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Adam P. Wax

Professor of Biomedical Engineering
Biomedical Engineering
Box 90281, Durham, NC 27708-0281
2571 Ciemas, Durham, NC 27708

Selected Publications


Next-generation endoscopic probe for detection of esophageal dysplasia using combined OCT and angle-resolved low-coherence interferometry.

Journal Article Biomedical optics express · March 2024 Angle-resolved low-coherence interferometry (a/LCI) is an optical technique that enables depth-specific measurements of nuclear morphology, with applications to detecting epithelial cancers in various organs. Previous a/LCI setups have been limited by cost ... Full text Cite

Senior Editor Retirement: Farewell to Ben Dingel, Welcome Szymon Gładysz

Journal Article Optical Engineering · September 1, 2023 Full text Cite

Biophysical Profiling of Sickle Cell Disease Using Holographic Cytometry and Deep Learning.

Journal Article International journal of molecular sciences · July 2023 Sickle cell disease (SCD) is an inherited hematological disorder associated with high mortality rates, particularly in sub-Saharan Africa. SCD arises due to the polymerization of sickle hemoglobin, which reduces flexibility of red blood cells (RBCs), causi ... Full text Cite

Multiscale optical phase fluctuations link disorder strength and fractal dimension of cell structure.

Journal Article Biophysical journal · April 2023 Optical methods for examining cellular structure based on endogenous contrast rely on analysis of refractive index changes to discriminate cell phenotype. These changes can be visualized using techniques such as phase contrast microscopy, detected by light ... Full text Cite

Multiscale Quantitative Phase Imaging Yields Fractal Dimension of Cellular Structures

Conference Novel Techniques in Microscopy, NTM 2023 · January 1, 2023 We demonstrate a novel technique for calculating the fractal dimension of adherent cells using multiscale Quantitative Phase Imaging information. We examine the relationship between this measurement and disorder strength across different cell lines. ... Full text Cite

Dual-Wavelength Holographic Cytometry for Red Blood Cell Disease Diagnosis

Conference Novel Techniques in Microscopy, NTM 2023 · January 1, 2023 Two-wavelength phase microscopy enables quantification of hemoglobin concentration and height of fast-flowing cells in a flow cytometer. This approach shows great potential for point-of-care diagnosis of blood disorders. ... Full text Cite

High throughput QPM for Sickle Cell Disease Detection

Conference Bio-Optics: Design and Application in Proceedings Biophotonics Congress: Optics in the Life Sciences 2023, OMA, NTM, BODA, OMP, BRAIN 2023 · January 1, 2023 We propose to use holographic cytometry to evaluate sickle cell disease patient samples and develop artificial intelligence that can screen for sickling phenotypes. ... Full text Cite

OE Welcomes Our First Patent Review

Journal Article Optical Engineering · December 1, 2022 Full text Cite

Esophageal OCT Imaging Using a Paddle Probe Externally Attached to Endoscope.

Journal Article Digestive diseases and sciences · October 2022 Background and aimsEndoscopic surveillance of Barrett's esophagus (BE) by white light examination is insufficient to diagnose dysplastic change. In this work, we describe an optical imaging method to obtain high-resolution cross-sectional imaging ... Full text Cite

To Zoom or not to Zoom?

Journal Article Optical Engineering · June 1, 2022 Full text Cite

Combined Quantitative Phase Microscopy and Förster Resonance Energy Transfer Imaging for Analyzing Endothelial Cell Shear Stress Response

Conference Optics InfoBase Conference Papers · January 1, 2022 Demonstration of quantitative phase microscopy and Förster resonance energy transfer for analyzing molecular and structural information simultaneously. We focus on signaling pathways and sub-cellular structure for endothelial cells exposed to various flows ... Cite

Analysis of Disorder Strength Across Quantitative Phase Imaging Systems

Conference Optics InfoBase Conference Papers · January 1, 2022 We use three Quantitative Phase Imaging modalities to measure morphological properties of adherent cells. We aim to show that cellular disorder strength differs between systems but can be directly compared by considering system resolution. ... Cite

Enhancing Portable OCT Image Quality via GANs for AI-Based Eye Disease Detection

Conference Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) · January 1, 2022 Optical coherence tomography (OCT) is widely used for detection of ophthalmic diseases, such as glaucoma, age-related macular degeneration (AMD), and diabetic retinopathy. Using a low-coherence-length light source, OCT is able to achieve high axial resolut ... Full text Cite

Deep imaging with 1.3 µm dual-axis optical coherence tomography and an enhanced depth of focus.

Journal Article Biomedical optics express · December 2021 For many clinical applications, such as dermatology, optical coherence tomography (OCT) suffers from limited penetration depth due primarily to the highly scattering nature of biological tissues. Here, we present a novel implementation of dual-axis optical ... Full text Cite

Automated Classification of Breast Cancer Cells Using High-Throughput Holographic Cytometry

Journal Article Frontiers in Physics · November 30, 2021 Holographic cytometry is an ultra-high throughput quantitative phase imaging modality that is capable of extracting subcellular information from millions of cells flowing through parallel microfluidic channels. In this study, we present our findings on the ... Full text Cite

Connectivity-based deep learning approach for segmentation of the epithelium in in vivo human esophageal OCT images.

Journal Article Biomedical optics express · October 2021 Optical coherence tomography (OCT) is used for diagnosis of esophageal diseases such as Barrett's esophagus. Given the large volume of OCT data acquired, automated analysis is needed. Here we propose a bilateral connectivity-based neural network for in ... Full text Cite

Give credit where due: Authorship at OE

Journal Article Optical Engineering · October 1, 2021 Full text Cite

Single Cell Analysis of Stored Red Blood Cells Using Ultra-High Throughput Holographic Cytometry.

Journal Article Cells · September 17, 2021 Holographic cytometry is introduced as an ultra-high throughput implementation of quantitative phase imaging of single cells flowing through parallel microfluidic channels. Here, the approach was applied for characterizing the morphology of individual red ... Full text Link to item Cite

Next-generation endoscopic probe for detection of esophageal dysplasia using combined OCT and angle-resolved low-coherence interferometry.

Journal Article Biomedical optics express · March 2024 Angle-resolved low-coherence interferometry (a/LCI) is an optical technique that enables depth-specific measurements of nuclear morphology, with applications to detecting epithelial cancers in various organs. Previous a/LCI setups have been limited by cost ... Full text Cite

Senior Editor Retirement: Farewell to Ben Dingel, Welcome Szymon Gładysz

Journal Article Optical Engineering · September 1, 2023 Full text Cite

Biophysical Profiling of Sickle Cell Disease Using Holographic Cytometry and Deep Learning.

Journal Article International journal of molecular sciences · July 2023 Sickle cell disease (SCD) is an inherited hematological disorder associated with high mortality rates, particularly in sub-Saharan Africa. SCD arises due to the polymerization of sickle hemoglobin, which reduces flexibility of red blood cells (RBCs), causi ... Full text Cite

Multiscale optical phase fluctuations link disorder strength and fractal dimension of cell structure.

Journal Article Biophysical journal · April 2023 Optical methods for examining cellular structure based on endogenous contrast rely on analysis of refractive index changes to discriminate cell phenotype. These changes can be visualized using techniques such as phase contrast microscopy, detected by light ... Full text Cite

Multiscale Quantitative Phase Imaging Yields Fractal Dimension of Cellular Structures

Conference Novel Techniques in Microscopy, NTM 2023 · January 1, 2023 We demonstrate a novel technique for calculating the fractal dimension of adherent cells using multiscale Quantitative Phase Imaging information. We examine the relationship between this measurement and disorder strength across different cell lines. ... Full text Cite

Dual-Wavelength Holographic Cytometry for Red Blood Cell Disease Diagnosis

Conference Novel Techniques in Microscopy, NTM 2023 · January 1, 2023 Two-wavelength phase microscopy enables quantification of hemoglobin concentration and height of fast-flowing cells in a flow cytometer. This approach shows great potential for point-of-care diagnosis of blood disorders. ... Full text Cite

High throughput QPM for Sickle Cell Disease Detection

Conference Bio-Optics: Design and Application in Proceedings Biophotonics Congress: Optics in the Life Sciences 2023, OMA, NTM, BODA, OMP, BRAIN 2023 · January 1, 2023 We propose to use holographic cytometry to evaluate sickle cell disease patient samples and develop artificial intelligence that can screen for sickling phenotypes. ... Full text Cite

OE Welcomes Our First Patent Review

Journal Article Optical Engineering · December 1, 2022 Full text Cite

Esophageal OCT Imaging Using a Paddle Probe Externally Attached to Endoscope.

Journal Article Digestive diseases and sciences · October 2022 Background and aimsEndoscopic surveillance of Barrett's esophagus (BE) by white light examination is insufficient to diagnose dysplastic change. In this work, we describe an optical imaging method to obtain high-resolution cross-sectional imaging ... Full text Cite

To Zoom or not to Zoom?

Journal Article Optical Engineering · June 1, 2022 Full text Cite

Combined Quantitative Phase Microscopy and Förster Resonance Energy Transfer Imaging for Analyzing Endothelial Cell Shear Stress Response

Conference Optics InfoBase Conference Papers · January 1, 2022 Demonstration of quantitative phase microscopy and Förster resonance energy transfer for analyzing molecular and structural information simultaneously. We focus on signaling pathways and sub-cellular structure for endothelial cells exposed to various flows ... Cite

Analysis of Disorder Strength Across Quantitative Phase Imaging Systems

Conference Optics InfoBase Conference Papers · January 1, 2022 We use three Quantitative Phase Imaging modalities to measure morphological properties of adherent cells. We aim to show that cellular disorder strength differs between systems but can be directly compared by considering system resolution. ... Cite

Enhancing Portable OCT Image Quality via GANs for AI-Based Eye Disease Detection

Conference Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) · January 1, 2022 Optical coherence tomography (OCT) is widely used for detection of ophthalmic diseases, such as glaucoma, age-related macular degeneration (AMD), and diabetic retinopathy. Using a low-coherence-length light source, OCT is able to achieve high axial resolut ... Full text Cite

Deep imaging with 1.3 µm dual-axis optical coherence tomography and an enhanced depth of focus.

Journal Article Biomedical optics express · December 2021 For many clinical applications, such as dermatology, optical coherence tomography (OCT) suffers from limited penetration depth due primarily to the highly scattering nature of biological tissues. Here, we present a novel implementation of dual-axis optical ... Full text Cite

Automated Classification of Breast Cancer Cells Using High-Throughput Holographic Cytometry

Journal Article Frontiers in Physics · November 30, 2021 Holographic cytometry is an ultra-high throughput quantitative phase imaging modality that is capable of extracting subcellular information from millions of cells flowing through parallel microfluidic channels. In this study, we present our findings on the ... Full text Cite

Connectivity-based deep learning approach for segmentation of the epithelium in in vivo human esophageal OCT images.

Journal Article Biomedical optics express · October 2021 Optical coherence tomography (OCT) is used for diagnosis of esophageal diseases such as Barrett's esophagus. Given the large volume of OCT data acquired, automated analysis is needed. Here we propose a bilateral connectivity-based neural network for in ... Full text Cite

Give credit where due: Authorship at OE

Journal Article Optical Engineering · October 1, 2021 Full text Cite

Single Cell Analysis of Stored Red Blood Cells Using Ultra-High Throughput Holographic Cytometry.

Journal Article Cells · September 17, 2021 Holographic cytometry is introduced as an ultra-high throughput implementation of quantitative phase imaging of single cells flowing through parallel microfluidic channels. Here, the approach was applied for characterizing the morphology of individual red ... Full text Link to item Cite

Optical coherence tomography of small intestine allograft biopsies using a handheld surgical probe.

Journal Article J Biomed Opt · September 2021 SIGNIFICANCE: The current gold standard for monitoring small intestinal transplant (IT) rejection is endoscopic visual assessment and biopsy of suspicious lesions; however, these lesions are only superficially visualized by endoscopy. Invasive biopsies pro ... Full text Link to item Cite

Deep learning classification of cervical dysplasia using depth-resolved angular light scattering profiles.

Journal Article Biomedical optics express · August 2021 We present a machine learning method for detecting and staging cervical dysplastic tissue using light scattering data based on a convolutional neural network (CNN) architecture. Depth-resolved angular scattering measurements from two clinical trials were u ... Full text Cite

On the Road Again: Returning to Conferences

Journal Article Optical Engineering · June 1, 2021 Full text Cite

A review of low-cost and portable optical coherence tomography

Journal Article Progress in Biomedical Engineering · 2021 Cite

Combined quantitative phase microscopy and förster resonance energy transfer for analyzing cell ion dynamics

Conference Optics InfoBase Conference Papers · January 1, 2021 We demonstrate the use of quantitative phase microscopy and Förster resonance energy transfer for analyzing molecular and structural information simultaneously. Specifically, we focus on effects of ion channel modulation on signaling pathways and sub-cellu ... Cite

Breast cell cancer progression characterization through holographic cytometry

Conference Optics InfoBase Conference Papers · January 1, 2021 We propose to use holographic cytometry to collect high throughput data on flowing tumorigenic and nontumorigenic cells. We aim to characterize different cell lines using machine learning and morphological parameters analysis. ... Cite

Prospective detection of cervical dysplasia with scanning angle-resolved low coherence interferometry.

Journal Article Biomedical optics express · September 2020 We present a prospective clinical study using angle-resolved low-coherence interferometry (a/LCI) to detect cervical dysplasia via depth resolved nuclear morphology measurements. The study, performed at the Jacobi Medical Center, compares 80 a/LCI optical ... Full text Cite

Spatial scanning of a sample with two-dimensional angle-resolved low-coherence interferometry for analysis of anisotropic scatterers.

Journal Article Biomedical optics express · August 2020 Angle-resolved low-coherence interferometry (a/LCI) measures depth-resolved angular scattering for cell nuclear morphology analysis. 2D a/LCI, developed to collect across two scattering planes, is currently limited by the lack of spatial scanning. Here we ... Full text Cite

Reconstruction of angle-resolved backscattering through a multimode fiber for cell nuclei and particle size determination.

Journal Article APL photonics · July 2020 We demonstrate reconstruction of angle-resolved optical backscattering after transmission through a multimode fiber. Angle-resolved backscattering is an important tool for particle sizing, and has been developed as a diagnostic modality for detecting epith ... Full text Cite

Multimodal Coherent Imaging of Retinal Biomarkers of Alzheimer's Disease in a Mouse Model.

Journal Article Sci Rep · May 13, 2020 We acquired depth-resolved light scattering measurements from the retinas of triple transgenic Alzheimer's Disease (3xTg-AD) mice and wild type (WT) age-matched controls using co-registered angle-resolved low-coherence interferometry (a/LCI) and optical co ... Full text Link to item Cite

Applications of low cost optical coherence tomography

Conference Optics InfoBase Conference Papers · January 1, 2020 We have recently developed a portable, low cost OCT system. This presentation will present an updated design for the optical engine and discuss application in ophthalmology and extensions to fields such as orthopedics, gastroenterology and gynecology where ... Cite

Determination of particle size from reconstructed angular backscattering through a single multimode fiber

Conference Optics InfoBase Conference Papers · January 1, 2020 We demonstrate the use of a transmission matrix approach to reconstruct angular backscattering profiles through a single multimode fiber, with an accurate determination of the scatterer's size based on Mie theory. ... Full text Cite

Clinical use of scanning angle-resolved low coherence interferometry for detection of cervical dysplasia

Conference Optics InfoBase Conference Papers · January 1, 2020 We demonstrate the first clinical implementation of a scanning method to perform angle-resolved low-coherence interferometry (a/LCI) to detect cervical dysplasia over a large area without repositioning. ... Cite

Two-dimensional angle-resolved low-coherence interferometry with spatial scanning

Conference Optics InfoBase Conference Papers · January 1, 2020 We present the integration of spatial scanning into a two-dimensional angle-resolved low-coherence interferometry system. We demonstrate collection of two-dimensional angular scattering over a three-dimensional spatial volume for a variety of samples. ... Cite

Expanded imaging volume for dual-axis optical coherence tomography

Conference Frontiers in Optics - Proceedings Frontiers in Optics + Laser Science APS/DLS · September 8, 2019 We present a method for expanding the scanning volume of a dual-axis optical coherence tomography system (DA-OCT). The modified design increases the lateral scanning area by a factor of 25 and greatly enhances imaging performance in depth using dynamic foc ... Full text Cite

Shear Modulus Measurement by Quantitative Phase Imaging and Correlation with Atomic Force Microscopy.

Journal Article Biophysical journal · August 2019 Many approaches have been developed to characterize cell elasticity. Among these, atomic force microscopy (AFM) combined with modeling has been widely used to characterize cellular compliance. However, such approaches are often limited by the difficulties ... Full text Cite

Light scattering methods for tissue diagnosis.

Journal Article Optica · April 2019 Light scattering has become a common biomedical research tool, enabling diagnostic sensitivity to myriad tissue alterations associated with disease. Light-tissue interactions are particularly attractive for diagnostics due to the variety of contrast mechan ... Full text Open Access Cite

Angular range, sampling and noise considerations for inverse light scattering analysis of nuclear morphology.

Journal Article Journal of biophotonics · February 2019 In recent years, significant work has been devoted to the use of angle-resolved elastic scattering for the extraction of nuclear morphology in tissue. By treating the nucleus as a Mie scattering object, techniques such as angle-resolved low-coherence inter ... Full text Cite

Enhanced depth penetration by dual-axis optical coherence tomography

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2019 Optical imaging techniques generally offer shallow penetration depths due to high scattering in biological tissue. We have recently developed dual-axis optical coherence tomography (DA-OCT) for interferometric imaging at extended depths. By illuminating an ... Full text Cite

First clinical application of low-cost OCT

Journal Article Translational vision science & technology · 2019 Cite

Quantitative phase imaging of erythrocytes under microfluidic constriction in a high refractive index medium reveals water content changes.

Journal Article Microsyst Nanoeng · 2019 Changes in the deformability of red blood cells can reveal a range of pathologies. For example, cells which have been stored for transfusion are known to exhibit progressively impaired deformability. Thus, this aspect of red blood cells has been characteri ... Full text Open Access Link to item Cite

Computational reconstruction of angular scattering distributions through an individual multimode fiber

Conference Optics InfoBase Conference Papers · January 1, 2019 We demonstrate the use of a transmission matrix approach to reconstruct angular scattering profiles measured through an individual multimode fiber, with an eye towards applications in tissue scattering and Mie theory-based analysis. ... Full text Cite

Molecular and biophysical analysis of apoptosis using a combined quantitative phase imaging and fluorescence resonance energy transfer microscope.

Journal Article Journal of biophotonics · December 2018 Apoptotic mechanisms are often dysregulated in cancerous phenotypes. Additionally, many anticancer treatments induce apoptosis and necrosis, and the monitoring of this apoptotic activity can allow researchers to identify therapeutic efficiency. Here, we in ... Full text Cite

Invited Article: Digital refocusing in quantitative phase imaging for flowing red blood cells.

Journal Article APL photonics · November 2018 Quantitative phase imaging (QPI) offers high optical path length sensitivity, probing nanoscale features of live cells, but it is typically limited to imaging just few static cells at a time. To enable utility as a biomedical diagnostic modality, higher th ... Full text Cite

Oct promising as clear point-of-care solution

Journal Article Biophotonics International · September 1, 2018 Cite

Response to Comment on "Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies": A Comment on "How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like?", e201800033.

Journal Article Journal of biophotonics · June 2018 Recently, Maxim A. Yurkin commented on our paper "Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies" as well as on a complementary study "Cell nuclei have lower refract ... Full text Cite

Design and implementation of a low-cost, portable OCT system.

Journal Article Biomedical optics express · March 2018 Optical coherence tomography (OCT) is a widely used biomedical imaging tool, primarily in ophthalmology to diagnose and stage retinal diseases. In order to increase access for a wider range of applications and in low resource settings, we developed a porta ... Full text Cite

Spectroscopic OCT: Towards an effective tool for distinguishing authentic and artificial Chinese freshwater pearls

Journal Article Optical Materials Express · March 1, 2018 Distinguishing authentic and artificial Chinese freshwater pearls involves various tools and techniques, based primarily on visual inspection and spectroscopy. These methods are highly variable and thus not statistically reliable. This study investigates t ... Full text Cite

Cellular shear stiffness reflects progression of arsenic-induced transformation during G1.

Journal Article Carcinogenesis · February 2018 Cancer cells consistently exhibit decreased stiffness; however, the onset and progression of this change have not been characterized. To study the development of cell stiffness changes, we evaluated the shear stiffness of populations of cells during transf ... Full text Cite

Design and implementation of a low-cost, portable OCT system

Conference Optics InfoBase Conference Papers · January 1, 2018 We present a low-cost (under $7,200), portable OCT system to improve ease of access outside of large eye centers. We demonstrate comparable imaging performance to current commercial OCT systems in ex vivo tissue samples. ... Full text Cite

Speckle Reduction in Optical Coherence Tomography at Video Rate

Conference Optical Tomography and Spectroscopy · 2018 Cite

Scanning angle-resolved low coherence interferometry system for clinical detection of cervical dysplasia

Conference Optics InfoBase Conference Papers · January 1, 2018 We present the design of a scanning a/LCI instrument which uses the depth-resolved reflectivity profile to identify the cervical transformation zone and guide a/LCI measurements of nuclear morphology for detection of cervical dysplasia in vivo. ... Full text Cite

Development of a low-cost, portable optical coherence tomography (OCT) system

Conference Optics InfoBase Conference Papers · January 1, 2018 OCT is a widely used biomedical imaging tool, primarily to diagnose and stage retinal diseases. To increase access, we developed a portable, low-cost OCT system with comparable imaging performance to commercially available systems. ... Full text Cite

Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies.

Journal Article Journal of biophotonics · December 2017 The refractive index (RI) of biological materials is a fundamental parameter for the optical characterization of living systems. Numerous light scattering technologies are grounded in a quantitative knowledge of the refractive index at cellular and subcell ... Full text Cite

Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction.

Journal Article Biomedical optics express · December 2017 Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction resolution coherent imaging with quantitative e ... Full text Cite

Scanning system for angle-resolved low-coherence interferometry.

Journal Article Optics letters · November 2017 Angle-resolved low-coherence interferometry (a/LCI) detects precancer by enabling depth-resolved measurements of nuclear morphology in vivo. A significant limitation of a/LCI is the point-probe nature of the method, sampling <0.5  mm2 before pro ... Full text Cite

Roadmap for optofluidics

Journal Article Journal of Optics (United Kingdom) · August 22, 2017 Optofluidics, nominally the research area where optics and fluidics merge, is a relatively new research field and it is only in the last decade that there has been a large increase in the number of optofluidic applications, as well as in the number of rese ... Full text Cite

Dual-axis optical coherence tomography for deep tissue imaging.

Journal Article Opt Lett · June 15, 2017 We have developed dual-axis optical coherence tomography (DA-OCT) which enables deep tissue imaging by using a novel off-axis illumination/detection configuration. DA-OCT offers a 100-fold speed increase compared with its predecessor, multispectral multipl ... Full text Link to item Cite

Refractive index tomography with structured illumination

Journal Article Optica · May 20, 2017 To probe biological questions with significant biophysical, biochemical, and molecular components, an imaging solution compatible with both endogenous and molecular 3D imaging may be necessary. In this work, we show that structured illumination (SI) micros ... Full text Cite

Structured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy.

Journal Article Biomedical optics express · May 2017 Sub-diffraction resolution imaging has played a pivotal role in biological research by visualizing key, but previously unresolvable, sub-cellular structures. Unfortunately, applications of far-field sub-diffraction resolution are currently divided between ... Full text Cite

Feasibility of clinical detection of cervical dysplasia using angle-resolved low coherence interferometry measurements of depth-resolved nuclear morphology.

Journal Article International journal of cancer · March 2017 This study sought to establish the feasibility of using in situ depth-resolved nuclear morphology measurements for detection of cervical dysplasia. Forty enrolled patients received routine cervical colposcopy with angle-resolved low coherence interferometr ... Full text Cite

Label-Free Measurements of Tenofovir Diffusion Coefficients in a Microbicide Gel Using Raman Spectroscopy.

Journal Article Journal of pharmaceutical sciences · February 2017 Confocal Raman spectroscopy was implemented in a new label-free technique to quantify molecular diffusion coefficients within gels. A leading anti-HIV drug, tenofovir, was analyzed in a clinical microbicide gel. The gel was tested undiluted, and in 10%-50% ... Full text Cite

Optical Phase Measurements of Disorder Strength Link Microstructure to Cell Stiffness.

Journal Article Biophysical journal · February 2017 There have been sustained efforts on the part of cell biologists to understand the mechanisms by which cells respond to mechanical stimuli. To this end, many rheological tools have been developed to characterize cellular stiffness. However, measurement of ... Full text Cite

Advances in functional OCT

Journal Article LASER FOCUS WORLD · January 1, 2017 Link to item Cite

Label-free analysis of tenofovir delivery to vaginal tissue using co-registered confocal Raman spectroscopy and optical coherence tomography.

Journal Article PloS one · January 2017 Vaginally applied microbicide products offer a female-controlled strategy for preventing sexual transmission of HIV. Microbicide transport processes are central to their functioning, and there is a clear need for a better understanding of them. To contribu ... Full text Cite

In vivo rat skin flap viability assessment using dual axis spectroscopic optical coherence tomography

Conference Optics InfoBase Conference Papers · January 1, 2017 Dual Axis (DA) spectroscopic optical coherence tomography enables deep tissue imaging and provides diagnostic information. Here it is applied to a McFarlane rat flap model, which exhibits a gradient in tissue injury along the flap length. ... Full text Cite

Nanoscale phase imaging of live cells

Conference Optics InfoBase Conference Papers · January 1, 2017 Quantitative phase imaging uses interferometry to obtain nanoscale information on live cells. Here we describe its use for disease diagnosis and analysis of cell biomechanical properties. ... Full text Cite

Deep imaging of absorption and scattering features by multispectral multiple scattering low coherence interferometry.

Journal Article Biomed Opt Express · October 1, 2016 We have developed frequency domain multispectral multiple scattering low coherence interferometry (ms2/LCI) for deep imaging of absorption and scattering contrast. Using tissue-mimicking phantoms that match the full scattering phase function of human derma ... Full text Link to item Cite

Found in translation: Biophotonics from lab to clinic

Journal Article Optics and Photonics News · September 1, 2016 Full text Cite

Hemoglobin consumption by P. falciparum in individual erythrocytes imaged via quantitative phase spectroscopy.

Journal Article Sci Rep · April 18, 2016 Plasmodium falciparum infection causes structural and biochemical changes in red blood cells (RBCs). To quantify these changes, we apply a novel optical technique, quantitative phase spectroscopy (QPS) to characterize individual red blood cells (RBCs) duri ... Full text Link to item Cite

Toward the assessment of blood oxygenation using multispectral multiple scattering low coherence interferometry

Conference Optics InfoBase Conference Papers · April 18, 2016 Oxygenation of hemolyzed blood was measured with multispectral multiple-scattering low coherence interferometry revealing a high correlation (r2=0.961) with gold-standard spectrophotometer measurements. ... Full text Cite

Guidance of angle-resolved low coherence interferometry using co-located optical coherence tomography on rat esophageal tissue

Conference Optics InfoBase Conference Papers · April 18, 2016 Here we present the analysis of two dimensional angle-resolved low coherence interferometry (2D a/LCI) measurements co-registered with optical coherence tomography (OCT) imaging to guide the a/LCI measurements on an esophageal cancer rat model. ... Full text Cite

Guidance of angle-resolved low coherence interferometry using co-located optical coherence tomography on rat esophageal tissue

Conference Optics InfoBase Conference Papers · April 18, 2016 Here we present the analysis of two dimensional angle-resolved low coherence interferometry (2D a/LCI) measurements co-registered with optical coherence tomography (OCT) imaging to guide the a/LCI measurements on an esophageal cancer rat model. ... Cite

Toward the assessment of blood oxygenation using multispectral multiple scattering low coherence interferometry

Conference Optics InfoBase Conference Papers · April 18, 2016 Oxygenation of hemolyzed blood was measured with multispectral multiple-scattering low coherence interferometry revealing a high correlation (r2=0.961) with gold-standard spectrophotometer measurements. ... Cite

Analyzing spatial correlations in tissue using angle-resolved low coherence interferometry measurements guided by co-located optical coherence tomography.

Journal Article Biomed Opt Express · April 1, 2016 Angle-resolved low coherence interferometry (a/LCI) is an optical technique used to measure nuclear morphology in situ. However, a/LCI is not an imaging modality and can produce ambiguous results when the measurements are not properly oriented to the tissu ... Full text Link to item Cite

Imaging deformation of adherent cells due to shear stress using quantitative phase imaging.

Journal Article Optics letters · January 2016 We present a platform for detecting cellular deformations from mechanical stimuli, such as fluid shear stress, using rapid quantitative phase imaging. Rapid quantitative phase imaging was used to analyze changes in the optical path length of adherent skin ... Full text Cite

Automated Detection of P. falciparum Using Machine Learning Algorithms with Quantitative Phase Images of Unstained Cells.

Journal Article PLoS One · 2016 Malaria detection through microscopic examination of stained blood smears is a diagnostic challenge that heavily relies on the expertise of trained microscopists. This paper presents an automated analysis method for detection and staging of red blood cells ... Full text Open Access Link to item Cite

Corruption of refractive index measurements of spheres using quantitative phase microscopy

Conference Optics InfoBase Conference Papers · January 1, 2016 Recent Quantitative Phase Microscopy experiments have measured the refractive index of a cell nucleus to be lower than the cytoplasm. We suggest a potential error capable of corrupting these measurements. ... Full text Cite

Toward the assessment of blood oxygenation using multispectral multiple scattering low coherence interferometry

Conference Optics InfoBase Conference Papers · January 1, 2016 Oxygenation of hemolyzed blood was measured with multispectral multiple-scattering low coherence interferometry revealing a high correlation (r2=0.961) with gold-standard spectrophotometer measurements. ... Full text Cite

Linking cellular disorder strength and shear stiffness using quantitative phase imaging

Conference Optics InfoBase Conference Papers · January 1, 2016 Quantitative phase imaging (QPI) was implemented to image cells before and during subjugation of shear flow. Simultaneous evaluation of disorder strength and cellular stiffness showed a correlation between the two metrics. ... Full text Cite

Guidance of angle-resolved low coherence interferometry using co-located optical coherence tomography on rat esophageal tissue

Conference Optics InfoBase Conference Papers · January 1, 2016 Here we present the analysis of two dimensional angle-resolved low coherence interferometry (2D a/LCI) measurements co-registered with optical coherence tomography (OCT) imaging to guide the a/LCI measurements on an esophageal cancer rat model. ... Full text Cite

Clinical detection of cervical dysplasia using angle-resolved low coherence interferometry

Conference Optics InfoBase Conference Papers · January 1, 2016 We assess the diagnostic capability of a/LCI in vivo for cervical cancer detection. The nuclear diameter was determined from the a/LCI scans using a Mie theory and a wavelet based hybrid analysis, and the results were compared to histopathology. ... Full text Cite

Evaluation of burn severity in vivo in a mouse model using spectroscopic optical coherence tomography.

Journal Article Biomed Opt Express · September 1, 2015 Clinical management of burn injuries depends upon an accurate assessment of the depth of the wound. Current diagnostic methods rely primarily on subjective visual inspection, which can produce variable results. In this study, spectroscopic optical coherenc ... Full text Link to item Cite

Molecular contrast in interferometric imaging

Conference Conference on Lasers and Electro-Optics Europe - Technical Digest · August 10, 2015 Interferometric imaging offers many advantages for biomedical applications such as optical sectioning/depth resolution. Inclusion of molecular contrast information improves utility but requires additional efforts to either isolate spectroscopic information ... Cite

Quantitative phase imaging with molecular sensitivity using photoacoustic microscopy with a miniature ring transducer.

Journal Article Journal of biomedical optics · August 2015 We present a dual-modality system for both structural and molecular cell imaging based on coregistered quantitative phase imaging (QPI) and photoacoustic microscopy (PAM). The QPI system was based on off-axis holography, whereas the PAM system comprised a ... Full text Cite

Evaluation of hybrid algorithm for analysis of scattered light using ex vivo nuclear morphology measurements of cervical epithelium.

Journal Article Biomed Opt Express · August 1, 2015 We evaluate a new hybrid algorithm for determining nuclear morphology using angle-resolved low coherence interferometry (a/LCI) measurements in ex vivo cervical tissue. The algorithm combines Mie theory based and continuous wavelet transform inverse light ... Full text Link to item Cite

Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue.

Journal Article Biomedical optics express · June 2015 We report the development of a combined confocal Raman spectroscopy (CRS) and optical coherence tomography (OCT) instrument (CRS-OCT) capable of measuring analytes in targeted biological tissues with sub-100-micron spatial resolution. The OCT subsystem was ... Full text Cite

Influence of defocus on quantitative analysis of microscopic objects and individual cells with digital holography.

Journal Article Biomedical optics express · June 2015 Digital holography offers a unique method for studying microscopic objects using quantitative measurements of the optical phase delays of transmitted light. The optical phase may be integrated across the object to produce an optical volume measurement, a p ... Full text Cite

Functional optical coherence tomography: principles and progress.

Journal Article Phys Med Biol · May 21, 2015 In the past decade, several functional extensions of optical coherence tomography (OCT) have emerged, and this review highlights key advances in instrumentation, theoretical analysis, signal processing and clinical application of these extensions. We revie ... Full text Link to item Cite

In vivo burn severity assessment in a mouse model using spectroscopic optical coherence tomography

Conference Bio-Optics: Design and Application, BODA 2015 · April 6, 2015 Depth resolved spectra obtained from spectroscopic optical coherence tomography (SOCT) are used to evaluate burn injuries in vivo in a mouse model. Spectral differences between different burn severities are quantified by a power-law fitting model. ... Full text Cite

Combined raman spectroscopy and optical coherence tomography for measuring analytes in targeted tissues

Conference Optical Molecular Probes, Imaging and Drug Delivery, OMP 2015 · April 6, 2015 We report the development of a combined Raman spectroscopy and optical coherence tomography instrument. The instrument was used to accurately measure physiologically-relevant concentrations of microbicide drugs in ex vivo tissue samples. ... Full text Cite

Detecting mechanically induced displacements in human cell cultures using quantitative phase imaging

Conference Novel Techniques in Microscopy, NTM 2015 · April 6, 2015 We present a platform for detecting cellular deformations from mechanical stimuli. Rapid quantitative phase imaging was used to analyze changes in the optical path length of skin cancer cells during mechanical displacement. ... Full text Cite

Elastic scattering spectroscopy and optical coherence tomography

Chapter · January 1, 2015 Elastically scattered light contains information about the scattering medium with which it has interacted. The elastic scattering process can be interpreted as a change in the momentum of light due to its interaction with a scattering object. By analyzing ... Full text Cite

Noise characterization of supercontinuum sources for low-coherence interferometry applications.

Journal Article Journal of the Optical Society of America. A, Optics, image science, and vision · December 2014 We examine the noise properties of supercontinuum light sources when used in low-coherence interferometry applications. The first application is a multiple-scattering low-coherence interferometry (ms2/LCI) system, where high power and long image acquisitio ... Full text Cite

In vivo analysis of burns in a mouse model using spectroscopic optical coherence tomography.

Journal Article Opt Lett · October 1, 2014 Spectroscopic analysis of biological tissues can provide insight into changes in structure and function due to disease or injury. Depth-resolved spectroscopic measurements can be implemented for tissue imaging using optical coherence tomography (OCT). Here ... Full text Link to item Cite

Wavelet transform fast inverse light scattering analysis for size determination of spherical scatterers.

Journal Article Biomedical optics express · October 2014 We present a fast approach for size determination of spherical scatterers using the continuous wavelet transform of the angular light scattering profile to address the computational limitations of previously developed sizing techniques. The potential accur ... Full text Cite

Fast wide-field photothermal and quantitative phase cell imaging with optical lock-in detection.

Journal Article Biomedical optics express · August 2014 We present a fast, wide-field holography system for detecting photothermally excited gold nanospheres with combined quantitative phase imaging. An interferometric photothermal optical lock-in approach (POLI) is shown to improve SNR for detecting nanopartic ... Full text Cite

Quantitative phase microscopy with off-axis optical coherence tomography.

Journal Article Optics letters · April 2014 We have developed a modality for quantitative phase imaging within spectral domain optical coherence tomography based on using an off-axis reference beam. By tilting the propagation of the reference beam relative to that of the sample beam, a spatially var ... Full text Cite

Analysis of vaginal microbicide film hydration kinetics by quantitative imaging refractometry.

Journal Article PloS one · January 2014 We have developed a quantitative imaging refractometry technique, based on holographic phase microscopy, as a tool for investigating microscopic structural changes in water-soluble polymeric materials. Here we apply the approach to analyze the structural d ... Full text Cite

Sensitivity of coded aperture Raman spectroscopy to analytes beneath turbid biological tissue and tissue-simulating phantoms.

Journal Article Journal of biomedical optics · January 2014 Traditional slit-based spectrometers have an inherent trade-off between spectral resolution and throughput that can limit their performance when measuring diffuse sources such as light returned from highly scattering biological tissue. Recently, multieleme ... Full text Cite

Deep tissue imaging using spectroscopic analysis of multiply scattered light

Journal Article Optica · January 1, 2014 Scattering limits the penetration depth of most optical imaging techniques. Efforts to overcome this limitation often require complex optical or computational schemes. We have developed a new method of assessing tissue properties based on spectroscopic ana ... Full text Cite

Preface

Chapter · January 1, 2014 Full text Cite

Preface

Journal Article Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) · January 1, 2014 Full text Cite

Fourier domain multispectral multiple scattering low coherence interferometry.

Journal Article Applied optics · December 2013 We have implemented multispectral multiple scattering low coherence interferometry (ms2/LCI) with Fourier domain data collection. The ms2/LCI system is designed to localize features with spectroscopic contrast with millimeter resolution up to 1 cm deep in ... Full text Cite

A calibration standard for two-dimensional angle-resolved low-coherence interferometry

Journal Article Optical Trapping Applications, OTA 2013 · November 21, 2013 We have developed phantoms for use with two-dimensional angle-resolved lowcoherence interferometry. Here we demonstrate standardized multi-scale structural information for light scattering measurements. © 2013 The Optical Society. ... Cite

A calibration standard for two-dimensional angle-resolved low-coherence interferometry

Journal Article Bio-Optics: Design and Application, BODA 2013 · November 18, 2013 We have developed phantoms for use with two-dimensional angle-resolved lowcoherence interferometry. Here we demonstrate standardized multi-scale structural information for light scattering measurements. Optics in the Life Sciences Congress Technical Digest ... Cite

Multimodal multiplex raman spectroscopy and optical coherence tomography of tissue for drug monitoring

Journal Article Optical Molecular Probes, Imaging and Drug Delivery, OMP 2013 · January 1, 2013 We demonstrate efficient and high resolution Raman spectroscopy in tissue through a fiber bundle and the use of a custom multimodal multiplex spectrometer. Combined Raman and OCT measurements allow for drug monitoring in tissue explants. ©2013 The Optical ... Full text Cite

Measuring dilution of microbicide gels with optical imaging.

Journal Article PloS one · January 2013 We present a novel approach for measuring topical microbicide gel dilution using optical imaging. The approach compares gel thickness measurements from fluorimetry and multiplexed low coherence interferometry in order to calculate dilution of a gel. As a m ... Full text Cite

Feasibility study of brain tumor delineation using immunolabeled gold nanorods.

Journal Article Biomed Opt Express · 2013 Effective treatment of patients with malignant brain tumors requires surgical resection of a high percentage of the bulk tumor. Surgeons require a method that enables delineation of tumor margins, which are not visually distinct by eye. In this study, the ... Full text Link to item Cite

Feasibility of brain tumor delineation using immunolabeled gold nanorods

Journal Article Optical Molecular Probes, Imaging and Drug Delivery, OMP 2013 · January 1, 2013 The enhanced scattering of gold nanorods in the near-infrared regime provides excellent contrast for imaging within biological tissues. This study demonstrates the capability of anti-EGFR nanorods to distinguish xenograft glioblastomas from healthy murine ... Full text Cite

Plasmonic nanoparticles as molecular contrast agents in interferometric imaging

Journal Article Optical Molecular Probes, Imaging and Drug Delivery, OMP 2013 · January 1, 2013 Plasmonic nanoparticles offer unique optical characteristics, including strong spectrally distinct signatures which make them useful as contrast agents. Here various applications are presented and the benefits of nanoparticles are discussed. ©2013 The Opti ... Full text Cite

A calibration standard for two-dimensional angle-resolved low-coherence interferometry

Journal Article Optical Molecular Probes, Imaging and Drug Delivery, OMP 2013 · January 1, 2013 We have developed phantoms for use with two-dimensional angle-resolved lowcoherence interferometry. Here we demonstrate standardized multi-scale structural information for light scattering measurements. ©2013 The Optical Society (OSA). ... Full text Cite

A scattering phantom for observing long range order with two-dimensional angle-resolved Low-Coherence Interferometry.

Journal Article Biomedical optics express · January 2013 Angle-resolved low coherence interferometry (a/LCI) is an approach for assessing tissue structure based on light scattering data. Recent advances in a/LCI have extended the analysis to study scattering distributions in two dimensions. In order to provide s ... Full text Cite

Nuclear morphology measurements with angle-resolved low coherence interferometry for application to cell biology and early cancer detection.

Journal Article Studies in health technology and informatics · January 2013 The study of intact, living cells using non-invasive optical spectroscopic methods offers the opportunity to assess cellular structure and organization in a way that is not possible with commonly used cell biology imaging techniques. We have developed a no ... Cite

Wigner distribution: Friend to biophotonics

Journal Article Optics InfoBase Conference Papers · January 1, 2013 The Wigner distribution provides joint information of a signal in two conjugate domains. Unique insights can be obtained into biophotonics problems by using this formalism to examine the coherence of optical fields. © 2013 Optical Society of America. ... Cite

Molecular imaging true color spectroscopic (METRiCS) optical coherence tomography

Chapter · January 1, 2013 Optical coherence tomography (OCT) is a cross-sectional high-resolution optical imaging modality that operates under the principles of low coherence interferometry (LCI) to enable optical sectioning, or depth resolution, within a biological sample [1]. OCT ... Full text Cite

Wigner distribution: Friend to biophotonics

Conference Optics InfoBase Conference Papers · January 1, 2013 The Wigner distribution provides joint information of a signal in two conjugate domains. Unique insights can be obtained into biophotonics problems by using this formalism to examine the coherence of optical fields. © 2013 Optical Society of America. ... Full text Cite

Three-dimensional molecular imaging with photothermal optical coherence tomography.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2013 Optical coherence tomography (OCT) is a three-dimensional optical imaging technique that has been successfully implemented in ophthalmology for imaging the human retina, and in studying animal models of disease. OCT can nondestructively visualize structura ... Full text Cite

Comparative review of interferometric detection of plasmonic nanoparticles.

Journal Article Biomedical optics express · January 2013 Noble metal nanoparticles exhibit enhanced scattering and absorption at specific wavelengths due to a localized surface plamson resonance. This unique property can be exploited to enable the use of plasmonic nanoparticles as contrast agents in optical imag ... Full text Cite

Coherence imaging for early cancer detection

Journal Article 2012 Conference on Lasers and Electro-Optics, CLEO 2012 · December 6, 2012 We have developed multiple spectroscopic coherence imaging methods to assess pre-cancerous states by isolating scattering signatures from specific tissue locations. We describe the basis, validation and development of the approaches and recent clinical res ... Cite

Spectrally multiplexed photothermal OCT and novel detection methods

Journal Article 2012 Conference on Lasers and Electro-Optics, CLEO 2012 · December 6, 2012 We demonstrate simultaneous detection of two nanoparticle species using phase-sensitive optical coherence tomography with photothermal excitation at two wavelengths matched to the distinct absorption peaks of the nanoparticles. To minimize bulk heating of ... Cite

Spectrally multiplexed photothermal OCT and novel detection methods

Journal Article CLEO: Applications and Technology, CLEO_AT 2012 · December 1, 2012 We demonstrate simultaneous detection of two nanoparticle species using phasesensitive optical coherence tomography with photothermal excitation at two wavelengths matched to the distinct absorption peaks of the nanoparticles. To minimize bulk heating of t ... Cite

Spectrally multiplexed photothermal OCT and novel detection methods

Journal Article Optics InfoBase Conference Papers · December 1, 2012 We demonstrate simultaneous detection of two nanoparticle species using phasesensitive optical coherence tomography with photothermal excitation at two wavelengths matched to the distinct absorption peaks of the nanoparticles. To minimize bulk heating of t ... Cite

In vivo optical imaging of human vaginal gel thickness distributions with a probe-based, dual-modality instrument.

Journal Article Journal of biomedical optics · November 2012 ABSTRACT. We used a probe-based dual-modality optical imaging instrument to measure in vivo coating thickness distributions of a gel distributed along the vaginal lumen, in a clinical study. The gel was a surrogate for one delivering an anti-HIV topical mi ... Full text Cite

Phase-sensitive OCT imaging of multiple nanoparticle species using spectrally multiplexed single pulse photothermal excitation.

Journal Article Biomedical optics express · October 2012 We apply phase-sensitive optical coherence tomography to image multiple nanoparticle species with two excitation wavelengths matched to their distinct absorption peaks. Using different modulation frequencies, multiple species collocated within the sample c ... Full text Cite

Multispectral nanoparticle contrast agents for true-color spectroscopic optical coherence tomography.

Journal Article Biomedical optics express · August 2012 We have recently developed a novel dual window scheme for processing spectroscopic OCT images to provide spatially resolved true color imaging of chromophores in scattering samples. Here we apply this method to measure the extinction spectra of plasmonic n ... Full text Cite

Imaging contrast and resolution in multiply scattered low coherence interferometry

Journal Article IEEE Journal on Selected Topics in Quantum Electronics · June 20, 2012 With recent results demonstrating imaging using low coherence interferometry to detect multiply scattered light, signal attenuation and imaging resolution remains to be an open question. To address this, we present detailed modeling results of light propag ... Full text Cite

Quantitative phase spectroscopy.

Journal Article Biomedical optics express · May 2012 Quantitative phase spectroscopy is presented as a novel method of measuring the wavelength-dependent refractive index of microscopic volumes. Light from a broadband source is filtered to an ~5 nm bandwidth and rapidly tuned across the visible spectrum in 1 ... Full text Cite

Dynamic quantitative microscopy and nanoscopy of red blood cells in sickle cell disease

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 16, 2012 We have applied wide-field digital interferometric techniques to quantitatively image sickle red blood cells (RBCs) [1] in a noncontact label-free manner, and measure the nanometer-scale fluctuations in their thickness as an indication of their stiffness. ... Full text Cite

Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis.

Journal Article Methods (San Diego, Calif.) · February 2012 Noble metal nanoparticles hold great potential as optical contrast agents due to a unique feature, known as the plasmon resonance, which produces enhanced scattering and absorption at specific frequencies. The plasmon resonance also provides a spectral tun ... Full text Cite

Angle-resolved low-coherence interferometry: an optical biopsy technique for clinical detection of dysplasia in Barrett's esophagus.

Journal Article Expert review of gastroenterology & hepatology · February 2012 Angle-resolved low-coherence interferometry (a/LCI) is an optical biopsy technique that measures scattered light from tissue to determine nuclear size with submicron-level accuracy. The a/LCI probe can be deployed through the accessory channel of a standar ... Full text Cite

Assessing nanoparticle concentration using METRiCS optical coherence tomography

Journal Article Biomedical Optics, BIOMED 2012 · January 1, 2012 We have recently developed METRiCS OCT to provide spatially resolved molecular information of chromosomes. Here we apply this method to measure the extinction spectra of nanoparticles at various concentrations for potential in vivo applications. © 2010 Opt ... Full text Cite

Spectral-domain differential interference contrast (SD-DIC) microscopy for measuring live cell dynamics

Journal Article Biomedical Optics, BIOMED 2012 · January 1, 2012 We present live cell imaging using spectral-domain differential interference contrast microscopy, a novel technique for high-resolution, quantitative measurement of optical pathlength gradients. Imaging and dynamic monitoring of live neurons and cardiomyoc ... Full text Cite

Spectrally multiplexed photothermal OCT and novel detection methods

Journal Article CLEO: Applications and Technology, CLEO_AT 2012 · January 1, 2012 We demonstrate simultaneous detection of two nanoparticle species using phasesensitive optical coherence tomography with photothermal excitation at two wavelengths matched to the distinct absorption peaks of the nanoparticles. To minimize bulk heating of t ... Full text Cite

Novel optical signature for sickle cell trait red blood cells

Journal Article Frontiers in Optics, FIO 2012 · January 1, 2012 We identified unique optical signatures for sickle cell trait, a condition where heterozygous individuals are carriers for the hemoglobin allele that causes sickle cell anemia, by using wide-field interferometric microscopy. © OSA 2012. ... Full text Cite

Coherence imaging for early cancer detection

Journal Article CLEO: Applications and Technology, CLEO_AT 2012 · January 1, 2012 We have developed multiple spectroscopic coherence imaging methods to assess pre-cancerous states by isolating scattering signatures from specific tissue locations. We describe the basis, validation and development of the approaches and recent clinical res ... Full text Cite

High-efficiency diffuse Raman spectroscopy through a fiber bundle.

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · January 2012 Conventional spectrometers are limited in the amount of light they accept because of the requirement for narrow input apertures. A trade-off must generally be made between spectral resolution and input aperture width. This is especially a problem for perfo ... Full text Cite

Optical spectroscopy of biological cells

Journal Article Advances in Optics and Photonics · January 1, 2012 Optical spectroscopy has seen expanding use for the study of biological cells in recent years. An overview of relevant spectroscopic techniques is presented, and applications to biological cells are reviewed. © 2010 Optical Society of America. ... Full text Cite

Controlled synthesis of gold nanorods and application to brain tumor delineation

Conference Optics InfoBase Conference Papers · December 1, 2011 Gold nanorods are candidates for tumor delineation due to their unique optical properties and biocompatibility. The optical characteristics of nanorods are tuned during synthesis. Anti-EGFR nanorods effectively label tumors within brain slices. © 2011 OSA. ... Cite

Time-resolved imaging refractometry of microbicidal films using quantitative phase microscopy.

Journal Article Journal of biomedical optics · December 2011 Quantitative phase microscopy is applied to image temporal changes in the refractive index (RI) distributions of solutions created by microbicidal films undergoing hydration. We present a novel method of using an engineered polydimethylsiloxane structure a ... Full text Cite

Molecular imaging true-colour spectroscopic optical coherence tomography.

Journal Article Nat Photonics · December 1, 2011 Molecular imaging holds a pivotal role in medicine due to its ability to provide invaluable insight into disease mechanisms at molecular and cellular levels. To this end, various techniques have been developed for molecular imaging, each with its own advan ... Full text Link to item Cite

Nonlinear phase dispersion spectroscopy.

Journal Article Optics letters · December 2011 Nonlinear phase dispersion spectroscopy is introduced as a means to retrieve wideband, high spectral resolution profiles of the wavelength-dependent real part of the refractive index. The method is based on detecting dispersion effects imparted to a light ... Full text Cite

Quantitative phase microscopy of biological cell dynamics by wide-field digital interferometry

Journal Article Springer Series in Surface Sciences · December 1, 2011 Interferometric phase measurements of wide-field images of biological cells provide a quantitative tool for cell biology, as well as for medical diagnosis and monitoring. Visualizing rapid dynamic cell phenomena by interferometric phase microscopy can be p ... Full text Cite

Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles.

Journal Article Journal of biomedical optics · November 2011 This work presents simultaneous imaging and detection of three different cell receptors using three types of plasmonic nanoparticles (NPs). The size, shape, and composition-dependent scattering profiles of these NPs allow for a system of multiple distinct ... Full text Cite

Monitoring of receptor dimerization using plasmonic coupling of gold nanoparticles.

Journal Article ACS nano · November 2011 The dimerization of receptors on the cell membrane is an important step in the activation of cell signaling pathways. Several methods exist for observing receptor dimerization, including coimmunoprecipitation, chemical cross-linking, and fluorescence reson ... Full text Cite

Detection of intestinal dysplasia using angle-resolved low coherence interferometry.

Journal Article J Biomed Opt · October 2011 Angle-resolved low coherence interferometry (a/LCI) is an optical biopsy technique that allows for depth-resolved, label-free measurement of the average size and optical density of cell nuclei in epithelial tissue to assess the tissue health. a/LCI has pre ... Full text Link to item Cite

Design and validation of a multiplexed low coherence interferometry instrument for in vivo clinical measurement of microbicide gel thickness distribution.

Journal Article Biomedical optics express · October 2011 We present a multiplexed, Fourier-domain low coherence interferometry (mLCI) instrument for in vivo measurement of intravaginal microbicide gel coating thickness distribution over the surface of the vaginal epithelium. The mLCI instrument uses multiple del ... Full text Cite

Stem cell differentiation indicated by noninvasive photonic characterization and fractal analysis of subcellular architecture.

Journal Article Integrative biology : quantitative biosciences from nano to macro · August 2011 We hypothesised that global structural changes in stem cells would manifest with differentiation, and that these changes would be observable with light scattering microscopy. Analysed with a fractal dimension formalism, we observed significant structural c ... Full text Cite

Coherent detection of diffusely scattered light for extending depth using multiply scattered low coherence interferometry

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · May 12, 2011 Interferometric based imaging has a number of advantages over direct detection of photons, including very high time resolution, shot noise limited sensitivity, and reasonable cost. In spite of these advantages, diffuse optical techniques have almost exclus ... Full text Cite

Simultaneous confocal fluorescence microscopy and optical coherence tomography for drug distribution and tissue integrity assessment

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · May 2, 2011 The effectiveness of microbicidal gels, topical products developed to prevent infection by sexually transmitted diseases including HIV/AIDS, is governed by extent of gel coverage, pharmacokinetics of active pharmaceutical ingredients (APIs), and integrity ... Full text Cite

Whole cell imaging based on wide-field interferometric phase microscopy and its application to cardiomyocytes

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 29, 2011 Whole cell imaging is a novel technique using which the time-dependent quantitative phase profiles of live unstained biological cells are analyzed numerically to learn on the cell functionally. Dynamic phase profiles of the sample are first acquired by wid ... Full text Cite

Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 29, 2011 This work presents simultaneous imaging and detection of three types of cell receptors using three types of plasmonic nanoparticles. The size, shape, and composition-dependent scattering profiles of these particles allow for a system of multiple distinct m ... Full text Cite

Determining size, shape, and orientation of non-spherical scatterers using the fiber optic interferometric two-dimensional scattering (FITS) system

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · March 31, 2011 Angle-resolved scattering measurements have shown promise as a method of detecting neoplasia and analyzing cellular structure. Recently we have developed new systems for interferometric measurement of two-dimensional, depth resolved scattered fields with e ... Full text Cite

Fourier domain low coherence interferometry for detection of early colorectal cancer development in the AOM rat model

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · March 31, 2011 We present Fourier domain low coherence interferometry (fLCI) applied to the detection of preneoplastic changes in the colon using the ex-vivo azoxymethane (AOM) rat carcinogenesis model. fLCI measures depth resolved spectral oscillations, also known as lo ... Full text Cite

Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry.

Journal Article J Biomed Opt · March 2011 We have applied wide-field digital interferometry (WFDI) to examine the morphology and dynamics of live red blood cells (RBCs) from individuals who suffer from sickle cell anemia (SCA), a genetic disorder that affects the structure and mechanical propertie ... Full text Link to item Cite

Polarization mapping of nanoparticle plasmonic coupling.

Journal Article Optics letters · March 2011 We propose the use of polarization mapping as a tool to better separate the effects of plasmonic coupling from the local refractive index for molecular imaging and biosensing using gold nanoparticles. Polarization mapping allows identification of the ortho ... Full text Cite

Spectral-domain differential interference contrast microscopy.

Journal Article Optics letters · February 2011 We present a fiber-optic low-coherence imaging technique, termed spectral-domain differential interference contrast microscopy (SD-DIC), for quantitative DIC imaging of both reflective surfaces and transparent biological specimens. SD-DIC combines the comm ... Full text Cite

Imaging beyond the ballistic limit in coherence imaging using multiply scattered light.

Journal Article Optics express · February 2011 We present an imaging system based on low coherence interferometric detection of multiply scattered light for extended depth imaging into highly scattering media. By incorporating angle-resolved detection, coherence imaging with multiply scattered photons ... Full text Cite

Photothermal optical coherence tomography for molecular imaging

Journal Article Optics InfoBase Conference Papers · 2011 Cite

Spectral-domain differential interference contrast microscopy

Journal Article Optics InfoBase Conference Papers · 2011 Cite

Photothermal optical coherence tomography for molecular imaging

Journal Article Optics InfoBase Conference Papers · 2011 Molecular imaging using Photothermal Optical Coherence Tomography (OCT) was demonstrated with antibody-conjugated gold nanoparticles in phantoms and three-dimensional tissue constructs. Cells over-expressing the epidermal growth factor receptor (EGFR) were ... Cite

Whole-cell analysis of cardiomyocytes with combined quantitative phase and two-channel fluorescence microscopy

Journal Article Optics InfoBase Conference Papers · 2011 We have developed a novel microscope combining quantitative phase and fluorescence microscopy to perform quantitative analysis of dynamic cardiomyocyte contraction. Phase-based parameters are informed by molecular specificity of fluorescence images. © 2011 ... Cite

Real-time quantitative phase and dual-channel fluorescence microscopy for studying cellular and biomolecular dynamics

Journal Article Optics InfoBase Conference Papers · 2011 We have developed a microscope that simultaneously captures quantitative phase measurements and two distinct fluorescence images on a single camera. This microscope is an effective tool for investigating cellular dynamics with molecular specificity. © 2011 ... Cite

Spectral-domain differential interference contrast microscopy

Journal Article Optics InfoBase Conference Papers · 2011 We present a novel imaging technique, termed spectral-domain DIC microscopy, for high-resolution quantitative measurement of optical pathlength gradients. Imaging of resolution target and live cardiomyocytes were demonstrated with 36pm resolution. © 2011 O ... Cite

Multimodal optical detection of intravaginal microbicide gel coating thickness distribution

Journal Article Optics InfoBase Conference Papers · January 1, 2011 A clinical optical imaging probe incorporating simultaneous fluorescence and low coherence interferometry (LCI) imaging optics was developed. A clinical study was performed to compare LCI and fluorimetry in measuring intravaginal microbicide gel coating th ... Cite

Spectroscopic optical coherence tomography for quantitative molecular imaging

Journal Article Optics InfoBase Conference Papers · January 1, 2011 Advances in spectroscopic OCT have allowed for quantitative analysis of endogenous contrast agents. Here, we will use SOCT to achieve quantitative molecular imaging using various exogenous contrast agents spanning the visible region of the spectrum. © 2011 ... Cite

Multimodal optical detection of intravaginal microbicide gel coating thickness distribution

Journal Article Optics InfoBase Conference Papers · January 1, 2011 A clinical optical imaging probe incorporating simultaneous fluorescence and low coherence interferometry (LCI) imaging optics was developed. A clinical study was performed to compare LCI and fluorimetry in measuring intravaginal microbicide gel coating th ... Full text Cite

Spectroscopic optical coherence tomography for quantitative molecular imaging

Journal Article Optics InfoBase Conference Papers · January 1, 2011 Advances in spectroscopic OCT have allowed for quantitative analysis of endogenous contrast agents. Here, we will use SOCT to achieve quantitative molecular imaging using various exogenous contrast agents spanning the visible region of the spectrum. © 2011 ... Full text Cite

Quantitative analysis of three-dimensional biological cells using interferometric microscopy

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2011 Live biological cells are three-dimensional microscopic objects that constantly adjust their sizes, shapes and other biophysical features. Wide-field digital interferometry (WFDI) is a holographic technique that is able to record the complex wavefront of t ... Full text Cite

Photothermal optical coherence tomography for molecular imaging

Conference Optics InfoBase Conference Papers · January 1, 2011 Molecular imaging using Photothermal Optical Coherence Tomography (OCT) was demonstrated with antibody-conjugated gold nanoparticles in phantoms and three-dimensional tissue constructs. Cells over-expressing the epidermal growth factor receptor (EGFR) were ... Cite

Real-time quantitative phase and dual-channel fluorescence microscopy for studying cellular and biomolecular dynamics

Conference Optics InfoBase Conference Papers · January 1, 2011 We have developed a microscope that simultaneously captures quantitative phase measurements and two distinct fluorescence images on a single camera. This microscope is an effective tool for investigating cellular dynamics with molecular specificity. © 2011 ... Cite

Whole-cell analysis of cardiomyocytes with combined quantitative phase and two-channel fluorescence microscopy

Conference Optics InfoBase Conference Papers · January 1, 2011 We have developed a novel microscope combining quantitative phase and fluorescence microscopy to perform quantitative analysis of dynamic cardiomyocyte contraction. Phase-based parameters are informed by molecular specificity of fluorescence images. © 2011 ... Cite

Spectral-domain differential interference contrast microscopy

Conference Optics InfoBase Conference Papers · January 1, 2011 We present a novel imaging technique, termed spectral-domain DIC microscopy, for high-resolution quantitative measurement of optical pathlength gradients. Imaging of resolution target and live cardiomyocytes were demonstrated with 36pm resolution. © 2011 O ... Cite

Multimodal optical detection of intravaginal microbicide gel coating thickness distribution

Journal Article Optics InfoBase Conference Papers · 2011 A clinical optical imaging probe incorporating simultaneous fluorescence and low coherence interferometry (LCI) imaging optics was developed. A clinical study was performed to compare LCI and fluorimetry in measuring intravaginal microbicide gel coating th ... Cite

Spectroscopic optical coherence tomography for quantitative molecular imaging

Journal Article Optics InfoBase Conference Papers · 2011 Advances in spectroscopic OCT have allowed for quantitative analysis of endogenous contrast agents. Here, we will use SOCT to achieve quantitative molecular imaging using various exogenous contrast agents spanning the visible region of the spectrum. © 2011 ... Cite

Spectral-domain differential interference contrast microscopy

Conference Optics InfoBase Conference Papers · January 1, 2011 We present a novel imaging technique, termed spectral-domain DIC microscopy, for high-resolution quantitative measurement of optical pathlength gradients. Imaging of resolution target and live cardiomyocytes were demonstrated with 36pm resolution. © 2011 O ... Full text Cite

Real-time quantitative phase and dual-channel fluorescence microscopy for studying cellular and biomolecular dynamics

Conference Optics InfoBase Conference Papers · January 1, 2011 We have developed a microscope that simultaneously captures quantitative phase measurements and two distinct fluorescence images on a single camera. This microscope is an effective tool for investigating cellular dynamics with molecular specificity. © 2011 ... Full text Cite

Whole-cell analysis of cardiomyocytes with combined quantitative phase and two-channel fluorescence microscopy

Conference Optics InfoBase Conference Papers · January 1, 2011 We have developed a novel microscope combining quantitative phase and fluorescence microscopy to perform quantitative analysis of dynamic cardiomyocyte contraction. Phase-based parameters are informed by molecular specificity of fluorescence images. © 2011 ... Full text Cite

Controlled synthesis of gold nanorods and application to brain tumor delineation

Conference Optics InfoBase Conference Papers · January 1, 2011 Gold nanorods are candidates for tumor delineation due to their unique optical properties and biocompatibility. The optical characteristics of nanorods are tuned during synthesis. Anti-EGFR nanorods effectively label tumors within brain slices. © 2011 OSA. ... Full text Cite

Photothermal optical coherence tomography for molecular imaging

Conference Optics InfoBase Conference Papers · January 1, 2011 Molecular imaging using Photothermal Optical Coherence Tomography (OCT) was demonstrated with antibody-conjugated gold nanoparticles in phantoms and three-dimensional tissue constructs. Cells over-expressing the epidermal growth factor receptor (EGFR) were ... Full text Cite

Plasmonic flow cytometry by immunolabeled nanorods.

Journal Article Cytometry A · January 2011 Fluorescence-based flow cytometry measures multiple cellular characteristics, including levels of receptor expression, by assessing the fluorescence intensity from a population of cells whose cell surface receptors are bound by a fluorescently labeled anti ... Full text Link to item Cite

Nuclear morphology measurements with angle-resolved low coherence interferometry for application to cell biology and early cancer detection.

Journal Article Analytical cellular pathology (Amsterdam) · January 2011 The study of intact, living cells using non-invasive optical spectroscopic methods offers the opportunity to assess cellular structure and organization in a way that is not possible with commonly used cell biology imaging techniques. We have developed a no ... Full text Cite

Design and validation of an angle-resolved low-coherence interferometry fiber probe for in vivo clinical measurements of depth-resolved nuclear morphology.

Journal Article Journal of biomedical optics · January 2011 We present a novel Fourier-domain angle-resolved low-coherence interferometry (a /LCI) fiber probe designed for in vivo clinical application in gastrointestinal endoscopy. The a/LCI technique measures the depth-resolved angular scattering distribution to d ... Full text Cite

Detection of dysplasia in Barrett's esophagus with in vivo depth-resolved nuclear morphology measurements.

Journal Article Gastroenterology · January 2011 Background & aimsPatients with Barrett's esophagus (BE) show increased risk of developing esophageal adenocarcinoma and are routinely examined using upper endoscopy with biopsy to detect neoplastic changes. Angle-resolved low coherence interferome ... Full text Cite

Development of angle-resolved low coherence interferometry for clinical detection of dysplasia.

Journal Article Journal of carcinogenesis · January 2011 This review covers the development of angle-resolved low coherence interferometry (a/LCI) from initial development through clinical application. In the first applications, the approach used a time-domain interferometry scheme and was validated using animal ... Full text Cite

Coherence imaging

Journal Article Optics InfoBase Conference Papers · December 1, 2010 This tutorial reviews coherence imaging approaches for biomedical applications. Subjects will include digital holography and optical coherence tomography modalities with an emphasis on basis of image formation, functional extensions and biological applicat ... Cite

New directions in interferometric phase microscopy of biological cell dynamics

Journal Article Optics InfoBase Conference Papers · December 1, 2010 Interferometric phase microscopy has the potential of becoming a widely-used tool for quantitative measurements of biological cells. We introduce the current state of the art, the open questions, and solutions experimentally developed in our laboratory. © ... Cite

Separating the scattering and absorption coefficients using the real and imaginary parts of the refractive index with low-coherence interferometry.

Journal Article Optics letters · September 2010 We present an analytical method that yields the real and imaginary parts of the refractive index (RI) from low-coherence interferometry measurements, leading to the separation of the scattering and absorption coefficients of turbid samples. The imaginary R ... Full text Open Access Cite

Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy.

Journal Article Biomedical optics express · August 2010 We apply wide-field interferometric microscopy techniques to acquire quantitative phase profiles of ventricular cardiomyocytes in vitro during their rapid contraction with high temporal and spatial resolution. The whole-cell phase profiles are analyzed to ... Full text Open Access Cite

Detection of early colorectal cancer development in the azoxymethane rat carcinogenesis model with Fourier domain low coherence interferometry.

Journal Article Biomedical optics express · August 2010 Fourier domain low coherence interferometry (fLCI) is an emerging optical technique used to quantitatively assess cell nuclear morphology in tissue as a means of detecting early cancer development. In this work, we use the azoxymethane rat carcinogenesis m ... Full text Cite

Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera.

Journal Article Optics letters · August 2010 We present a quantitative phase microscopy method that uses a Bayer mosaic color camera to simultaneously acquire off-axis interferograms in transmission mode at two distinct wavelengths. Wrapped phase information is processed using a two-wavelength algori ... Full text Open Access Cite

Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering.

Journal Article Optics express · July 5, 2010 We demonstrate accurate determination of the size and shape of spherical and spheroidal scatterers through inverse analysis of two-dimensional solid-angle and depth resolved backscattered light intensities. Intensity of scattered light is measured over a w ... Cite

Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering.

Journal Article Optics express · July 2010 We demonstrate accurate determination of the size and shape of spherical and spheroidal scatterers through inverse analysis of two-dimensional solid-angle and depth resolved backscattered light intensities. Intensity of scattered light is measured over a w ... Full text Open Access Cite

Review of the application of T-matrix calculations for determining the structure of cell nuclei with angle-resolved light scattering measurements

Journal Article IEEE Journal on Selected Topics in Quantum Electronics · July 1, 2010 Biomedical applications of light scattering have traditionally relied on Mie theory as a theoretical basis for analyzing experimental measurements. While this approach is computationally accessible, the use of Mie theory cannot always account for the spher ... Full text Cite

Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics.

Journal Article · May 20, 2010 We apply a wide-field quantitative phase microscopy technique based on parallel two-step phase-shifting on-axis interferometry to visualize live biological cells and microorganism dynamics. The parallel on-axis holographic approach is more efficient with c ... Open Access Cite

Measuring structural features using a dual window method for light scattering spectroscopy and Fourier-domain low coherence interferometry

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · May 7, 2010 Light scattering spectroscopy (LSS) and Fourier domain low coherence interferometry (fLCI) are used in combination with the dual window method (DW) to measure scattering features from a thick turbid sample. By processing with the DW method, the trade off t ... Full text Cite

Fourier-domain angle-resolved low coherence interferometry for clinical detection of dysplasia

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · May 7, 2010 Improved methods for detecting dysplasia, or pre-cancerous growth are a current clinical need, particularly in the esophagus. The currently accepted method of random biopsy and histological analysis provides only a limited examination of tissue in question ... Full text Cite

Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics.

Journal Article Journal of biomedical optics · May 2010 We introduce a new interferometric setup for single-exposure wide-field holographic phase imaging of highly dynamic biological samples. In this setup, the interferometric signal originates from a specially designed reflective interferometric chamber (InCh) ... Full text Cite

Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics.

Journal Article Applied optics · May 2010 We apply a wide-field quantitative phase microscopy technique based on parallel two-step phase-shifting on-axis interferometry to visualize live biological cells and microorganism dynamics. The parallel on-axis holographic approach is more efficient with c ... Full text Open Access Cite

Fiber-optic interferometric two-dimensional scattering-measurement system.

Journal Article Optics letters · May 2010 We present a fiber-optic interferometric system for measuring depth-resolved scattering in two angular dimensions using Fourier-domain low-coherence interferometry. The system is a unique hybrid of the Michelson and Sagnac interferometer topologies. The co ... Full text Open Access Cite

Deformation of stem cell nuclei by nanotopographical cues.

Journal Article Soft matter · April 2010 Cells sense cues in their surrounding microenvironment. These cues are converted into intracellular signals and transduced to the nucleus in order for the cell to respond and adapt its function. Within the nucleus, structural changes occur that ultimately ... Full text Open Access Cite

Measuring morphological features using light-scattering spectroscopy and Fourier-domain low-coherence interferometry.

Journal Article Optics letters · February 2010 We present measurements of morphological features in a thick turbid sample using light-scattering spectroscopy (LSS) and Fourier-domain low-coherence interferometry (fLCI) by processing with the dual-window (DW) method. A parallel frequency domain optical ... Full text Open Access Cite

Coherence imaging

Conference Optics InfoBase Conference Papers · January 1, 2010 This tutorial reviews coherence imaging approaches for biomedical applications. Subjects will include digital holography and optical coherence tomography modalities with an emphasis on basis of image formation, functional extensions and biological applicat ... Full text Cite

Quantitative phase microscopy of articular chondrocyte dynamics by wide-field digital interferometry.

Journal Article Journal of biomedical optics · January 2010 We experimentally implement label-free phase microscopy using wide-field digital interferometry (WFDI) techniques to retrieve quantitative volumetric data of articular chondrocyte dynamics. Using the scanless interferometric system, we visualize chondrocyt ... Full text Cite

Quantitative phase microscopy of live biological cell dynamics

Journal Article AIP Conference Proceedings · January 1, 2010 Interferometric phase microscopy of biological cell dynamics has the potential to provide a label-free quantitative tool for cell biology, as well as for medical diagnosis and monitoring. The current state of the art of this field, the open questions, and ... Full text Cite

Nonlinear osmotic properties of the cell nucleus

Journal Article Proceedings of the ASME Summer Bioengineering Conference 2009, SBC2009 · December 1, 2009 Full text Cite

Dynamic quantitative phase microscopy of biological cells

Journal Article Optics InfoBase Conference Papers · December 1, 2009 We introduce a new fast and accurate method for dynamic quantitative phase imaging of biological cells. The method enables imaging of sub-millisecond dynamic biological phenomena with sub-nanometer temporal stability. Initial experimental results are given ... Cite

Two-wavelength quantitative phase unwrapping of dynamic biological processes

Journal Article Optics InfoBase Conference Papers · December 1, 2009 We present two-wavelength phase unwrapping system designs on asynchronous and dynamic phase microscopy techniques developed in our laboratory. The use of two wavelengths in these systems enables quantitative phase imaging without 2p-ambiguities. © 2009 OSA ... Cite

Molecular imaging and darkfield microspectroscopy of live cells using gold plasmonic nanoparticles

Journal Article Laser and Photonics Reviews · November 23, 2009 Noble metal nanoparticles exhibit a plasmonic resonance that provides them with unique optical properties. The sensitivity of the plasmonic resonance to the surrounding dielectric environment has lead to the development of metal nanoparticles as the basis ... Full text Cite

Two-wavelength quantitative phase unwrapping of dynamic biological processes

Journal Article 2009 Conference on Lasers and Electro-Optics and 2009 Conference on Quantum Electronics and Laser Science Conference, CLEO/QELS 2009 · November 18, 2009 We present two-wavelength phase unwrapping system designs on asynchronous and dynamic phase microscopy techniques developed in our laboratory. The use of two wavelengths in these systems enables quantitative phase imaging without 2π-ambiguities. ©2009 Opti ... Cite

Dynamic quantitative phase microscopy of biological cells

Journal Article 2009 Conference on Lasers and Electro-Optics and 2009 Conference on Quantum Electronics and Laser Science Conference, CLEO/QELS 2009 · November 16, 2009 We introduce a new fast and accurate method for dynamic quantitative phase imaging of biological cells. The method enables imaging of sub-millisecond dynamic biological phenomena with sub-nanometer temporal stability. Initial experimental results are given ... Cite

Detecting precancerous lesions in the hamster cheek pouch using spectroscopic white-light optical coherence tomography to assess nuclear morphology via spectral oscillations.

Journal Article Journal of biomedical optics · November 2009 We have developed a novel dual-window approach for spectroscopic optical coherence tomography (OCT) measurements and applied it to probe nuclear morphology in tissue samples drawn from the hamster cheek pouch carcinogenesis model. The dual-window approach ... Full text Cite

Scanning fiber angle-resolved low coherence interferometry.

Journal Article Optics letters · October 2009 We present a fiber-optic probe for Fourier-domain angle-resolved low coherence interferometry for the determination of depth-resolved scatterer size. The probe employs a scanning single-mode fiber to collect the angular scattering distribution of the sampl ... Full text Cite

Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells.

Journal Article Optics express · August 2009 We present a phase-shifting interferometric technique for imaging live biological cells in growth media, while optimizing spatial resolution and enabling potential real-time measurement capabilities. The technique uses slightly-off-axis interferometry whic ... Full text Cite

Simultaneous molecular imaging of EGFR and HER2 using hyperspectral darkfield microscopy and immunotargeted nanoparticles

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · June 12, 2009 Epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor (HER2) contribute to the regulation of cell proliferation, and when jointly over-expressed are associated with several types of cancer. The ability to monitor both receptors ... Full text Cite

Optimizing Fourier-domain angle resolved low coherence interferometry for clinical use

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · June 12, 2009 Methods for the optimization of a/LCI for clinical use are presented. First, the use of the T-matrix light scattering model to simulate scattering from spheroidal particles is presented as a more appropriate simulation of cell nuclei scattering than the pr ... Full text Cite

Detecting neoplastic development in the hamster cheek pouch using Fourier Domain Low Coherence Interferometry

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · June 12, 2009 Fourier Domain Low Coherence Interferometry (fLCI) is an optical technique that recovers depth-resolved spectroscopic information about scatterers. The current fLCI system utilizes a white light Xe arc lamp source, a 4-f interferometer, and an imaging spec ... Full text Cite

T-matrix based inverse light scattering analysis using angle resolved low coherence interferometry

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · June 12, 2009 Inverse light scattering methods have been applied by several groups as a means to probe cellular structure in both clinical and scientific applications with sub-wavelength accuracy. These methods determine the geometric properties of tissue scatterers bas ... Full text Cite

Development of an integrated endoscopic device for multiplexed low coherence interferometry (LCI) measurements of microbicide gel thickness

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · June 8, 2009 Microbicide gels are topical products that have recently been developed to combat sexually transmitted diseases including HIV/AIDS. The extent of gel coverage, thickness, and structure are crucial factors in gel effectiveness. It is necessary to be able to ... Full text Cite

Dual window method for processing spectroscopic optical coherence tomography signals with high spectral and spatial resolution

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · May 5, 2009 The generation of spectroscopic optical coherence tomography (SOCT) signals suffers from an inherent trade off between spatial and spectral resolution. Here, we present a dual window (DW) method that uses two Gaussian windows to simultaneously obtain high ... Full text Cite

Rational selection of gold nanorod geometry for label-free plasmonic biosensors.

Journal Article ACS nano · April 2009 We present the development of an analytical model that can be used for the rational design of a biosensor based on shifts in the local surface plasmon resonance (LSPR) of individual gold nanoparticles. The model relates the peak wavelength of light scatter ... Full text Cite

Multiplexed low coherence interferometry instrument for measuring microbicide gel thickness distribution.

Journal Article Applied optics · April 2009 We present a Fourier-domain, multiplexed low coherence interferometry (LCI) instrument designed for application to intravaginal measurement of microbicidal gel distribution. Microbicide gels are topical products developed to combat sexually transmitted dis ... Full text Cite

Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nuclei.

Journal Article Applied optics · April 2009 Inverse light scattering analysis (ILSA) seeks to associate measured scattering properties with the most probable theoretical scattering distribution, making it a useful tool for assessing structure in biological materials. The accuracy of ILSA depends on ... Full text Cite

Dual window method for processing spectroscopic optical coherence tomography signals with simultaneously high spectral and temporal resolution.

Journal Article Optics express · April 2009 Current methods for analysis of spectroscopic optical coherence tomography (SOCT) signals suffer from an inherent tradeoff between time (depth) and frequency (wavelength) resolution. Here, we present a dual window (DW) method for reconstructing time freque ... Full text Cite

Molecular imaging and quantitative measurement of epidermal growth factor receptor expression in live cancer cells using immunolabeled gold nanoparticles.

Journal Article AJR Am J Roentgenol · April 2009 OBJECTIVE: The goal of this study was to assess whether immunolabeled nanoparticle biomarkers are comparable to fluorescent marker imaging in measuring epidermal growth factor receptor (EGFR) expression. MATERIALS AND METHODS: EGFR expression was quantifie ... Full text Link to item Cite

Reflectance spectroscopy.

Journal Article Gastrointestinal endoscopy clinics of North America · April 2009 Reflectance spectroscopy is an emerging technology which provides rapid and safe evaluation of tissue for dysplasia and ischemia. The probe-based devices can be passed through most endoscopes. Current applications include detection of dysplasia in Barrett' ... Full text Cite

Dual-interference-channel quantitative-phase microscopy of live cell dynamics.

Journal Article Optics letters · March 2009 We introduce and experimentally demonstrate a fast and accurate method for quantitative imaging of the dynamics of live biological cells. Using a dual-channel interferometric setup, two phase-shifted interferograms of nearly transparent biological samples ... Full text Cite

Nonlinear osmotic properties of the cell nucleus.

Journal Article Annals of biomedical engineering · March 2009 In the absence of active volume regulation processes, cell volume is inversely proportional to osmolarity, as predicted by the Boyle Van't Hoff relation. In this study, we tested the hypothesis that nuclear volume has a similar relationship with extracellu ... Full text Cite

Light scattering measurements of subcellular structure provide noninvasive early detection of chemotherapy-induced apoptosis.

Journal Article Cancer research · February 2009 We present a light scattering study using angle-resolved low coherence interferometry (a/LCI) to assess nuclear morphology and subcellular structure within MCF-7 cells at several time points after treatment with chemotherapeutic agents. Although the nuclea ... Full text Cite

Development of an integrated multiplexed low coherence interferometer and fluorescence clinical endoscope

Journal Article Optics InfoBase Conference Papers · January 1, 2009 The performance of a novel clinical endoscope with integrated multiplexed LCI and fluorescence measurements is evaluated. Feasibility and accuracy for measuring microbicidal gel distribution and thickness in the vaginal tract will be assessed. © 2009 Optic ... Cite

Development of an integrated multiplexed low coherence interferometer and fluorescence clinical endoscope

Conference Optics InfoBase Conference Papers · January 1, 2009 The performance of a novel clinical endoscope with integrated multiplexed LCI and fluorescence measurements is evaluated. Feasibility and accuracy for measuring microbicidal gel distribution and thickness in the vaginal tract will be assessed. © 2009 Optic ... Full text Cite

Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering.

Journal Article Optics letters · November 2008 We demonstrate an inverse light-scattering analysis procedure based on using the T-matrix method as a light-scattering model. We measure light scattered by in vitro cell monolayers using angle-resolved low-coherence interferometry (a/LCI) and compare the d ... Full text Cite

Photothermal optical coherence tomography of epidermal growth factor receptor in live cells using immunotargeted gold nanospheres.

Journal Article Nano letters · October 2008 Molecular imaging is a powerful tool for investigating disease processes and potential therapies in both in vivo and in vitro systems. However, high resolution molecular imaging has been limited to relatively shallow penetration depths that can be accessed ... Full text Cite

Application of Mie theory to assess structure of spheroidal scattering in backscattering geometries.

Journal Article Journal of the Optical Society of America. A, Optics, image science, and vision · August 2008 Inverse light scattering analysis seeks to associate measured scattering properties with the most probable theoretical scattering distribution. Although Mie theory is a spherical scattering model, it has been used successfully for discerning the geometry o ... Full text Cite

Parallel frequency-domain optical coherence tomography scatter-mode imaging of the hamster cheek pouch using a thermal light source.

Journal Article Optics letters · June 2008 We use a parallel frequency-domain optical coherence tomography (FDOCT) system to generate a scatter-mode image of the hamster cheek pouch epithelium. To our knowledge, this is the first optical coherence tomography (OCT) image of a biological sample obtai ... Full text Cite

Label-free, high-throughput measurements of dynamic changes in cell nuclei using angle-resolved low coherence interferometry.

Journal Article Biophysical journal · June 2008 Accurate measurements of nuclear deformation, i.e., structural changes of the nucleus in response to environmental stimuli, are important for signal transduction studies. Traditionally, these measurements require labeling and imaging, and then nuclear meas ... Full text Cite

Detection of structural and functional changes in biological materials using angle-resolved low coherence interferometry

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 21, 2008 A well-established method of assessing structure is inverse light scattering analysis. With inverse light scattering analysis, the measured scattering properties of a scatterer(s) are associated with the most probable scattering distribution predicted by a ... Full text Cite

Measuring neoplastic transformation in the hamster cheek pouch using Fourier domain low coherence interferometry

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 21, 2008 Fourier Domain Low Coherence Interferometry (fLCI) is a promising technique which combines the depth resolution of low coherence interferometry with the sensitivity of light scattering spectroscopy for probing the health of epithelial tissue layers. Our ne ... Full text Cite

Development of a clinical Fourier-domain angle resolved low coherence interferometry system for in vivo measurements

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 21, 2008 Improved methods for detecting dysplasia, or pre-cancerous growth are a current clinical need, particularly in the esophagus. The currently accepted method of random biopsy and histological analysis provides only a limited examination of tissue in question ... Full text Cite

Label-free plasmonic detection of biomolecular binding by a single gold nanorod.

Journal Article Analytical chemistry · February 2008 We report the use of individual gold nanorods as plasmonic transducers to detect the binding of streptavidin to individual biotin-conjugated nanorods in real time on a surface. Label-free detection at the single-nanorod level was performed by tracking the ... Full text Cite

Analyzing light scattering from aspherical nuclei for cell biology and clinical applications

Journal Article Optics InfoBase Conference Papers · January 1, 2008 We discuss new models of light scattering by aspherical cell nuclei and relate them to studies of cell mechanics and early cancer detection based on angle-resolve low coherence interferometry, an inverse light scattering method. © 2008 Optical Society of A ... Cite

Quantitative phase microscopy with multi-wavelength unwrapping and tomographic 3D reconstruction

Journal Article Optics InfoBase Conference Papers · January 1, 2008 Asynchronous digital holography has been developed for quantitative phase measurements, and will be extended to multi-wavelength phase unwrapping and 3D tomographic reconstruction. Results acquired from standard samples and also live cell samples will be p ... Cite

Quantitative phase microscopy with multi-wavelength unwrapping and tomographic 3D reconstruction

Journal Article Optics InfoBase Conference Papers · January 1, 2008 Asynchronous digital holography has been developed for quantitative phase measurements, and will be extended to multi-wavelength phase unwrapping and 3D tomographic reconstruction. Results acquired from standard samples and also live cell samples will be p ... Cite

Quantitative phase microscopy with multi-wavelength unwrapping and tomographic 3d reconstruction

Conference Biomedical Optics, BIOMED 2008 · January 1, 2008 Asynchronous digital holography has been developed for quantitative phase measurements, and will be extended to multi-wavelength phase unwrapping and 3D tomographic reconstruction. Results acquired from standard samples and also live cell samples will be p ... Full text Cite

Analyzing light scattering from aspherical nuclei for cell biology and clinical applications

Conference Optics InfoBase Conference Papers · January 1, 2008 We discuss new models of light scattering by aspherical cell nuclei and relate them to studies of cell mechanics and early cancer detection based on angle-resolve low coherence interferometry, an inverse light scattering method. © 2008 Optical Society of A ... Full text Cite

Molecular imaging of epidermal growth factor receptor in live cells with refractive index sensitivity using dark-field microspectroscopy and immunotargeted nanoparticles.

Journal Article Journal of biomedical optics · January 2008 We present a study using plasmonic nanoparticles (NPs) to image epidermal growth factor receptor (EGFR) in live cells. Through detailed analysis of the NP scattering spectra, we determine the intracellular refractive index (RI) within attoliter volumes ins ... Full text Cite

In situ nuclear morphology measurements using light scattering as biomarkers of neoplastic change in animal models of carcinogenesis.

Journal Article Disease markers · January 2008 Light scattering spectroscopy measurements can be used to determine the structure of tissue samples. Through refined data acquisition and signal processing techniques, quantitative nuclear morphology measurements may be obtained from light scattering data. ... Full text Cite

Review and recent development of angle-resolved low-coherence interferometry for detection of precancerous cells in human esophageal epithelium

Journal Article IEEE Journal on Selected Topics in Quantum Electronics · January 1, 2008 The combination of low-coherence interferometry with angle-resolved light scattering measurements has been shown to be a powerful method for determining the structure of cell nuclei within intact tissue samples. The nuclear morphology data have been used a ... Full text Cite

Multiplexed low-coherence interferometry instrument for measuring microbicide gel thickness distribution

Journal Article Biomedical Optics, BIOMED 2008 · January 1, 2008 We present a multiplexed low coherence interferometry (LCI) system for in vivo human vaginal imaging of microbicidal gel distribution. In vitro testing demonstrated high accuracy and linearity of LCI in measuring gel coating thickness up to 500um. © 2007 O ... Full text Cite

Modeling advances for measuring spheroidal geometries using angle-resolved low coherence interferometry

Journal Article Biomedical Optics, BIOMED 2008 · January 1, 2008 The relative efficacy of a modified Mie theory model and a T-matrix method model in determining the geometry of spheroidal scatterers is determined through comparison of scattering distributions of optical phantoms to these two models. © 2007 Optical Socie ... Full text Cite

Design and verification of an endoscopic pre-cancer detection system based on angle-resolved low coherence interferometry (a/LCI)

Journal Article Biomedical Optics, BIOMED 2008 · January 1, 2008 This paper presents the design and implementation of a portable endoscopic a/LCI system for clinical pre-cancer detection through accurate sizing of cell nuclei. System performance is evaluated in ex vivo measurements. ©2007 Optical Society of America. ... Full text Cite

A comparison of mie theory and the t-matrix method for estimating the size of cell nuclei

Journal Article Biomedical Optics, BIOMED 2008 · January 1, 2008 We investigate the accuracy of inverse light scattering cell nuclei size predictions derived from Mie theory with the T-matrix method over a range of aspherical nuclei relevant to cancer detection and monitoring. © 2007 Optical Society of America. ... Full text Cite

Darkfield microspectroscopy: From single nanoparticle biosensing to live cell molecular imaging

Journal Article Biomedical Optics, BIOMED 2008 · January 1, 2008 Darkfield microspectroscopy enables detailed studies of the plasmonic features of noble metal nanoparticles. Shifts of the plasmon resonance of individual nanoparticles can be exploited for sensing applications or to achieve molecular imaging using targete ... Full text Cite

Development of a novel hyperspectral darkfield microscopy system for characterization of nanoparticle sensors

Journal Article Biomedical Optics, BIOMED 2008 · January 1, 2008 We present a novel hyperspectral darkfield microscopy scheme that utilizes an epiillumination light train for improved characterization of the sensing capacity of noble metal nanoparticles. Validation experiments are presented to characterize the new syste ... Full text Cite

Quantitative phase microscopy with asynchronous digital holography system

Journal Article Conference on Lasers and Electro-Optics, 2007, CLEO 2007 · December 1, 2007 We demonstrate a new method of measuring quantitative phase in biological materials. The method utilizes asynchronous digital holography, which uses a moving fringe created by acousto-optic modulators. Results are demonstrated on live cell samples. © OSA. ... Full text Cite

Molecular imaging of EGFR expression in live cancer cells using immunotargeted nanoparticles

Journal Article Conference on Lasers and Electro-Optics, 2007, CLEO 2007 · December 1, 2007 Using molecular imaging of immunolabeled plasmonic nanoparticles bound by cell surface receptors, we compare epidermal growth factor receptor expression, an indicator of cancerous activity, of both human epithelial carcinoma and brain tumor cell lines. ©OS ... Full text Cite

Development of a pre-clinical Fourier domain low coherence interferometry system

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · August 31, 2007 Fourier domain low coherence interferometry (fLCI) is an optical technique which combines the depth resolution of low coherence interferometry with the sensitivity of light scattering spectroscopy. The fLCI system uses a white light source in a modified Mi ... Full text Cite

Development of a portable frequency-domain angle-resolved low coherence interferometry system

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · August 31, 2007 Improved methods for detecting dysplasia, or pre-cancerous growth, are a current clinical need. Random biopsy and subsequent diagnosis through histological analysis is the current gold standard in endoscopic surveillance for dysplasia. However, this approa ... Full text Cite

Progress in Biomedical Optics and Imaging - Proceedings of SPIE: Introduction

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · August 31, 2007 Cite

Temporal coherence and time-frequency distributions in spectroscopic optical coherence tomography.

Journal Article Journal of the Optical Society of America. A, Optics, image science, and vision · August 2007 Traditional analysis of spectroscopic optical coherence tomography (SOCT) signals is limited by an uncertainty relationship between time (depth) and frequency (wavelength). The use of a bilinear time-frequency distribution for analysis, such as those that ... Full text Cite

Application of Mie theory to determine the structure of spheroidal scatterers in biological materials.

Journal Article Optics letters · May 2007 We present here the results of a numerical study on light scattering from nonspherical particles with relevance to detecting precancerous states in epithelial tissues. In previous studies of epithelial cell nuclei, the experimental light scattering data ha ... Full text Cite

Polarization effects on scatterer sizing accuracy analyzed with frequency-domain angle-resolved low-coherence interferometry.

Journal Article Applied optics · April 2007 Angle-resolved low-coherence interferometry (a/LCI) enables us to make depth-resolved measurements of scattered light that can be used to recover subsurface structural information such as the size of cell nuclei. Endoscopic frequency-domain a/LCI (fa/LCI) ... Full text Cite

Analysis of total uncertainty in spectral peak measurements for plasmonic nanoparticle-based biosensors.

Journal Article Applied optics · April 2007 One goal of recent research on plasmonic nanoparticle-based sensors is maximizing nanoparticle sensitivity or shift of resonance peak wavelength per refractive index change. Equally important is a measurement system's peak location uncertainty or shift res ... Full text Cite

In situ detection of nuclear atypia in Barrett's esophagus by using angle-resolved low-coherence interferometry.

Journal Article Gastrointest Endosc · March 2007 BACKGROUND: Monitoring of patients with Barrett's esophagus (BE) for dysplasia, currently done by systematic biopsy, can be improved through increasing the proportion of at-risk tissue examined. OBJECTIVE: Optical biopsy techniques, which do not remove the ... Full text Link to item Cite

Quantitative phase microscopy with asynchronous digital holography.

Journal Article Optics express · March 2007 We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acousto-optic modulators to execute phase-shifting interferometry ... Full text Cite

In situ assessment of intraepithelial neoplasia in hamster trachea epithelium using angle-resolved low-coherence interferometry.

Journal Article Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · February 2007 Optical spectroscopy was used to evaluate the transformation of nuclear morphology associated with intraepithelial neoplasia in an animal model of carcinogenesis. In this pilot study, we have assessed the capability of angle-resolved low-coherence interfer ... Full text Cite

Molecular imaging and microspectroscopy of live cells using immunotargeted nanoparticles

Journal Article Optics InfoBase Conference Papers · January 1, 2007 We compare the expression of EGFR by epithelial carcinoma and neuroepithelial tumor cell lines, using hyperspectral darkfield microscopy to measure the scattering spectra of immunolabeled plasmonic nanoparticles bound by cell surface receptors. © 2007 OSA. ... Cite

Molecular imaging of EGFR expression in live cancer cells using immunotargeted nanoparticles

Journal Article Optics InfoBase Conference Papers · 2007 Using molecular imaging of immunolabeled plasmonic nanoparticles bound by cell surface receptors, we compare epidermal growth factor receptor expression, an indicator of cancerous activity, of both human epithelial carcinoma and brain tumor cell lines. © 2 ... Cite

Quantitative phase microscopy with asynchronous digital holography system

Journal Article Optics InfoBase Conference Papers · 2007 We demonstrate a new method of measuring quantitative phase in biological materials. The method utilizes asynchronous digital holography, which uses a moving fringe created by acousto-optic modulators. Results are demonstrated on live cell samples © 2007 O ... Cite

Molecular imaging and microspectroscopy of live cells using immunotargeted nanoparticles

Conference Optics InfoBase Conference Papers · January 1, 2007 We compare the expression of EGFR by epithelial carcinoma and neuroepithelial tumor cell lines, using hyperspectral darkfield microscopy to measure the scattering spectra of immunolabeled plasmonic nanoparticles bound by cell surface receptors. © 2007 OSA. ... Full text Cite

Workshop on imaging science development for cancer prevention and preemption.

Journal Article Cancer Biomark · 2007 The concept of intraepithelial neoplasm (IEN) as a near-obligate precursor of cancers has generated opportunities to examine drug or device intervention strategies that may reverse or retard the sometimes lengthy process of carcinogenesis. Chemopreventive ... Full text Link to item Cite

Tissue self-affinity and polarized light scattering in the born approximation: a new model for precancer detection.

Journal Article Physical review letters · September 2006 Light scattered from biological tissues can exhibit an inverse power law spectral component. We develop a model based on the Born approximation and von Karman (self-affine) spatial correlation of submicron tissue refractive index to account for this. The m ... Full text Cite

Epi-illumination through the microscope objective applied to darkfield imaging and microspectroscopy of nanoparticle interaction with cells in culture.

Journal Article Optics express · July 2006 Existing darkfield illumination schemes are incompatible with many types of samples and/or procedures. We present a darkfield epi-illumination scheme which addresses these incompatibilities by providing illumination through the imaging objective. We valida ... Full text Cite

Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry.

Journal Article Journal of biomedical optics · May 2006 Angle-resolved low coherence interferometry (a/LCI) enables depth-resolved measurements of scattered light that can be used to recover subsurface structural information, such as the size of cell nuclei. Measurements of nuclear morphology, however, can be c ... Full text Cite

Label-free measurement of microbicidal gel thickness using low-coherence interferometry.

Journal Article Journal of biomedical optics · March 2006 Spectral-domain low-coherence interferometry (LCI) was used to measure the thickness of microbicidal gels applied to a cylindrical calibration test socket. Microbicides are topical formulations containing active ingredients targeted to inhibit specific pat ... Full text Cite

Fourier-domain angle-resolved low coherence interferometry through an endoscopic fiber bundle for light-scattering spectroscopy.

Journal Article Optics letters · March 2006 We present a novel endoscopic fiber bundle probe incorporated in a Fourier-domain angle-resolved low coherence interferometry system for the measurement of depth-resolved angular scattering distributions to permit the determination of scatterer size via el ... Full text Cite

Measurement system for the high-throughput characterization of metal nanoparticles for biosensors

Journal Article Optics InfoBase Conference Papers · January 1, 2006 We present a system for the rapid characterization of nanoparticles for biosensors, providing concurrent atomic force microscopy and scattering spectra of individual nanoparticles and simultaneous spectra from various nanostructures created by electron bea ... Cite

Optical scattering of confocal laser scanning reflectance microscopy in turbid media

Journal Article Optics InfoBase Conference Papers · January 1, 2006 We present quantitative analysis of optical scattering in confocal reflectance microscopy as a function of numerical aperture and scattering coefficient. Data is collected from samples of intralipid of varying concentration with objectives up to 1.4NA. © 2 ... Cite

Endoscopic fourier-domain angle-resolved low coherence interferometry for assessing nuclear morphology in human epithelial tissues

Journal Article Optics InfoBase Conference Papers · January 1, 2006 Endoscopic Fourier-domain angle-resolved low coherence interferometry has been developed for depth resolved light scattering spectroscopy. System capabilities are demonstrated by determining the nuclei size in tissue phantoms, cell cultures and ex vivo hum ... Cite

Epi-illumination darkfield through a microscope objective for imaging and spectral analysis of nanoparticle interaction with cells in culture

Journal Article Optics InfoBase Conference Papers · January 1, 2006 Existing darkfield illumination schemes are incompatible with many types of samples and/or procedures. We present a darkfield scheme which addresses these incompatibilities. We then apply the scheme to evaluate nanoparticles binding cancer cells in culture ... Cite

Improved design of a heterodyne angle-resolved low coherence interferometry system used for new chemoprevention and carcinogenesis studies

Journal Article Optics InfoBase Conference Papers · January 1, 2006 Improvements have been made in existing angle-resolved interferometry system to enable greater accuracy and angular range for use in new chemopreventive/carcinogenic studies. © 2005 Optical Society of America. ... Cite

Improved simulations for measuring microbicidal gel thickness using low-coherence interferometry

Journal Article Optics InfoBase Conference Papers · January 1, 2006 Spectral-domain low coherence interferometry is used to measure the thickness of microbicidal gels as applied to realistic tissue phantoms. Reflections originating between the gel and phantom are analyzed to give thickness measurements. © 2005 Optical Soci ... Cite

Multi-layer scattering tissue phantom for assessing angle-resolved low coherence interferometryprecancer diagnostic technique

Journal Article Optics InfoBase Conference Papers · January 1, 2006 Scattering tissue phantoms are developed for assessment of promising optical precancer diagnostic technique. The tissue phantoms provide a valuable evaluation method for the low-coherence interferometry technique. Size determination results and phantom rel ... Cite

Quantitative characterization of spectrograph entrance slit width on roll-off of fourier domain optical coherence tomography signals

Journal Article Optics InfoBase Conference Papers · January 1, 2006 The effect of spectrograph slit width on the depth sensitivity of Fourier domain optical coherence tomography is investigated. A relationship between the two is derived mathematically and supported experimentally. © 2005 Optical Society of America. ... Cite

Sensitivity analysis of detecting plasmon resonance spectral shifts for nanoparticle based biosensors

Journal Article Optics InfoBase Conference Papers · January 1, 2006 Shifts in the plasmon resonance of single nanoparticles are being investigated for biosensing applications. A systematic analysis of the factors which influence uncertainties in the spectral data and a method for optimizing acquisition are presented. © 200 ... Cite

Improved design of a heterodyne angle-resolved low coherence interferometry system used for new chemoprevention and carcinogenesis studies

Conference Optics InfoBase Conference Papers · January 1, 2006 Improvements have been made in existing angle-resolved interferometry system to enable greater accuracy and angular range for use in new chemopreventive/carcinogenic studies. © 2005 Optical Society of America. ... Full text Cite

Epi-illumination darkfield through a microscope objective for imaging and spectral analysis of nanoparticle interaction with cells in culture

Conference Optics InfoBase Conference Papers · January 1, 2006 Existing darkfield illumination schemes are incompatible with many types of samples and/or procedures. We present a darkfield scheme which addresses these incompatibilities. We then apply the scheme to evaluate nanoparticles binding cancer cells in culture ... Full text Cite

Improved simulations for measuring microbicidal gel thickness using low-coherence interferometry

Conference Optics InfoBase Conference Papers · January 1, 2006 Spectral-domain low coherence interferometry is used to measure the thickness of microbicidal gels as applied to realistic tissue phantoms. Reflections originating between the gel and phantom are analyzed to give thickness measurements. © 2005 Optical Soci ... Full text Cite

Quantitative characterization of spectrograph entrance slit width on roll-off of fourier domain optical coherence tomography signals

Conference Optics InfoBase Conference Papers · January 1, 2006 The effect of spectrograph slit width on the depth sensitivity of Fourier domain optical coherence tomography is investigated. A relationship between the two is derived mathematically and supported experimentally. © 2005 Optical Society of America. ... Full text Cite

Sensitivity analysis of detecting plasmon resonance spectral shifts for nanoparticle based biosensors

Conference Optics InfoBase Conference Papers · January 1, 2006 Shifts in the plasmon resonance of single nanoparticles are being investigated for biosensing applications. A systematic analysis of the factors which influence uncertainties in the spectral data and a method for optimizing acquisition are presented. © 200 ... Full text Cite

Endoscopic fourier-domain angle-resolved low coherence interferometry for assessing nuclear morphology in human epithelial tissues

Conference Optics InfoBase Conference Papers · January 1, 2006 Endoscopic Fourier-domain angle-resolved low coherence interferometry has been developed for depth resolved light scattering spectroscopy. System capabilities are demonstrated by determining the nuclei size in tissue phantoms, cell cultures and ex vivo hum ... Full text Cite

Optical scattering of confocal laser scanning reflectance microscopy in turbid media

Journal Article 2005 Conference on Lasers and Electro-Optics, CLEO · December 1, 2005 We present quantitative analysis of optical scattering in confocal reflectance microscopy as a function of numerical aperture and scattering coefficient. Data is collected from samples of intralipid of varying concentration with objectives up to 1.4NA. © 2 ... Cite

Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry.

Journal Article Journal of biomedical optics · September 2005 Angle-resolved low-coherence interferometry (a/LCI) is used to obtain quantitative, depth-resolved nuclear morphology measurements. We compare the average diameter and texture of cell nuclei in rat esophagus epithelial tissue to grading criteria establishe ... Full text Cite

Nuclear morphology measurements using Fourier domain low coherence interferometry

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · July 21, 2005 We have developed Fourier domain low coherence interferometry (fLCI), a novel optical interferometry method for obtaining depth-resolved spectral information, specifically for the purpose of determining the size of scatterers by measuring their elastic sca ... Full text Cite

Rapid angle-resolved low coherence interferometry measurements

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · July 21, 2005 We have developed a novel angle-resolved low coherence interferometry scheme for rapid measurement of depth-resolved angular scattering distributions. These measurements enable the determination of scatterer size via elastic scattering properties. The sche ... Full text Cite

Coherent light scattering by in vitro cell arrays observed with angle-resolved low coherence interferometry

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · July 21, 2005 Angle-resolved low coherence interferometry enables depth-resolved measurements of scattered light. The scattered light measurements can be used to recover structural information from sub-surface layers, such as the size of cell nuclei. Measurements of nuc ... Full text Cite

Progress in Biomedical Optics and Imaging - Proceedings of SPIE: Introduction

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · July 21, 2005 Cite

Nuclear morphology measurements using Fourier domain low coherence interferometry.

Journal Article Optics express · June 2005 We present a new common path configuration Fourier domain low coherence interferometry (fLCI) optical system and demonstrate its capabilities by presenting results which determine the size of cell nuclei in a monolayer of T84 epithelial cells. The optical ... Full text Cite

Low-cost, scalable laser scanning module for real-time reflectance and fluorescence confocal microscopy.

Journal Article Applied optics · April 2005 We present a low-cost, high-speed, retrofitted laser scanning module for microscopy. The cage-mounted system, with various available fiber-coupled sources, offers a real-time imaging alternative to costly commercial systems with capabilities for convention ... Full text Cite

Substrate effect on refractive index dependence of plasmon resonance for individual silver nanoparticles observed using darkfield microspectroscopy.

Journal Article Optics express · April 2005 We use optical darkfield micro-spectroscopy to characterize the plasmon resonance of individual silver nanoparticles in the presence of a substrate. The optical system permits multiple individual nanoparticles to be identified visually for simultaneous spe ... Full text Cite

Improved interferometric detection of scattered light with a 4f imaging system.

Journal Article Applied optics · April 2005 We analyze the performance of three imaging systems to detect near-forward scattered light interferometrically by using a Mach-Zehnder geometry. The alignment of each system is demonstrated by measurement of the heterodyne efficiency and correlation of the ... Full text Cite

Harmonic phase-dispersion microscope with a Mach-Zehnder interferometer.

Journal Article Applied optics · March 2005 Harmonic phase-dispersion microscopy (PDM) is a new imaging technique in which contrast is provided by differences in refractive index at two harmonically related wavelengths. We report a new configuration of the harmonic phase-dispersion microscope in a M ... Full text Cite

Low-coherence light-scattering calculations for polydisperse size distributions.

Journal Article Journal of the Optical Society of America. A, Optics, image science, and vision · February 2005 The calculation of angular light-scattering distributions is considered for low-coherence light incident on a polydisperse particle size distribution of scatterers. As low-coherence light is now commonly used in interferometry schemes when applied to biome ... Full text Cite

Measurement system for the high-throughput characterization of metal nanoparticles for biosensors

Journal Article Optics InfoBase Conference Papers · January 1, 2005 We present a system for the rapid characterization of nanoparticles for biosensors, providing concurrent atomic force microscopy and scattering spectra of individual nanoparticles and simultaneous spectra from various nanostructures created by electron bea ... Cite

Optical scattering of confocal laser scanning reflectance microscopy in turbid media

Journal Article Optics InfoBase Conference Papers · January 1, 2005 We present quantitative analysis of optical scattering in confocal reflectance microscopy as a function of numerical aperture and scattering coefficient. Data is collected from samples of intralipid of varying concentration with objectives up to 1.4NA. © 2 ... Cite

Measurement system for the high-throughput characterization of metal nanoparticles for biosensors

Journal Article 2005 Conference on Lasers and Electro-Optics, CLEO · January 1, 2005 We present a system for the rapid characterization of nanoparticles for biosensors, providing concurrent atomic force microscopy and scattering spectra of individual nanoparticles and simultaneous spectra from various nanostructures created by electron bea ... Full text Cite

Rapid, depth-resolved light scattering measurements using Fourier domain, angle-resolved low coherence interferometry.

Journal Article Optics express · December 2004 We present a novel angle-resolved low coherence interferometry scheme for rapid measurement of depth-resolved angular scattering distributions to enable determination of scatterer size via elastic scattering properties. Depth resolution is achieved using a ... Full text Cite

Quantitative phase imaging using actively stabilized phase-shifting low-coherence interferometry.

Journal Article Optics letters · October 2004 We describe a quantitative phase-imaging interferometer in which phase shifting and noise cancellation are performed by an active feedback loop using a reference laser. Depth gating via low-coherence light allows phase measurement from weakly reflecting bi ... Full text Cite

Detecting pre-cancerous cells using angle-resolved low coherence interferometry

Conference Optics InfoBase Conference Papers · January 1, 2004 Angle-resolved low coherence interferometry is used to detect pre-cancerous basal epithelial cells in intact, unstained animal tissues via quantitative nuclear morphology measurements. Recent results are presented which demonstrate the ability to assess th ... Cite

Low-cost, highly-adaptable, retrofitted laser scanning module for real-time reflectance and confocal fluorescence microscopy

Conference Optics InfoBase Conference Papers · January 1, 2004 We present a low-cost, high-speed, retrofitted laser scanning module for microscopy. This fiber-coupled source, cage-mounted system offers a real-time imaging alternative to costly commercial systems and can be easily adapted for confocal and fluorescence ... Cite

Experimental calibration of a new angle-resolved low coherence interferometry system

Conference Optics InfoBase Conference Papers · January 1, 2004 We describe results of calibration experiments using a new angle-resolved low coherence interferometry system. Light scattered from a polystyrene microsphere sample are compared with Mie Theory predictions to determine the size of the particles. ... Cite

Real-time angle-resolved low-coherence interferometry for detecting pre-cancerous cells

Conference Optics InfoBase Conference Papers · January 1, 2004 Angle-resolved low coherence interferometry is used to detect pre-cancerous basal epithelial cells in intact, unstained animal tissues via quantitative nuclear morphology measurements. Recent results are presented which demonstrate the ability to assess th ... Cite

Determining nuclear morphology using an improved angle-resolved low coherence interferometry system.

Journal Article Optics express · December 2003 We outline the process for determining the morphology of subsurface epithelial cell nuclei using depth-resolved light scattering measurements. The measurements are accomplished using a second generation angle-resolved low coherence interferometry system. T ... Full text Cite

Instrumentation for multi-modal spectroscopic diagnosis of epithelial dysplasia.

Journal Article Technology in cancer research & treatment · December 2003 Reflectance and fluorescence spectroscopies have shown great promise for early detection of epithelial dysplasia. We have developed a clinical reflectance spectrofluorimeter for multimodal spectroscopic diagnosis of epithelial dysplasia. This clinical inst ... Full text Cite

Fourier-domain low-coherence interferometry for light-scattering spectroscopy.

Journal Article Optics letters · July 2003 We present a novel method for obtaining depth-resolved spectra for determining scatterer size through elastic-scattering properties. Depth resolution is achieved with a white-light source in a Michelson interferometer with the mixed signal and reference fi ... Full text Cite

In situ detection of neoplastic transformation and chemopreventive effects in rat esophagus epithelium using angle-resolved low-coherence interferometry.

Journal Article Cancer research · July 2003 We present a quantitative study of the nuclear morphometry of epithelial cells in an animal model of esophageal carcinogenesis. Changes in the size and texture of cell nuclei as a result of neoplastic transformation and chemopreventive action are observed ... Cite

A reflectance spectrofluorimeter for real-time spectral diagnosis of disease

Journal Article Review of Scientific Instruments · November 1, 2002 Full text Cite

Determination of particle size by using the angular distribution of backscattered light as measured with low-coherence interferometry.

Journal Article Journal of the Optical Society of America. A, Optics, image science, and vision · April 2002 We employ a novel interferometer to measure the angular distribution of light backscattered by a turbid medium. Through comparison of the measured data with the predictions of Mie theory, we are able to determine the size of the scatterers comprising the m ... Full text Cite

Cellular organization and substructure measured using angle-resolved low-coherence interferometry.

Journal Article Biophysical journal · April 2002 We measure the organization and substructure of HT29 epithelial cells in a monolayer using angle-resolved low-coherence interferometry. This new technique probes cellular structure by measuring scattered light, as in flow cytometry, but offers an advantage ... Full text Cite

2π ambiguity-free optical distance measurement with subnanometer precision with a novel phase-crossing low-coherence interferometer

Journal Article Optics Letters · January 15, 2002 We report a highly accurate phase-based technique for measuring arbitrarily long optical distance with sub-nanometer precision. The method employs a Michelson interferometer with a pair of harmonically related light sources, one cw and the other low cohere ... Full text Cite

Structure and organization of cellular components measured in neoplastic tissues using angular low coherence interferometry

Conference Optics InfoBase Conference Papers · January 1, 2002 We use angular low coherence interferometry (a/LCI) to measure the size of cell nuclei and organization of smaller cell components for sub-surface layers in living tissues, demonstrating its applicability for detecting early stages of neoplasia. ... Cite

Measuring Cellular Structure at Submicron Scale with Scattering Angle Sensitive Light Scattering Spectroscopy

Conference Optics InfoBase Conference Papers · January 1, 2002 We present an innovative technique for simultaneous measurement of the angular and spectral distributions of light backscattered by biological cells and tissues. The intensities of light scattered to different angular regions are due to scatterers of diffe ... Cite

Diffuse reflectance spectroscopy as an in vivo tool for characterizing changes in tissue organization during neoplastic development

Conference Optics InfoBase Conference Papers · January 1, 2002 Diffuse reflectance spectra are analyzed using a light-diffusion-theory-based model, which assumes that tissue scattering can be simulated by Mie scattering with an inverse power-law particle size distribution. Diagnostic information related to tissue orga ... Cite

2π ambiguity-free optical distance measurement with subnanometer precision with a novel phase-crossing low-coherence interferometer

Journal Article Opt. Lett. (USA) · 2002 We report a highly accurate phase-based technique for measuring arbitrarily long optical distance with subnanometer precision. The method employs a Michelson interferometer with a pair of harmonically related light sources, one cw and the other low coheren ... Cite

The study of cell dynamics with a novel phase referenced low coherence interferometer with sub-wavelength and sub-hertz sensitivity

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2002 We report the use of a highly sensitive phase based motion measurement technique to study the correlation of cellular metabolic rate with cellular motions. The technique is based on a modified Michelson interferometer with a composite laser beam of 1550 nm ... Full text Cite

Imaging and measurement of cell structure and organization with submicron accuracy using light scattering spectroscopy

Journal Article Proceedings of SPIE-The International Society for Optical Engineering · January 1, 2002 Light scattering spectroscopy (LSS) is a promising optical technique developed for quantitative characterization of tissue morphology as well as in vivo detection and diagnosis of disease such as early cancer. LSS employs a wavelength dependent component o ... Full text Cite

Measuring cellular structure at submicrometer scale with light scattering spectroscopy

Journal Article IEEE Journal on Selected Topics in Quantum Electronics · November 1, 2001 We present a novel instrument for imaging the angular distributions of light backscattered by biological cells and tissues. The intensities in different regions of the image are due to scatterers of different sizes. We exploit this to study scattering from ... Full text Cite

Phase dispersion optical tomography applied to study sub-cellular motions

Journal Article Conference on Lasers and Electro-Optics Europe - Technical Digest · October 8, 2001 Phase dispersion optical tomography (PDOT) technique that eliminates interferometer jitter noise was analyzed. The input light was a pair of overlapped beams of laser light at wavelength λ and 2λ. The Gelatin and water layer sandwiched between two cover sl ... Cite

Path-length-resolved dynamic light scattering: Modeling the transition from single to diffusive scattering

Journal Article Applied Optics · August 20, 2001 Dynamic light-scattering spectroscopy is used to study Brownian motion within highly scattering samples. The fluctuations of the light field that is backscattered by a suspension of polystyrene microspheres are measured as power spectra by use of low-coher ... Full text Cite

Phase-referenced interferometer with subwavelength and subhertz sensitivity applied to the study of cell membrane dynamics

Journal Article Optics Letters · August 15, 2001 We report a highly sensitive means of measuring cellular dynamics with a novel interferometer that can measure motional phase changes. The system is based on a modified Michelson interferometer with a composite laser beam of 1550-nm low-coherence light and ... Full text Cite

Phase-dispersion optical tomography

Journal Article Optics Letters · May 15, 2001 We report on phase-dispersion optical tomography, a new imaging technique based on phase measurements using low-coherence interferometry. The technique simultaneously probes the target with fundamental and second-harmonic light and interferometrically meas ... Full text Cite

Measurement of angular distributions by use of low-coherence interferometry for light-scattering spectroscopy

Journal Article Optics Letters · March 15, 2001 We present a novel interferometer for measuring angular distributions of backscattered light. The new system exploits a low-coherence source in a modified Michelson interferometer to provide depth resolution, as in optical coherence tomography, but include ... Full text Cite

Measurement of the anomalous phase velocity of ballistic light in a random medium by use of a novel interferometer

Journal Article Optics Letters · February 15, 2001 Ballistic light, i.e., radiation that propagates undeflected through a turbid medium, undergoes a small change in phase velocity and exhibits unusual dispersion because of its wave nature. We use a novel highly sensitive differential phase optical interfer ... Full text Cite

Interferometric phase-based dual wavelength tomography

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 We describe our phase-sensitive interferometry technique implemented as phase dispersion microscopy (PDM)/optical tomography (PDOT). The technique is based on measuring the phase difference between fundamental and second harmonic low coherence light in a n ... Full text Cite

Angular light scattering studies using low-coherence interferometry

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 A modified Michelson interferometer is used to measure path-length resolved angular distributions of light backscattered by turbid media. The path length resolution is obtained by exploiting the coherence properties of a broadband source. The angular distr ... Full text Cite

Phase-referenced interferometer with subwavelength and subhertz sensitivity

Journal Article Optics and Photonics News · January 1, 2001 A low coherence phase-referenced interferometer (PRI) that can be used to measure slow and small movements with the objective of studying subcellular dynamics was presented. The use of low coherence light in the interferometer provides depth resolution and ... Full text Cite

Multiexcitation fluorescence and reflectance spectroscopy as a real time guide to biopsy in vivo

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 A compact excitation-emission spectrofluorimeter system used to acquire tissue fluorescence and reflectance with fiber optic light delivery and collection is presented. Ten dye cells are pumped by a XeCl excimer laser, creating laser excitation from 308 nm ... Full text Cite

Interferometric phase-dispersion microscopy

Journal Article Optics Letters · October 15, 2000 We describe a new scanning microscopy technique, phase-dispersion microscopy (PDM). The technique is based on measuring the phase difference between the fundamental and the second-harmonic light in a novel interferometer. PDM is highly sensitive to subtle ... Full text Cite

Time-resolved phase-space distributions for light backscattered from a disordered medium.

Journal Article Physical review letters · July 2000 We demonstrate time-resolved measurement of optical phase-space distributions as a new probe for investigating the propagation of light in disordered media. Phase-space techniques measure the joint transverse position and momentum distribution of the scatt ... Full text Cite

Feasibility of field-based light scattering spectroscopy.

Journal Article Journal of biomedical optics · April 2000 Light scattering spectroscopy (LSS) is a new technique capable of accurately measuring the features of nuclei and other cellular organelles in situ. We present the considerations required to implement and interpret field-based detection in LSS, where the s ... Full text Cite

Path-length-resolved dynamic light scattering: modeling the transition from single to diffusive scattering

Conference Optics InfoBase Conference Papers · January 1, 2000 We interferometrically measure path-length resolved power spectra of light backscattered by turbid media undergoing Brownian motion. The results are compared to a theoretical treatment, which analyzes the total power spectra by the number of scattering eve ... Cite

Time-resolved optical phase space distributions for coherent backscatter

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2000 We explore enhanced backscatter from a random medium using time-resolved optical phase space measurement, i.e. measurement of joint position and momentum (x, p) distributions of the light field as a function of propagation time in the medium. Enhanced back ... Cite

Wigner phase space distributions and coherence tomography

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2000 We demonstrate the measurement of path-length-resolved optical phase space distributions as a new framework for exploring the evolution of optical coherence in a turbid medium. This method measures joint transverse position and momentum (i.e., angle) distr ... Cite

Heterodyne measurement of Wigner distributions for classical optical fields.

Journal Article Optics letters · October 1999 We demonstrate a two-window heterodyne method for measuring the x-p cross correlation, ??(*)(x)? (p)?, of an optical field ? for transverse position x and transverse momentum p. This scheme permits independent control of the x and p resolution. A simple li ... Full text Cite

Characterizing the Coherence of Broadband Sources using Optical Phase Space Contours.

Journal Article Journal of biomedical optics · October 1999 Advances in optical coherence tomography (OCT) rely on the availability of broadband light that is spatially coherent. We present a technique to characterize coherence properties of broadband light using optical phase-space contours in transverse momentum ... Full text Cite

Optical phase-space distributions for low-coherence light.

Journal Article Optics letters · September 1999 Using a novel heterodyne technique, we measure optical phase-space distributions in momentum and position for low-coherence light. Quantitative information is obtained simultaneously about the longitudinal and the transverse coherence properties as well as ... Full text Cite

Measurement of smoothed Wigner phase space distributions for coherence tomography

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1999 We use heterodyne detection to characterize the Wigner phase space distribution W (x, p) of an optical field in position x and momentum p. This method yields smoothed Wigner phase space distributions directly as contour plots and can be applied with either ... Full text Cite

Measurement of smoothed Wigner phase space distributions for coherence tomography

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1999 We use heterodyne detection to characterize the Wigner phase space distribution W(x, p) of an optical field in position x and momentum p. This method yields smoothed Wigner phase space distributions directly as contour plots and can be applied with either ... Cite

Measurement of smoothed Wigner phase-space distributions for small-angle scattering in a turbid medium.

Journal Article Journal of the Optical Society of America. A, Optics, image science, and vision · July 1998 We study Wigner phase-space distributions W (x, p) in position (x) and momentum (p) for light undergoing multiple small-angle scattering in a turbid medium. Smoothed Wigner phase-space distributions are measured by using a heterodyne technique that achieve ... Full text Cite

Measurement of Smoothed Wigner Phase Space Distributions in Multiple Scattering Media

Conference Optics InfoBase Conference Papers · January 1, 1998 We use a simple heterodyne method to measure smoothed Wigner phase space distributions for light transmitted through a turbid medium. A three component distribution is seen to emerge from the sample, consisting of a ballistic peak, a narrow diffractive ped ... Cite

Wigner phase space distributions and optical coherence in turbid media

Journal Article Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS · January 1, 1997 Smoothed Wigner phase space contours can be directly measured using a simple heterodyne detection-based optical system employing a He-Ne laser beam split into a 1 mW local oscillator and a 1 mW signal beam, and a spectrum analyzer for real time mean square ... Cite

Optical heterodyne imaging and Wigner phase space distributions.

Journal Article Optics letters · September 1996 We demonstrate that optical heterodyne imaging directly measures smoothed Wigner phase space distributions. This method may be broadly applicable to fundamental studies of light propagation and tomographic imaging. Basic physical properties of Wigner distr ... Full text Cite