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Brigid L. M. Hogan

Brigid L. M. Hogan

Research Professor of Cell Biology
Cell Biology
brigid.hogan@duke.edu
Duke Box 3709, Durham, NC 27710
424 Jones Bldg, 207 Research Drive, Durham, NC 27710
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Selected Publications

Dorso-ventral heterogeneity in tracheal basal stem cells.

Journal Article Biol Open · September 15, 2021 The tracheal basal cells (BCs) function as stem cells to maintain the epithelium in steady state and repair it after injury. The airway is surrounded by cartilage ventrolaterally and smooth muscle dorsally. Lineage tracing using Krt5-CreER shows dorsal BCs ... Full text Link to item Cite

Introduction

Journal Article ERS Monograph · January 1, 2021 Full text Cite

Lung organoids: powerful tools for studying lung stem cells and diseases

Journal Article ERS Monograph · January 1, 2021 Organoids are self-organising, three-dimensional structures derived from stem/progenitor cells grown in culture. They maintain, at least in part, cellular and molecular properties and physiological functions of embryonic or adult tissues. Organoids are pow ... Full text Cite

The next 10 years in lung stem cell research

Journal Article ERS Monograph · January 1, 2021 The preceding chapters have documented many of the exciting advances over the past decade in the burgeoning field of lung stem cell biology. We have seen how stem cells contribute to the construction and maintenance of the architecture and cellular composi ... Full text Cite

The alveolar stem cell niche of the mammalian lung

Chapter · January 1, 2020 The alveolar region of the mammalian lung evolved to enable highly efficient gas exchange between the millions of air-filled sacs known as alveoli and blood circulating through the pulmonary vessels. Each alveolus is composed of epithelial and mesenchymal ... Full text Cite

Human organoids: a new dimension in cell biology.

Journal Article Mol Biol Cell · May 1, 2019 Organoids derived from stem cells or tissues in culture can develop into structures that resemble the in vivo anatomy and physiology of intact organs. Human organoid cultures provide the potential to study human development and model disease processes with ... Full text Link to item Cite

IL-1 and TNFα Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration.

Journal Article Stem Cell Reports · April 9, 2019 Inflammatory responses are known to facilitate tissue recovery following injury. However, the precise mechanisms that enhance lung alveolar regeneration remain unclear. Here, using an organoid-based screening assay, we find that interleukin-1 (IL-1) and tu ... Full text Link to item Cite

Ager-CreERT2: A New Genetic Tool for Studying Lung Alveolar Development, Homeostasis, and Repair.

Journal Article Am J Respir Cell Mol Biol · December 2018 The alveolar region of the lung is composed of two major epithelial cell types: cuboidal alveolar type 2 cells (AT2 cells), which produce surfactant proteins, and large, thin, alveolar type 1 cells (AT1 cells), specialized for efficient gas exchange. AT1 c ... Full text Link to item Cite

The endoderm from a diverse perspective.

Journal Article Development · August 16, 2018 The historic town of Taos, New Mexico, with its rich multicultural history of art and craft, was the site of the second Keystone Symposium on 'Endoderm Development and Disease', which was held in February 2018. The theme of the meeting was 'Cross-Organ Com ... Full text Link to item Cite

Stemming Lung Disease?

Journal Article N Engl J Med · June 21, 2018 Full text Link to item Cite

Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation.

Journal Article Development · May 11, 2018 The bone morphogenetic protein (BMP) signaling pathway, including antagonists, functions in lung development and regeneration of tracheal epithelium from basal stem cells. Here, we explore its role in the alveolar region, where type 2 epithelial cells (AT2 ... Full text Link to item Cite

Integrating Mechanical Force into Lung Development.

Journal Article Dev Cell · February 5, 2018 In this issue of Developmental Cell, Tang et al. (2018) and Li et al. (2018) combine genetic manipulation, mechanical perturbation, and live imaging to show how mechanical forces and local growth factors intersect to influence epithelial behavior and cell ... Full text Link to item Cite

STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo.

Journal Article Proc Natl Acad Sci U S A · December 19, 2017 Regulated mesoderm migration is necessary for the proper morphogenesis and organ formation during embryonic development. Cell migration and its dependence on the cytoskeleton and signaling machines have been studied extensively in cultured cells; in contra ... Full text Link to item Cite

Lung organoids: current uses and future promise.

Journal Article Development · March 15, 2017 Lungs are composed of a system of highly branched tubes that bring air into the alveoli, where gas exchange takes place. The proximal and distal regions of the lung contain epithelial cells specialized for different functions: basal, secretory and ciliated ... Full text Link to item Cite

BMP signaling and cellular dynamics during regeneration of airway epithelium from basal progenitors.

Journal Article Development · March 1, 2016 The pseudostratified epithelium of the lung contains ciliated and secretory luminal cells and basal stem/progenitor cells. To identify signals controlling basal cell behavior we screened factors that alter their self-renewal and differentiation in a clonal ... Full text Link to item Cite

GRHL2 coordinates regeneration of a polarized mucociliary epithelium from basal stem cells.

Journal Article J Cell Biol · November 9, 2015 Pseudostratified airway epithelium of the lung is composed of polarized ciliated and secretory cells maintained by basal stem/progenitor cells. An important question is how lineage choice and differentiation are coordinated with apical-basal polarity and e ... Full text Link to item Cite

Telomere dysfunction causes alveolar stem cell failure.

Journal Article Proc Natl Acad Sci U S A · April 21, 2015 Telomere syndromes have their most common manifestation in lung disease that is recognized as idiopathic pulmonary fibrosis and emphysema. In both conditions, there is loss of alveolar integrity, but the underlying mechanisms are not known. We tested the c ... Full text Link to item Cite

Dorso-ventral heterogeneity in tracheal basal stem cells.

Journal Article Biol Open · September 15, 2021 The tracheal basal cells (BCs) function as stem cells to maintain the epithelium in steady state and repair it after injury. The airway is surrounded by cartilage ventrolaterally and smooth muscle dorsally. Lineage tracing using Krt5-CreER shows dorsal BCs ... Full text Link to item Cite

Introduction

Journal Article ERS Monograph · January 1, 2021 Full text Cite

Lung organoids: powerful tools for studying lung stem cells and diseases

Journal Article ERS Monograph · January 1, 2021 Organoids are self-organising, three-dimensional structures derived from stem/progenitor cells grown in culture. They maintain, at least in part, cellular and molecular properties and physiological functions of embryonic or adult tissues. Organoids are pow ... Full text Cite

The next 10 years in lung stem cell research

Journal Article ERS Monograph · January 1, 2021 The preceding chapters have documented many of the exciting advances over the past decade in the burgeoning field of lung stem cell biology. We have seen how stem cells contribute to the construction and maintenance of the architecture and cellular composi ... Full text Cite

The alveolar stem cell niche of the mammalian lung

Chapter · January 1, 2020 The alveolar region of the mammalian lung evolved to enable highly efficient gas exchange between the millions of air-filled sacs known as alveoli and blood circulating through the pulmonary vessels. Each alveolus is composed of epithelial and mesenchymal ... Full text Cite

Human organoids: a new dimension in cell biology.

Journal Article Mol Biol Cell · May 1, 2019 Organoids derived from stem cells or tissues in culture can develop into structures that resemble the in vivo anatomy and physiology of intact organs. Human organoid cultures provide the potential to study human development and model disease processes with ... Full text Link to item Cite

IL-1 and TNFα Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration.

Journal Article Stem Cell Reports · April 9, 2019 Inflammatory responses are known to facilitate tissue recovery following injury. However, the precise mechanisms that enhance lung alveolar regeneration remain unclear. Here, using an organoid-based screening assay, we find that interleukin-1 (IL-1) and tu ... Full text Link to item Cite

Ager-CreERT2: A New Genetic Tool for Studying Lung Alveolar Development, Homeostasis, and Repair.

Journal Article Am J Respir Cell Mol Biol · December 2018 The alveolar region of the lung is composed of two major epithelial cell types: cuboidal alveolar type 2 cells (AT2 cells), which produce surfactant proteins, and large, thin, alveolar type 1 cells (AT1 cells), specialized for efficient gas exchange. AT1 c ... Full text Link to item Cite

The endoderm from a diverse perspective.

Journal Article Development · August 16, 2018 The historic town of Taos, New Mexico, with its rich multicultural history of art and craft, was the site of the second Keystone Symposium on 'Endoderm Development and Disease', which was held in February 2018. The theme of the meeting was 'Cross-Organ Com ... Full text Link to item Cite

Stemming Lung Disease?

Journal Article N Engl J Med · June 21, 2018 Full text Link to item Cite

Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation.

Journal Article Development · May 11, 2018 The bone morphogenetic protein (BMP) signaling pathway, including antagonists, functions in lung development and regeneration of tracheal epithelium from basal stem cells. Here, we explore its role in the alveolar region, where type 2 epithelial cells (AT2 ... Full text Link to item Cite

Integrating Mechanical Force into Lung Development.

Journal Article Dev Cell · February 5, 2018 In this issue of Developmental Cell, Tang et al. (2018) and Li et al. (2018) combine genetic manipulation, mechanical perturbation, and live imaging to show how mechanical forces and local growth factors intersect to influence epithelial behavior and cell ... Full text Link to item Cite

STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo.

Journal Article Proc Natl Acad Sci U S A · December 19, 2017 Regulated mesoderm migration is necessary for the proper morphogenesis and organ formation during embryonic development. Cell migration and its dependence on the cytoskeleton and signaling machines have been studied extensively in cultured cells; in contra ... Full text Link to item Cite

Lung organoids: current uses and future promise.

Journal Article Development · March 15, 2017 Lungs are composed of a system of highly branched tubes that bring air into the alveoli, where gas exchange takes place. The proximal and distal regions of the lung contain epithelial cells specialized for different functions: basal, secretory and ciliated ... Full text Link to item Cite

BMP signaling and cellular dynamics during regeneration of airway epithelium from basal progenitors.

Journal Article Development · March 1, 2016 The pseudostratified epithelium of the lung contains ciliated and secretory luminal cells and basal stem/progenitor cells. To identify signals controlling basal cell behavior we screened factors that alter their self-renewal and differentiation in a clonal ... Full text Link to item Cite

GRHL2 coordinates regeneration of a polarized mucociliary epithelium from basal stem cells.

Journal Article J Cell Biol · November 9, 2015 Pseudostratified airway epithelium of the lung is composed of polarized ciliated and secretory cells maintained by basal stem/progenitor cells. An important question is how lineage choice and differentiation are coordinated with apical-basal polarity and e ... Full text Link to item Cite

Telomere dysfunction causes alveolar stem cell failure.

Journal Article Proc Natl Acad Sci U S A · April 21, 2015 Telomere syndromes have their most common manifestation in lung disease that is recognized as idiopathic pulmonary fibrosis and emphysema. In both conditions, there is loss of alveolar integrity, but the underlying mechanisms are not known. We tested the c ... Full text Link to item Cite

Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung.

Journal Article Nat Commun · April 13, 2015 The plasticity of differentiated cells in adult tissues undergoing repair is an area of intense research. Pulmonary alveolar type II cells produce surfactant and function as progenitors in the adult, demonstrating both self-renewal and differentiation into ... Full text Open Access Link to item Cite

Development of the Respiratory System

Chapter · January 1, 2015 Lung development requires the coordinated growth and morphogenesis of multiple populations: the endodermal epithelium of the anterior foregut, splanchic mesoderm, mesothelium, vasculature and lymphatics, and nerves. Together they give rise to the respirato ... Full text Cite

IL-6/STAT3 promotes regeneration of airway ciliated cells from basal stem cells.

Journal Article Proc Natl Acad Sci U S A · September 2, 2014 The pseudostratified airway epithelium of the lung contains a balanced proportion of multiciliated and secretory luminal cells that are maintained and regenerated by a population of basal stem cells. However, little is known about how these processes are m ... Full text Link to item Cite

The cell of origin and subtype of K-Ras-induced lung tumors are modified by Notch and Sox2.

Journal Article Genes Dev · September 1, 2014 Cell type-specific conditional activation of oncogenic K-Ras is a powerful tool for investigating the cell of origin of adenocarcinomas in the mouse lung. Our previous studies showed that K-Ras activation with a CC10(Scgb1a1)-CreER driver leads to adenocar ... Full text Link to item Cite

Repair and regeneration of the respiratory system: complexity, plasticity, and mechanisms of lung stem cell function.

Journal Article Cell Stem Cell · August 7, 2014 Respiratory disease is the third leading cause of death in the industrialized world. Consequently, the trachea, lungs, and cardiopulmonary vasculature have been the focus of extensive investigations. Recent studies have provided new information about the m ... Full text Link to item Cite

Age-related changes in the cellular composition and epithelial organization of the mouse trachea.

Journal Article PLoS One · 2014 We report here senescent changes in the structure and organization of the mucociliary pseudostratified epithelium of the mouse trachea and main stem bronchi. We confirm previous reports of the gradual appearance of age-related, gland-like structures (ARGLS ... Full text Open Access Link to item Cite

Loss of basal cells precedes bronchiolitis obliterans-like pathological changes in a murine model of chlorine gas inhalation.

Journal Article Am J Respir Cell Mol Biol · November 2013 Bronchiolitis obliterans (BO) is a major cause of chronic airway dysfunction after toxic chemical inhalation. The pathophysiology of BO is not well understood, but epithelial cell injury has been closely associated with the development of fibrotic lesions ... Full text Link to item Cite

Type 2 alveolar cells are stem cells in adult lung.

Journal Article J Clin Invest · July 2013 Gas exchange in the lung occurs within alveoli, air-filled sacs composed of type 2 and type 1 epithelial cells (AEC2s and AEC1s), capillaries, and various resident mesenchymal cells. Here, we use a combination of in vivo clonal lineage analysis, different ... Full text Open Access Link to item Cite

Evidence for multiple roles for grainyhead-like 2 in the establishment and maintenance of human mucociliary airway epithelium.[corrected].

Journal Article Proc Natl Acad Sci U S A · June 4, 2013 Most of the airways of the human lung are lined by an epithelium made up of ciliated and secretory luminal cells and undifferentiated basal progenitor cells. The integrity of this epithelium and its ability to act as a selective barrier are critical for no ... Full text Link to item Cite

Stem cells of the adult lung: their development and role in homeostasis, regeneration, and disease.

Journal Article Wiley Interdiscip Rev Dev Biol · 2013 The lung has vital functions in gas exchange and immune defense. To fulfill these functions the cellular composition and complex three-dimensional organization of the organ must be maintained for a lifetime. Cell turnover in the adult lung is normally low. ... Full text Link to item Cite

Calcium-activated chloride channel TMEM16A modulates mucin secretion and airway smooth muscle contraction.

Journal Article Proc Natl Acad Sci U S A · October 2, 2012 Mucous cell hyperplasia and airway smooth muscle (ASM) hyperresponsiveness are hallmark features of inflammatory airway diseases, including asthma. Here, we show that the recently identified calcium-activated chloride channel (CaCC) TMEM16A is expressed in ... Full text Link to item Cite

Evidence for type II cells as cells of origin of K-Ras-induced distal lung adenocarcinoma.

Journal Article Proc Natl Acad Sci U S A · March 27, 2012 Identifying the cells of origin of lung cancer may lead to new therapeutic strategies. Previous work has focused upon the putative bronchoalveolar stem cell at the bronchioalveolar duct junction as a cancer cell of origin when a codon 12 K-Ras mutant is in ... Full text Link to item Cite

Multiple stromal populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition.

Journal Article Proc Natl Acad Sci U S A · December 27, 2011 There are currently few treatment options for pulmonary fibrosis. Innovations may come from a better understanding of the cellular origin of the characteristic fibrotic lesions. We have analyzed normal and fibrotic mouse and human lungs by confocal microsc ... Full text Link to item Cite

Notch-dependent differentiation of adult airway basal stem cells.

Journal Article Cell Stem Cell · June 3, 2011 The epithelium lining the airways of the adult human lung is composed of ciliated and secretory cells together with undifferentiated basal cells (BCs). The composition and organization of this epithelium is severely disrupted in many respiratory diseases. ... Full text Link to item Cite

Cell plasticity in lung injury and repair: report from an NHLBI workshop, April 19-20, 2010.

Journal Article Proc Am Thorac Soc · June 2011 In April 2010, a NIH workshop was convened to discuss the current state of understanding of lung cell plasticity, including the responses of epithelial cells to injury, with the objectives of summarizing what is known, what the field needs to know, and how ... Full text Link to item Cite

Role of E-cadherin in the pathogenesis of gastroesophageal reflux disease.

Journal Article Am J Gastroenterol · June 2011 OBJECTIVES: An early event in the pathogenesis of gastroesophageal reflux disease (GERD) is an acid-induced increase in junctional (paracellular) permeability in esophageal epithelium (EE). The molecular events that account for this change are unknown. E-c ... Full text Link to item Cite

Transcriptome analysis of mouse stem cells and early embryos

Chapter · January 1, 2011 Understanding and harnessing cellular potency are fundamental in biology and are also critical to the future therapeutic use of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such goals. Starting with sources that inc ... Cite

Epithelial progenitor cells in lung development, maintenance, repair, and disease.

Journal Article Annu Rev Cell Dev Biol · 2011 The vertebrate lung is elegantly patterned to carry out gas exchange and host defense. Similar to other organ systems, endogenous stem and progenitor cells fuel the organogenesis of the lung and maintain homeostasis in the face of normal wear and tear. In ... Full text Link to item Cite

Lineage Tracing Of Mature Type 2 Alveolar Epithelial Cells Reveals New Insights Into Alveolar Maintenance And Repair

Journal Article AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2011 Link to item Cite

BMP signaling in the development of the mouse esophagus and forestomach.

Journal Article Development · December 2010 The stratification and differentiation of the epidermis are known to involve the precise control of multiple signaling pathways. By contrast, little is known about the development of the mouse esophagus and forestomach, which are composed of a stratified s ... Full text Open Access Link to item Cite

Developmental biology. Branching takes nerve.

Journal Article Science · September 24, 2010 Full text Link to item Cite

Evidence that SOX2 overexpression is oncogenic in the lung.

Journal Article PLoS One · June 10, 2010 BACKGROUND: SOX2 (Sry-box 2) is required to maintain a variety of stem cells, is overexpressed in some solid tumors, and is expressed in epithelial cells of the lung. METHODOLOGY/PRINCIPAL FINDINGS: We show that SOX2 is overexpressed in human squamous cell ... Full text Open Access Link to item Cite

Preparing for the first breath: genetic and cellular mechanisms in lung development.

Journal Article Dev Cell · January 19, 2010 The mammalian respiratory system--the trachea and the lungs--arises from the anterior foregut through a sequence of morphogenetic events involving reciprocal endodermal-mesodermal interactions. The lung itself consists of two highly branched, tree-like sys ... Full text Link to item Cite

Airway basal stem cells: a perspective on their roles in epithelial homeostasis and remodeling.

Journal Article Dis Model Mech · 2010 The small airways of the human lung undergo pathological changes in pulmonary disorders, such as chronic obstructive pulmonary disease (COPD), asthma, bronchiolitis obliterans and cystic fibrosis. These clinical problems impose huge personal and societal h ... Full text Open Access Link to item Cite

Essentials of Stem Cell Biology

Journal Article Essentials of Stem Cell Biology · December 1, 2009 First developed as an accessible abridgement of the successful Handbook of Stem Cells, Essentials of Stem Cell Biology serves the needs of the evolving population of scientists, researchers, practitioners and students that are embracing the latest advances ... Cite

The Id2+ distal tip lung epithelium contains individual multipotent embryonic progenitor cells.

Journal Article Development · November 2009 The conducting airways (bronchi and bronchioles) and peripheral gas exchange (alveolar) regions of the mammalian lung are generated by a process of branching morphogenesis. Evidence suggests that during embryonic development, the undifferentiated epithelia ... Full text Link to item Cite

Basal cells as stem cells of the mouse trachea and human airway epithelium.

Journal Article Proc Natl Acad Sci U S A · August 4, 2009 The pseudostratified epithelium of the mouse trachea and human airways contains a population of basal cells expressing Trp-63 (p63) and cytokeratins 5 (Krt5) and Krt14. Using a KRT5-CreER(T2) transgenic mouse line for lineage tracing, we show that basal ce ... Full text Link to item Cite

The role of Scgb1a1+ Clara cells in the long-term maintenance and repair of lung airway, but not alveolar, epithelium.

Journal Article Cell Stem Cell · June 5, 2009 To directly test the contribution of Scgb1a1(+) Clara cells to postnatal growth, homeostasis, and repair of lung epithelium, we generated a Scgb1a1-CreER "knockin" mouse for lineage-tracing these cells. Under all conditions tested, the majority of Clara ce ... Full text Link to item Cite

Multiple roles for Sox2 in the developing and adult mouse trachea.

Journal Article Development · June 2009 The esophagus, trachea and lung develop from the embryonic foregut, yet acquire and maintain distinct tissue phenotypes. Previously, we demonstrated that the transcription factor Sox2 is necessary for foregut morphogenesis and esophagus development. We sho ... Full text Link to item Cite

Cell lineage mapping of taste bud cells and keratinocytes in the mouse tongue and soft palate.

Journal Article Stem Cells · February 2009 The epithelium of the mouse tongue and soft palate consists of at least three distinct epithelial cell populations: basal cells, keratinized cells organized into filiform and fungiform papillae, and taste receptor cells present in tight clusters known as t ... Full text Link to item Cite

Essentials of Stem Cell Biology, Second Edition

Book · January 1, 2009 First developed as an accessible abridgement of the successful Handbook of Stem Cells, Essentials of Stem Cell Biology serves the needs of the evolving population of scientists, researchers, practitioners and students that are embracing the latest advances ... Full text Cite

Culture of endodermal stem/progenitor cells of the mouse tongue.

Journal Article In Vitro Cell Dev Biol Anim · 2009 The tongue represents a very accessible source of tissue-specific epithelial stem cells of endodermal origin. However, little is known about the properties of these cells and the mechanisms regulating their proliferation and differentiation. Foxa2, an endo ... Full text Link to item Cite

Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development.

Journal Article Proc Natl Acad Sci U S A · October 28, 2008 During mouse development, the sophisticated vascular network of the lung is established from embryonic day (E) approximately 10.5 and continues to develop postnatally. This network is composed of endothelial cells enclosed by vascular smooth muscle, pericy ... Full text Link to item Cite

Basal cells in lung cancer

Conference Journal of the American College of Surgeons · September 2008 Full text Cite

Ciliated epithelial cell lifespan in the mouse trachea and lung.

Journal Article Am J Physiol Lung Cell Mol Physiol · July 2008 The steady-state turnover of epithelial cells in the lung and trachea is highly relevant to investigators who are studying endogenous stem cells, manipulating gene expression in vivo, or using viral vectors for gene therapy. However, the average lifetime o ... Full text Link to item Cite

Epithelial progenitor cells of the embryonic lung and the role of microRNAs in their proliferation.

Journal Article Proc Am Thorac Soc · April 15, 2008 The entire epithelium of the lung is generated from a small pool of undifferentiated progenitor cells. At least during the early stages of development these reside in the distal tips of the embryonic lung. They respond to multiple signals from the surround ... Full text Link to item Cite

Bmp4 is essential for the formation of the vestibular apparatus that detects angular head movements.

Journal Article PLoS Genet · April 11, 2008 Angular head movements in vertebrates are detected by the three semicircular canals of the inner ear and their associated sensory tissues, the cristae. Bone morphogenetic protein 4 (Bmp4), a member of the Transforming growth factor family (TGF-beta), is co ... Full text Link to item Cite

Endothelial Bmp4 is induced during arterial remodeling: effects on smooth muscle cell migration and proliferation.

Journal Article J Surg Res · March 2008 BACKGROUND: Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-beta superfamily of proteins that have multiple functional roles in mammalian development. A role for BMP4 in adult vascular remodeling has recently been suggested ... Full text Link to item Cite

Epithelial stem/progenitor cells in lung postnatal growth, maintenance, and repair.

Journal Article Cold Spring Harb Symp Quant Biol · 2008 The adult lung consists of a trachea leading into a system of branched airways ending in millions of alveolar sacs. It contains many different epithelial cell types arranged in precise patterns along the proximodistal axis. Each region of the lung has the ... Full text Link to item Cite

Summary: present and future challenges for stem cell research.

Journal Article Cold Spring Harb Symp Quant Biol · 2008 Stem cell research is being driven forward at an intense pace by creative interactions among scientists working in different fields. These include developmental and reproductive biology, regeneration, genomics, live cell imaging, RNA biology, and cancer bi ... Full text Link to item Cite

A shared vision.

Journal Article Dev Cell · December 2007 One of today's most powerful technologies in biomedical research--the creation of mutant mice by gene targeting in embryonic stem (ES) cells--was finally celebrated in this year's Nobel Prize in Medicine. The history of how ES cells were first discovered a ... Full text Link to item Cite

Transgenic over-expression of the microRNA miR-17-92 cluster promotes proliferation and inhibits differentiation of lung epithelial progenitor cells.

Journal Article Dev Biol · October 15, 2007 The miR-17-92 locus encodes a cluster of 7 microRNAs transcribed as a single primary transcript. It can accelerate c-Myc induced B cell lymphoma development and is highly expressed in many tumors, including lung tumors. However, the role of miR-17-92 in de ... Full text Link to item Cite

Ankyrin-G is a molecular partner of E-cadherin in epithelial cells and early embryos.

Journal Article J Biol Chem · September 7, 2007 E-cadherin is a ubiquitous component of lateral membranes in epithelial tissues and is required to form the first lateral membrane domains in development. Here, we identify ankyrin-G as a molecular partner of E-cadherin and demonstrate that ankyrin-G and b ... Full text Link to item Cite

Retrospective. Anne McLaren (1927-2007).

Journal Article Science · August 3, 2007 Full text Link to item Cite

Global gene expression profiling reveals similarities and differences among mouse pluripotent stem cells of different origins and strains.

Journal Article Dev Biol · July 15, 2007 Pluripotent stem cell lines with similar phenotypes can be derived from both blastocysts (embryonic stem cells, ESC) and primordial germ cells (embryonic germ cells, EGC). Here, we present a compendium DNA microarray analysis of multiple mouse ESCs and EGC ... Full text Link to item Cite

Multiple dose-dependent roles for Sox2 in the patterning and differentiation of anterior foregut endoderm.

Journal Article Development · July 2007 Sox2 is expressed in developing foregut endoderm, with highest levels in the future esophagus and anterior stomach. By contrast, Nkx2.1 (Titf1) is expressed ventrally, in the future trachea. In humans, heterozygosity for SOX2 is associated with anopthalmia ... Full text Link to item Cite

Lung development and repair: contribution of the ciliated lineage.

Journal Article Proc Natl Acad Sci U S A · January 9, 2007 The identity of the endogenous epithelial cells in the adult lung that are responsible for normal turnover and repair after injury is still controversial. In part, this is due to a paucity of highly specific genetic lineage tools to follow efficiently the ... Full text Link to item Cite

Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes.

Journal Article Mol Syst Biol · 2007 Although microarray analysis has provided information regarding the dynamics of gene expression during development of the mouse lung, no extensive correlations have been made to the levels of corresponding protein products. Here, we present a global survey ... Full text Link to item Cite

Sox2 is required for development of taste bud sensory cells.

Journal Article Genes Dev · October 1, 2006 Sox2 is expressed in basal epithelial cells of the tongue, with high levels in taste bud placodes, fungiform papillae, and mature taste cells, and low levels in filiform papillae. High Sox2 expression appears to lie downstream from canonical Wnt signaling. ... Full text Link to item Cite

Building organs from buds, branches and tubes.

Journal Article Differentiation · September 2006 Full text Link to item Cite

Morphogenesis of the trachea and esophagus: current players and new roles for noggin and Bmps.

Journal Article Differentiation · September 2006 The development of the anterior foregut of the mammalian embryo involves changes in the behavior of both the epithelial endoderm and the adjacent mesoderm. Morphogenetic processes that occur include the extrusion of midline notochord cells from the epithel ... Full text Link to item Cite

From the authors [4]

Journal Article American Journal of Respiratory and Critical Care Medicine · August 1, 2006 Full text Cite

Against the ATS statement on human embryonic stem cell research - Reply

Journal Article AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · August 1, 2006 Link to item Cite

Epithelial stem cells of the lung: privileged few or opportunities for many?

Journal Article Development · July 2006 Most reviews of adult stem cells focus on the relatively undifferentiated cells dedicated to the renewal of rapidly proliferating tissues, such as the skin, gut and blood. By contrast, there is mounting evidence that organs and tissues such as the liver an ... Full text Link to item Cite

Bmp signaling is required for intestinal growth and morphogenesis.

Journal Article Dev Dyn · June 2006 Intestinal growth, morphogenesis, differentiation, and homeostasis are regulated by reciprocal interactions between the epithelium and the underlying mesenchymal stroma. The identification of BMPR1A mutations in patients with Juvenile Polyposis implicates ... Full text Link to item Cite

Identification of Tgf beta1i4 as a downstream target of Foxc1.

Journal Article Dev Growth Differ · June 2006 Craniofacial development is severely affected by null mutations in Foxc1, indicating a multifunctional role for Foxc1 in ocular, maxilla and mandible, skull and facial gland development. To delineate signaling pathways in which Foxc1 is involved we compare ... Full text Link to item Cite

The role of the forkhead transcription factor, Foxc1, in the development of the mouse lacrimal gland.

Journal Article Dev Dyn · April 2006 The lacrimal gland produces secretions that lubricate and protect the cornea of the eye. Foxc1 encodes a forkhead/winged helix transcription factor required for the development of many embryonic organs. Autosomal dominant mutations in human FOXC1 cause eye ... Full text Link to item Cite

CHMP5 is essential for late endosome function and down-regulation of receptor signaling during mouse embryogenesis.

Journal Article J Cell Biol · March 27, 2006 Charged MVB protein 5 (CHMP5) is a coiled coil protein homologous to the yeast Vps60/Mos10 gene and other ESCRT-III complex members, although its precise function in either yeast or mammalian cells is unknown. We deleted the CHMP5 gene in mice, resulting i ... Full text Link to item Cite

Evidence that autocrine signaling through Bmpr1a regulates the proliferation, survival and morphogenetic behavior of distal lung epithelial cells.

Journal Article Dev Biol · March 1, 2006 Lung development requires reciprocal epithelial/mesenchymal interactions, mediated by signaling factors such as Bmps made in both cell populations. To address the role of Bmp signaling in the epithelium, we have exploited the fact that Bmp receptor type Ia ... Full text Link to item Cite

The mouse forkhead gene Foxc1 is required for primordial germ cell migration and antral follicle development.

Journal Article Dev Biol · February 15, 2006 Foxc1 encodes a forkhead/winged helix transcription factor expressed in many embryonic tissues. Previous studies have investigated defects in the urogenital system of Foxc1 null mutants, but the mechanisms underlying the abnormal development of the gonad h ... Full text Link to item Cite

Intercellular growth factor signaling and the development of mouse tracheal submucosal glands.

Journal Article Dev Dyn · August 2005 To provide a genetic framework for investigating changes in airway submucosal gland function in human respiratory disease, we have investigated their counterparts in normal and mutant mice. We describe their morphogenesis in relation to the expression of g ... Full text Link to item Cite

An essential role for an inositol polyphosphate multikinase, Ipk2, in mouse embryogenesis and second messenger production.

Journal Article Proc Natl Acad Sci U S A · June 14, 2005 Phospholipase C and several inositol polyphosphate kinase (IPK) activities generate a branched ensemble of inositol polyphosphate second messengers that regulate cellular signaling pathways in the nucleus and cytoplasm. Here, we report that mice deficient ... Full text Link to item Cite

Nmyc plays an essential role during lung development as a dosage-sensitive regulator of progenitor cell proliferation and differentiation.

Journal Article Development · March 2005 Understanding how lung progenitor cells balance proliferation against differentiation is relevant to clinical disorders such as bronchopulmonary dysplasia of premature babies and lung cancer. Previous studies have established that lung development is sever ... Full text Link to item Cite

Handbook of Stem Cells

Journal Article Handbook of Stem Cells · September 14, 2004 New discoveries in the field of stem cell research have frequently appeared in the news and in scientific literature. Research in this area promises to lead to new therapies for cancer, heart disease, diabetes, and a wide variety of other diseases. This tw ... Cite

The forkhead genes, Foxc1 and Foxc2, regulate paraxial versus intermediate mesoderm cell fate.

Journal Article Dev Biol · July 1, 2004 During vertebrate embryogenesis, the newly formed mesoderm is allocated to the paraxial, intermediate, and lateral domains, each giving rise to different cell and tissue types. Here, we provide evidence that the forkhead genes, Foxc1 and Foxc2, play a role ... Full text Link to item Cite

Deconstructing the genesis of animal form.

Journal Article Development · June 2004 Santa Fe - with its museums and galleries full of art and crafts inspired by natural forms - was the perfect setting for a Keystone conference on vertebrate organogenesis in February 2004. Organized by Gail Martin and Cliff Tabin, the conference sessions w ... Full text Link to item Cite

Novel role for Netrins in regulating epithelial behavior during lung branching morphogenesis.

Journal Article Curr Biol · May 25, 2004 The development of many organs, including the lung, depends upon a process known as branching morphogenesis, in which a simple epithelial bud gives rise to a complex tree-like system of tubes specialized for the transport of gas or fluids. Previous studies ... Full text Link to item Cite

Hyperactive Wnt signaling changes the developmental potential of embryonic lung endoderm.

Journal Article J Biol · 2004 BACKGROUND: Studies in many model systems have shown that canonical signaling through the pathway downstream of ligands of the Wnt family can regulate multiple steps in organogenesis, including cell proliferation, differentiation, and lineage specification ... Full text Link to item Cite

Transcriptome analysis of mouse stem cells and early embryos.

Journal Article PLoS Biol · December 2003 Understanding and harnessing cellular potency are fundamental in biology and are also critical to the future therapeutic use of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such goals. Starting with sources that inc ... Full text Link to item Cite

Ecsit is required for Bmp signaling and mesoderm formation during mouse embryogenesis.

Journal Article Genes Dev · December 1, 2003 Bone morphogenetic proteins (Bmps) are members of the transforming growth factor beta (TGFbeta) superfamily that play critical roles during mouse embryogenesis. Signaling by Bmp receptors is mediated mainly by Smad proteins. In this study, we show that a t ... Full text Link to item Cite

An essential role of Bmp4 in the atrioventricular septation of the mouse heart.

Journal Article Genes Dev · October 1, 2003 Proper septation and valvulogenesis during cardiogenesis depend on interactions between the myocardium and the endocardium. By combining use of a hypomorphic Bone morphogenetic protein 4 (Bmp4) allele with conditional gene inactivation, we here identify Bm ... Full text Link to item Cite

Role for ETS domain transcription factors Pea3/Erm in mouse lung development.

Journal Article Dev Biol · September 1, 2003 During the development of the mouse lung, the expression of a number of genes, including those encoding growth factors and components of their downstream signaling pathways, is enriched in the epithelium and/or mesenchyme of the distal buds. In this locati ... Full text Link to item Cite

Tissue interactions pattern the mesenchyme of the embryonic mouse lung.

Journal Article Dev Biol · June 1, 2003 The mechanisms that control proliferation and differentiation of embryonic lung mesenchyme are largely unknown. We describe an explant system in which exogenous recombinant N-Sonic Hedgehog (N-Shh) protein sustains the survival and proliferation of lung me ... Full text Link to item Cite

Developmentally regulated expression of two members of the Nrarp family in zebrafish.

Journal Article Gene Expr Patterns · May 2003 Delta-Notch signaling is essential for somitogenesis in vertebrate embryos. In a search for genes that control somite formation in zebrafish we have identified two paralogues encoding proteins related to Nrarp (Notch regulated ankyrin repeat protein). Zebr ... Full text Link to item Cite

BMP ligands act redundantly to pattern the dorsal telencephalic midline.

Journal Article Genesis · April 2003 The embryonic telencephalon is patterned into several areas that give rise to functionally distinct structures in the adult forebrain. Previous studies have shown that BMP4 and BMP2 can induce features characteristic of the telencephalic midline in culture ... Full text Link to item Cite

Differential gene expression in the distal tip endoderm of the embryonic mouse lung.

Journal Article Gene Expr Patterns · December 2002 During the early development of the mouse lung a number of genes encoding signaling molecules are differentially expressed in the epithelium and mesenchyme of the distal buds. Evidence suggests they play a role in regulating the stereotypic processes of bu ... Full text Link to item Cite

Identification of mZnf8, a mouse Krüppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1.

Journal Article Mol Cell Biol · November 2002 To identify novel genes that play critical roles in mediating bone morphogenetic protein (BMP) signal pathways, we performed a yeast two-hybrid screen using Smad1 as bait. A novel mouse Krüppel-type zinc finger protein, mZnf8, was isolated. Interactions be ... Full text Link to item Cite

Distinct requirements for extra-embryonic and embryonic bone morphogenetic protein 4 in the formation of the node and primitive streak and coordination of left-right asymmetry in the mouse.

Journal Article Development · October 2002 Featured Publication In the mouse and chick embryo, the node plays a central role in generating left-right (LR) positional information. Using several different strategies, we provide evidence in the mouse that bone morphogenetic protein 4 (Bmp4) is required independently in tw ... Full text Link to item Cite

Organogenesis: molecular mechanisms of tubulogenesis.

Journal Article Nat Rev Genet · July 2002 Featured Publication As organisms have evolved in size and complexity, tubular systems have developed to enable the efficient transport of substances into and out of tissues. These tubular systems are generated using strategies that are based on common elements of cell behavio ... Full text Link to item Cite

Indian hedgehog as a progesterone-responsive factor mediating epithelial-mesenchymal interactions in the mouse uterus.

Journal Article Dev Biol · May 15, 2002 Genes encoding components of the hedgehog signaling pathway are dynamically expressed in the mouse uterus preparing for implantation. Indian hedgehog (Ihh), patched (Ptc), and Gli3 are expressed at low levels in the endometrial epithelium on day 1 of pregn ... Full text Link to item Cite

Bone morphogenetic protein 4 in the extraembryonic mesoderm is required for allantois development and the localization and survival of primordial germ cells in the mouse.

Journal Article Proc Natl Acad Sci U S A · November 20, 2001 Evidence suggests that the specification of primordial germ cells (PGCs) in the mammalian embryo does not depend on maternal determinants. Rather, previous genetic analysis in the mouse has shown that bone morphogenetic protein 4 (Bmp4) is required for the ... Full text Link to item Cite

The winged helix transcription factor Foxc1a is essential for somitogenesis in zebrafish.

Journal Article Genes Dev · September 15, 2001 Previous studies identified zebrafish foxc1a and foxc1b as homologs of the mouse forkhead gene, Foxc1. Both genes are transcribed in the unsegmented presomitic mesoderm (PSM), newly formed somites, adaxial cells, and head mesoderm. Here, we show that inhib ... Full text Link to item Cite

The murine winged helix transcription factors, Foxc1 and Foxc2, are both required for cardiovascular development and somitogenesis.

Journal Article Genes Dev · September 15, 2001 Featured Publication The murine Foxc1/Mf1 and Foxc2/Mfh1 genes encode closely related forkhead/winged helix transcription factors with overlapping expression in the forming somites and head mesoderm and endothelial and mesenchymal cells of the developing heart and blood vessel ... Full text Link to item Cite

Distinct mesodermal signals, including BMPs from the septum transversum mesenchyme, are required in combination for hepatogenesis from the endoderm.

Journal Article Genes Dev · August 1, 2001 Mesodermal signaling is critical for patterning the embryonic endoderm into different tissue domains. Classical tissue transplant experiments in the chick and recent studies in the mouse indicated that interactions with the cardiogenic mesoderm are necessa ... Full text Link to item Cite

Powerful ideas driven by simple tools: lessons from experimental embryology.

Journal Article Nat Cell Biol · July 2001 In developmental biology, as in all scientific fields, conceptual advances are tightly coupled to technological innovation. In this review, we trace the evolution of techniques in experimental embryology, from classical ablation to the latest methods utili ... Full text Link to item Cite

How does the mouse get its trunk?

Journal Article Nat Genet · April 2001 Full text Link to item Cite

Sequence and expression of zebrafish foxc1a and foxc1b, encoding conserved forkhead/winged helix transcription factors.

Journal Article Mech Dev · February 2001 Mouse Foxc1 (previously Mf1) is a member of the conserved forkhead/winged helix transcription factor gene family. It is expressed in many mesodermal tissues including paraxial mesoderm of the trunk and head, prechondrogenic mesenchyme, branchial arches and ... Full text Link to item Cite

Cellular and molecular responses of the uterus to embryo implantation can be elicited by locally applied growth factors.

Journal Article Proc Natl Acad Sci U S A · January 30, 2001 The implantation of a blastocyst into a receptive uterus is associated with a series of events, namely the attachment reaction followed by decidualization of the stroma. Previous studies established that the gene encoding heparin-binding EGF-like growth fa ... Full text Link to item Cite

Haploinsufficient Bmp4 ocular phenotypes include anterior segment dysgenesis with elevated intraocular pressure.

Journal Article BMC Genet · 2001 BACKGROUND: Glaucoma is a blinding disease usually associated with high intraocular pressure (IOP). In some families, abnormal anterior segment development contributes to glaucoma. The genes causing anterior segment dysgenesis and glaucoma in most of these ... Full text Link to item Cite

International Journal of Developmental Biology: Preface

Journal Article International Journal of Developmental Biology · January 1, 2001 Cite

Inhibition of Bmp signaling affects growth and differentiation in the anagen hair follicle.

Journal Article EMBO J · December 15, 2000 Growth and differentiation of postnatal hair follicles are controlled by reciprocal interactions between the dermal papilla and the surrounding epidermal hair precursors. The molecular nature of these interactions is largely unknown, but they are likely to ... Full text Link to item Cite

Notch/Delta expression in the developing mouse lung.

Journal Article Mech Dev · November 2000 Factors controlling the differentiation of the multipotent embryonic lung endoderm and mesoderm are poorly understood. Recent evidence that Delta-like 1 (Dll1) and other genes in the Notch/Delta signaling pathway are expressed in the embryonic mouse lung s ... Full text Link to item Cite

Development and validation of a liquid chromatographic method for the determination of the related substances of ramipril in Altace capsules.

Journal Article J Pharm Biomed Anal · September 2000 The development and validation of a reversed-phase liquid chromatographic method for the determination of the related substances of 2-[N-[(S)-1-Ethoxycarbonyl-3-phenylpropyl]-L-alanyl]-(1S, 3S, 5S)-2-azabicyclo[3.3.0]octane-3-carboxylic acid (ramipril) in ... Full text Link to item Cite

Bmp4 and Fgf10 play opposing roles during lung bud morphogenesis.

Journal Article Development · June 2000 Featured Publication Morphogenesis of the mouse lung involves reciprocal interactions between the epithelial endoderm and the surrounding mesenchyme, leading to an invariant early pattern of branching that forms the basis of the respiratory tree. There is evidence that Fibrobl ... Full text Link to item Cite

Haploinsufficiency of the transcription factors FOXC1 and FOXC2 results in aberrant ocular development.

Journal Article Hum Mol Genet · April 12, 2000 Anterior segment developmental disorders, including Axenfeld-Rieger anomaly (ARA), variably associate with harmfully elevated intraocular pressure (IOP), which causes glaucoma. Clinically observed dysgenesis does not correlate with IOP, however, and the et ... Full text Link to item Cite

Bone morphogenetic protein 4 regulates the budding site and elongation of the mouse ureter.

Journal Article J Clin Invest · April 2000 In the normal mouse embryo, Bmp4 is expressed in mesenchymal cells surrounding the Wolffian duct (WD) and ureter stalk, whereas bone morphogenetic protein (BMP) type I receptor genes are transcribed either ubiquitously (Alk3) or exclusively in the WD and u ... Full text Link to item Cite

Murine forkhead/winged helix genes Foxc1 (Mf1) and Foxc2 (Mfh1) are required for the early organogenesis of the kidney and urinary tract.

Journal Article Development · April 2000 The murine genes, Foxc1 and Foxc2 (previously, Mf1 and Mfh1), encode forkhead/winged helix transcription factors with virtually identical DNA-binding domains and overlapping expression patterns in various embryonic tissues. Foxc1/Mf1 is disrupted in the mu ... Full text Link to item Cite

Out of Eden: stem cells and their niches.

Journal Article Science · February 25, 2000 Stem cells are currently in the news for two reasons: the successful cultivation of human embryonic stem cell lines and reports that adult stem cells can differentiate into developmentally unrelated cell types, such as nerve cells into blood cells. Both in ... Full text Link to item Cite

Minimal phenotype of mice homozygous for a null mutation in the forkhead/winged helix gene, Mf2.

Journal Article Mol Cell Biol · February 2000 Mf2 (mesoderm/mesenchyme forkhead 2) encodes a forkhead/winged helix transcription factor expressed in numerous tissues of the mouse embryo, including paraxial mesoderm, somites, branchial arches, vibrissae, developing central nervous system, and developin ... Full text Link to item Cite

Attenuated host resistance against Mycobacterium bovis BCG infection in mice lacking osteopontin

Journal Article International Journal of Leprosy and Other Mycobacterial Diseases · December 1, 1999 Expression of the cytokine osteopontin (OPN) is elevated in granulomas caused by Mycobacterium tuberculosis. We tested the hypothesis that OPN contributes to host protection in a mouse model of mycobacterial infection. When infected with M. bovis BCG, mice ... Cite

Bone morphogenetic protein-6 is a marker of serous acinar cell differentiation in normal and neoplastic human salivary gland.

Journal Article Cancer Res · November 15, 1999 Bone morphogenetic protein (BMP-6, also known as vegetal-pale-gene-related and decaplentaplegic-vegetal-related) is a member of the transforming growth factor-beta superfamily of multifunctional signaling molecules. BMP-6 appears to play various biological ... Link to item Cite

Roles for the winged helix transcription factors MF1 and MFH1 in cardiovascular development revealed by nonallelic noncomplementation of null alleles.

Journal Article Dev Biol · September 15, 1999 The murine Mf1 and Mfh1 genes have overlapping patterns of expression in the embryo and encode forkhead/winged helix transcription factors with virtually identical DNA binding domains. Previous studies have shown that Mfh1 null mutants have severe cardiova ... Full text Link to item Cite

Bmp signaling regulates proximal-distal differentiation of endoderm in mouse lung development.

Journal Article Development · September 1999 Featured Publication In the mature mouse lung, the proximal-distal (P-D) axis is delineated by two distinct epithelial subpopulations: the proximal bronchiolar epithelium and the distal respiratory epithelium. Little is known about the signaling molecules that pattern the lung ... Full text Link to item Cite

Attenuated host resistance against Mycobacterium bovis BCG infection in mice lacking osteopontin.

Journal Article Infect Immun · August 1999 Expression of the cytokine osteopontin (OPN) is elevated in granulomas caused by Mycobacterium tuberculosis. We tested the hypothesis that OPN contributes to host protection in a mouse model of mycobacterial infection. When infected with Mycobacterium bovi ... Full text Link to item Cite

The forkhead/winged-helix gene, Mf1, is necessary for the normal development of the cornea and formation of the anterior chamber in the mouse eye.

Journal Article Dev Biol · July 15, 1999 Mf1, which encodes a winged-helix/forkhead transcription factor, is the murine homolog of human FKHL7, mutated in individuals with autosomal dominant inherited dysgenesis of the anterior segment of the eye (Axenfeld-Reiger anomaly). Mouse embryos homozygou ... Full text Link to item Cite

The mammalian Tolloid-like 1 gene, Tll1, is necessary for normal septation and positioning of the heart.

Journal Article Development · June 1999 Mammalian Tolloid-like 1 (mTLL-1) is an astacin-like metalloprotease, highly similar in domain structure to the morphogenetically important proteases bone morphogenetic protein-1 (BMP-1) and Drosophila Tolloid. To investigate possible roles for mTLL-1 in m ... Full text Link to item Cite

Bmp4 is required for the generation of primordial germ cells in the mouse embryo.

Journal Article Genes Dev · February 15, 1999 Featured Publication In many organisms the allocation of primordial germ cells (PGCs) is determined by the inheritance of maternal factors deposited in the egg. However, in mammals, inductive cell interactions are required around gastrulation to establish the germ line. Here, ... Full text Link to item Cite

Morphogenesis.

Journal Article Cell · January 22, 1999 Featured Publication Full text Link to item Cite

Role of the angiotensin type 2 receptor gene in congenital anomalies of the kidney and urinary tract, CAKUT, of mice and men.

Journal Article Mol Cell · January 1999 Angiotensin type 2 receptor gene null mutant mice display congenital anomalies of the kidney and urinary tract (CAKUT). Various features of mouse CAKUT impressively mimic human CAKUT. Studies of the human type 2 receptor (AGTR2) gene in two independent coh ... Full text Link to item Cite

BMP4 is essential for lens induction in the mouse embryo.

Journal Article Genes Dev · December 1, 1998 Featured Publication Vertebrate lens development is a classical model system for studying embryonic tissue interactions. Little is known, however, about the molecules mediating such inductive events. Here, we show that Bmp4, which is expressed strongly in the optic vesicle and ... Full text Link to item Cite

A mouse homologue of FAST-1 transduces TGF beta superfamily signals and is expressed during early embryogenesis.

Journal Article Mech Dev · December 1998 The transcription factor FAST-1 has recently been shown to play a key role in the specification of mesoderm by TGF beta superfamily signals in the early Xenopus embryo. We have cloned Fast1, a mouse homologue of Xenopus FAST-1, and characterized its expres ... Full text Link to item Cite

Formation of Rathke's pouch requires dual induction from the diencephalon.

Journal Article Development · December 1998 Targeted disruption of the homeobox gene T/ebp (Nkx2.1, Ttf1, Titf1) in mice results in ablation of the pituitary. Paradoxically, while T/ebp is expressed in the ventral diencephalon during forebrain formation, it is not expressed in Rathke's pouch or in t ... Full text Link to item Cite

Mutations of the forkhead/winged-helix gene, FKHL7, in patients with Axenfeld-Rieger anomaly.

Journal Article Am J Hum Genet · November 1998 Genetic linkage, genome mismatch scanning, and analysis of patients with alterations of chromosome 6 have indicated that a major locus for development of the anterior segment of the eye, IRID1, is located at 6p25. Abnormalities of this locus lead to glauco ... Full text Link to item Cite

Angiotensin induces the urinary peristaltic machinery during the perinatal period.

Journal Article J Clin Invest · October 15, 1998 The embryonic development of mammalian kidneys is completed during the perinatal period with a dramatic increase in urine production, as the burden of eliminating nitrogenous metabolic waste shifts from the placenta to the kidney. This urine is normally re ... Full text Link to item Cite

Epithelial/mesenchymal interactions and branching morphogenesis of the lung.

Journal Article Curr Opin Genet Dev · August 1998 The establishment of branched tubular epithelial structures is critical for the viability of multicellular organisms: the tracheal system in Drosophila and the vertebrate lung being two such structures. Although there are obvious differences in the complex ... Full text Link to item Cite

The forkhead/winged helix gene Mf1 is disrupted in the pleiotropic mouse mutation congenital hydrocephalus.

Journal Article Cell · June 12, 1998 Mf1 encodes a forkhead/winged helix transcription factor expressed in many embryonic tissues, including prechondrogenic mesenchyme, periocular mesenchyme, meninges, endothelial cells, and kidney. Homozygous null Mf1lacZ mice die at birth with hydrocephalus ... Full text Link to item Cite

Comparison of the expression of three highly related genes, Fgf8, Fgf17 and Fgf18, in the mouse embryo.

Journal Article Mech Dev · June 1998 In mammals, 16 members of the Fgf family have so far been described with diverse roles in embryonic cell growth and differentiation. Here, we report the expression from early streak stage to midgestation of two newly-identified murine genes, Fgf17 and Fgf1 ... Full text Link to item Cite

Altered wound healing in mice lacking a functional osteopontin gene (spp1).

Journal Article J Clin Invest · April 1, 1998 Osteopontin (OPN) is an arginine-glycine-aspartate (RGD)- containing glycoprotein encoded by the gene secreted phosphoprotein 1 (spp1). spp1 is expressed during embryogenesis, wound healing, and tumorigenesis; however, its in vivo functions are not well un ... Full text Link to item Cite

Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens.

Journal Article EMBO J · April 1, 1998 SPARC (secreted protein acidic and rich in cysteine, also known as osteonectin/BM40) is a secreted Ca2+-binding glycoprotein that interacts with a range of extracellular matrix molecules, including collagen IV. It is widely expressed during embryogenesis, ... Full text Link to item Cite

Bone morphogenetic protein 8A plays a role in the maintenance of spermatogenesis and the integrity of the epididymis.

Journal Article Development · March 1998 The murine Bmp8a and Bmp8b genes are tightly linked on mouse chromosome 4 and have similar expression during reproduction. Previous studies have shown that targeted mutagenesis of Bmp8b causes male infertility due to germ cell degeneration. To investigate ... Full text Link to item Cite

Angiotensinogen gene null-mutant mice lack homeostatic regulation of glomerular filtration and tubular reabsorption.

Journal Article Kidney Int · March 1998 Chronic volume depletion by dietary salt restriction causes marked decrease in glomerular filtration rate (GFR) with little increase in urine osmolality in angiotensinogen gene null mutant (Agt-/-) mice. Moreover, urine osmolality is insensitive to both wa ... Full text Link to item Cite

Mouse Mesenchyme forkhead 2 (Mf2): expression, DNA binding and induction by sonic hedgehog during somitogenesis.

Journal Article Mech Dev · January 1998 Cloning and sequencing of mouse Mf2 (mesoderm/mesenchyme forkhead 2) cDNAs revealed an open reading frame encoding a putative protein of 492 amino acids which, after in vitro translation, binds to a DNA consensus sequence. Mf2 is expressed at high levels i ... Full text Link to item Cite

Mice lacking Bmp6 function.

Journal Article Dev Genet · 1998 Bmp6, a member of the 60A subgroup of bone morphogenetic proteins (BMPs), is expressed in diverse sites in the developing mouse embryo from preimplantation stages onwards. To evaluate roles for Bmp6 signaling in vivo, gene targeting was used to generate a ... Full text Link to item Cite

Skeletal abnormalities in doubly heterozygous Bmp4 and Bmp7 mice.

Journal Article Dev Genet · 1998 Analysis of the skeletal phenotypes caused by the genetic inactivation of individual Bmps, along with the study of their expression patterns, suggest possible functional redundancy of these molecules. To investigate the effect on skeleton development of th ... Full text Link to item Cite

Cloning and characterization of developmental endothelial locus-1: an embryonic endothelial cell protein that binds the alphavbeta3 integrin receptor.

Journal Article Genes Dev · January 1, 1998 We have taken advantage of an enhancer trap event in a line of transgenic mice to identify a unique developmentally regulated endothelial cell locus (Del1). The protein encoded in this locus contains three EGF-like repeats homologous to those in Notch and ... Full text Link to item Cite

Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung.

Journal Article Development · December 1997 During mouse lung morphogenesis, the distal mesenchyme regulates the growth and branching of adjacent endoderm. We report here that fibroblast growth factor 10 (Fgf10) is expressed dynamically in the mesenchyme adjacent to the distal buds from the earliest ... Full text Link to item Cite

Evidence for the involvement of the Gli gene family in embryonic mouse lung development.

Journal Article Dev Biol · August 15, 1997 Murine Gli, Gli2, and Gli3 are zinc finger genes related to Drosophila cubitus interuptus, a component of the hedgehog signal transduction pathway. In the embryonic lung, all three Gli genes are strongly expressed at the pseudoglandular stage, in distinct ... Full text Link to item Cite

Haploinsufficient phenotypes in Bmp4 heterozygous null mice and modification by mutations in Gli3 and Alx4.

Journal Article Dev Biol · August 15, 1997 Bone morphogenetic protein 4 (Bmp4), a vertebrate homolog of Drosophila decapentaplegic (dpp), encodes a signaling protein with multiple functions during embryogenesis. Most mouse embryos homozygous for the Bmp4(tm1blh) null allele die around the time of g ... Full text Link to item Cite

Bone morphogenetic proteins (BMPs) as regulators of dorsal forebrain development.

Journal Article Development · June 1997 Bone Morphogenetic Proteins (BMPs) play crucial roles in a variety of developmental processes, but their functions during early vertebrate brain development are largely unknown. To investigate this problem, we have compared by in situ hybridization the exp ... Full text Link to item Cite

Disruption of PAX6 function in mice homozygous for the Pax6Sey-1Neu mutation produces abnormalities in the early development and regionalization of the diencephalon.

Journal Article Mech Dev · June 1997 Pax6 expression in the diencephalon of the mouse embryo is restricted both antero-posteriorly and dorso-ventrally, with changes in level occurring at prosomere boundaries. Small eye (Pax6Sey-1Neu) mice homozygous for Pax6 mutations have multiple defects in ... Full text Link to item Cite

The winged helix gene, Mf3, is required for normal development of the diencephalon and midbrain, postnatal growth and the milk-ejection reflex.

Journal Article Development · April 1997 The mouse Mf3 gene, also known as Fkh5 and HFH-e5.1, encodes a winged helix/forkhead transcription factor. In the early embryo, transcripts for Mf3 are restricted to the presomitic mesoderm and anterior neurectoderm and mesoderm. By 9.5 days post coitum, e ... Full text Link to item Cite

The winged helix transcription factor MFH1 is required for proliferation and patterning of paraxial mesoderm in the mouse embryo.

Journal Article Genes Dev · April 1, 1997 The gene mfh1, encoding a winged helix/forkhead domain transcription factor, is expressed in a dynamic pattern in paraxial and presomitic mesoderm and developing somites during mouse embryogenesis. Expression later becomes restricted to condensing mesenchy ... Full text Link to item Cite

The bZIP transcription factor LCR-F1 is essential for mesoderm formation in mouse development.

Journal Article Genes Dev · March 15, 1997 LCR-F1 is a mammalian bZIP transcription factor containing a basic amino acid domain highly homologous to a domain in the Drosophila Cap 'N' Collar and Caenorhabditis elegans SKN-1 proteins. LCR-F1 binds to AP1-like sequences in the human beta-globin locus ... Full text Link to item Cite

Intestinal tumorigenesis is suppressed in mice lacking the metalloproteinase matrilysin.

Journal Article Proc Natl Acad Sci U S A · February 18, 1997 Matrix metalloproteinases (MMPs) classically have been implicated in basement membrane destruction associated with late-stage tumor cell invasion and metastasis. However, recent studies have demonstrated that one MMP family member, matrilysin, is expressed ... Full text Link to item Cite

Involvement of Sonic hedgehog (Shh) in mouse embryonic lung growth and morphogenesis.

Journal Article Development · January 1997 Branching morphogenesis of the embryonic lung requires interactions between the epithelium and the mesenchyme. Previously, we reported that Sonic hedgehog (Shh) transcripts are present in the epithelium of the developing mouse lung, with highest levels in ... Full text Link to item Cite

Evidence that Mothers-against-dpp-related 1 (Madr1) plays a role in the initiation and maintenance of spermatogenesis in the mouse.

Journal Article Mech Dev · January 1997 We have cloned a mouse cDNA encoding a Mothers-against-dpp (MAD)-related protein, MADR1. Madr1 is ubiquitously expressed in the mouse embryo, indicating a broad function in a variety of tissue during embryogenesis, potentially relaying signals of numerous ... Full text Link to item Cite

Branching morphogenesis of the lung: new models for a classical problem.

Journal Article Cold Spring Harb Symp Quant Biol · 1997 Link to item Cite

Failure of ventral body wall closure in mouse embryos lacking a procollagen C-proteinase encoded by Bmp1, a mammalian gene related to Drosophila tolloid.

Journal Article Development · November 1996 The mouse bone morphogenetic protein1 (Bmp1) gene encodes a secreted astacin metalloprotease that cleaves the COOH-propeptide of procollagen I, II and III. BMP-1 is also related to the product of the Drosophila patterning gene, tolloid (tld), which enhance ... Full text Link to item Cite

Bone morphogenetic proteins in development.

Journal Article Curr Opin Genet Dev · August 1996 The bone morphogenetic proteins (BMPs) constitute a large family of cytokines related to members of the transforming growth factor-beta superfamily. Recent evidence, in particular from gene targeting experiments in the mouse, indicates that BMPs are requir ... Full text Link to item Cite

Evidence that mouse Bmp8a (Op2) and Bmp8b are duplicated genes that play a role in spermatogenesis and placental development.

Journal Article Mech Dev · July 1996 We have identified two highly conserved mouse genes encoding bone morphogenetic protein 8A (BMP8A/OP2) and 8B (BMP8B). The two loci are tightly linked on chromosome 4, suggesting that they arose through a recent gene duplication. Contrary to previous repor ... Full text Link to item Cite

The gene encoding bone morphogenetic protein 8B is required for the initiation and maintenance of spermatogenesis in the mouse.

Journal Article Genes Dev · July 1, 1996 Bone morphogenetic protein 8B (BMP8B) is a member of the TGFbeta superfamily of growth factors. In the mouse, Bmp8b is expressed in male germ cells of the testis and trophoblast cells of the placenta, suggesting that it has a role in spermatogenesis and re ... Full text Link to item Cite

The chromosomal mapping of four genes encoding winged helix proteins expressed early in mouse development.

Journal Article Genomics · June 1, 1996 Members of the winged helix family of transcription factors are required for the normal embryonic development of the mouse. Using the interspecific backcross panel from The Jackson Laboratory, we have determined the chromosomal locations of four genes that ... Full text Link to item Cite

Evidence from normal expression and targeted misexpression that bone morphogenetic protein (Bmp-4) plays a role in mouse embryonic lung morphogenesis.

Journal Article Development · June 1996 Epithelial-mesenchymal interactions are critical for the branching and differentiation of the lung, but the mechanisms involved are still unclear. To investigate this problem in mouse embryonic lung, we have studied the temporal and spatial expression of g ... Full text Link to item Cite

Bone morphogenetic protein-4 (BMP-4) acts during gastrula stages to cause ventralization of Xenopus embryos.

Journal Article Development · May 1996 Injection of RNA encoding BMP-4 into the early Xenopus embryo suppresses formation of dorsal and anterior cell types. To understand this phenomenon, it is necessary to know the stage at which BMP-4 acts. In this paper, we present three lines of evidence sh ... Full text Link to item Cite

PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum.

Journal Article Development · March 1996 It has been proposed that the Xenopus homeobox gene, XlHbox8, is involved in endodermal differentiation during pancreatic and duodenal development (Wright, C.V.E., Schnegelsberg, P. and De Robertis, E.M. (1988). Development 105, 787-794). To test this hypo ... Full text Link to item Cite

Enhancer analysis of the mouse HNF-3 beta gene: regulatory elements for node/notochord and floor plate are independent and consist of multiple sub-elements.

Journal Article Genes Cells · January 1996 BACKGROUND: Axial pattern formation in vertebrate embryos depends on signals from the node and, later, from the notochord and floor plate. Previous studies have shown that HNF-3 beta, a member of the winged-helix transcription factor family, plays key role ... Full text Link to item Cite

Gene targeting in mice reveals a requirement for angiotensin in the development and maintenance of kidney morphology and growth factor regulation.

Journal Article J Clin Invest · December 1995 Elevated levels of endogenous angiotensin can cause hypertensive nephrosclerosis as a result of the potent vasopressor action of the peptide. We have produced by gene targeting mice homozygous for a null mutation in the angiotensinogen gene (Atg-1-). Postn ... Full text Link to item Cite

Nodal-related signals induce axial mesoderm and dorsalize mesoderm during gastrulation.

Journal Article Development · November 1995 Mouse embryos homozygous for a null mutation in nodal arrest development at early gastrulation and contain little or no embryonic mesoderm. Here, two Xenopus nodal-related genes (Xnr-1 and Xnr-2) are identified and shown to be expressed transiently during ... Full text Link to item Cite

Expression of bone morphogenetic protein-4 (BMP-4), bone morphogenetic protein-7 (BMP-7), fibroblast growth factor-8 (FGF-8) and sonic hedgehog (SHH) during branchial arch development in the chick.

Journal Article Mech Dev · November 1995 Expression of Fgf-8, Bmp-4, Bmp-7, and shh in the branchial arches of the chick embryo is examined by in situ hybridization. Fgf-8 expression is initially broad and diffuse, becoming more tightly restricted, particularly in the epithelium of the posterior ... Full text Link to item Cite

Effects on blood pressure and exploratory behaviour of mice lacking angiotensin II type-2 receptor.

Journal Article Nature · October 26, 1995 There are two major angiotensin II receptor isoforms, AT1 and AT2. AT1 mediates the well-known pressor and mitogenic effects of angiotensin II, but the signalling mechanism and physiological role of AT2 has not been established. Its abundant expression in ... Full text Link to item Cite

Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse.

Journal Article Genes Dev · September 1, 1995 Bone morphogenetic protein-4 (BMP-4) is a member of the TGF-beta superfamily of polypeptide signaling molecules, closely related to BMP-2 and to Drosophila decapentaplegic (DPP). To elucidate the role of BMP-4 in mouse development the gene has been inactiv ... Full text Link to item Cite

Molecular and phenotypic characterization of a new mouse insertional mutation that causes a defect in the distal vertebrae of the spine.

Journal Article Genetics · July 1995 We have identified and characterized the phenotype of a new insertional mutation in one line of transgenic mice. Mice carrying this mutation, which we have designated TgN(Imusd)370Rpw, display undulations of the vertebrae giving rise to a novel kinky-tail ... Full text Link to item Cite

Colocalization of BMP 7 and BMP 2 RNAs suggests that these factors cooperatively mediate tissue interactions during murine development.

Journal Article Mech Dev · March 1995 Members of the bone morphogenetic protein (BMP) class of transforming growth factor beta (TGF beta)-related molecules have been implicated in a variety of inductive processes throughout vertebrate development. The 60A subclass of BMPs contains at least fou ... Full text Link to item Cite

Of bioethics and embryos

Journal Article Scientist · February 6, 1995 Cite

Chemical skin carcinogenesis is prevented in mice by the induced expression of a TGF-beta related transgene.

Journal Article Teratog Carcinog Mutagen · 1995 Skin papillomas and squamous cell carcinomas (SCCs) are induced in mice by tumor initiation with a carcinogen followed by tumor promotion with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). These usually arise from preneoplastic lesions char ... Full text Link to item Cite

Embryo research revisited.

Journal Article Hastings Cent Rep · 1995 Link to item Cite

Developmental expression of renal angiotensin II receptor genes in the mouse.

Journal Article Kidney Int · January 1995 The cellular distribution of angiotensin II type 1 (AT1) and type 2 (AT2) receptor mRNA was examined in mouse kidneys at several embryonic stages (12 to 18 days; 19 days = full term) and up to three weeks after birth by in situ hybridization. The expressio ... Full text Link to item Cite

The TGF-β-related signalling system in mouse development

Journal Article Seminars in Developmental Biology · January 1, 1995 Secreted polypeptide signalling molecules of the TGF- superfamily play critical roles during mouse development. There are 20 known members of this diverse family in the mouse, including the TGF-s, inhibin/activins, BMPs, GDFs, and nodal. Mutations in genes ... Full text Cite

Upside-down ideas vindicated

Journal Article Nature · 1995 Cite

Developmental signalling. Sorting out the signals.

Journal Article Curr Biol · December 1, 1994 New insights into the developmental roles played by the TGF-beta family of signalling molecules come from the identification in Drosophila of two transmembrane receptors encoded by the thick veins and saxophone genes. ... Full text Link to item Cite

Mouse embryonic germ (EG) cell lines: transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines.

Journal Article Development · November 1994 Primordial germ cells of the mouse cultured on feeder layers with leukemia inhibitory factor, Steel factor and basic fibroblast growth factor give rise to cells that resemble undifferentiated blastocyst-derived embryonic stem cells. These primordial germ c ... Full text Link to item Cite

TGF-beta related genes in development.

Journal Article Curr Opin Genet Dev · August 1994 Embryonic induction is the process by which signals from one cell population change the developmental fate of another. Polypeptides related to growth factors are one group of molecules mediating many inductive events. Recent data on the embryonic expressio ... Full text Link to item Cite

Embryonic expression of mouse bone morphogenetic protein-1 (BMP-1), which is related to the Drosophila dorsoventral gene tolloid and encodes a putative astacin metalloendopeptidase.

Journal Article Dev Biol · May 1994 cDNAs encoding a mouse gene closely related to human bone morphogenetic protein-1 (BMP-1) have been isolated from an 8.5-day p.c. embryo cDNA library. Analysis of the predicted amino acid sequence shows that the protein contains a putative zinc-binding ast ... Full text Link to item Cite

Acceleration of mammary neoplasia in transforming growth factor alpha transgenic mice by 7,12-dimethylbenzanthracene.

Journal Article Cancer Res · April 1, 1994 A mouse mammary tumor virus enhancer/promoter-transforming growth factor alpha transgenic mouse model has been described in which mammary tumors develop (Y. Matsui et al., Cell, 61: 1147-1155, 1990). In Line 29, spontaneous mammary tumors do not develop be ... Link to item Cite

On-line coupling of in vivo microdialysis sampling with capillary electrophoresis.

Journal Article Anal Chem · March 1, 1994 Microdialysis sampling has become an important means of continuously monitoring reactions in vivo. This sampling technique places a constraint on the analysis method because of the very small sample volume provided. On the other hand, microdialysis provide ... Full text Link to item Cite

HNF-3 beta as a regulator of floor plate development.

Journal Article Cell · January 14, 1994 The transcription factor gene HNF-3 beta is expressed in the ventral midline of the mouse embryonic neural tube, including the floor plate, a structure important for dorsoventral patterning and axonal guidance. To assess HNF-3 beta function, the gene has b ... Full text Link to item Cite

Growth factors in development: the role of TGF-beta related polypeptide signalling molecules in embryogenesis.

Journal Article Dev Suppl · 1994 Embryonic induction, the process by which signals from one cell population influence the fate of another, plays an essential role in the development of all organisms so far studied. In many cases, the signalling molecules belong to large families of highly ... Link to item Cite

Embryonic expression of Lim-1, the mouse homolog of Xenopus Xlim-1, suggests a role in lateral mesoderm differentiation and neurogenesis.

Journal Article Dev Biol · January 1994 cDNAs encoded by the mouse homolog (Lim-1) of the Xenopus LIM-class homeobox gene Xlim-1 have been isolated from an 8.5-day mouse embryo cDNA library. Nucleotide and deduced amino acid sequences show a high degree of identity with Xlim-1 in the LIM and hom ... Full text Link to item Cite

Embryonic germ cell lines and their derivation from mouse primordial germ cells.

Journal Article Ciba Found Symp · 1994 When primordial germ cells of the mouse are cultured on feeder layers with the addition of the polypeptide signalling molecules leukaemia inhibitory factor, Steel factor and basic fibroblast growth factor they give rise to cells that resemble undifferentia ... Full text Link to item Cite

Growth factors in development: The role of TGF-β related polypeptide signalling molecules in embryogenesis

Journal Article Development · January 1, 1994 Embryonic induction, the process by which signals from one cell population influence the fate of another, plays an essential role in the development of all organisms so far studied. In many cases, the signalling molecules belong to large families of highly ... Cite

Inhibition of mammary duct development but not alveolar outgrowth during pregnancy in transgenic mice expressing active TGF-beta 1.

Journal Article Genes Dev · December 1993 The transforming growth factors beta (TGFs-beta) are potent inhibitors of cell proliferation and are usually secreted in a latent form. TGF-beta 1, TGF-beta 2, and TGF-beta 3 are expressed in distinct but overlapping patterns in the developing mouse mammar ... Full text Link to item Cite

Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo.

Journal Article Development · May 1993 Four genes encoding fork-head-domain-containing proteins (FD genes) have been isolated from a mouse 8.5 days post coitum (p.c.) embryo cDNA library. Two are mouse homologues of rat HNF-3 beta and HNF-3 alpha. The other two are novel and have been named MF- ... Full text Link to item Cite

Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation.

Journal Article Nature · February 11, 1993 During gastrulation, the three germ layers of the embryo are formed and organized along the anterior-posterior body axis. In the mouse, gastrulation involves the delamination of ectodermal cells through the primitive streak and their differentiation into m ... Full text Link to item Cite

Transgenic mice as a model to study the role of TGF-beta-related molecules in hair follicles.

Journal Article Genes Dev · February 1993 There is increasing evidence that members of the TGF-beta superfamily are important regulators of epithelial growth and differentiation in vivo. Here, transgenic mice have been used to study the role of the TGF-beta-related growth factors BMP-2 and BMP-4 i ... Full text Link to item Cite

Biosynthesis and in vivo localization of the decapentaplegic-Vg-related protein, DVR-6 (bone morphogenetic protein-6).

Journal Article J Cell Biol · January 1993 DVR-6 (BMP-6 or Vgr-1) is a member of the TGF-beta superfamily of polypeptide signaling molecules. In situ hybridization studies have previously shown that DVR-6 RNA is expressed in a variety of cell types in the mouse embryo, but no information has been a ... Full text Link to item Cite

Inhibiting inhibin

Journal Article Current Biology · January 1, 1993 Full text Cite

Women in science.

Journal Article Nature · November 19, 1992 Full text Link to item Cite

Determination of intracellular species at the level of a single erythrocyte via capillary electrophoresis with direct and indirect fluorescence detection.

Journal Article Anal Chem · November 15, 1992 Intracellular contents reflect the specific history of a cell including innate physiological heterogeneity as well as differing levels of exposure to environmental influences. A method capable of analyzing a variety of species from within a single human er ... Full text Link to item Cite

Isolation of Vgr-2, a novel member of the transforming growth factor-beta-related gene family.

Journal Article Mol Endocrinol · November 1992 A cDNA clone, Vgr-2, with homology to certain members of the transforming growth factor-beta superfamily has been isolated from a mouse embryo cDNA library. The encoded protein shows significant similarity to members of the Vg-1/decapentaplegic/bone morpho ... Full text Link to item Cite

Evidence that Hensen's node is a site of retinoic acid synthesis.

Journal Article Nature · September 17, 1992 Hensen's node of amniotes, like the Spemann organizer of amphibians, can induce a second body axis when grafted into a host embryo. The avian node, as well as several midline structures originating from it (notochord, floor plate), can also induce digit pa ... Full text Link to item Cite

Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture.

Journal Article Cell · September 4, 1992 Steel factor (SF) and LIF (leukemia inhibitory factor) synergistically promote the proliferation and survival of mouse primordial germ cells (PGCs), but only for a limited time period in culture. We show here that addition of bFGF to cultures in the presen ... Full text Link to item Cite

DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction.

Journal Article Development · June 1992 Establishment of mesodermal tissues in the amphibian body involves a series of inductive interactions probably elicited by a variety of peptide growth factors. Results reported here suggest that mesodermal patterning involves an array of signalling molecul ... Full text Link to item Cite

Distinctive patterns of hyperplasia in transgenic mice with mouse mammary tumor virus transforming growth factor-alpha. Characterization of mammary gland and skin proliferations.

Journal Article Am J Pathol · May 1992 Eight lines of transgenic mice expressing a mouse mammary tumor virus (MMTV) human transforming growth factor-alpha (TGF alpha) fusion gene were established. Three lines with distinctive phenotypes are presented. All have proliferative changes of the mamma ... Link to item Cite

Expression and modification of Hox 2.1 protein in mouse embryos.

Journal Article Mech Dev · May 1992 A polyclonal antibody, alpha Hox 2.1a, has been generated and used to immunolocalize Hox 2.1 protein in mouse embryos. Protein is present in nuclei of all tissues previously shown to express Hox 2.1 RNA. In addition, protein is seen in somites and proximal ... Full text Link to item Cite

Expression of TIMP in fetal and adult mouse tissues studied by in situ hybridization.

Journal Article Matrix Suppl · 1992 We have studied the expression of TIMP mRNA during mouse embryogenesis and in adult tissues using ribonuclease protection assays and in situ hybridization. Low levels of transcripts were found in many tissues, including embryonic kidney, amnion, lung and m ... Link to item Cite

The TGF-beta-related DVR gene family in mammalian development.

Journal Article Ciba Found Symp · 1992 The genes that encode the bone morphogenetic proteins and the Vg-related proteins are mammalian members of a group of TGF-beta-related genes, designated the DVR family, that includes the decapentaplegic gene of Drosophila and the Vg1 gene of Xenopus. Membe ... Full text Link to item Cite

Editorial overview

Journal Article Current Opinion in Cell Biology · January 1, 1992 Full text Cite

Mouse small eye results from mutations in a paired-like homeobox-containing gene.

Journal Article Nature · December 19, 1991 Small eye (Sey) in mouse is a semidominant mutation which in the homozygous condition results in the complete lack of eyes and nasal primordia. On the basis of comparative mapping studies and on phenotypic similarities, Sey has been suggested to be homolog ... Full text Link to item Cite

The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells.

Journal Article Nucleic Acids Res · October 25, 1991 Wilms' tumor, a pediatric nephroblastoma, has been associated with genetic alterations of the 11p13 and 11p15 regions. The introduction of a der(11) chromosome into the G401 Wilms' tumor cell line has been shown previously to revert the tumorigenic phenoty ... Full text Link to item Cite

Effect of Steel factor and leukaemia inhibitory factor on murine primordial germ cells in culture.

Journal Article Nature · October 24, 1991 Despite the importance of germ cells to the survival of species, surprisingly little is known about their embryological origin, proliferation, migration and entry into mitotic arrest or meiosis. Mutations in the murine Dominant White Spotting (W) and Steel ... Full text Link to item Cite

Man's inhumanity.

Journal Article Nature · February 14, 1991 Full text Link to item Cite

Involvement of Bone Morphogenetic Protein-4 (BMP-4) and Vgr-1 in morphogenesis and neurogenesis in the mouse.

Journal Article Development · February 1991 Bone Morphogenetic Protein-4 (BMP-4) and Vgr-1 are members of the TGF-beta gene family most closely related to the Drosophila Decapentaplegic and Xenopus Vg-1 genes. Members of this gene family have been implicated in diverse processes during embryogenesis ... Full text Link to item Cite

The DVR gene family in embryonic development.

Journal Article Trends Genet · 1991 The DVR gene family consists of at least 15 members, including decapentaplegic from Drosophila, Xenopus Vg1 and the mammalian bone morphogenetic protein genes, encoding secreted proteins closely related to transforming growth factor beta Genetic and bioche ... Full text Link to item Cite

Editorial overview

Journal Article Current Opinion in Cell Biology · January 1, 1991 Full text Cite

Embryonic expression of a haematopoietic growth factor encoded by the Sl locus and the ligand for c-kit.

Journal Article Nature · October 18, 1990 Mice carrying mutations at the W (Dominant white spotting) and Sl (Steel) loci develop abnormalities in three independent systems: neural crest-derived melanocytes, primordial germ cells and haematopoietic stem cells. Consequently, homozygotes of viable mu ... Full text Link to item Cite

Differential expression of genes encoding TGFs beta 1, beta 2, and beta 3 during murine palate formation.

Journal Article Dev Biol · October 1990 Transforming growth factor beta 1 (TGF beta 1) has been shown to have multiple effects on primary cultures of palate-derived cell types. We report the analysis, by in situ hybridization, of RNA expression for three different TGF beta isoforms (TGF beta 1, ... Full text Link to item Cite

In situ hybridization analysis of TGF beta 3 RNA expression during mouse development: comparative studies with TGF beta 1 and beta 2.

Journal Article Development · October 1990 To date, three closely-related TGF beta genes have been found in the mouse; TGF beta 1, TGF beta 2 and TGF beta 3. Previous experiments have indicated that TGF beta 1 and TGF beta 2 may play important roles during mouse embryogenesis. The present study now ... Full text Link to item Cite

Organogenesis and pattern formation in the mouse: RNA distribution patterns suggest a role for bone morphogenetic protein-2A (BMP-2A).

Journal Article Development · August 1990 Bone morphogenetic protein-2A (BMP-2A) is a member of the transforming growth factor beta (TGF beta) gene family that has been implicated in cartilage and bone formation. Here we use in situ hybridization to show that BMP-2A RNA is expressed in a variety o ... Full text Link to item Cite

Development of mammary hyperplasia and neoplasia in MMTV-TGF alpha transgenic mice.

Journal Article Cell · June 15, 1990 To study the role of transforming growth factor alpha (TGF alpha) in normal mammary development and mammary neoplasia in vivo, we have generated transgenic mice in which a human TGF alpha cDNA is expressed under the control of the MMTV enhancer/promoter. O ... Full text Link to item Cite

Location of the gene involving the small eye mutation on mouse chromosome 2 suggests homology with human aniridia 2 (AN2).

Journal Article Genomics · June 1990 Using an interspecific backcross, we have mapped the gene involved in the mouse Small eye mutation (SeyMH) relative to six cloned markers on chromosome 2 (Hox-5.1, Cas-1, Fshb, Bmp-2a, and ld) and the agouti locus. The results suggest that the Sey gene map ... Full text Link to item Cite

Molecular and genetic characterization of a radiation-induced structural rearrangement in mouse chromosome 2 causing mutations at the limb deformity and agouti loci.

Journal Article Proc Natl Acad Sci U S A · April 1990 Molecular characterization of mutations in the mouse, particularly those involving agent-induced major structural alterations, is proving to be useful for correlating the structure and expression of individual genes with their function in the whole organis ... Full text Link to item Cite

The HOX-5 and surfeit gene clusters are linked in the proximal portion of mouse chromosome 2.

Journal Article Genomics · April 1990 Using an interspecies backcross, we have mapped the HOX-5 and surfeit (surf) gene clusters within the proximal portion of mouse chromosome 2. While the HOX-5 cluster of homeobox-containing genes has been localized to chromosome 2, bands C3-E1, by in situ h ... Full text Link to item Cite

Chromosomal localization of seven members of the murine TGF-beta superfamily suggests close linkage to several morphogenetic mutant loci.

Journal Article Genomics · March 1990 Chromosomal locations have been assigned to seven members of the TGF-beta superfamily using an interspecific mouse backcross. Probes for the Tgfb-1, -2, and -3, Bmp-2a and -3, and Vgr-1 genes recognized only single loci, whereas the Bmp-2b probe recognized ... Full text Link to item Cite

Growth factor-regulated proteases and extracellular matrix remodeling during mammalian development.

Journal Article Curr Top Dev Biol · 1990 Although specific details may vary from system to system, some general concepts have emerged from studies of the regulation of ECM components, proteases, and protease inhibitors by growth factors. Growth factors may be divided into those that enhance matri ... Full text Link to item Cite

Indirect fluorometric detection of tryptic digests separated by capillary zone electrophoresis.

Journal Article J Chromatogr Sci · January 1990 This work demonstrates the analytical utility of indirect fluorescence detection with capillary zone electrophoresis (CZE) for the analysis of trace quantities of macromolecular mixtures. Detection is based upon charge displacement and is not based upon an ... Full text Link to item Cite

Patterns of expression of murine Vgr-1 and BMP-2a RNA suggest that transforming growth factor-beta-like genes coordinately regulate aspects of embryonic development.

Journal Article Genes Dev · November 1989 The murine Vgr-1 (Vg-related) and BMP-2a (bone morphogenetic protein 2a) genes are members of the decapentaplegic subgroup of the transforming growth factor-beta (TGF beta) superfamily. Although genetic and biochemical studies suggest that the members of t ... Full text Link to item Cite

Distribution of expression of 2AR (osteopontin) in the embryonic mouse inner ear revealed by in situ hybridisation.

Journal Article Hear Res · September 1989 Using in situ hybridisation we have determined the distribution of expression of 2ar (also known as osteopontin, bone sialoprotein 1 or 44-kDa bone phosphoprotein) in the developing mouse inner ear. We have identified several discrete sites, both osteogeni ... Full text Link to item Cite

Localization of the mouse gene for secreted phosphoprotein 1 (Spp-1) (2ar, osteopontin, bone sialoprotein 1, 44-kDa bone phosphoprotein, tumor-secreted phosphoprotein) to chromosome 5, closely linked to Ric (Rickettsia resistance).

Journal Article Genomics · August 1989 We have used a cDNA probe for mouse secreted phosphoprotein 1 (Spp-1, also known as 2ar, osteopontin, bone sialoprotein 1, 44-kDa bone phosphotein, tumor-secreted protein) to find a restriction fragment length polymorphism in the gene from C57BL/6J and DBA ... Full text Link to item Cite

Expression of transforming growth factor beta 2 RNA during murine embryogenesis.

Journal Article Development · August 1989 We have studied the temporal and spatial expression of transforming growth factor beta 2 (TGF beta 2) RNA in mouse embryos from 10.5 days post coitum (p.c.) to 3 days post partum (p.p.) by in situ hybridization analysis. TGF beta 2 RNA is expressed in a va ... Full text Link to item Cite

Evidence for positive and negative regulatory elements in the 5'-flanking sequence of the mouse sparc (osteonectin) gene.

Journal Article J Biol Chem · July 25, 1989 We have investigated the role of 5'-flanking DNA sequences in regulating the expression of the murine Sparc (osteonectin) gene in parietal endoderm cells and in F9 embryonal carcinoma cells induced to differentiate into parietal endoderm with retinoic acid ... Link to item Cite

Vgr-1, a mammalian gene related to Xenopus Vg-1, is a member of the transforming growth factor beta gene superfamily.

Journal Article Proc Natl Acad Sci U S A · June 1989 The transforming growth factor beta (TGF-beta)-related products of the Xenopus Vg-1 and Drosophila decapentaplegic (DPP) genes have been implicated in the control of growth and differentiation during embryogenesis. We have isolated a mouse cDNA, Vgr-1, tha ... Full text Link to item Cite

Developmental expression of tissue inhibitor of metalloproteinase (TIMP) RNA.

Journal Article Development · March 1989 Single-stranded antisense RNA probes have been used to study the expression of the metalloproteinase inhibitor TIMP (tissue inhibitor of metalloproteinases), during mouse embryogenesis and in adult tissues. Using a sensitive RNase protection assay, low lev ... Full text Link to item Cite

Expression of genes for non-collagenous proteins during embryonic bone formation.

Journal Article Connect Tissue Res · 1989 In situ hybridization has been used to localize RNAs for a variety of non-collagenous proteins during embryogenesis of the mouse, with particular reference to bone formation. Transcripts of the Sparc (osteonectin) gene are first detected around 14.5d post ... Full text Link to item Cite

Gene targeting. Getting nearer the mark.

Journal Article Nature · November 24, 1988 Full text Link to item Cite

Expression of the homeobox gene, Hox 2.1, during mouse embryogenesis.

Journal Article Cell Differ Dev · November 1988 This article reviews recent studies on the expression of the homeobox gene, Hox 2.1, during mouse embryogenesis, using the technique of in situ hybridization. Differential hybridization of radiolabelled antisense versus sense strand RNA is first clearly de ... Full text Link to item Cite

Characterization of the mouse SPARC/osteonectin gene. Intron/exon organization and an unusual promoter region.

Journal Article J Biol Chem · August 15, 1988 Two overlapping cosmids have been isolated containing the entire murine gene for SPARC (osteonectin), a Ca2+-binding, phosphorylated glycoprotein associated with extracellular matrix synthesis and remodeling. The gene contains 10 exons and covers 26.5 kilo ... Link to item Cite

Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization.

Journal Article J Cell Biol · February 1988 2ar has been identified as a gene inducible by tumor promoters and growth factors in a variety of cultured mouse cell lines (Smith, J. H., and D. T. Denhardt. 1987. J. Cell. Biochem. 34:13-22). Sequence analysis shows that it codes for mouse osteopontin, a ... Full text Link to item Cite

Spatially restricted patterns of expression of the homeobox-containing gene Hox 2.1. during mouse embryogenesis.

Journal Article Development · January 1988 The mouse Hox 2.1 gene contains a homeobox sequence and is therefore a candidate for a vertebrate gene involved in the control of embryonic patterning or positional specification. To investigate this possibility, we have used in situ hybridization to deter ... Full text Link to item Cite

Small eye (Sey): a mouse model for the genetic analysis of craniofacial abnormalities.

Journal Article Development · 1988 Small eye (Sey) is a dominant mutation in the mouse affecting the embryonic development of the eyes and nose. In homozygous Sey/Sey embryos, the optic vesicles grow out but there is no lens induction and the nasal pits fail to develop. Scanning electron mi ... Full text Link to item Cite

Molecular analysis of the cDNA for human SPARC/osteonectin/BM-40: sequence, expression, and localization of the gene to chromosome 5q31-q33.

Journal Article Genomics · January 1988 Human cDNA clones encoding the extracellular calcium-binding, acidic glycoprotein known as SPARC, osteonectin, or BM-40 were isolated from a placental cDNA library. Two polyadenylated transcripts of 2.2 and 3.0 kb were detected in human tissues and culture ... Full text Link to item Cite

Temporal and tissue-specific expression of distinct retrovirus-like (VL30) elements during mouse development.

Journal Article Dev Biol · January 1988 We have analyzed the expression of VL30 retroviral RNA transcripts during mouse embryogenesis. VL30 RNA was found in all tissues examined from mid-gestation, but increased dramatically at later times in the extraembryonic amnion and visceral yolk sac, and ... Full text Link to item Cite

A morphological analysis endocrine tumour genesis in pancreas and anterior pituitary of AVP/SV40 transgenic mice.

Journal Article Virchows Arch A Pathol Anat Histopathol · 1988 Insertion into the mouse genome of the hybrid oncogene made up of bovine vasopressin gene derived 5' upstream sequences and the coding sequences of SV40 large T-antigen promoted tumours in anterior pituitary and endocrine pancreas of mice bearing this tran ... Full text Link to item Cite

Mice transgenic for a vasopressin-SV40 hybrid oncogene develop tumors of the endocrine pancreas and the anterior pituitary. A possible model for human multiple endocrine neoplasia type 1.

Journal Article Am J Pathol · December 1987 The authors have used transgenic mice to study the activity of a hybrid oncogene made up of 1.25 kb of 5' upstream sequences, derived from the bovine vasopressin gene, promoting the expression of the large T-antigen coding sequences of the early region of ... Link to item Cite

Calcium binding domains and calcium-induced conformational transition of SPARC/BM-40/osteonectin, an extracellular glycoprotein expressed in mineralized and nonmineralized tissues.

Journal Article Biochemistry · November 3, 1987 SPARC, BM-40, and osteonectin are identical or very closely related extracellular proteins of apparent Mr 43,000 (Mr 33,000 predicted from sequence). They were originally isolated from parietal endoderm cells, basement membrane producing tumors, and bone, ... Full text Link to item Cite

In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization.

Journal Article J Cell Biol · July 1987 In situ hybridization is used to survey the tissue-specific and developmental expression of the cloned mouse gene Sparc, coding for a protein homologous to the bovine Ca++-binding protein, osteonectin. High levels of SPARC RNA are found in osteoblasts and ... Full text Link to item Cite

Transcription of H-2 and Qa genes in embryonic and adult mice.

Journal Article EMBO J · May 1987 We have isolated cDNAs for three class I genes from an 8.5-day C57Bl/6 mouse embryo cDNA library. Two of these cDNAs encode the classical transplantation antigens, H-2Kb and H-2Db. The third is a novelly spliced form of a Qa region gene, Q9, which lacks se ... Full text Link to item Cite

Developmental and spatial patterns of expression of the mouse homeobox gene, Hox 2.1.

Journal Article Development · April 1987 The Hox 2.1 gene forms part of a cluster of homeobox-containing genes on mouse chromosome 11. Analysis of Hox 2.1 cDNAs isolated from an 8 1/2-day p.c. mouse embryo library predicts that the gene encodes a 269 amino acid protein (Mr, 29,432). This deduced ... Full text Link to item Cite

Close genetic and physical linkage between the murine haemopoietic growth factor genes GM-CSF and Multi-CSF (IL3).

Journal Article EMBO J · March 1987 The two murine haemopoietic growth factors, granulocyte-macrophage colony stimulating factor (GM-CSF) and Multi-CSF (interleukin 3) stimulate the proliferation and differentiation of an overlapping set of haemopoietic progenitor cells and are produced coor ... Full text Link to item Cite

Distinct patterns of glycosylation of colligin, a collagen-binding glycoprotein, and SPARC (osteonectin), a secreted Ca2+-binding glycoprotein. Evidence for the localisation of colligin in the endoplasmic reticulum.

Journal Article Eur J Biochem · February 16, 1987 Mouse parietal endoderm PYS cells were labelled with [2-3H]mannose for 16-24 h. Colligin, an Mr-47000 collagen-binding protein, and SPARC, a Mr-43000 protein, highly homologous to the Ca2+-binding protein osteonectin, were isolated from labelled cell extra ... Full text Link to item Cite

Molecular cloning of laminin.

Journal Article Methods Enzymol · 1987 Full text Link to item Cite

Phylogenetic distribution of Antennapedia-like homoeo boxes

Journal Article Nature · December 1, 1986 The homoeo box is a conserved protein-coding DNA sequence present in several genes in arthropods, annelids and vertebrates1-4. Two distinct classes of homoeo box have been identified-the Antennapedia (Antp) class1,5 and the engrailed class6-8. In insects, ... Full text Cite

Small eyes (Sey): a homozygous lethal mutation on chromosome 2 which affects the differentiation of both lens and nasal placodes in the mouse.

Journal Article J Embryol Exp Morphol · September 1986 Small eyes (Sey) is a semidominant, homozygous lethal mutation in the mouse (Roberts, 1967). It is allelic with SeyH, a radiation-induced homozygous prenatal lethal which has been mapped on chromosome 2. The effect of the Sey mutation is apparently limited ... Link to item Cite

Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11.

Journal Article EMBO J · August 1986 SPARC is a Mr 43,000 secreted, acidic, cysteine-rich glycoprotein homologous to 43K bovine endothelial 'culture shock' protein. We show here that it is encoded by a single gene localized to the central region of mouse chromosome 11. During development SPAR ... Full text Link to item Cite

Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000.

Journal Article EMBO J · July 1986 We describe the molecular cloning and characterization of a secreted, acidic, cysteine-rich glycoprotein (SPARC) of apparent Mr 43,000 which is a major product of mouse embryo parietal endoderm. These cells are specialized for the synthesis of a rapidly ex ... Full text Link to item Cite

The murine Hox-2 cluster of homeo box containing genes maps distal on chromosome 11 near the tail-short (Ts) locus.

Journal Article Cytogenet Cell Genet · 1986 Two probes derived from a mouse recombinant lambda-clone (H24.1), that contains a sequence closely homologous to the Drosophila antennapedia homeo box, were mapped to mouse chromosome (MMU) 11 by filter hybridization of somatic cell hybrid DNA. This sequen ... Full text Link to item Cite

A mouse homoeo box gene is expressed during embryogenesis and in adult kidney.

Journal Article Nature · October 24, 1985 The homoeo box sequence of Drosophila is an element located in several genes that regulate segmentation and segment identity; it has homologues in the genomes of vertebrate species and a number of homoeo box-containing recombinant DNA clones have been isol ... Full text Link to item Cite

Linkage of genes for laminin B1 and B2 subunits on chromosome 1 in mouse.

Journal Article In Vitro Cell Dev Biol · August 1985 We have used cDNA clones for the B1 and B2 subunits of laminin to find restriction fragment length DNA polymorphisms for the genes encoding these polypeptides in the mouse. Three alleles were found for LamB2 and two for LamB1 among the inbred mouse strains ... Full text Link to item Cite

Biosynthesis of EGF receptor, transferrin receptor and colligin by cultured human keratinocytes and the effect of retinoic acid.

Journal Article Exp Cell Res · July 1985 The biosynthesis of EGF and transferrin receptor by human keratinocytes in culture has been followed using specific monoclonal antibodies. In addition, keratinocytes are shown to synthesise a Mr 47 000 protein that binds to gelatin-Sepharose. Peptide mappi ... Full text Link to item Cite

Chromosomal assignments of the genes coding for human types II, III, and IV collagen: a dispersed gene family.

Journal Article Proc Natl Acad Sci U S A · May 1985 The human type II collagen gene, COL2A1, has been assigned to chromosome 12, the type III gene, COL3A1, to chromosome 2, and one of the type IV genes, COL4A1, to chromosome 13. These assignments were made by using cloned genes as probes on Southern blots o ... Full text Link to item Cite

Expression of c-fos in parietal endoderm, amnion and differentiating F9 teratocarcinoma cells.

Journal Article Differentiation · 1985 The expression of the cellular proto-oncogene, c-fos, in extra-embryonic tissues of the mouse was investigated using a v-fos DNA probe and an affinity-purified antiserum raised against a C-terminal synthetic peptide. At 13.5 days of development, parietal e ... Full text Link to item Cite

How is the mouse segmented?

Journal Article Trends in Genetics · January 1, 1985 During mouse embryogenesis the vertebrae, ribs, muscles and dermis are all derived from about 65 paired blocks of mesodermal cells - the somites - which are laid down sequentially along the body axis. This pattern of body segmentation is compared with that ... Full text Cite

Sequencing of laminin B chain cDNAs reveals C-terminal regions of coiled-coil alpha-helix.

Journal Article EMBO J · October 1984 cDNAs for laminin B chains have been isolated from a parietal endoderm cDNA library in pUC8 and pUC9. Identification is based on: ability to direct the synthesis in Escherichia coli of polypeptides carrying laminin antigen determinants, in vitro translatio ... Full text Link to item Cite

Pattern of serum protein gene expression in mouse visceral yolk sac and foetal liver.

Journal Article EMBO J · August 1984 We have shown by molecular hybridisation that the mRNAs for albumin, transferrin, apolipoprotein-A1, and alpha 1-antitrypsin are expressed at high levels in mouse visceral yolk sac. In contrast, the mRNAs for contrapsin (a plasma protease inhibitor) and th ... Full text Link to item Cite

Cell surface-associated proteins which bind native type IV collagen or gelatin.

Journal Article J Biol Chem · May 10, 1984 Gelatin coupled to Sepharose has been used to isolate [35S]methionine-labeled polypeptides of Mr = 47,000, 56,000, 62,000, and 65,000 from the 12,000 X g supernatant of detergent extracts of mouse embryo parietal endoderm cells. The polypeptides can also b ... Link to item Cite

Expression of EGF receptor and transferrin by F9 and PC13 teratocarcinoma cells.

Journal Article Differentiation · 1984 We document the time of appearance and the levels of two markers of differentiation during the formation of embryoid bodies by two embryonal carcinoma (EC) cell lines. Neither of these markers has been described before for EC cells differentiating in aggre ... Full text Link to item Cite

A scanning electron microscope study of the extraembryonic endoderm of the 8th-day mouse embryo.

Journal Article Differentiation · 1984 Late primitive streak embryos were dissected to reveal the junction between the visceral (VE) and parietal (PE) extraembryonic endoderm. Scanning electron microscopy showed that the two cell types differ markedly in their surface morphology and intercellul ... Full text Link to item Cite

Reichert's membrane as a model for studying the biosynthesis and assembly of basement membrane components.

Journal Article Ciba Found Symp · 1984 The major components of Reichert's membrane (laminin, type IV procollagen, entactin and heparan sulphate proteoglycan) are all synthesized by the parietal endoderm cells of the mouse embryo. Fibronectin is found mainly on the trophoblast side of Reichert's ... Full text Link to item Cite

Changes in the rate of laminin and entactin synthesis in F9 embryonal carcinoma cells treated with retinoic acid and cyclic amp.

Journal Article Dev Biol · October 1983 The synthesis of laminin A and B chains, and of entactin, has been measured in murine F9 embryonal carcinoma cells differentiating in response to retinoic acid and cyclic AMP. Undifferentiated cells synthesis low levels of laminin, amounting to approximate ... Full text Link to item Cite

Isolation of cDNA clones for basal lamina components: type IV procollagen.

Journal Article Nucleic Acids Res · September 24, 1983 We have isolated cDNA clones for mouse type IV procollagen from a library constructed from total poly A+RNA of 13.5 day mouse embryo parietal endoderm (PE) cells. In Northern analysis these clones hybridise to a 6.8 kb RNA which is abundant in embryonic PE ... Full text Link to item Cite

Co-expression of vimentin and cytokeratins in parietal endoderm cells of early mouse embryo.

Journal Article Nature · June 23, 1983 Of the five classes of intermediate filaments found in vertebrate tissues, the cytokeratins are considered unique to epithelial tissues, while vimentin is expressed by endothelial and mesenchymal cells. In neither case is the precise function of the filame ... Full text Link to item Cite

In vitro synthesis of laminin and entactin polypeptides.

Journal Article J Biol Chem · May 25, 1983 Total RNA and poly(A+) RNA, isolated from 13.5-day-old mouse embryo parietal endoderm cells and from differentiated F9 teratocarcinoma cells that synthesize laminin and entactin, were translated in the reticulocyte lysate. Antiserum raised against purified ... Link to item Cite

Carbohydrate changes in pre- and peri-implantation mouse embryos as detected by a monoclonal antibody.

Journal Article Cell Differ · January 1983 We have examined the tissue and embryonic distribution of an antigen on a large polysaccharide that is recognized by a monoclonal antibody, IIC3, prepared against F9 teratocarcinoma cells. By immunofluorescence the antigen is first detected on compacted mo ... Full text Link to item Cite

In vitro synthesis of type IV procollagen.

Journal Article J Biol Chem · December 25, 1982 Total RNA was isolated from parietal endoderm cells of 131/2-day mouse embryos that synthesize large amounts of type IV procollagen. In vitro translation of this RNA in the reticulocyte lysate supplemented with a ribonuclease inhibitor yielded two equally ... Link to item Cite

Synthesis and localization of two sulphated glycoproteins associated with basement membranes and the extracellular matrix.

Journal Article J Cell Biol · October 1982 Two sulphated glycoproteins (sgps) of apparent molecular weight (Mr) 180,000 and 150,000, are synthesized by murine PYS and PF HR9 parietal endoderm and Swiss 3T3 cells. The Mr 150,000 sgp has a similar chemical structure to the sulphated glycoprotein, C, ... Full text Link to item Cite

Localization of fibronectin, laminin-entactin, and entactin in Reichert's membrane by immunoelectron microscopy.

Journal Article EMBO J · 1982 Immunoelectron microscopy using protein A-colloidal gold complexes of different sizes was used to study the relative distribution of extracellular matrix glycoproteins within Reichert's membrane (RM) of 13.5-day mouse embryos. Labelling for fibronectin was ... Full text Link to item Cite

Studies on the biosynthesis of laminin by murine parietal endoderm cells.

Journal Article Eur J Biochem · September 1981 The biosynthesis and processing of the polypeptides A (Mr = 450 x 10(3)), B1 (Mr = 240 x 10(3)), B2 (Mr = 230 x 10(3)) and C (Mr = 150 x 10(3)) of the extracellular matrix protein, laminin, were studied in murine parietal endoderm cells labelled with [35S] ... Full text Link to item Cite

Laminin and epithelial cell attachment.

Journal Article Nature · April 30, 1981 Full text Link to item Cite

Cell interactions and endoderm differentiation in cultured mouse embryos.

Journal Article J Embryol Exp Morphol · April 1981 Morphological and biochemical evidence is presented that the visceral extraembryonic endoderm of the 6.5-day mouse embryo will differentiate into parietal endoderm when cultured in contact with extraembryonic ectoderm undergoing transition into trophoblast ... Link to item Cite

In vitro development of inner cell masses isolated from t0/t0 and tW5/tW5 mouse embryos.

Journal Article J Embryol Exp Morphol · December 1980 Inner cell masses (ICMs) isolated immunosurgically from mouse blastocysts segregating the homozygous lethal mutants t0/t0 and tW5/tW5 were cultured in vitro. Presumed t0/t0 ICMs fail to grow after three days in culture (equivalent gestational day 7.5) when ... Link to item Cite

Development in mammals

Journal Article Nature · December 1, 1978 Full text Cite

In vitro development of inner cell masses isolated immunosurgically from mouse blastocysts. II. Inner cell masses from 3.5- to 4.0-day p.c. blastocysts.

Journal Article J Embryol Exp Morphol · June 1978 This paper describes the development in culture of inner cell masses isolated immunosurgically from C3H/He mouse blastocysts immediately after collection between 3.5 and 4.0 days p.c. By 24--48 h most of the inner cell masses isolated from half-expanded bl ... Link to item Cite

In vitro development of inner cell masses isolated immunosurgically from mouse blastocysts. I. Inner cell masses from 3.5-day p.c. blastocysts incubated for 24 h before immunosurgery.

Journal Article J Embryol Exp Morphol · June 1978 This paper describes the in vitro development of inner cell masses isolated immunosurgically from mouse blastocysts which had been collected on 3.5 days p.c. and then incubated for 24 h. The inner cell masses continue to grow in culture and develop through ... Link to item Cite

Teratocarcinoma cells can develop normally

Journal Article Nature · December 1, 1975 Full text Cite

Effect of protease inhibitors on protein degradation in rat hepatoma cells I. Effect on general protein degradation

Journal Article BBA - General Subjects · December 5, 1974 Tosyllysine chloromethyl ketone and tosylphenylalanine chloromethyl ketone in vitro are active-site specific and irreversible inhibitors of trypsin (EC 3.4.21.4) and chymotrypsin (EC. 3.4.21.1) respectively. Using rat hepatoma cells in suspension culture, ... Full text Cite

Effect of protease inhibitors on protein degradation in rat hepatoma cells II. Effects on ornithine decarboxylase and tyrosine aminotransferase

Journal Article BBA - General Subjects · December 5, 1974 Tyrosine aminotransferase (EC 2.6.1.5) and ornithine decarboxylase (EC 4.1.1.17) are both inducible, pyridoxal phosphate-requiring enzymes, which undergo rapid turnover in cultured rat hepatoma (HTC) cells, as measured by loss of enzyme activity following ... Full text Cite

Control of 3T3 cell growth

Journal Article Nature · December 1, 1974 Full text Cite

The effect of protein synthesis inhibition on the entry of messenger RNA into the cytoplasm of sea urchin embryos.

Journal Article J Cell Biol · June 1971 Emetine is a potent inhibitor of protein synthesis in sea urchin embryos. At a concentration of the drug that rapidly inhibits protein synthesis in blastulae by 95%, uridine incorporation into RNA continues for more than 1 hr and presumptive histone messen ... Full text Link to item Cite