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Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study.

Publication ,  Journal Article
Berger, M; Murdoch, DM; Staats, JS; Chan, C; Thomas, JP; Garrigues, GE; Browndyke, JN; Cooter, M; Quinones, QJ; Mathew, JP; Weinhold, KJ ...
Published in: Anesth Analg
November 2019

Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.

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Published In

Anesth Analg

DOI

EISSN

1526-7598

Publication Date

November 2019

Volume

129

Issue

5

Start / End Page

e150 / e154

Location

United States

Related Subject Headings

  • Receptors, IgG
  • Postoperative Cognitive Complications
  • Pilot Projects
  • Monocytes
  • Lipopolysaccharide Receptors
  • Humans
  • GPI-Linked Proteins
  • Flow Cytometry
  • Cerebrospinal Fluid
  • Anesthesiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Berger, M., Murdoch, D. M., Staats, J. S., Chan, C., Thomas, J. P., Garrigues, G. E., … MADCO-PC Study Team, . (2019). Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study. Anesth Analg, 129(5), e150–e154. https://doi.org/10.1213/ANE.0000000000004179
Berger, Miles, David M. Murdoch, Janet S. Staats, Cliburn Chan, Jake P. Thomas, Grant E. Garrigues, Jeffrey N. Browndyke, et al. “Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study.Anesth Analg 129, no. 5 (November 2019): e150–54. https://doi.org/10.1213/ANE.0000000000004179.
Berger M, Murdoch DM, Staats JS, Chan C, Thomas JP, Garrigues GE, et al. Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study. Anesth Analg. 2019 Nov;129(5):e150–4.
Berger, Miles, et al. “Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study.Anesth Analg, vol. 129, no. 5, Nov. 2019, pp. e150–54. Pubmed, doi:10.1213/ANE.0000000000004179.
Berger M, Murdoch DM, Staats JS, Chan C, Thomas JP, Garrigues GE, Browndyke JN, Cooter M, Quinones QJ, Mathew JP, Weinhold KJ, MADCO-PC Study Team. Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study. Anesth Analg. 2019 Nov;129(5):e150–e154.

Published In

Anesth Analg

DOI

EISSN

1526-7598

Publication Date

November 2019

Volume

129

Issue

5

Start / End Page

e150 / e154

Location

United States

Related Subject Headings

  • Receptors, IgG
  • Postoperative Cognitive Complications
  • Pilot Projects
  • Monocytes
  • Lipopolysaccharide Receptors
  • Humans
  • GPI-Linked Proteins
  • Flow Cytometry
  • Cerebrospinal Fluid
  • Anesthesiology