting delayed hypersensitivity (DH) to horse radi ... Link to item Cite

DEPRESSION OF MACROPHAGE CHEMOTAXIS INVIVO IN TUMOR BEARING MICE

Journal Article FEDERATION PROCEEDINGS · January 1, 1975 Link to item Cite

DEPRESSION OF MONOCYTE CHEMOTAXIS BY NEOPLASMS

Journal Article CLINICAL RESEARCH · January 1, 1975 Link to item Cite

Abnormalities of chemotactic lymphokine synthesis and mononuclear leukocyte chemotaxis in Wiskott-Aldrich syndrome.

Journal Article J Clin Invest · August 1974 Wiskott-Aldrich syndrome is characterized by numerous humoral and cellular immune abnormalities including anergy, defective antibody production, and increased immunoglobulin synthesis. To define better the mechanisms of defective cellular immunity in this ... Full text Link to item Cite

Production of chemotactic factor and lymphotoxin by human leukocytes stimulated with Herpes simplex virus.

Journal Article Infect Immun · July 1974 Peripheral blood leukocytes (PBL) of 41 subjects were tested for their ability to be stimulated by herpes simplex virus antigens as measured by [(3)H]thymidine incorporation and lymphokine production (i.e., lymphocyte-derived chemotactic factor and lymphot ... Full text Link to item Cite

Role for C4 in the activation of complement (C) by endotoxin

Journal Article Federation Proceedings · January 1, 1974 Cite

Chemotaxis of mononuclear leukocytes.

Journal Article Antibiot Chemother (1971) · 1974 Full text Link to item Cite

Enhancement of human mononuclear leukocyte chemotaxis by prostaglandin E2

Journal Article Clinical Research · January 1, 1974 Cite

Cytogenetic engineering in vivo: restoration of biologic complement activity to C5-deficient mice by intravenous inoculation of hybrid cells.

Journal Article Proc Natl Acad Sci U S A · November 1973 Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5-deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These ... Full text Link to item Cite

Activation of complement by endotoxin.

Journal Article J Infect Dis · July 1973 Full text Link to item Cite

Bone marrow-derived lymphoid cells (B cells): functional depletion with cobra factor and fresh serum.

Journal Article Science · November 24, 1972 Treatment of rat spleen cells with cobra factor and fresh rat serum provided a simple, rapid means of functionally eliminating complement receptor lymphocytes. Cells able to differentiate into plaque-forming cells in a syngeneic, irradiated host were dimin ... Full text Link to item Cite

Further characterization of a factor from endotoxin-treated serum which releases histamine and heparin from mast cells.

Journal Article Infect Immun · June 1972 Upon incubation of hamster serum with bacterial endotoxin, a factor is produced which releases histamine and heparin from hamster mast cells and increases capillary permeability in guinea pig skin. The major histamine-releasing activity derived from hamste ... Full text Link to item Cite

Instrinsic defect of the polymorphonuclear leucocyte resulting in impaired chemotaxis and phagocytosis.

Journal Article Clin Exp Immunol · December 1971 A patient is presented who has recurrent infections associated with a cellular defect of PMN chemotaxis and phagocytosis, as well as a sex-linked form of congenital agammaglobulinaemia. The impairments of PMN function were demonstrated in vitro by an inabi ... Link to item Cite

Biological activity of complement in vivo. Role of C5 in the accumulation of polymorphonuclear leukocytes in inflammatory exudates.

Journal Article J Exp Med · November 1, 1971 The importance of C5 in the generation of complement (C)-dependent chemotactic activity in vitro is well recognized. However, the actual role C5 may play in the accumulation of polymorphonuclear leukocytes (PMN) at inflammatory sites in vivo has not been e ... Full text Link to item Cite

A chemotactic factor for mononuclear leukocytes.

Journal Article Proc Soc Exp Biol Med · November 1971 Full text Link to item Cite

Inflammation and herpes simplex virus: release of a chemotaxis-generating factor from infected cells.

Journal Article Science · December 4, 1970 Infection of primary rabbit kidney cells with herpes simplex virus leads to the release of a cell factor or factors that upon incubation with serum results in the cleavage of the fifth component, C5, of complement. The product of this cleavage, C5a, is che ... Full text Link to item Cite

Histamine-releasing factor generated by the interaction of endotoxin with hamster serum.

Journal Article Infect Immun · October 1970 Injection of hamsters with endotoxin (LPS) resulted, 4 to 14 days later, in a marked decline in the mast cell count and in a reduction of the available histamine content of cells obtained by peritoneal lavage. The release of histamine from isolated periton ... Full text Link to item Cite

Polymorphonuclear leukocyte chemotactic activity in rabbit serum and Guinea pig serum treated with immune complexes: evidence for c5a as the major chemotactic factor.

Journal Article Infect Immun · June 1970 A number of polymorphonuclear leukocyte (PMN) chemotactic factors derived from complement components have been described. We sought to determine which of these factors accounted for the majority of PMN chemotactic activity in rabbit serum and guinea pig se ... Full text Link to item Cite

Interactions of the complement system with endotoxic lipopolysaccharides in immunoglobulin-deficient sera.

Journal Article J Exp Med · April 1, 1970 Bacterial lipopolysaccharides (LPS) derived from a variety of organisms effectively induced C consumption in humans, bovines, and porcines with developmental agammaglobulinemia; birds with experimental agammaglobulinemia; and humans with agammaglobulinemia ... Full text Link to item Cite

Neutralization of sensitized virus by purified components of complement.

Journal Article Proc Natl Acad Sci U S A · March 1970 Herpes simplex virus which had been sensitized with immunoglobulin M antibody was neutralized by serum deficient in the fifth and sixth components of complement (C) but not by serum deficient in the fourth component C (C4). The sequential addition of the f ... Full text Link to item Cite

Neutralization of sensitized virus by the fourth component of complement.

Journal Article Science · August 1, 1969 Herpes simplex virus which had been sensitized with IgM antibody was not neutralized by the addition of the purified activated first component of complement. In the presence of an optimum concentration of the first component of complement, however, the sen ... Full text Link to item Cite

Leukocyte chemotactic activity from oral bacteria.

Journal Article J Periodontal Res Suppl · 1969 Link to item Cite

Decreased properdin activity in acute glomerulonephritis.

Journal Article Int Arch Allergy Appl Immunol · 1969 Full text Link to item Cite

Chemotactic and anaphylatoxic fragment cleaved from the fifth component of guinea pig complement.

Journal Article Science · October 18, 1968 The fifth component of guinea pig complement, with a sedimentation coefficient 7.8S, is cleaved by sensitized sheep erythrocytes treated with the first four components of complement into two fragments with sedimentation coefficients of 7.4S and 1.5S. The s ... Full text Link to item Cite

Interactions of the complement system with endotoxic lipopolysaccharide. Generation of a factor chemotactic for polymorphonuclear leukocytes.

Journal Article J Exp Med · August 1, 1968 Endotoxic lipopolysaccharide has recently been shown to fix large amounts of the complement components related to the biologic activities mediated by that system. The present study sought to determine whether the generation of chemotactic factor by endotox ... Full text Link to item Cite

Decreased C'-1q protein concentration and agglutinating activity in agammaglobulinaemia syndromes: an inborn error reflected in the complement system.

Journal Article Clin Exp Immunol · June 1968 In both C'1q protein and agglutination assays, markedly reduced mean titres (27 and <10% normal, respectively) were observed in Swiss type lymphopenic agammaglobulinaemia. Mean C'1q titres were somewhat decreased in other forms of agammaglobulinaemia, but ... Link to item Cite

Personalized Health Planning in Veterans Health Administration Primary Care Settings: A Model for Patient Centered Care.

Journal Article Federal practitioner : for the health care professionals of the VA, DoD, and PHS Cite