Scholars@Duke
George A. Truskey

George A. Truskey

R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical Engineering
Biomedical Engineering
george.truskey@duke.edu
Box 90281, Durham, NC 27708-0281
1395 Fciemas, 101 Science Drive, Durham, NC 27708-0281

Selected Publications

Interferon-β-Induced Injury During Pediatric Muscle Differentiation: Insight Into Juvenile Dermatomyositis Pathogenesis.

Journal Article ACR Open Rheumatol · October 22, 2024 OBJECTIVE: Juvenile dermatomyositis (JDM) involves up-regulated type I interferons (IFNs), including IFNβ, yet pathologic mechanisms remain poorly understood. We aimed to characterize the functional and structural effects of IFNβ on in vitro human pediatri ... Full text Link to item Cite

Angiopoietin-2 reverses endothelial cell dysfunction in progeria vasculature.

Journal Article Aging cell · October 2024 Hutchinson-Gilford progeria syndrome (HGPS) is a rare premature aging disorder in children caused by a point mutation in the lamin A gene, resulting in a toxic form of lamin A called progerin. Accelerated atherosclerosis leading to heart attack and stroke ... Full text Cite

Genetic changes from type I interferons and JAK inhibitors: clues to drivers of juvenile dermatomyositis.

Journal Article Rheumatology (Oxford) · September 1, 2024 OBJECTIVE: To better understand the pathogenesis of juvenile dermatomyositis (JDM), we examined the effect of the cytokines type I interferons (IFN I) and JAK inhibitor drugs (JAKi) on gene expression in bioengineered pediatric skeletal muscle. METHODS: My ... Full text Link to item Cite

Multi-cellular engineered living systems to assess reproductive toxicology.

Journal Article Reproductive toxicology (Elmsford, N.Y.) · August 2024 Toxicants and some drugs can negatively impact reproductive health. Many toxicants haven't been tested due to lack of available models. The impact of many drugs taken during pregnancy to address maternal health may adversely affect fetal development with l ... Full text Cite

Bioengineered Model of Human LGMD2B Skeletal Muscle Reveals Roles of Intracellular Calcium Overload in Contractile and Metabolic Dysfunction in Dysferlinopathy.

Journal Article Adv Sci (Weinh) · August 2024 Dysferlin is a multi-functional protein that regulates membrane resealing, calcium homeostasis, and lipid metabolism in skeletal muscle. Genetic loss of dysferlin results in limb girdle muscular dystrophy 2B/2R (LGMD2B/2R) and other dysferlinopathies - rar ... Full text Link to item Cite

Ammonia transporter RhBG initiates downstream signaling and functional responses by activating NFκB.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · July 2024 Transceptors, solute transporters that facilitate intracellular entry of molecules and also initiate intracellular signaling events, have been primarily studied in lower-order species. Ammonia, a cytotoxic endogenous metabolite, is converted to urea in hep ... Full text Cite

Effect of type I interferon on engineered pediatric skeletal muscle: a promising model for juvenile dermatomyositis.

Journal Article Rheumatology (Oxford) · January 4, 2024 OBJECTIVE: To investigate pathogenic mechanisms underlying JDM, we defined the effect of type I IFN, IFN-α and IFN-β, on pediatric skeletal muscle function and expression of myositis-related proteins using an in vitro engineered human skeletal muscle model ... Full text Link to item Cite

Mechanotransduction of the vasculature in Hutchinson-Gilford Progeria Syndrome.

Journal Article Frontiers in physiology · January 2024 Hutchinson-Gilford Progeria Syndrome (HGPS) is a premature aging disorder that causes severe cardiovascular disease, resulting in the death of patients in their teenage years. The disease pathology is caused by the accumulation of progerin, a mutated form ... Full text Cite

PCSK9 activation promotes early atherosclerosis in a vascular microphysiological system.

Journal Article APL bioengineering · December 2023 Atherosclerosis is a primary precursor of cardiovascular disease (CVD), the leading cause of death worldwide. While proprotein convertase subtilisin/kexin 9 (PCSK9) contributes to CVD by degrading low-density lipoprotein receptors (LDLR) and altering lipid ... Full text Cite

The Potential of Deep Learning to Advance Clinical Applications of Computational Biomechanics.

Journal Article Bioengineering (Basel, Switzerland) · September 2023 When combined with patient information provided by advanced imaging techniques, computational biomechanics can provide detailed patient-specific information about stresses and strains acting on tissues that can be useful in diagnosing and assessing treatme ... Full text Cite

Lonafarnib and everolimus reduce pathology in iPSC-derived tissue engineered blood vessel model of Hutchinson-Gilford Progeria Syndrome.

Journal Article Scientific reports · March 2023 Hutchinson-Gilford Progeria Syndrome (HGPS) is a rare, fatal genetic disease that accelerates atherosclerosis. With a limited pool of HGPS patients, clinical trials face unique challenges and require reliable preclinical testing. We previously reported a 3 ... Full text Cite

Chemotherapeutic drug screening in 3D-Bioengineered human myobundles provides insight into taxane-induced myotoxicities.

Journal Article iScience · October 21, 2022 Two prominent frontline breast cancer (BC) chemotherapies commonly used in combination, doxorubicin (DOX) and docetaxel (TAX), are associated with long-lasting cardiometabolic and musculoskeletal side effects. Whereas DOX has been linked to mitochondrial d ... Full text Open Access Link to item Cite

Late onset cardiovascular dysfunction in adult mice resulting from galactic cosmic ray exposure.

Journal Article iScience · April 15, 2022 The complex and inaccessible space radiation environment poses an unresolved risk to astronaut cardiovascular health during long-term space exploration missions. To model this risk, healthy male c57BL/6 mice aged six months (corresponding to an astronaut o ... Full text Link to item Cite

Principles for the design of multicellular engineered living systems.

Journal Article APL bioengineering · March 2022 Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engin ... Full text Cite

Tissue engineered skeletal muscle model of rheumatoid arthritis using human primary skeletal muscle cells.

Journal Article J Tissue Eng Regen Med · February 2022 Rheumatoid arthritis (RA) is a chronic inflammatory disease primarily targeting the joints. Autoreactive immune cells involved in RA affect other tissues, including skeletal muscle. Patients with RA experience diminished physical function, limited mobility ... Full text Link to item Cite

In Situ Fabrication and Perfusion of Tissue-Engineered Blood Vessel Microphysiological System.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2022 Human tissue-engineered blood vessels (TEBVs) that exhibit vasoactivity can be used to test drug toxicity, modulate pro-inflammatory cytokines, and model disease states in vitro. We developed a novel device to fabricate arteriole-scale human endothelialize ... Full text Cite

Patient- and Ventilator-Specific Modeling to Drive the Use and Development of 3D Printed Devices for Rapid Ventilator Splitting During the COVID-19 Pandemic

Conference Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) · January 1, 2022 In the early days of the COVID-19 pandemic, there was a pressing need for an expansion of the ventilator capacity in response to the COVID19 pandemic. Reserved for dire situations, ventilator splitting is complex, and has previously been limited to patient ... Full text Cite

Differential microRNA profiles of intramuscular and secreted extracellular vesicles in human tissue-engineered muscle.

Journal Article Front Physiol · 2022 Exercise affects the expression of microRNAs (miR/s) and muscle-derived extracellular vesicles (EVs). To evaluate sarcoplasmic and secreted miR expression in human skeletal muscle in response to exercise-mimetic contractile activity, we utilized a three-di ... Full text Open Access Link to item Cite

Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.

Journal Article STAR protocols · June 2021 Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 mill ... Full text Cite

Biofabrication of tissue engineering vascular systems.

Journal Article APL bioengineering · June 2021 Cardiovascular disease (CVD) is the leading cause of death among persons aged 65 and older in the United States and many other developed countries. Tissue engineered vascular systems (TEVS) can serve as grafts for CVD treatment and be used as in vitro Full text Cite

Emulating Early Atherosclerosis in a Vascular Microphysiological System Using Branched Tissue-Engineered Blood Vessels.

Journal Article Advanced biology · April 2021 Atherosclerosis begins with the accumulation of cholesterol-carrying lipoproteins on blood vessel walls and progresses to endothelial cell dysfunction, monocyte adhesion, and foam cell formation. Endothelialized tissue-engineered blood vessels (TEBVs) have ... Full text Cite

The NIH Somatic Cell Genome Editing program.

Journal Article Nature · April 2021 The move from reading to writing the human genome offers new opportunities to improve human health. The United States National Institutes of Health (NIH) Somatic Cell Genome Editing (SCGE) Consortium aims to accelerate the development of safer and more-eff ... Full text Link to item Cite

Development and Application of Endothelial Cells Derived From Pluripotent Stem Cells in Microphysiological Systems Models.

Journal Article Frontiers in cardiovascular medicine · January 2021 The vascular endothelium is present in all organs and blood vessels, facilitates the exchange of nutrients and waste throughout different organ systems in the body, and sets the tone for healthy vessel function. Mechanosensitive in nature, the endothelium ... Full text Open Access Cite

Glucose Uptake and Insulin Response in Tissue-engineered Human Skeletal Muscle.

Journal Article Tissue engineering and regenerative medicine · December 2020 BackgroundTissue-engineered muscles ("myobundles") offer a promising platform for developing a human in vitro model of healthy and diseased muscle for drug development and testing. Compared to traditional monolayer cultures, myobundles better mode ... Full text Cite

Modeling early stage atherosclerosis in a primary human vascular microphysiological system.

Journal Article Nature communications · October 2020 Novel atherosclerosis models are needed to guide clinical therapy. Here, we report an in vitro model of early atherosclerosis by fabricating and perfusing multi-layer arteriole-scale human tissue-engineered blood vessels (TEBVs) by plastic compression. TEB ... Full text Cite

Application of Oxidative Stress to a Tissue-Engineered Vascular Aging Model Induces Endothelial Cell Senescence and Activation.

Journal Article Cells · May 2020 Clinical studies have established a connection between oxidative stress, aging, and atherogenesis. These factors contribute to senescence and inflammation in the endothelium and significant reductions in endothelium-dependent vasoreactivity in aged patient ... Full text Cite

The future of biomedical engineering: Bioengineering of organoids and tissue development

Journal Article Current Opinion in Biomedical Engineering · March 1, 2020 Full text Cite

iPSC-Derived Endothelial Cells Affect Vascular Function in a Tissue-Engineered Blood Vessel Model of Hutchinson-Gilford Progeria Syndrome.

Journal Article Stem cell reports · February 2020 Hutchinson-Gilford progeria syndrome (HGPS) is a rare disorder caused by a point mutation in the Lamin A gene that produces the protein progerin. Progerin toxicity leads to accelerated aging and death from cardiovascular disease. To elucidate the effects o ... Full text Cite

Human iPSCs Stretch to Improve Tissue-Engineered Vascular Grafts.

Journal Article Cell stem cell · February 2020 Human induced pluripotent stem cells (hiPSCs) provide a potentially unlimited cell source for producing autologous tissue-engineered vascular grafts (TEVGs), which currently suffer from low mechanical strength. In this issue of Cell Stem Cell, Luo et al. ( ... Full text Cite

A novel in situ monitoring system of 3D engineered muscle

Conference 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 · January 1, 2020 In this work, we developed an in situ force monitoring system of skeletal muscle based on “ecoflex-coupling method”. The three dimensional (3D) human skeletal muscle myobundles was fabricated in series with ecoflex (platinum-catalyzed silicones) film conta ... Cite

Modeling statin myopathy in a human skeletal muscle microphysiological system.

Journal Article PLoS One · 2020 Statins are used to lower cholesterol and prevent cardiovascular disease. Musculoskeletal side effects known as statin associated musculoskeletal symptoms (SAMS), are reported in up to 10% of statin users, necessitating statin therapy interruption and incr ... Full text Link to item Cite

Circulating mitochondria in organ donors promote allograft rejection.

Journal Article Am J Transplant · July 2019 The innate immune system is a critical regulator of the adaptive immune responses that lead to allograft rejection. It is increasingly recognized that endogenous molecules released from tissue injury and cell death are potent activators of innate immunity. ... Full text Link to item Cite

Modeling the Effect of TNF-α upon Drug-Induced Toxicity in Human, Tissue-Engineered Myobundles.

Journal Article Ann Biomed Eng · July 2019 A number of significant muscle diseases, such as cachexia, sarcopenia, systemic chronic inflammation, along with inflammatory myopathies share TNF-α-dominated inflammation in their pathogenesis. In addition, inflammatory episodes may increase susceptibilit ... Full text Link to item Cite

Oxygen consumption in human, tissue-engineered myobundles during basal and electrical stimulation conditions.

Journal Article APL bioengineering · June 2019 During three-dimensional culture of skeletal muscle in vitro, electrical stimulation provides an important cue to enhance skeletal muscle mimicry of the in vivo structure and function. However, increased respiration can cause oxygen transport ... Full text Cite

Abstract P284: The Chemotherapeutic Agent Docetaxel Disrupts Mitochondrial Energetics in 3D Human Bioengineered Myobundles

Conference Circulation · March 5, 2019 Taxanes (i.e. docetaxel, (TAX)) comprise the first line-treatment for breast cancer (BC), mostly in combination with anthracyclines like doxorubicin (DOX). The efficacy of these drugs as antineoplastic agents has helped to boost 10-yea ... Full text Cite

The future of biomedical engineering — digital health

Journal Article Current Opinion in Biomedical Engineering · March 1, 2019 Full text Cite

Development and application of human skeletal muscle microphysiological systems.

Journal Article Lab on a chip · October 2018 A number of major disease states involve skeletal muscle, including type 2 diabetes, muscular dystrophy, sarcopenia and cachexia arising from cancer or heart disease. Animals do not accurately represent many of these disease states. Human skeletal muscle m ... Full text Cite

A system to monitor statin-induced myopathy in individual engineered skeletal muscle myobundles.

Journal Article Lab on a chip · September 2018 Microphysiological tissue engineering models of human skeletal muscle (myobundles) provide a platform to investigate the mechanism of muscle diseases and to study the response to drugs and toxins in vitro. To examine the dynamic response to drugs, which of ... Full text Cite

Real-time observation of leukocyte-endothelium interactions in tissue-engineered blood vessel.

Journal Article Lab on a chip · July 2018 Human cell-based 3D tissue constructs play an increasing role in disease modeling and drug screening. Inflammation, atherosclerosis, and many autoimmune disorders involve the interactions between immune cells and blood vessels. However, it has been difficu ... Full text Cite

ECHOCARDIOGRAPHIC MEASUREMENT OF CARDIAC TWIST: A SYSTEMATIC REVIEW AND META-ANALYSIS

Conference Journal of the American College of Cardiology · March 2018 Full text Cite

The future of biomedical engineering – Vascular bioengineering

Journal Article Current Opinion in Biomedical Engineering · March 1, 2018 Full text Cite

Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle.

Journal Article Journal of tissue engineering and regenerative medicine · February 2018 Skeletal muscle holds significant regenerative potential but is incapable of restoring tissue loss caused by severe injury, congenital defects or tumour ablation. Consequently, skeletal muscle models are being developed to study human pathophysiology and r ... Full text Cite

A cardiac patch from aligned microvessel and cardiomyocyte patches.

Journal Article Journal of tissue engineering and regenerative medicine · February 2018 Cardiac tissue engineering aims to produce replacement tissue patches in the lab to replace or treat infarcted myocardium. However, current patches lack preformed microvascularization and are therefore limited in thickness and force production. In this stu ... Full text Cite

Human Microphysiological Systems and Organoids as in Vitro Models for Toxicological Studies.

Journal Article Frontiers in public health · January 2018 Organoids and microphysiological systems represent two current approaches to reproduce organ function in vitro. These systems can potentially provide unbiased assays of function which are needed to understand the mechanism of action of environmental ... Full text Cite

Tissue-Engineered Human Skeletal Muscle Model of Rheumatoid Arthritis

Conference TISSUE ENGINEERING PART A · December 1, 2017 Link to item Cite

In Situ Monitoring of Engineering Muscle to Assess Myopathy

Conference TISSUE ENGINEERING PART A · December 1, 2017 Link to item Cite

Tissue-Engineered Human Skeletal Muscle Model of Rheumatoid Arthritis

Conference ARTHRITIS & RHEUMATOLOGY · October 1, 2017 Link to item Cite

A Tissue Engineered Blood Vessel Model of Hutchinson-Gilford Progeria Syndrome Using Human iPSC-derived Smooth Muscle Cells.

Journal Article Scientific reports · August 2017 Hutchison-Gilford Progeria Syndrome (HGPS) is a rare, accelerated aging disorder caused by nuclear accumulation of progerin, an altered form of the Lamin A gene. The primary cause of death is cardiovascular disease at about 14 years. Loss and dysfunction o ... Full text Cite

Human, Tissue-Engineered, Skeletal Muscle Myobundles to Measure Oxygen Uptake and Assess Mitochondrial Toxicity.

Journal Article Tissue Eng Part C Methods · April 2017 Mitochondrial dysfunction is responsible for the toxicity of a number of drugs. Current isolated mitochondria or cellular monoculture mitochondrial respiration measurement systems lack physiological relevance. Using a tissue engineering rather than cell- o ... Full text Link to item Cite

The future of biomedical engineering

Journal Article Current Opinion in Biomedical Engineering · March 1, 2017 Full text Cite

Functional Coupling of Human Microphysiology Systems: Intestine, Liver, Kidney Proximal Tubule, Blood-Brain Barrier and Skeletal Muscle.

Journal Article Scientific reports · February 2017 Organ interactions resulting from drug, metabolite or xenobiotic transport between organs are key components of human metabolism that impact therapeutic action and toxic side effects. Preclinical animal testing often fails to predict adverse outcomes arisi ... Full text Cite

Hemodynamic Parameters and Early Intimal Thickening in Branching Blood Vessels.

Journal Article Critical reviews in biomedical engineering · January 2017 Intimal thickening due to atherosclerotic lesions or intimal hyperplasia in medium to large blood vessels is a major contributor to heart disease, the leading cause of death in the Western World. Balloon angioplasty with stenting, bypass surgery, and endar ... Full text Cite

Optimizing 3D models of engineered skeletal muscle

Chapter · January 1, 2017 Engineered skeletal muscle tissue can be designed and optimized for the replacement of damaged or diseased skeletal muscle tissue, or serve as an in vitro model of both healthy and disease state skeletal muscle. Such in vitro models can be used to screen d ... Full text Cite

Point-of-care seeding of nitinol stents with blood-derived endothelial cells.

Journal Article J Biomed Mater Res B Appl Biomater · November 2016 Nitinol-based vascular devices, for example, peripheral and intracranial stents, are limited by thrombosis and restenosis. To ameliorate these complications, we developed a technology to promote vessel healing by rapidly seeding (QuickSeeding) autologous b ... Full text Link to item Cite

Cell Density and Joint microRNA-133a and microRNA-696 Inhibition Enhance Differentiation and Contractile Function of Engineered Human Skeletal Muscle Tissues.

Journal Article Tissue Eng Part A · April 2016 To utilize three-dimensional (3D) engineered human skeletal muscle tissue for translational studies and in vitro studies of drug toxicity, there is a need to promote differentiation and functional behavior. In this study, we identified conditions to promot ... Full text Link to item Cite

Transdifferentiation of human endothelial progenitors into smooth muscle cells.

Journal Article Biomaterials · April 2016 Access to smooth muscle cells (SMC) would create opportunities for tissue engineering, drug testing, and disease modeling. Herein we report the direct conversion of human endothelial progenitor cells (EPC) to induced smooth muscle cells (iSMC) by induced e ... Full text Cite

Poly(ethylene glycol) Hydrogel Scaffolds Containing Cell-Adhesive and Protease-Sensitive Peptides Support Microvessel Formation by Endothelial Progenitor Cells.

Journal Article Cellular and molecular bioengineering · March 2016 The development of stable, functional microvessels remains an important obstacle to overcome for tissue engineered organs and treatment of ischemia. Endothelial progenitor cells (EPCs) are a promising cell source for vascular tissue engineering as they are ... Full text Cite

Human Vascular Microphysiological System for in vitro Drug Screening.

Journal Article Sci Rep · February 18, 2016 In vitro human tissue engineered human blood vessels (TEBV) that exhibit vasoactivity can be used to test human toxicity of pharmaceutical drug candidates prior to pre-clinical animal studies. TEBVs with 400-800 μM diameters were made by embedding human ne ... Full text Open Access Link to item Cite

Point-of-Care Rapid-Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating.

Journal Article ASAIO J · 2016 The most promising alternatives to heart transplantation are left ventricular assist devices and artificial hearts; however, their use has been limited by thrombotic complications. To reduce these, sintered titanium (Ti) surfaces were developed, but thromb ... Full text Link to item Cite

Advancing cardiovascular tissue engineering.

Journal Article F1000Research · January 2016 Cardiovascular tissue engineering offers the promise of biologically based repair of injured and damaged blood vessels, valves, and cardiac tissue. Major advances in cardiovascular tissue engineering over the past few years involve improved methods to prom ... Full text Open Access Cite

Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.

Journal Article Sci Rep · October 12, 2015 Tissue-engineered blood vessels (TEBV) can serve as vascular grafts and may also play an important role in the development of organs-on-a-chip. Most TEBV construction involves scaffolding with biomaterials such as collagen gel or electrospun fibrous mesh. ... Full text Open Access Link to item Cite

Umbilical Cord Blood-Derived Mononuclear Cells Exhibit Pericyte-Like Phenotype and Support Network Formation of Endothelial Progenitor Cells In Vitro.

Journal Article Annals of biomedical engineering · October 2015 Umbilical cord blood represents a promising cell source for pro-angiogenic therapies. The present study examined the potential of mononuclear cells (MNCs) from umbilical cord blood to support endothelial progenitor cell (EPC) microvessel formation. MNCs we ... Full text Cite

Human Tissue Engineered Blood Vessels for in vitro Drug Testing

Conference TISSUE ENGINEERING PART A · September 1, 2015 Link to item Cite

Novel In Vitro Exercise Model of Engineered Human Skeletal Muscle

Conference TISSUE ENGINEERING PART A · September 1, 2015 Link to item Cite

Tissue-engineered blood vessels as promising tools for testing drug toxicity.

Journal Article Expert opinion on drug metabolism & toxicology · July 2015 Drug-induced vascular injury (DIVI) is a serious problem in preclinical studies of vasoactive molecules and for survivors of pediatric cancers. DIVI is often observed in rodents and some larger animals, primarily with drugs affecting vascular tone, but not ... Full text Cite

Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering.

Journal Article Regenerative medicine · May 2015 AimPeripheral blood-derived endothelial cells (pBD-ECs) are an attractive tool for cell therapies and tissue engineering, but have been limited by their low isolation yield. We increase pBD-EC yield via administration of the chemokine receptor typ ... Full text Cite

Endothelial Cell Senescence Increases Traction Forces due to Age-Associated Changes in the Glycocalyx and SIRT1.

Journal Article Cellular and molecular bioengineering · March 2015 Endothelial cell (EC) aging and senescence are key events in atherogenesis and cardiovascular disease development. Age-associated changes in the local mechanical environment of blood vessels have also been linked to atherosclerosis. However, the extent to ... Full text Cite

Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs.

Journal Article Elife · January 9, 2015 Existing in vitro models of human skeletal muscle cannot recapitulate the organization and function of native muscle, limiting their use in physiological and pharmacological studies. Here, we demonstrate engineering of electrically and chemically responsiv ... Full text Open Access Link to item Cite

CD45+ Cells Present Within Mesenchymal Stem Cell Populations Affect Network Formation of Blood-Derived Endothelial Outgrowth Cells.

Journal Article BioResearch open access · January 2015 Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) represent promising cell sources for angiogenic therapies. There are, however, conflicting reports regarding the ability of MSCs to support network formation of endothelial cells. The go ... Full text Open Access Cite

Environmental factors that influence the response of the endothelium to flow

Chapter · January 1, 2015 Complications from cardiovascular disease, such as heart attack and stroke, represent the leading cause of death in the United States and many developed and developing countries. In 2008, approximately 672,000 people died of heart disease or stroke, which ... Full text Cite

Physiology and metabolism of tissue-engineered skeletal muscle.

Journal Article Experimental biology and medicine (Maywood, N.J.) · September 2014 Skeletal muscle is a major target for tissue engineering, given its relative size in the body, fraction of cardiac output that passes through muscle beds, as well as its key role in energy metabolism and diabetes, and the need for therapies for muscle dise ... Full text Cite

Conditions that promote primary human skeletal myoblast culture and muscle differentiation in vitro.

Journal Article Am J Physiol Cell Physiol · February 15, 2014 Conditions under which skeletal myoblasts are cultured in vitro are critical to growth and differentiation of these cells into mature skeletal myofibers. We examined several culture conditions that promoted human skeletal myoblast (HSkM) culture and examin ... Full text Link to item Cite

Magnetoactive sponges for dynamic control of microfluidic flow patterns in microphysiological systems.

Journal Article Lab on a chip · February 2014 We developed a microfluidic flow-control system capable of dynamically generating various flow patterns on demand. The flow-control system is based on novel magnetoactive sponges embedded in microfluidic flow channels. Applying a non-uniform magnetic field ... Full text Cite

Biological and engineering design considerations for vascular tissue engineered blood vessels (TEBVs).

Journal Article Current opinion in chemical engineering · February 2014 Considerable advances have occurred in the development of tissue-engineered blood vessels (TEBVs) to repair or replace injured blood vessels, or as in vitro systems for drug toxicity testing. Here we summarize approaches to produce TEBVs and review ... Full text Cite

Late-outgrowth endothelial progenitors from patients with coronary artery disease: endothelialization of confluent stromal cell layers.

Journal Article Acta biomaterialia · February 2014 Patients with coronary artery disease (CAD) are the primary candidates to receive small-diameter tissue-engineered blood vessels (TEBVs). Peripheral blood derived endothelial progenitor cells (EPCs) from CAD patients (CAD EPCs) represent a minimally invasi ... Full text Cite

Aging endothelial cells exhibit decreased response to atheroprotective shear stress

Journal Article ASME 2013 Summer Bioengineering Conference, SBC 2013 · December 1, 2013 Full text Cite

Surface projections of titanium substrates increase antithrombotic endothelial function in response to shear stress.

Journal Article Journal of biomedical materials research. Part A · November 2013 Despite the therapeutic benefits of both mechanical circulatory assist devices and nitinol stents with titanium (Ti) outer surfaces, problems remain with thrombosis at the blood-contacting surface. Covering these surfaces with a layer of endothelium would ... Full text Cite

Isolation of functional human endothelial cells from small volumes of umbilical cord blood.

Journal Article Annals of biomedical engineering · October 2013 Endothelial cells (ECs) isolated from endothelial progenitor cells in blood have great potential as a therapeutic tool to promote vasculogenesis and angiogenesis and treat cardiovascular diseases. However, current methods to isolate ECs are limited by a lo ... Full text Cite

Endothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose.

Journal Article Cancer Invest · June 2013 Identification of measurable nontransient responses to low-dose radiation in human primary cell cultures remains a problem. To this end, circulating endothelial colony-forming (progenitor) cells (ECFCs) were examined as an experimental model. ECFCs were is ... Full text Link to item Cite

The Effect of Stress-Induced Senescence on Aging Human Cord Blood-Derived Endothelial Cells.

Journal Article Cardiovascular engineering and technology · June 2013 PurposeWe sought to determine the effect of stress-induced senescence on the permeability to albumin of aging endothelial progenitor cells.MethodsHuman umbilical cord blood derived endothelial cells (hCB-ECs) and human aortic endothelial ... Full text Cite

Comparison of mixed and lamellar coculture spatial arrangements for tissue engineering capillary networks in vitro.

Journal Article Tissue engineering. Part A · March 2013 Coculture of endothelial cells (ECs) and smooth muscle cells (SMCs) in vitro can yield confluent monolayers or EC networks. Factors influencing this transition are not known. In this study, we examined whether the spatial arrangement of EC-SMC cocultures a ... Full text Cite

Design considerations for an integrated microphysiological muscle tissue for drug and tissue toxicity testing.

Journal Article Stem Cell Res Ther · 2013 Microphysiological systems provide a tool to simulate normal and pathological function of organs for prolonged periods. These systems must incorporate the key functions of the individual organs and enable interactions among the corresponding microphysiolog ... Full text Link to item Cite

Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration.

Journal Article PloS one · January 2013 The issue of how contractility and adhesion are related to cell shape and migration pattern remains largely unresolved. In this paper we report that Gleevec (Imatinib), an Abl family kinase inhibitor, produces a profound change in the shape and migration o ... Full text Cite

Viscoelastic Cell Adhesion Model (VECAM)

Conference Biophysical Journal · January 2013 Full text Cite

Effect of cellular senescence on the albumin permeability of blood-derived endothelial cells.

Journal Article American journal of physiology. Heart and circulatory physiology · December 2012 In this study, we tested the hypotheses that endothelial cells (ECs) derived from human umbilical cord blood (hCB-ECs) exhibit low permeability, which increases as hCB-ECs age and undergo senescence, and that the change in the permeability of hCB-ECs is du ... Full text Cite

Dynamic quantitative microscopy and nanoscopy of red blood cells in sickle cell disease

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 16, 2012 We have applied wide-field digital interferometric techniques to quantitatively image sickle red blood cells (RBCs) [1] in a noncontact label-free manner, and measure the nanometer-scale fluctuations in their thickness as an indication of their stiffness. ... Full text Cite

Leukocyte rolling on P-selectin: a three-dimensional numerical study of the effect of cytoplasmic viscosity.

Journal Article Biophysical journal · April 2012 Rolling leukocytes deform and show a large area of contact with endothelium under physiological flow conditions. We studied the effect of cytoplasmic viscosity on leukocyte rolling using our three-dimensional numerical algorithm that treats leukocyte as a ... Full text Cite

Computational fluid dynamics analysis to determine shear stresses and rates in a centrifugal left ventricular assist device.

Journal Article Artificial organs · April 2012 Axial flow left ventricular assist devices (LVADs) are a significant improvement in mechanical circulatory support. However, patients with these devices experience degradation of large von Willebrand factor (vWF) multimers, which is associated with bleedin ... Full text Cite

Parallel-plate flow chamber and continuous flow circuit to evaluate endothelial progenitor cells under laminar flow shear stress.

Journal Article J Vis Exp · January 17, 2012 The overall goal of this method is to describe a technique to subject adherent cells to laminar flow conditions and evaluate their response to well quantifiable fluid shear stresses. Our flow chamber design and flow circuit (Fig. 1) contains a transparent ... Full text Link to item Cite

Novel optical signature for sickle cell trait red blood cells

Journal Article Frontiers in Optics, FIO 2012 · January 1, 2012 We identified unique optical signatures for sickle cell trait, a condition where heterozygous individuals are carriers for the hemoglobin allele that causes sickle cell anemia, by using wide-field interferometric microscopy. © OSA 2012. ... Full text Cite

Endothelial progenitor cells for vascular repair

Chapter · December 1, 2011 Endothelial progenitor cells (EPCs), present in the blood and bone marrow, represent a potential source of endothelial cells for repair of injured blood vessels, neovascularization, and tissue engineering. EPCs are present at low levels in peripheral blood ... Full text Cite

Use of autologous blood-derived endothelial progenitor cells at point-of-care to protect against implant thrombosis in a large animal model.

Journal Article Biomaterials · November 2011 Titanium (Ti) is commonly utilized in many cardiovascular devices, e.g. as a component of Nitinol stents, intra- and extracorporeal mechanical circulatory assist devices, but is associated with the risk of thromboemboli formation. We propose to solve this ... Full text Link to item Cite

Autologous endothelial progenitor cell-seeding technology and biocompatibility testing for cardiovascular devices in large animal model.

Journal Article J Vis Exp · September 9, 2011 Implantable cardiovascular devices are manufactured from artificial materials (e.g. titanium (Ti), expanded polytetrafluoroethylene), which pose the risk of thromboemboli formation. We have developed a method to line the inside surface of Ti tubes with aut ... Full text Link to item Cite

Biomechanical effects of flow and coculture on human aortic and cord blood-derived endothelial cells.

Journal Article Journal of biomechanics · July 2011 Human endothelial cells derived from umbilical cord blood (hCB-ECs) represent a promising cell source for endothelialization of tissue engineered blood vessels. hCB-ECs cultured directly above human aortic smooth muscle cells (SMCs), which model native and ... Full text Cite

Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry.

Journal Article J Biomed Opt · March 2011 We have applied wide-field digital interferometry (WFDI) to examine the morphology and dynamics of live red blood cells (RBCs) from individuals who suffer from sickle cell anemia (SCA), a genetic disorder that affects the structure and mechanical propertie ... Full text Link to item Cite

The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti implants.

Journal Article Biomaterials · January 2011 Implantable and extracorporeal cardiovascular devices are commonly made from titanium (Ti) (e.g. Ti-coated Nitinol stents and mechanical circulatory assist devices). Endothelializing the blood-contacting Ti surfaces of these devices would provide them with ... Full text Link to item Cite

Autologous endothelial progenitor cell-seeding technology and biocompatibility testing for cardiovascular devices in large animal model

Journal Article Journal of Visualized Experiments · January 1, 2011 Implantable cardiovascular devices are manufactured from artificial materials (e.g. titanium (Ti), expanded polytetrafluoroethylene), which pose the risk of thromboemboli formation. We have developed a method to line the inside surface of Ti tubes with aut ... Full text Cite

Effect of microRNA modulation on bioartificial muscle function.

Journal Article Tissue Eng Part A · December 2010 Cellular therapies have recently employed the use of small RNA molecules, particularly microRNAs (miRNAs), to regulate various cellular processes that may be altered in disease states. In this study, we examined the effect of transient muscle-specific miRN ... Full text Open Access Link to item Cite

Dynamic adhesion of umbilical cord blood endothelial progenitor cells under laminar shear stress.

Journal Article Biophysical journal · December 2010 Late outgrowth endothelial progenitor cells (EPCs) represent a promising cell source for rapid reendothelialization of damaged vasculature after expansion ex vivo and injection into the bloodstream. We characterized the dynamic adhesion of umbilical-cord-b ... Full text Cite

Human umbilical cord blood-derived endothelial cells reendothelialize vein grafts and prevent thrombosis.

Journal Article Arterioscler Thromb Vasc Biol · November 2010 OBJECTIVE: To accelerate vein graft reendothelialization and reduce vein graft thrombosis by infusing human umbilical cord blood-derived endothelial cells (hCB-ECs) because loss of endothelium contributes to vein graft thrombosis and neointimal hyperplasia ... Full text Open Access Link to item Cite

Endothelial Cell Vascular Smooth Muscle Cell Co-Culture Assay For High Throughput Screening Assays For Discovery of Anti-Angiogenesis Agents and Other Therapeutic Molecules.

Journal Article International journal of high throughput screening · October 2010 Drug development for many diseases would be aided greatly by accurate in vitro model systems that replicate key elements of in vivo physiology. The recent development of co-culture systems of endothelial cells and vascular smooth muscle cells can be extend ... Full text Cite

Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-alpha-mediated endothelial cell activation.

Journal Article American journal of physiology. Heart and circulatory physiology · August 2010 We used a direct-contact endothelial cell-smooth muscle cell (EC-SMC) co-culture to examine whether quiescent SMCs regulate the EC inflammatory response to tumor necrosis factor (TNF)-alpha. ECs were cultured under static and physiological flow conditions. ... Full text Cite

Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials.

Journal Article Ann Biomed Eng · June 2010 Resorbable scaffolds such as polyglycolic acid (PGA) are employed in a number of clinical and tissue engineering applications owing to their desirable property of allowing remodeling to form native tissue over time. However, native PGA does not promote end ... Full text Open Access Link to item Cite

Porcine endothelial cells cocultured with smooth muscle cells became procoagulant in vitro.

Journal Article Tissue engineering. Part A · June 2010 Endothelial cell (EC) seeding represents a promising approach to provide a nonthrombogenic surface on vascular grafts. In this study, we used a porcine EC/smooth muscle cell (SMC) coculture model that was previously developed to examine the efficacy of EC ... Full text Open Access Cite

Comparison of endothelial cell phenotypic markers of late-outgrowth endothelial progenitor cells isolated from patients with coronary artery disease and healthy volunteers.

Journal Article Tissue Eng Part A · November 2009 The lack of easily isolated autologous endothelial cell (EC) sources is one of the major challenges with vascular tissue engineering interventions. This article examines the isolation and expansion of late-outgrowth endothelial progenitor cells (EPCs) from ... Full text Link to item Cite

Characterization of umbilical cord blood-derived late outgrowth endothelial progenitor cells exposed to laminar shear stress.

Journal Article Tissue engineering. Part A · November 2009 Endothelial progenitor cells isolated from umbilical cord blood (CB-EPCs) represent a promising source of endothelial cells for synthetic vascular grafts and tissue-engineered blood vessels since they are readily attainable, can be easily isolated, and pos ... Full text Cite

Mice lacking Homer 1 exhibit a skeletal myopathy characterized by abnormal transient receptor potential channel activity.

Journal Article Mol Cell Biol · April 2008 Transient receptor potential (TRP) channels are nonselective cation channels, several of which are expressed in striated muscle. Because the scaffolding protein Homer 1 has been implicated in TRP channel regulation, we hypothesized that Homer proteins play ... Full text Link to item Cite

Streptavidin binding and endothelial cell adhesion to biotinylated fibronectin.

Journal Article Langmuir : the ACS journal of surfaces and colloids · December 2007 A dual ligand (DL) system that combines high affinity streptavidin-biotin binding with lower affinity fibronectin-integrin ligand binding was developed to augment endothelial cell adhesion to polymers. In this study, we examined the utility of biotinylated ... Full text Cite

The use of mild trypsinization conditions in the detachment of endothelial cells to promote subsequent endothelialization on synthetic surfaces.

Journal Article Biomaterials · September 2007 A necessary condition for endothelialization of small diameter grafts is rapid and firm adhesion of endothelial cells upon exposure to flow. To retain integrins on the cell surface, we assessed the effects of trypsin concentration, the duration of trypsin ... Full text Cite

Smooth muscle cell rigidity and extracellular matrix organization influence endothelial cell spreading and adhesion formation in coculture.

Journal Article American journal of physiology. Heart and circulatory physiology · September 2007 Efforts to develop functional tissue-engineered blood vessels have focused on improving the strength and mechanical properties of the vessel wall, while the functional status of the endothelium within these vessels has received less attention. Endothelial ... Full text Cite

Morphology and ultrastructure of differentiating three-dimensional mammalian skeletal muscle in a collagen gel.

Journal Article Muscle Nerve · July 2007 Because previous studies of three-dimensional skeletal muscle cultures have shown limited differentiation, the goal of this study was to establish conditions that would produce mature sarcomeres in a mammalian-derived skeletal muscle construct. We evaluate ... Full text Link to item Cite

Flow and high affinity binding affect the elastic modulus of the nucleus, cell body and the stress fibers of endothelial cells.

Journal Article Annals of biomedical engineering · July 2007 Cell mechanical properties are important in the adhesion of endothelial cells to synthetic vascular grafts exposed to shear flow. We hypothesized that the local apparent elastic modulus of the nucleus and the cell body would increase to a greater extent fo ... Full text Cite

Effect of cyclic stretch on beta1D-integrin expression and activation of FAK and RhoA.

Journal Article Am J Physiol Cell Physiol · June 2007 Integrins play a pivotal role in proliferation, differentiation, and survival in skeletal and cardiac myocytes. The beta(1D)-isoform of the beta(1)-integrin is specifically expressed in striated skeletal muscle. However, little is known about the role and ... Full text Link to item Cite

Adhesion and function of human endothelial cells co-cultured on smooth muscle cells.

Journal Article Annals of biomedical engineering · March 2007 To evaluate interactions between human endothelial cells (ECs) and smooth muscle cells (SMCs) for the development of tissue-engineered vessels, we examined the adhesion and key cell properties of human ECs grown on quiescent human aortic SMCs. ECs attached ... Full text Cite

Three-dimensional computational modeling of leukocyte rolling and adhesion

Conference 2006 ASME Joint U.S.-European Fluids Engineering Summer Meeting, FEDSM 2006 · December 1, 2006 Recruitment of leukocytes into sites of acute and chronic inflammation is a vital component of the innate immune response in humans and plays an important role in cardiovascular diseases, such as ischemia-reperfusion injury and atherosclerosis. Leukocytes ... Cite

Vascular and Capillary Endothelium

Journal Article · April 14, 2006 AbstractThe endothelium is a monolayer of cells that line the inner lumen of all blood vessels. Endothelial cells respond to both mechanical forces created by blood flow and stimulatory molecules within the blood ... Full text Cite

Normal and shear stresses influence the spatial distribution of intracellular adhesion molecule-1 expression in human umbilical vein endothelial cells exposed to sudden expansion flow.

Journal Article Journal of biomechanics · January 2006 Patterns in cell adhesion molecule expression by endothelial cells may play a role in atherogenesis. Previous studies have shown dependence of intracellular adhesion molecule-1 (ICAM-1) expression in human umbilical vein endothelial cells (HUVEC) on shear ... Full text Cite

Three-dimensional computational modeling of leukocyte rolling and adhesion

Conference Proceedings of ASME Fluids Engineering Division Summer Meeting 2006, FEDSM2006 · January 1, 2006 Recruitment of leukocytes into sites of acute and chronic inflammation is a vital component of the innate immune response in humans and plays an important role in cardiovascular diseases, such as ischemia-reperfusion injury and atherosclerosis. Leukocytes ... Full text Cite

Mylar and Teflon-AF as cell culture substrates for studying endothelial cell adhesion.

Journal Article Biomaterials · December 2005 The textured and opaque nature of Dacron and ePTFE has prevented the use of these fabrics in conventional cell culture techniques normally employed to optimize cell attachment and retention. This lack of optimization has led, in part, to the poor performan ... Full text Cite

A system for the direct co-culture of endothelium on smooth muscle cells.

Journal Article Biomaterials · August 2005 The development of a functional, adherent endothelium is one of the major factors limiting the successful development of tissue engineered vascular grafts (TEVGs). The adhesion and function of endothelial cells (ECs) on smooth muscle cells (SMCs) are poorl ... Full text Cite

Effects of titanium particle size on osteoblast functions in vitro and in vivo.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · March 2005 The formation of titanium (Ti)-wear particles during the lifetime of an implant is believed to be a major component of loosening due to debris-induced changes in bone cell function. Radiographic evidence indicates a loss of fixation at the implant-bone int ... Full text Cite

In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts.

Journal Article J Biomed Mater Res B Appl Biomater · January 15, 2005 In this study, we examined combinations of three approaches to improve the adhesion of endothelial cells (EC) onto expanded polytetrafluoroethylene (ePTFE) vascular grafts placed at the femoral artery of rats: (1) high-affinity receptor-ligand binding of R ... Full text Link to item Cite

Three-dimensional numerical simulation of receptor-mediated leukocyte adhesion to surfaces: Effects of cell deformability and viscoelasticity

Journal Article Physics of Fluids · January 1, 2005 Computational fluid dynamics is used to investigate the effects of cell deformability and viscoelasticity on receptor-mediated leukocyte adhesion to endothelium or a ligand coated surface in a parallel-plate flow chamber. In the three-dimensional numerical ... Full text Cite

Synergistic effect of shear stress and streptavidin-biotin on the expression of endothelial vasodilator and cytoskeleton genes.

Journal Article Biotechnology and bioengineering · December 2004 Dual ligand treatment of streptavidin(SA)-biotin and fibronectin (Fn) enhances the adhesion of endothelial cells (EC) onto synthetic surfaces and promotes the quiescent phenotype of adherent EC. The current study investigates the effect of the dual ligand ... Full text Cite

Linoleic acid increases monocyte deformation and adhesion to endothelium.

Journal Article Atherosclerosis · December 2004 Fatty acids have been implicated in having both anti- or pro-inflammatory actions, which may contribute to the progression and severity of atherosclerosis. Linoleic acid has been shown by others to decrease CD18 expression and leukocyte adhesion under stat ... Full text Cite

A 3D numerical study of the effect of channel height on leukocyte deformation and adhesion in parallel-plate flow chambers.

Journal Article Microvascular research · November 2004 The effect of channel height on leukocyte adhesion to a lower plate in a parallel-plate flow chamber is studied by direct numerical simulations in three dimensions. The numerical model takes into account deformability and viscoelasticity of the leukocyte, ... Full text Cite

Stretch-induced nitric oxide modulates mechanical properties of skeletal muscle cells.

Journal Article Am J Physiol Cell Physiol · August 2004 In this study, we examined the hypothesis that stretch-induced (nitric oxide) NO modulates the mechanical properties of skeletal muscles by increasing accumulation of protein levels of talin and vinculin and by inhibiting calpain-induced proteolysis, there ... Full text Link to item Cite

Effect of streptavidin-biotin on endothelial vasoregulation and leukocyte adhesion.

Journal Article Biomaterials · August 2004 The current study examines whether the adhesion promoting arginine-glycine-aspartate-streptavidin mutant (RGD-SA) also affects two important endothelial cell (EC) functions in vitro: vasoregulation and leukocyte adhesion. EC adherent to surfaces via fibron ... Full text Cite

Effect of streptavidin RGD mutant on the adhesion of endothelial cells.

Journal Article Biotechnology progress · March 2004 Adhesion of endothelial cells (EC) to surfaces can be enhanced by supplementing the integrin-mediated adhesion with high-affinity streptavidin (SA) that links a biotinylated EC to a biotinylated surface. Biotin pullout from the EC membrane limits the effec ... Full text Cite

Real-time theoretical compartmental model of blood-brain barrier drug delivery.

Conference Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference · January 2004 This study developed upon the Mahar Doan and Boje simulations to create a novel pharmacokinetic/pharmacodynamic real-time compartmental model in Simulink. The model allowed for varying combinations of oral and intravenous drug dosing under steady and nonst ... Full text Cite

High-affinity augmentation of endothelial cell attachment: long-term effects on focal contact and actin filament formation.

Journal Article Journal of biomedical materials research. Part A · September 2003 Coadsorption of high-affinity avidin with lower affinity cell adhesion protein fibronectin has been shown to significantly augment short-term (1 h) adhesion and spreading of endothelial cells; however, the longer term persistence of avidin binding and its ... Full text Cite

Hemodynamics simulation and identification of susceptible sites of atherosclerotic lesion formation in a model abdominal aorta.

Journal Article Journal of biomechanics · August 2003 Employing the rabbit's abdominal aorta as a suitable atherosclerotic model, transient three-dimensional blood flow simulations and monocyte deposition patterns were used to evaluate the following hypotheses: (i) simulation of monocyte transport through a m ... Full text Cite

Effect of streptavidin affinity mutants on the integrin-independent adhesion of biotinylated endothelial cells.

Journal Article Biomaterials · February 2003 We have previously shown that the high-affinity streptavidin (SA)-biotin interaction enhanced the initial integrin-mediated adhesion of biotinylated endothelial cells to SA-coated surface by serving as an extrinsic bond to stabilize and enhance the intrins ... Full text Cite

Relation between near-wall residence times of monocytes and early lesion growth in the rabbit aorto-celiac junction.

Journal Article Annals of biomedical engineering · January 2003 Transient particle-hemodynamic simulations were conducted in a model of the rabbit aorto-celiac junction to investigate mechanisms responsible for localized monocyte attachment and subsequent lesion formation. We hypothesized that the probability for monoc ... Full text Cite

Synergistic effect of high-affinity binding and flow preconditioning on endothelial cell adhesion.

Journal Article Journal of biomedical materials research. Part A · January 2003 The current study examined whether the combined introduction of high-affinity avidin-biotin bonds and fibronectin-integrin bonds (i.e., dual ligand treatment) would further augment the adhesion of flow-preconditioned endothelial cells to model substrates v ... Full text Cite

The effects of the actin cytoskeleton on the transverse mechanical properties of skeletal muscle cells

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 2002 This study examined the contributions of actin filaments and actomyosin cytoskeleton to the transverse mechanical properties of skeletal muscle cells. The study is motivated by the goal of understanding the molecular mechanism behind skeletal myoblast tran ... Cite

The effects of streptavidin-biotin exogenous ligands on the endothelium-derived nitric oxide synthase activity

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 2002 In this study, the effects of the streptavidin(SA)-biotin ligands on the amount of endothelium-derived nitric oxide synthase (eNOS) were quantified. The higher-affinity SA-biotin ligands were previously introduced to help promote the initial endothelial ce ... Cite

Increased numbers of bonds stabilize adhesion with multiple tethers between endothelium and monocytes

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 2002 This investigation explores the hypothesis that increased receptor density promotes the formation of tethers. Micropipet aspiration was used to produce defined normal forces during contact and detachment between monocytes and human umbilical vein endotheli ... Cite

Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation.

Journal Article Am J Physiol Cell Physiol · October 2002 The effect of differentiation on the transverse mechanical properties of mammalian myocytes was determined by using atomic force microscopy. The apparent elastic modulus increased from 11.5 +/- 1.3 kPa for undifferentiated myoblasts to 45.3 +/- 4.0 kPa aft ... Full text Link to item Cite

Effect of fluid shear stress on the permeability of the arterial endothelium.

Journal Article Annals of biomedical engineering · April 2002 The localization of atherosclerotic lesions is due, in part, to regional variations in the permeability of arterial endothelium to macromolecules. In turn, endothelial permeability may be influenced by fluid shear stresses. The spatial variation in endothe ... Full text Cite

Factors influencing the nonuniform localization of monocytes in the arterial wall.

Journal Article Biorheology · January 2002 Adhesion of monocytes to arterial endothelium may contribute to the asymmetric distribution of atherosclerotic lesions. Possible mechanisms for adhesion in the relatively high shear stress environment found in arteries include greater monocyte deformation ... Cite

Endothelial, cardiac muscle and skeletal muscle exhibit different viscous and elastic properties as determined by atomic force microscopy.

Journal Article J Biomech · December 2001 This study evaluated the hypothesis that, due to functional and structural differences, the apparent elastic modulus and viscous behavior of cardiac and skeletal muscle and vascular endothelium would differ. To accurately determine the elastic modulus, the ... Full text Link to item Cite

The lagrangian characterization of resting flow in the rabbit abdominal aorta

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · December 1, 2001 The lagrangian characterization of resting flow in the rabbit abdominal aorta was investigated. The flow fields were computed by using a validated and user-enhanced control volume code. It was found that the pathlines released during the systolic phase rev ... Cite

Relationship between LDL residence time and macrophage accumulation in the rabbit aorta

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · December 1, 2001 The relationship between low density lipoprotein (LDL) residence time and macrophage accumulation in the rabbit aorta was studied. It was hypothesized that longer LDL residence times increased the likelihood for modification of LDL. It was found that for r ... Cite

Influence of non-planar curvature at the rabbit aorto-celiac junction

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · December 1, 2001 Influence of non-planar curvature at the rabbit aorto-celiac junction was investigated. Quantitative and qualitative comparisons were also performed between time-averaged hemodynamic wall parameters and early disease processes. The different mixing charact ... Cite

Effect of contact time and force on monocyte adhesion to vascular endothelium.

Journal Article Biophysical journal · April 2001 In this study we examined whether monocytic cell attachment to vascular endothelium was affected by elevating shear stress at a constant shear rate. Contact time, which is inversely related to the shear rate, was fixed and viscosity elevated with dextran t ... Full text Cite

Effects of chronic exposure to simulated microgravity on skeletal muscle cell proliferation and differentiation.

Journal Article In Vitro Cell Dev Biol Anim · March 2001 Cell culture models that mimic long-term exposure to microgravity provide important insights into the cellular biological adaptations of human skeletal muscle to long-term residence in space. We developed insert scaffolding for the NASA-designed rotating c ... Full text Link to item Cite

Hemodynamic parameters and early intimal thickening in branching blood vessels.

Journal Article Critical reviews in biomedical engineering · January 2001 Intimal thickening due to atherosclerotic lesions or intimal hyperplasia in medium to large blood vessels is a major contributor to heart disease, the leading cause of death in the Western World. Balloon angioplasty with stenting, bypass surgery, and endar ... Full text Cite

Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells

Journal Article Annals of Biomedical Engineering · December 1, 2000 Atomic force microscopy (AFM) and total internal reflection fluorescence microscopy (TIRFM) were configured to examine the transmission of force applied from the apical cell membrane to the basal cell membrane of HUVECs. The precise localized mechanical pe ... Cite

Mechanism of shear force-dependent monocyte adhesion to vascular endothelium

Journal Article Annals of Biomedical Engineering · December 1, 2000 As fluid shear stress and hence net force on cells increases at a constant shear rate (contact time), monocyte adhesion increases. This phenomena is hypothesized to result from penetration of charge barriers and increased deformation of the monocytic cell ... Cite

Orientation and length of mammalian skeletal myocytes in response to a unidirectional stretch.

Journal Article Cell Tissue Res · November 2000 Effects of mechanical forces exerted on mammalian skeletal muscle cells during development were studied using an in vitro model to unidirectionally stretch cultured C2C12 cells grown on silastic membrane. Previous models to date have not studied these resp ... Full text Link to item Cite

Differentiation of mammalian skeletal muscle cells cultured on microcarrier beads in a rotating cell culture system.

Journal Article Med Biol Eng Comput · September 2000 The growth and repair of adult skeletal muscle are due in part to activation of muscle precursor cells, commonly known as satellite cells or myoblasts. These cells are responsive to a variety of environmental cues, including mechanical stimuli. The overall ... Full text Link to item Cite

Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells.

Journal Article Biophysical journal · April 2000 This paper describes the combined use of atomic force microscopy (AFM) and total internal reflection fluorescence microscopy (TIRFM) to examine the transmission of force from the apical cell membrane to the basal cell membrane. A Bioscope AFM was mounted o ... Full text Cite

Mechanism of shear force-dependent monocyte adhesion to vascular endothelium

Journal Article Annals of Biomedical Engineering · 2000 As fluid shear stress and hence net force on cells increases at a constant shear rate (contact time), monocyte adhesion increases. This phenomena is hypothesized to result from penetration of charge barriers and increased deformation of the monocytic cell ... Cite

Total internal reflection microscopy and atomic force microscopy (TIRFM-AFM) to study stress transduction mechanisms in endothelial cells.

Journal Article Critical reviews in biomedical engineering · January 2000 The cytoskeleton plays a key role in providing strength and structure to the cell. A force balance exists between the cytoskeleton and the extracellular matrix/substratum via the focal contact regions. The purpose of this study is to integrate atomic force ... Full text Cite

Role of endothelial cell-substrate contact area and fibronectin-receptor affinity in cell adhesion to HEMA/EMA copolymers.

Journal Article Journal of biomedical materials research · December 1999 The objective of this study was to examine the effect of substrate hydrophobicity on cell-substrate contact area and the affinity between adsorbed fibronectin (Fn) and its receptor. Homo- and copolymer films of hydrophobic ethyl methacrylate (EMA) and hydr ... Full text Cite

An equilibrium model of endothelial cell adhesion via integrin-dependent and integrin-independent ligands.

Journal Article Biomaterials · December 1999 Endothelial cell adhesion can be enhanced by supplementing integrin-mediated adhesion via fibronectin with the high-affinity avidin-biotin system in which biotin is covalently linked to membrane proteins and avidin binds to biotinylated surfaces (Bhat et a ... Full text Cite

Local monocyte deposition patterns and elevated permeabilities in the rabbit abdominal aorta

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · December 1, 1999 A previous study explored the possible links between the early stages of the atherosclerotic process and the hemodynamic environment in rabbit's aorto-celiac junction. The previous study is expanded by refining the hemodynamic wall parameters through the i ... Cite

Integration of total internal reflection and atomic force microscopy (TIRFM-AFM) to study stress transduction mechanisms in endothelial cells

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 1999 The purpose of this study was to integrate atomic force microscopy (AFM) and total internal reflection fluorescence microscopy (TIRFM) data to determine the effect of localized force application over the cell surface on the cell's focal contacts size and p ... Cite

Effect of fluid viscosity and erythrocytes on monocyte adhesion

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 1999 Monocyte recruitment to vascular endothelium is a key event linked to the development of atherosclerosis. Adhesion is influenced by many parameters including local fluid dynamics, intracellular collisions, and adhesion receptor expression. Elevated medium ... Cite

Relation between non-uniform hemodynamics and sites of altered permeability and lesion growth at the rabbit aorto-celiac junction.

Journal Article Atherosclerosis · March 1999 Using the rabbit's aorto-celiac junction as a representative atherosclerotic model, the hemodynamics of a bifurcating blood vessel are numerically simulated and three hemodynamic parameters are compared. The wall shear stress (WSS), the oscillatory shear i ... Full text Cite

Focal increases in vascular cell adhesion molecule-1 and intimal macrophages at atherosclerosis-susceptible sites in the rabbit aorta after short-term cholesterol feeding.

Journal Article Arteriosclerosis, thrombosis, and vascular biology · February 1999 We tested the hypotheses that vascular cell adhesion molecule-1 (VCAM-1) expression on endothelium at lesion-prone sites in the rabbit aorta correlates with exposure to plasma cholesterol and that macrophage accumulation is associated with endothelial cell ... Full text Cite

Critical factors in basal cell adhesion molecule/lutheran-mediated adhesion to laminin.

Journal Article J Biol Chem · January 8, 1999 Basal cell adhesion molecule (B-CAM) and Lutheran (LU) are two spliceoforms of a single immunoglobulin superfamily protein containing five Ig domains and comprise the sickle (SS) red cell receptor for laminin. We have now analyzed laminin binding to murine ... Full text Link to item Cite

Short-term cell/substrate contact dynamics of subconfluent endothelial cells following exposure to laminar flow.

Journal Article Biotechnology progress · January 1999 The manner in which fluid stresses are transmitted from the apical to the basal surface of the endothelium will influence the dynamics of cell/substrate contacts. Such dynamics could be important in the design of synthetic vascular grafts to promote endoth ... Full text Cite

Integration of total internal reflection microscopy and atomic force microscopy (TIRFM-AFM) to study mechanotransduction in endothelial cells

Journal Article Critical Reviews in Biomedical Engineering · December 1, 1998 Atomic force microscopy (AFM) and total internal reflection fluorescence microscopy (TIRFM) were used simultaneously to determine the effect of localized force application on cell-substrate contact size and position. Fluorescently labeled Human Umbilical V ... Cite

Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells

Journal Article American Journal of Physiology · December 1, 1998 We used a sudden-expansion flow chamber to examine U-937 cell adhesion to unactivated and tumor necrosis factor (TNF)-aactivated human umbilical vein endothelial cells (HUVEC) in recirculating flow. For both unactivated and TNF-a-activated HUVEC, U-937 cel ... Cite

Fibronectin and avidin–biotin as a heterogeneous ligand system for enhanced endothelial cell adhesion

Journal Article Journal of Biomedical Materials Research · September 5, 1998 Full text Cite

Fibronectin and avidin-biotin as a heterogeneous ligand system for enhanced endothelial cell adhesion.

Journal Article Journal of biomedical materials research · September 1998 A preadsorbed layer of "heterogeneous" integrin-dependent and -independent protein was used to enhance initial integrin-mediated endothelial cell attachment and spreading. Glass substrates were treated with fibronectin (Fn) and avidin coupled through adsor ... Full text Cite

Association between secondary flow in models of the aorto-celiac junction and subendothelial macrophages in the normal rabbit.

Journal Article Atherosclerosis · September 1998 In order to examine the association between arterial fluid dynamics and the distribution of subendothelial macrophages in the normal rabbit aorta, steady and pulsatile particle flow visualization was performed in a geometrically realistic model of the rabb ... Full text Cite

Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells.

Journal Article The American journal of physiology · August 1998 We used a sudden-expansion flow chamber to examine U-937 cell adhesion to unactivated and tumor necrosis factor (TNF)-alpha-activated human umbilical vein endothelial cells (HUVEC) in recirculating flow. For both unactivated and TNF-alpha-activated HUVEC, ... Full text Cite

Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY · August 1, 1998 Link to item Cite

Erratum: (The Journal of Clinical Investigation (June 1998) 101:11 (2550-2558))

Journal Article Journal of Clinical Investigation · July 1, 1998 Cite

Engineering the tissue which encapsulates subcutaneous implants. III. Effective tissue response times

Journal Article Journal of Biomedical Materials Research · June 15, 1998 Full text Cite

Engineering the tissue which encapsulates subcutaneous implants. II. Plasma-tissue exchange properties.

Journal Article J Biomed Mater Res · June 15, 1998 This study assesses the plasma-tissue exchange characteristics of the capsular tissue that forms around implants and how they are affected by implant porosity. The number of vessels and their permeability to rhodamine were measured by intravascular injecti ... Full text Link to item Cite

Engineering the tissue which encapsulates subcutaneous implants. III. Effective tissue response times.

Journal Article J Biomed Mater Res · June 15, 1998 The results of two previous studies have shown that implant porosity can be used to increase both the measured diffusion coefficients and the vascularity within the tissue encapsulating long-term subcutaneous implants. This study investigates the hypothesi ... Full text Link to item Cite

Basal cell adhesion molecule/lutheran protein. The receptor critical for sickle cell adhesion to laminin.

Journal Article J Clin Invest · June 1, 1998 Sickle red cells bind significant amounts of soluble laminin, whereas normal red cells do not. Solid phase assays demonstrate that B-CAM/LU binds laminin on intact sickle red cells and that red cell B-CAM/LU binds immobilized laminin, whereas another putat ... Full text Link to item Cite

Using avidin‐mediated binding to enhance initial endothelial cell attachment and spreading

Journal Article Journal of Biomedical Materials Research · April 1998 Full text Cite

Using avidin-mediated binding to enhance initial endothelial cell attachment and spreading.

Journal Article Journal of biomedical materials research · April 1998 Binding between the protein avidin and the vitamin biotin was used as an extrinsic, high affinity receptor-ligand system to augment the intrinsic integrin-dependent cellular adhesion mechanism. Glass substrates were coupled with avidin receptors through an ... Full text Cite

Application of total internal reflection fluorescence microscopy to study cell adhesion to biomaterials.

Journal Article Biomaterials · March 1998 Cell adhesion and function depend upon the formation of adhesive contacts between the cell and substrate. Determination of the cell substrate contact area is necessary in order to understand how biomaterial properties influence cell adhesion. In this revie ... Full text Cite

Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies.

Journal Article J Biomater Sci Polym Ed · 1998 Synthetic vascular grafts do not spontaneously endothelialize in humans and require some form of anticoagulation to maintain patency. Preseeding synthetic graft materials such as expanded polytetrafluoroethylene (ePTFE) and polyethylene terephthalate (PET) ... Full text Link to item Cite

Integration of total internal reflection microscopy and atomic force microscopy (TIRFM-AFM) to study mechanotransduction in endothelial cells

Journal Article Critical Reviews in Biomedical Engineering · 1998 Atomic force microscopy (AFM) and total internal reflection fluorescence microscopy (TIRFM) were used simultaneously to determine the effect of localized force application on cell-substrate contact size and position. Fluorescently labeled Human Umbilical V ... Cite

Engineering the tissue which encapsulates subcutaneous implants. I. Diffusion properties

Journal Article Journal of Biomedical Materials Research · December 5, 1997 Full text Cite

Engineering the tissue which encapsulates subcutaneous implants. I. Diffusion properties.

Journal Article J Biomed Mater Res · December 5, 1997 This report uses normal rat subcutis as a reference point to provide a quantitative analysis of small analyte transport through the tissue which encapsulates implants. Polyvinyl alcohol (PVA) with 60- and 350-micron mean pore size (PVA-60, PVA-350), nonpor ... Full text Link to item Cite

Effect of flow recirculation upon endothelial cell height and shape

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · December 1, 1997 Cite

Spatial distribution of VCAM-1 positive endothelium and intimal macrophages during early hypercholesterolemia

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · December 1, 1997 Cite

The orientation of mammalian skeletal muscle followlng a unidirectional stretch

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · December 1, 1997 Cite

Diffusion properties of tissue which encapsulates subcutaneous implants.

Other ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY · April 13, 1997 Link to item Cite

Avidin-biotin as an extrinsic, high affinity receptor-ligand system to enhance initial cell attachment and spreading.

Journal Article ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY · April 13, 1997 Link to item Cite

Diffusion properties of tissue which encapsulates subcutaneous implants

Other Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering · January 1, 1997 Results are presented of experiments on the diffusion properties of tissue with encapsulates subcutaneous implants. The first experiment was conducted to measure the effective coefficients through capsules which formed around various substrates implanted i ... Cite

Diffusion properties of capsular tissue

Other Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium · December 1, 1996 The effect of capsule formation around a subcutaneous implant is discussed. Polyvinyl alcohol sponge and stainless steel wire mesh materials were implanted. The results show that the diffusion coefficients of fluorescein through capsular tissue are approxi ... Cite

Fibronectin enhances cell spreading and retention on ePTFE under shear in vitro

Other Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium · December 1, 1996 The extent of cell spreading and retention on a modified endothelial cell lining made of expanded polytetrafluoroethylene (ePTFE) under various shear stresses in vitro were examined. The ePTFE was denucleated and treated either by rinsing in sterile phosph ... Cite

Effect of receptor-ligand affinity on the strength of endothelial cell adhesion.

Journal Article Biophysical journal · November 1996 The objective of this study was to determine the effect of receptor-ligand affinity on the strength of endothelial cell adhesion. Linear and cyclic forms of the fibronectin (Fn) cell-binding domain peptide Arg-Gly-Asp (RGD) were covalently immobilized to g ... Full text Cite

A focal stress gradient-dependent mass transfer mechanism for atherogenesis in branching arteries.

Journal Article Medical engineering & physics · June 1996 A new arterial wall permeability function, based on the local wall shear stress gradient, has been developed and employed to simulate enhanced low density lipoprotein transfer across the endothelium. the atherosclerotic model used is that of the aorto-celi ... Full text Cite

THE ZERO-TENSION HYPOTHESIS FOR THE MECHANISM OF ATHEROGENESIS AND THE WALL SHEAR STRESS GRADIENT (WSSG) PREDICTOR EQUATION

Conference ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE) · January 1, 1996 Full text Cite

Effect of fibronectin amount and conformation on the strength of endothelial cell adhesion to HEMA/EMA copolymers.

Journal Article Journal of biomedical materials research · January 1996 The effect of substrate surface hydrophobicity on fibronectin (Fn) adsorption and endothelial cell adhesion strength was studied. Bovine aortic endothelial cells (BAEC) were plated for 2 h with and without preadsorbed Fn on slides coated with homopolymers ... Full text Cite

Numerical investigation and prediction of atherogenic sites in branching arteries.

Journal Article Journal of biomechanical engineering · August 1995 Atherosclerosis, a disease of large- and medium-size arteries, is the chief cause of death in the US and most of the western world. It is widely accepted that the focal nature of the disease in arterial bends, junctions, and bifurcations is directly relate ... Full text Cite

Shear stress induces ATP-independent transient nitric oxide release from vascular endothelial cells, measured directly with a porphyrinic microsensor.

Journal Article Circulation research · August 1995 Shear stress causes the vascular endothelium to release nitric oxide (NO), which is an important regulator of vascular tone. However, direct measurement of NO release after the imposition of laminar flow has not been previously accomplished because of chem ... Full text Cite

The distribution of intimal white blood cells in the normal rabbit aorta.

Journal Article Atherosclerosis · June 1995 Macrophages play an important role in atherogenesis and have been reported within the intima at lesion-prone sites in normocholesterolemic animals as well as infants and children. The objective of this study was to determine the spatial distribution of int ... Full text Cite

Characterization of a sudden expansion flow chamber to study the response of endothelium to flow recirculation.

Journal Article Journal of biomechanical engineering · May 1995 In order to simulate regions of flow separation observed in vivo, a conventional parallel plate flow chamber was modified to produce an asymmetric sudden expansion. The flow field was visualized using light reflecting particles and the size of the recircul ... Full text Cite

Low density lipoprotein transport in the arterial wall of normal and hypercholesterolemic rabbits

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · January 1, 1995 Lesion-prone sites exhibit increased permeability to macromolecules, which may be associated with monocyte adhesion and transmigration. In this study, the association between intimal macrophages and sites of increased 125I-low density lipoprotein (LDL) per ... Cite

Dynamic response of endothelial cells to flow as measured by total internal reflectance fluorescence microscopy

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · January 1, 1995 To study the effect of luminal fluid stress on the abluminal cell surface and its adhesion sites, called focal contacts, total internal fluorescence reflectance microscopy is developed for measuring the dynamic response of endothelial cell focal contacts t ... Cite

Characterization of shear stress dependent release of nitric oxide from endothelial cells in vitro

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · January 1, 1995 Nitric oxide (NO) is a potent vasodilator released by endothelial cells following exposure to flow. Studies of NO release under well defined flow conditions are necessary in order to understand the kinetics of NO release and the regulation of this process. ... Cite

Characterization of sites with elevated LDL permeability at intercostal, celiac, and iliac branches of the normal rabbit aorta.

Journal Article Arteriosclerosis and thrombosis : a journal of vascular biology · February 1994 En face autoradiography of the endothelium was used to quantify the distribution, area, and permeability of sites with enhanced permeability to 125I-low-density lipoprotein (125I-LDL) around the intercostal and celiac arteries and at the iliac bifurcation ... Full text Cite

Quantitative analysis of variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) of cell/substrate contacts.

Journal Article Journal of microscopy · January 1994 Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) allows controlled variation of the illumination depth with the potential of measuring both membrane/substrate separation distances and sizes of focal contacts. VA-TIRFM images are ... Full text Cite

Local conformational changes of vitronectin upon adsorption on glass and silane surfaces

Journal Article Journal of Colloid And Interface Science · January 1, 1994 The conformation of vitronectin (Vn) in solution and adsorbed to hydrophilic and hydrophobic glass was studied using the polarity-sensitive fluor acrylodan. Free sulfhydryl groups of vitronectin were labeled with acrylodan under nondenaturing conditions. T ... Full text Cite

Imaging of cell/substrate contacts on polymers by total internal reflection fluorescence microscopy.

Journal Article Biotechnology progress · January 1994 A simplified model of total internal reflection fluorescence (TIRF) emission of fluorescently labeled cell membranes [Reichert, W. M.; Truskey, G. A. J. Cell Sci. 1990, 96, 219-230] was used to determine the topography of the cell membrane in apposition to ... Full text Cite

A numerical analysis of forces exerted by laminar flow on spreading cells in a parallel plate flow chamber assay.

Journal Article Biotechnology and bioengineering · October 1993 Exposure of spreading anchorage-dependent cells to laminar flow is a common technique to measure the strength of cell adhesion. Since cells protrude into the flow stream, the force exerted by the fluid on the cells is a function of cell shape. To assess th ... Full text Cite

Effect of the conformation and orientation of adsorbed fibronectin on endothelial cell spreading and the strength of adhesion.

Journal Article Journal of biomedical materials research · August 1993 The effect of surface hydrophobicity upon the conformation of the cell binding domain of fibronectin (Fn) and the influence of Fn conformation on bovine aortic endothelial cell (BAEC) adhesion were examined. The free sulfhydryl group of Fn located near the ... Full text Cite

Relationship between 3T3 cell spreading and the strength of adhesion on glass and silane surfaces.

Journal Article Biomaterials · January 1993 Cell detachment by laminar shear stresses was used to characterize cellular interactions with hydrophilic glass and hydrophobic silane. In this study, we examined whether smaller, rounder cells were preferentially detached by laminar flow, and whether cell ... Full text Cite

Altered distribution of mitochondria and actin fibers in 3T3 cells cultured on microcarriers.

Journal Article Biotechnology progress · November 1992 The mitochondria and actin fibers of 3T3 fibroblasts cultured on microcarriers in spinner flasks were visualized using fluorescent stains. In contrast to cells grown on planar surfaces under static or steady laminar flow conditions, cells exposed to higher ... Full text Cite

Total internal reflection fluorescence microscopy (TIRFM). II. Topographical mapping of relative cell/substratum separation distances.

Journal Article Journal of cell science · October 1992 A simplified model of TIRF optics was used to quantitate the relative membrane/substratum separation distances from the spatial pattern of TIRF image brightness. Phase-contrast and total internal reflection fluorescence microscopy (TIRFM) images were colle ... Full text Cite

Measurement of endothelial permeability to 125I-low density lipoproteins in rabbit arteries by use of en face preparations.

Journal Article Circulation research · October 1992 A procedure of en face quantitative autoradiography of the endothelium (Hautchen preparations) was developed to examine regional variations in 125I-low density lipoprotein (125I-LDL) permeability in the arterial wall in vivo. Endothelial preparations from ... Full text Cite

Postadsorption changes in the emission maximum of acrylodan-labeled bovine serum albumin using total internal reflection fluorescence

Journal Article Journal of Colloid And Interface Science · January 1, 1992 Acrylodan (Ac), the thiol reactive analog of the polarity-sensitive fluorescent dye Prodan, was investigated as a site-specific probe for postadsorption conformational changes of bovine serum albumin (BSA) adsorbed to bare and alkyl silane-treated glass co ... Full text Cite

Variable angle total internal reflection fluorescence (TIRF) microscopy of fluorescently labelled lipid films and cells at the glass-liquid interface

Journal Article Annals of Biomedical Engineering · December 1, 1991 Total internal reflection fluorescence (TIRF) microscopy has become the technique of choice for imaging the regions of cells in closest apposition to the substrate surface. For a given wavelength of incident light totally reflected at the glass/solution in ... Cite

Role of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow

Journal Article American Society of Mechanical Engineers, Bioengineering Division (Publication) BED · December 1, 1991 Forces exerted on adherent calls by fluid shear stresses can cause cell detachment. In this study, we examined the effect of cell spreading, actin fiber density, and the conformation of adsorbed adhesion proteins in promoting cell adhesion after a brief ex ... Cite

Regional variations in endothelial permeability to 125I-low density lipoproteins in rabbit arteries

Journal Article Annals of Biomedical Engineering · December 1, 1991 The permeability of the arterial wall to macromolecules is not uniform, and focal regions of clevated permeability may represent the initial stages of atherosclerosis. Hypothesis to explain the focal nature of high permeability regions include distention o ... Cite

Numerical simulation of forces on a spreading cell exposed to flow

Journal Article Annals of Biomedical Engineering · December 1, 1991 When spreading cells are briefly exposed to flow, cell detachment is influenced by both the number of bonds formed and by the shape of the cell. Previous studies indicate a relationship between cell spreading and adhesion. Spreading cells exhibit a variety ... Cite

Quantitation of cell area on glass and fibronectin-coated surfaces by digital image analysis.

Journal Article Biotechnology progress · November 1990 By using digital image processing and analysis, two procedures were developed to rapidly measure the projected area of a field of adherent 3T3 fibroblasts without staining of cell borders. The cell area of newly attached and rounded cells with well-resolve ... Full text Cite

Kinetic studies and unstructured models of lymphocyte metabolism in fed-batch culture.

Journal Article Biotechnology and bioengineering · October 1990 The growth of two lymphocyte cell lines, a hybridoma cell line and a human cutaneous T cell lymphoma (HuT78), was studied in fed-batch culture, and unstructured models of growth developed. A criteria was established to insure that the growth rate varied by ... Full text Cite

The effect of fluid shear stress upon cell adhesion to fibronectin-treated surfaces.

Journal Article Journal of biomedical materials research · October 1990 Cell attachment to and spreading upon a surface is mediated by adhesion molecules, such as fibronectin. The role of fibronectin in maintaining cell adhesion was examined by measuring cell attachment following exposure of cells to laminar flow in a parallel ... Full text Cite

A METHOD TO MEASURE CELL-SEPARATION DISTANCES USING TOTAL INTERNAL-REFLECTION FLUORESCENCE

Journal Article ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY · August 26, 1990 Link to item Cite

Total internal reflection fluorescence (TIRF) microscopy. I. Modelling cell contact region fluorescence.

Journal Article Journal of cell science · June 1990 Total Internal Reflection Fluorescence (TIRF) is a powerful technique for visualizing focal and close contacts between the cell and the surface. Practical application of TIRF has been hampered by the lack of straightforward methods to calculate separation ... Full text Cite

TOTAL INTERNAL-REFLECTION FLUORESCENCE VIDEO MICROSCOPY OF CELL-SURFACE CONTACTS

Conference ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY · September 10, 1989 Link to item Cite

METABOLIC STUDIES OF HYBRIDOMAS IN FED-BATCH BIOREACTOR

Conference ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY · September 25, 1988 Link to item Cite

The effect of membrane filtration upon protein conformation.

Journal Article Journal of parenteral science and technology : a publication of the Parenteral Drug Association · November 1987 Cite

Metabolic cooperation between vascular endothelial cells and smooth muscle cells in co-culture: changes in low density lipoprotein metabolism.

Journal Article The Journal of cell biology · September 1985 A microcarrier co-culture system for aortic endothelial cells and smooth muscle cells (SMCs) was developed as a model for metabolic interactions between cells of the vessel wall. Low density lipoprotein (LDL) metabolism in SMCs was significantly influenced ... Full text Cite

Effects of ammonium ion derived from bovine endothelial cells upon low density lipoprotein degradation in cultured vascular smooth muscle cells.

Journal Article Cell biology international reports · April 1985 Low density lipoprotein (LDL) metabolism in bovine arterial smooth muscle cells (SMC) was increased upon exposure to endothelial cell conditioned medium. The mass of LDL degraded in the SMC lysosomal system was increased, and kinetic analysis demonstrated ... Full text Cite

Kinetic Analysis of Receptor‐Mediated Endocytosis and Lysosomal Degradation in Cultured Cells

Journal Article Annals of the New York Academy of Sciences · January 1, 1984 Full text Cite