Scholars@Duke
Tuan Vo-Dinh

Tuan Vo-Dinh CV

R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical Engineering
Biomedical Engineering
tuan.vodinh@duke.edu
Duke Box 90281, Durham, NC 27708-0281
2589 CIEMAS, Durham, NC 27708
CV

Selected Publications

Plasmonics-enhanced spikey nanorattle-based biosensor for direct SERS detection of mRNA cancer biomarkers.

Journal Article Anal Bioanal Chem · December 2024 We present a plasmonics-enhanced spikey nanorattle-based biosensor for direct surface-enhanced Raman scattering (SERS) detection of mRNA cancer biomarkers. Early detection of cancers such as head and neck squamous cell carcinoma (HNSCC) is critical for imp ... Full text Link to item Cite

Nanoplasmonics biosensors: At the frontiers of biomedical diagnostics

Journal Article TrAC - Trends in Analytical Chemistry · November 1, 2024 This article presents an overview of various nanoplasmonics biosensors and their diverse applications, focusing on recent developments in our laboratory. We describe the versatility and effectiveness of different plasmonics-active platforms, ranging from s ... Full text Cite

Plasmonics nanorod biosensor for in situ intracellular detection of gene expression biomarkers in intact plant systems.

Journal Article Biosensors & bioelectronics · October 2024 The intracellular developmental processes in plants, particularly concerning lignin polymer formation and biomass production are regulated by microRNAs (miRNAs). MiRNAs including miR397b are important for developing efficient and cost-effective biofuels. H ... Full text Cite

Improved solution-based SERS detection of creatinine by inducing hydrogen-bonding interaction for effective analyte capture.

Journal Article Talanta · October 2024 Recently, solution-based surface-enhanced Raman scattering (SERS) detection technique has been widely recognized due to its cost-effectiveness, simplicity, and ease of use. However, solution-based SERS is limited for practical applications mainly because o ... Full text Cite

Plasmonic-Enhanced Colorimetric Lateral Flow Immunoassays Using Bimetallic Silver-Coated Gold Nanostars.

Journal Article ACS applied materials & interfaces · October 2024 The colorimetric lateral flow immunoassay (cLFIA) has gained widespread attention as a point-of-care testing (POCT) technique due to its low cost, short analysis time, portability, and capability of being performed by unskilled operators with minimal requi ... Full text Cite

Nanoengineered plasmonics-enhanced photothermal tags for sensitive detection of cardiac biomarker troponin I using lateral flow immunoassay

Journal Article Chemical Engineering Journal · September 15, 2024 In recent years, photothermal lateral flow immunoassay (LFIA) has gained widespread use as a point-of-care testing (POCT) technique because of its cost-effective portable instrumentation and easy-to-handle procedures. However, developing a highly sensitive ... Full text Cite

A Simple and Sensitive Wearable SERS Sensor Utilizing Plasmonic-Active Gold Nanostars.

Journal Article ACS omega · September 2024 Wearable sweat sensors hold great potential for offering detailed health insights by monitoring various biomarkers present in sweat, such as glucose, lactate, uric acid, and urea, in real time. However, most previously reported sensors, primarily based on ... Full text Cite

Rapid Solution-Based SERS Detection of Pesticides Using Graphene Oxide-Coated Silver-Gold Nanostars

Journal Article ACS Applied Nano Materials · May 24, 2024 This study presents the development of an affordable and straightforward solution-based surface-enhanced Raman scattering (SERS) detection platform, employing plasmonic-active silver-gold nanostars coated with graphene oxide (GO-SGNS). The inclusion of GO ... Full text Cite

Caged gold nanostars: a novel plasmonic nanoplatform with potential theranostic applications.

Journal Article Nanoscale · May 2024 Here, we first introduce caged gold nanostars (C-GNS), a novel hybrid nanoplatform combining the exceptional plasmonic properties of nanostars with the loading capability of hollow-shell structures. We present two synthetic routes used to produce C-GNS par ... Full text Cite

Osmosensor-mediated control of Ca2+ spiking in pollen germination.

Journal Article Nature · May 2024 Higher plants survive terrestrial water deficiency and fluctuation by arresting cellular activities (dehydration) and resuscitating processes (rehydration). However, how plants monitor water availability during rehydration is unknown. Although increases in ... Full text Link to item Cite

Acoustic separation and concentration of exosomes for nucleotide detection: ASCENDx.

Journal Article Sci Adv · March 8, 2024 Efficient isolation and analysis of exosomal biomarkers hold transformative potential in biomedical applications. However, current methods are prone to contamination and require costly consumables, expensive equipment, and skilled personnel. Here, we intro ... Full text Link to item Cite

A simple low-cost flexible plasmonic patch based on spiky gold nanostars for ultra-sensitive SERS sensing.

Journal Article The Analyst · March 2024 Recently, transparent and flexible surface-enhanced Raman scattering (SERS) substrates have received great interest for direct point-of-care detection of analytes on irregular nonplanar surfaces. In this study, we proposed a simple cost-effective strategy ... Full text Cite

Dual-Modal Colorimetric and Surface-Enhanced Raman Scattering (SERS)-Based Lateral Flow Immunoassay for Ultrasensitive Detection of SARS-CoV-2 Using a Plasmonic Gold Nanocrown.

Journal Article Analytical chemistry · March 2024 The 2019 coronavirus disease (COVID-19) outbreak created an unprecedented need for rapid, sensitive, and cost-effective point-of-care diagnostic tests to prevent and mitigate the spread of the SARS-CoV-2 virus. Herein, we demonstrated an advanced lateral f ... Full text Cite

On the Importance of Low-Frequency Signals in Functional and Molecular Photoacoustic Computed Tomography.

Journal Article IEEE transactions on medical imaging · February 2024 In photoacoustic computed tomography (PACT) with short-pulsed laser excitation, wideband acoustic signals are generated in biological tissues with frequencies related to the effective shapes and sizes of the optically absorbing targets. Low-frequency photo ... Full text Cite

Surface-Enhanced Raman Spectroscopy-Based Detection of Micro-RNA Biomarkers for Biomedical Diagnosis Using a Comparative Study of Interpretable Machine Learning Algorithms.

Journal Article Appl Spectrosc · January 2024 Surface-enhanced Raman spectroscopy (SERS) has wide diagnostic applications due to narrow spectral features that allow multiplex analysis. We have previously developed a multiplexed, SERS-based nanosensor for micro-RNA (miRNA) detection called the inverse ... Full text Link to item Cite

Rapid SERS assay for determination of the opioid fentanyl using silver-coated sharply branched gold nanostars.

Journal Article Mikrochimica acta · January 2024 A high-throughput surface-enhanced Raman scattering (SERS)-sensing platform is presented for FNT detection in human urine without any sample preparation. The sensing platform is based on plasmonics-active silver-coated sharply branched gold nanostars (SGNS ... Full text Cite

Multiplex SERS detection of polycyclic aromatic hydrocarbon (PAH) pollutants in water samples using gold nanostars and machine learning analysis.

Journal Article The Analyst · October 2023 Polycyclic aromatic hydrocarbons (PAHs) have attracted a lot of environmental concern because of their carcinogenic and mutagenic properties, and the fact they can easily contaminate natural resources such as drinking water and river water. This study pres ... Full text Cite

Gold Nanostars Obviate Limitations to Laser Interstitial Thermal Therapy (LITT) for the Treatment of Intracranial Tumors.

Journal Article Clin Cancer Res · August 15, 2023 PURPOSE: Laser interstitial thermal therapy (LITT) is an effective minimally invasive treatment option for intracranial tumors. Our group produced plasmonics-active gold nanostars (GNS) designed to preferentially accumulate within intracranial tumors and a ... Full text Open Access Link to item Cite

Xenorecognition and costimulation of porcine endothelium-derived extracellular vesicles in initiating human porcine-specific T cell immune responses.

Journal Article Am J Transplant · July 2023 Porcine vascular endothelial cells (PECs) form a mechanistic centerpiece of xenograft rejection. Here, we determined that resting PECs release swine leukocyte antigen class I (SLA-I) but not swine leukocyte antigen class-II DR (SLA-DR) expressing extracell ... Full text Open Access Link to item Cite

Cascade Amplified Plasmonics Molecular Biosensor for Sensitive Detection of Disease Biomarkers.

Journal Article Biosensors · July 2023 Recent advances in molecular technologies have provided various assay strategies for monitoring biomarkers, such as miRNAs for early detection of various diseases and cancers. However, there is still an urgent unmet need to develop practical and accurate m ... Full text Cite

Ultra-trace SERS detection of cocaine and heroin using bimetallic gold-silver nanostars (BGNS-Ag).

Journal Article Analytica chimica acta · April 2023 A rapid, in-field, and reliable method for the detection of illegal drugs of abuse in biological fluids without any sample pretreatment would potentially be helpful for law enforcement, drug control officials, and public healthcare. In this study, we prese ... Full text Cite

A hybrid plasmonic nanoprobe using polyvinylpyrrolidone-capped bimetallic silver-gold nanostars for highly sensitive and reproducible solution-based SERS sensing.

Journal Article The Analyst · April 2023 Practical solution-based assays using surface-enhanced Raman spectroscopy (SERS) with portable instrumentation are currently of particular interest for rapid, efficient, and low-cost detection of analytes. However, current assays still have limited applica ... Full text Cite

Sharp Branched Gold Nanostar-Based Lateral-Flow Immunoassay for Detection of Yersinia pestis

Journal Article ACS Applied Nano Materials · March 10, 2023 Over the past few decades, colorimetric paper-based lateral flow immunoassay (LFIA) has emerged as a versatile analytical tool for rapid point-of-care detection of infectious diseases with high simplicity and flexibility. The LFIA sensitivity is based on c ... Full text Cite

Plasmonic nanorod probes' journey inside plant cells for in vivo SERS sensing and multimodal imaging.

Journal Article Nanoscale · March 2023 Nanoparticle-based platforms are gaining strong interest in plant biology and bioenergy research to monitor and control biological processes in whole plants. However, in vivo monitoring of biomolecules using nanoparticles inside plant cells remains ... Full text Cite

Solution-Based Ultra-Sensitive Surface-Enhanced Raman Scattering Detection of the Toxin Bacterial Biomarker Pyocyanin in Biological Fluids Using Sharp-Branched Gold Nanostars.

Journal Article Analytical chemistry · February 2023 There is a critical need for sensitive rapid point-of-care detection of bacterial infection biomarkers in complex biological fluids with minimal sample preparation, which can improve early-stage diagnosis and prevent several bacterial infections and fatal ... Full text Cite

miRNA probe integrated biosensor platform using bimetallic nanostars for amplification-free multiplexed detection of circulating colorectal cancer biomarkers in clinical samples.

Journal Article Biosens Bioelectron · January 15, 2023 There is a critical need for sensitive and rapid detection technologies utilizing molecular biotargets such as microRNAs (miRNAs), which regulate gene expression and are a promising class of diagnostic biomarkers for disease detection. Here, we present the ... Full text Link to item Cite

Point-of-care vertical flow immunoassay system for ultra-sensitive multiplex biothreat-agent detection in biological fluids.

Journal Article Biosensors & bioelectronics · January 2023 This paper presents simple, fast, and sensitive detection of multiple biothreat agents by paper-based vertical flow colorimetric sandwich immunoassay for detection of Yersinia pestis (LcrV and F1) and Francisella tularensis (lipopolysaccharide; LPS) antige ... Full text Cite

Voltage-tunable surface-enhanced Raman scattering substrates based on electroactive polymeric membranes containing plasmonic nanoparticles

Journal Article Optics Continuum · December 15, 2022 We describe voltage-controlled surface-enhanced Raman scattering (SERS) substrates in which the SERS-signals can be actively modulated by applying voltage. These SERS-substrates employ a dielectric electroactive polymer (D-EAP) membrane with a pair of elec ... Full text Cite

Machine learning using convolutional neural networks for SERS analysis of biomarkers in medical diagnostics.

Journal Article J Raman Spectrosc · December 2022 Surface-enhanced Raman spectroscopy (SERS) has wide diagnostic applications because of narrow spectral features that allow multiplexed analysis. Machine learning (ML) has been used for non-dye-labeled SERS spectra but has not been applied to SERS dye-label ... Full text Link to item Cite

Optical recognition of constructs using hyperspectral imaging and detection (ORCHID).

Journal Article Scientific reports · December 2022 Challenges to deep sample imaging have necessitated the development of special techniques such as spatially offset optical spectroscopy to collect signals that have travelled through several layers of tissue. However, these techniques provide only spectral ... Full text Cite

Nanoplasmonics Enabling Cancer Diagnostics and Therapy.

Journal Article Cancers · November 2022 In this paper, we highlight several advances our laboratory has developed in the pursuit of cancer diagnostics and therapeutics by integrating plasmonics, photonics, and nanotechnology. We discuss the development and applications of plasmonics-active gold ... Full text Cite

Bimetallic Gold Nanostars Having High Aspect Ratio Spikes for Sensitive Surface-Enhanced Raman Scattering Sensing.

Journal Article ACS applied nano materials · September 2022 There has been increasing interest in evolution of plasmonic nanoplatforms based on noble metal nanoparticles to achieve ultrasensitive detection of trace analyte molecules through solution-based surface-enhanced Raman spectroscopy (SERS). This work presen ... Full text Cite

Optimization of an Antibody Microarray Printing Process Using a Designed Experiment.

Journal Article ACS omega · September 2022 Antibody microarrays have proven useful in immunoassay-based point-of-care diagnostics for infectious diseases. Noncontact piezoelectric inkjet printing has advantages to print antibody microarrays on nitrocellulose substrates for this application due to i ... Full text Cite

Intravital optical imaging for immune cell tracking after photoimmunotherapy with plasmonic gold nanostars.

Journal Article Nanotechnology · August 31, 2022 Bladder cancer has been ranked as one of the most commonly occurring cancers in men and women with approximately half of the diagnoses being the late stage and/or metastatic diseases. We have developed a novel cancer treatment by combining gold nanostar-me ... Full text Link to item Cite

OSCA1 is an osmotic specific sensor: a method to distinguish Ca2+ -mediated osmotic and ionic perception.

Journal Article The New phytologist · August 2022 Genetic mutants defective in stimulus-induced Ca2+ increases have been gradually isolated, allowing the identification of cell-surface sensors/receptors, such as the osmosensor OSCA1. However, determining the Ca2+ -signaling specifici ... Full text Cite

Ultra-high SERS detection of consumable coloring agents using plasmonic gold nanostars with high aspect-ratio spikes.

Journal Article The Analyst · July 2022 Solution-based SERS detection by using a portable Raman instrument has emerged as an important tool due to its simplicity, and flexibility for rapid and on-site screening of analyte molecules. However, this method has several shortcomings, including poor s ... Full text Cite

In vivo SERS monitoring in plants using plasmonic nanoprobes

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2022 Plant biotechnology and biofuel research is critical in addressing increasing global demands for energy. Further understanding of biomass producing associated metabolic pathways in plants can be used to exploit and increase the production of biomass for en ... Full text Cite

Analysis of SERS spectra of plasmonic nanoprobes for multiplexed biomarker detection using machine learning

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2022 Surface-enhanced Raman spectroscopy (SERS) has wide applications in chemical and biosensing as well as imaging. Raman spectra obtained from SERS exhibit characteristic narrow peaks that allow higher degrees of multiplexing than possible with fluorescence i ... Full text Cite

SERS-based molecular sentinel nanoprobes for nucleic acid biomarker detection

Chapter · January 1, 2022 This chapter presents an overview of SERS molecular sentinels for cancer detection. ... Full text Cite

SERS biosensors for point-of-care infectious disease diagnostics

Chapter · January 1, 2022 Timely diagnosis of infectious diseases is critical to healthcare, especially in the time of pandemics. However, most current methods of infectious disease diagnosis are laboratory-based, which typically have a long turn-around time. This causes delays in ... Full text Cite

Flg22-induced Ca2+ increases undergo desensitization and resensitization.

Journal Article Plant, cell & environment · December 2021 The flagellin epitope flg22, a pathogen-associated molecular pattern (PAMP), binds to the receptor-like kinase FLAGELLIN SENSING2 (FLS2), and triggers Ca2+ influx across the plasma membrane (PM). The flg22-induced increases in cytosolic Ca2 ... Full text Cite

Smartphone-Based Device for Colorimetric Detection of MicroRNA Biomarkers Using Nanoparticle-Based Assay.

Journal Article Sensors (Basel, Switzerland) · December 2021 The detection of microRNAs (miRNAs) is emerging as a clinically important tool for the non-invasive detection of a wide variety of diseases ranging from cancers and cardiovascular illnesses to infectious diseases. Over the years, miRNA detection schemes ha ... Full text Cite

Plasmonic Gold Nanostar-Mediated Photothermal Immunotherapy.

Journal Article IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society · September 2021 Cancer is among the leading cause of death around the world, causing close to 10 million deaths each year. Significant efforts have been devoted to developing novel technologies that can detect and treat cancer early and effectively to reduce cancer recurr ... Full text Cite

Plasmonic gold nanostars for synergistic photoimmunotherapy to treat cancer.

Journal Article Nanophotonics · September 2021 Cancer is the second leading cause of death and there is an urgent need to improve cancer management. We have developed an innovative cancer therapy named Synergistic Immuno Photothermal Nanotherapy (SYMPHONY) by combining gold nanostars (GNS)-mediated pho ... Full text Open Access Link to item Cite

Plasmonic nanoplatforms: From surface-enhanced Raman scattering sensing to biomedical applications

Journal Article Journal of Raman Spectroscopy · February 1, 2021 In this work, we present an overview of the development and application of surface-enhanced Raman scattering (SERS) and plasmonic nanoplatforms developed in our laboratory in recent years. We have developed a wide variety of plasmonics-active substrates an ... Full text Cite

Accurate in vivo tumor detection using plasmonic-enhanced shifted-excitation Raman difference spectroscopy (SERDS).

Journal Article Theranostics · January 2021 For the majority of cancer patients, surgery is the primary method of treatment. In these cases, accurately removing the entire tumor without harming surrounding tissue is critical; however, due to the lack of intraoperative imaging techniques, surgeons re ... Full text Cite

Gold Nanostars: A Novel Platform for Developing 211At-Labeled Agents for Targeted Alpha-Particle Therapy.

Journal Article Int J Nanomedicine · 2021 AIM: To develop an innovative 211At nanoplatform with high radiolabeling efficiency and low in vivo deastatination for future targeted alpha-particle therapy (TAT) to treat cancer. METHODS: Star-shaped gold nanoparticles, gold nanostars (GNS), were used as ... Full text Link to item Cite

Plasmonic assay for amplification-free cancer biomarkers detection in clinical tissue samples.

Journal Article Anal Chim Acta · December 1, 2020 Featured Publication Developing countries have seen a rise in cancer incidence and are projected to harbor three-quarters of all cancer-related mortality by 2030. While disproportionally affected by the burden of cancer, these regions are ill-equipped to handle the diagnostic ... Full text Link to item Cite

3D-printed phantoms for characterizing SERS nanoparticle detectability in turbid media.

Journal Article The Analyst · September 2020 Featured Publication Recent advances in plasmonic nanoparticle synthesis have enabled extremely high per-particle surface-enhanced Raman scattering (SERS) efficiencies. This has led to the development of SERS tags for in vivo applications (e.g. tumor targeting and detection), ... Full text Cite

Fibrinogen-Coated Albumin Nanospheres Prevent Thrombocytopenia-Related Bleeding.

Journal Article Radiat Res · August 1, 2020 Featured Publication Thrombocytopenia (TCP) may cause severe and life-threatening bleeding. While this may be prevented by platelet transfusions, transfusions are associated with potential complications, do not always work (platelet refractory) and are not always available. Th ... Full text Link to item Cite

Plasmonic nanobiosensors for detection of microRNA cancer biomarkers in clinical samples.

Journal Article Analyst · July 7, 2020 MicroRNAs (miRNAs) play an important role in the regulation of biological processes and have demonstrated great potential as biomarkers for the early detection of various diseases, including esophageal adenocarcinoma (EAC) and Barrett's esophagus (BE), the ... Full text Link to item Cite

Plasmonic Nanobiosensing: From in situ plant monitoring to cancer diagnostics at the point of care

Journal Article JPhys Photonics · July 7, 2020 Nucleic acid biosensing technologies have the capability to provide valuable information in applications ranging from medical diagnostics to environmental sensing. The unique properties of plasmonic metallic nanoparticles have been used for sensing purpose ... Full text Cite

Direct SERDS sensing of molecular biomarkers in plants under field conditions.

Journal Article Analytical and bioanalytical chemistry · May 2020 Molecular biomarkers such as microRNAs (miRNAs) play important roles in regulating various developmental processes in plants. Understanding these pathways will help bioengineer designing organisms for efficient biomass accumulation. Current methods for RNA ... Full text Cite

Present and Future of Surface-Enhanced Raman Scattering.

Journal Article ACS nano · January 2020 The discovery of the enhancement of Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and analytical techniques. Significant experimental and theoretical effort has been directed toward un ... Full text Cite

Nanostar probes: A golden platform for Synergistic Immuno Photothermal Nanotherapy (SYMPHONY) for the treatment of metastatic cancer

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2020 The discovery of new treatments for cancer is imperative. Recently, we showed in a proof-of-concept study that SYnergistic IMmuno PHOtothermal NanotherapY (SYMPHONY) is a powerful treatment for metastatic bladder cancer and brain tumor in mouse models. Our ... Full text Cite

Applications of plasmonic nanoparticles for in vivo biosensing of plants

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2020 Further understanding of biomass producing associated metabolic pathways in plants can be used to increase the production of biomass. In vivo detection of these markers has proved to be limited due to complex sample preparation required by traditional meth ... Full text Cite

Fiberoptics SERS sensors using plasmonic nanostar probes for detection of molecular biotargets

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2020 Our group has integrated surface-enhanced Raman scattering (SERS) silver coated gold nanostars on an optical fiber. Fiber-based sensors are an in-situ technology that can simultaneously bring the sensor and light to the sample without disturbing the enviro ... Full text Cite

Direct and Label-Free Detection of MicroRNA Cancer Biomarkers using SERS-Based Plasmonic Coupling Interference (PCI) Nanoprobes.

Journal Article The journal of physical chemistry. B · December 2019 MicroRNAs (miRNAs), small noncoding endogenous RNA molecules, are emerging as promising biomarkers for early detection of various diseases and cancers. Practical screening tools and strategies to detect these small molecules are urgently needed to facilita ... Full text Cite

Plasmonic gold nanostar-mediated photothermal immunotherapy for brain tumor ablation and immunologic memory.

Journal Article Immunotherapy · October 2019 Brain tumors present unique therapeutic challenges and they include glioblastoma (GBM) and metastases from cancers of other organs. Current treatment options are limited and include surgical resection, radiation therapy, laser interstitial thermal therapy ... Full text Link to item Cite

Plant cell-surface GIPC sphingolipids sense salt to trigger Ca2+ influx.

Journal Article Nature · August 2019 Salinity is detrimental to plant growth, crop production and food security worldwide. Excess salt triggers increases in cytosolic Ca2+ concentration, which activate Ca2+-binding proteins and upregulate the Na+/H+ ... Full text Cite

Non-invasive sensitive brain tumor detection using dual-modality bioimaging nanoprobe.

Journal Article Nanotechnology · July 5, 2019 Despite decades of efforts, non-invasive sensitive detection of small malignant brain tumors still remains challenging. Here we report a dual-modality 124I-labeled gold nanostar (124I-GNS) probe for sensitive brain tumor imaging with positron emission tomo ... Full text Link to item Cite

Endothelial Cell-Derived Extracellular Vesicles Mitigate Radiation-Induced Hematopoietic Injury.

Journal Article Int J Radiat Oncol Biol Phys · June 1, 2019 PURPOSE: Extracellular vesicles (EVs) are shed vesicles that bear a combination of nucleic acids and proteins. EVs are becoming recognized as a mode of cell-to-cell communication. Because hematopoietic stem cells reside in proximity to endothelial cells (E ... Full text Link to item Cite

Fiber-optrode SERS probes using plasmonic silver-coated gold nanostars

Journal Article Sensors and Actuators, B: Chemical · May 15, 2019 Surface-enhanced Raman scattering (SERS)-based molecular sensing has emerged as a powerful tool in a wide variety of fields ranging from environmental monitoring, biomedical diagnostics to security applications. As versatile SERS probes, fiber-optrodes (i. ... Full text Cite

Inverse Molecular Sentinel-Integrated Fiberoptic Sensor for Direct and in Situ Detection of miRNA Targets.

Journal Article Analytical chemistry · May 2019 Molecular advances have been made in analysis systems for a wide variety of applications ranging from biodiagnostics, biosafety, bioengineering, and biofuel research applications. There are, however, limited practical tools necessary for in situ and accura ... Full text Cite

SERS in Plain Sight: A Polarization Modulation Method for Signal Extraction.

Journal Article Analytical chemistry · March 2019 Surface-enhanced Raman spectroscopy (SERS) is a powerful analytical spectroscopy offering advantages ranging from "vibrational fingerprints" to multiplexed detection. However, the use of this technique in real-world applications has been limited due to dif ... Full text Cite

Plasmonic Nanoprobes for in Vivo Multimodal Sensing and Bioimaging of MicroRNA within Plants.

Journal Article ACS applied materials & interfaces · February 2019 Monitoring gene expression within whole plants is critical for many applications ranging from plant biology to agricultural biotechnology and biofuel development; however, no method currently exists for in vivo monitoring of genomic targets in plant system ... Full text Cite

Synthesis and functionalization of gold nanostars for singlet oxygen production

Journal Article Journal of Industrial and Engineering Chemistry · January 25, 2019 Gold nanostars (GNSs), were synthesized and coated with N-(3-aminopropyl) methacrylamide hydrochloride (PA) in order to functionalize their surfaces by amine groups as well as to investigate the production of singlet oxygen (1O2). The singlet oxygen produc ... Full text Cite

A nanophotonic-based assay for point-of-care medical diagnostics of malaria in low and middle income countries

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2019 Direct detection of genetic biomarkers in tissue and body fluids without complex target extraction and amplification processes can revolutionize nucleic acid-based diagnostics by enabling the use of this technology at the point-of-care. The development of ... Full text Cite

Direct detection of nanostar probes through a monkey skull using inverse surface-enhanced spatially offset Raman spectroscopy (SESORS)

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2019 While the use of nanomaterials in medical diagnostics has received increasing interest, in vivo detection of nanoparticles using optical techniques is still a challenge. Among other techniques, surface-enhanced Raman scattering (SERS)-labeled nanoparticles ... Full text Cite

Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars.

Journal Article Int J Nanomedicine · 2019 Aim: To quantitatively and sensitively investigate the biodistribution of immune cells after systemic administration. Methods: Immune cells were loaded with plasmonic gold nanostars (GNS) tracking probes. Inductively coupled plasma mass spectrometry (ICP-M ... Full text Link to item Cite

Implantable "smart tattoo" SERS nanosensors for in vivo detection of nucleic acid biotargets in a large animal model

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2019 Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo molecular detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid target ... Full text Cite

Application of plasmonic nanoprobes for SERS sensing and imaging of biotargets in plant systems

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2019 The knowledge over gene expression dynamics and location in plants is crucial for applications ranging from basic biological research to agricultural biotechnology (e.g., biofuel development). However, current methods cannot provide in vivo dynamic detecti ... Full text Cite

In vivo nucleic acid detection and imaging within whole plants using plasmonic nanosensors

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2019 The ability to monitor gene expression within living plants is of importance in many applications ranging from plant biology research to biofuel development; however, no method currently exists without requiring sample extraction. Herein, we report a multi ... Full text Cite

Surface-enhanced spatially offset Raman spectroscopy (SESORS) for subsurface detection of nanostar probes

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2019 Early detection of molecular targets can greatly impact the clinical diagnosis and outcome of many diseases such as cancer. Methods utilizing optical detection systems and Surface-Enhanced Raman Scattering (SERS)-labeled nanoparticles provide a way of sele ... Full text Cite

In vivo detection of microRNA within plants using plasmonic nanosensors

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2019 Gene expression monitoring within whole plants is critical for many applications ranging from plant biology to biofuel development. Herein, we report a unique multimodal method for in vivo imaging and biosensing of nucleic acid biotargets, specifically mic ... Full text Cite

Inverse molecular sentinel-integrated fiber sensor for direct detection of miRNA targets

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2019 Molecular analysis has revolutionized many applications, including bio-safety, bio-engineering and biofuel research; however, there are limited practical tools for in situ detection during field work. New technology is needed to translate molecular advance ... Full text Cite

Direct detection of cancer biomarkers using plasmonics-based Inverse Molecular Sentinel (iMS) nanobiosensors

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2019 Development of simple techniques for detection of nucleic acids is essential for clinical diagnostics. MicroRNA (miRNA) have been identified as potential biomarkers in early detection of various diseases, including esophageal adenocarcinoma (EAC) and Barre ... Full text Cite

Shifted-excitation Raman difference spectroscopy for the detection of SERS-encoded gold nanostar probes

Journal Article Journal of Raman Spectroscopy · December 1, 2018 In vivo applications of surface-enhanced Raman spectroscopy (SERS) have limited sensitivity due to the intense background signal from real-life complex samples such as plants and tissues. To overcome this issue, this work investigates the combination of sh ... Full text Cite

Tailoring the Core-Satellite Nanoassembly Architectures by Tuning Internanoparticle Electrostatic Interactions.

Journal Article Langmuir : the ACS journal of surfaces and colloids · December 2018 The use of plasmonic nanoplatforms has received increasing interest in a wide variety of fields ranging from theranostics to environmental sensing to plant biology. In particular, the development of plasmonic nanoparticles into ordered nanoclusters has bee ... Full text Cite

Correction: Determination of ferric ions using surface-enhanced Raman scattering based on desferrioxamine-functionalized silver nanoparticles.

Journal Article Chemical communications (Cambridge, England) · September 2018 Correction for 'Determination of ferric ions using surface-enhanced Raman scattering based on desferrioxamine-functionalized silver nanoparticles' by Fei Yan et al., Chem. Commun., 2013, 49, 7962-7964. ... Full text Cite

Gold nanoparticles-mediated photothermal therapy and immunotherapy.

Journal Article Immunotherapy · September 2018 Cancer has been a significant threat to human health with more than eight million deaths each year in the world. Therefore, there is a significant need for novel technologies to effectively treat cancer and ultimately reduce cancer recurrences, treatment c ... Full text Cite

Inverse surface-enhanced spatially offset Raman spectroscopy (SESORS) through a monkey skull

Journal Article Journal of Raman Spectroscopy · September 1, 2018 The use of nanoparticles in nanomedicine has received increasing interest. However, in vivo detection of nanoparticles using optical techniques is still a formidable challenge. Detecting surface-enhanced Raman scattering (SERS)-labeled nanoparticles throug ... Full text Cite

Author Correction: Rapid Nanophotonics Assay for Head and Neck Cancer Diagnosis.

Journal Article Sci Rep · August 23, 2018 A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ... Full text Link to item Cite

Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid targets in a large animal model

Journal Article Nano Research · August 1, 2018 Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid targets using a ... Full text Open Access Cite

Rapid Nanophotonics Assay for Head and Neck Cancer Diagnosis.

Journal Article Sci Rep · July 30, 2018 Efficient and timely diagnosis of head and neck squamous cell carcinoma (HNSCC) is a critical challenge, particularly in low and middle income countries. These regions, which are expected to witness a drastic increase in HNSCC rates, are ill-prepared to ha ... Full text Link to item Cite

Direct Detection of Unamplified Pathogen RNA in Blood Lysate using an Integrated Lab-in-a-Stick Device and Ultrabright SERS Nanorattles.

Journal Article Sci Rep · March 6, 2018 Direct detection of genetic biomarkers in body fluid lysate without target amplification will revolutionize nucleic acid-based diagnostics. However, the low concentration of target sequences makes this goal challenging. We report a method for direct detect ... Full text Link to item Cite

Spectroscopic chemical sensing and imaging: From plants to animals and humans

Journal Article Chemosensors · March 1, 2018 Chemical sensing and imaging technologies are of great importance in medical diagnostics and environmental sensing due to their ability to detect and localize chemical targets and provide valuable information in real-time. Biophotonic techniques are the mo ... Full text Cite

Manipulation of the Geometry and Modulation of the Optical Response of Surfactant-Free Gold Nanostars: A Systematic Bottom-Up Synthesis.

Journal Article ACS omega · February 2018 Among plasmonic nanoparticles, surfactant-free branched gold nanoparticles have exhibited exceptional properties as a nanoplatform for a wide variety of applications ranging from surface-enhanced Raman scattering sensing and imaging applications to phototh ... Full text Cite

Creation of an optically tunable, solid tissue phantom for use in cancer detection

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2018 An optically tunable, solid tissue phantom was developed in order to aid in the verification and validation of non-destructive cancer detection technologies based on fluorescence spectroscopy. The solid tissue phantom contained agarose, hemoglobin, Intrali ... Full text Cite

Synergistic immuno photothermal nanotherapy (SYMPHONY) to treat metastatic cancers and induce anti-cancer vaccine effect

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2018 Biocompatible gold nanostars (GNS) with tip-enhanced electromagnetic and optical properties have been developed and applied for multifunctional cancer diagnostics and therapy (theranostics). Their multiple sharp branches acting like "lightning rods" can co ... Full text Cite

Surface-enhanced spatially offset Raman spectroscopy (SESORS) for biomedical applications

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2018 Detection of surface-enhanced Raman scattering (SERS) tagged nanoparticles in-vivo is critical for its potential application in cancer diagnostics, inflammation monitoring, and glucose quantification. However, traditional optical methods are typically limi ... Full text Cite

Squamous Cell Carcinoma DNA Detection Using Ultrabright SERS Nanorattles and Magnetic Beads for Head and Neck Cancer Molecular Diagnostics.

Journal Article Anal Methods · October 7, 2017 A rise in head and neck cancers in low and middle countries over recent years has prompted the need for low-cost, resource-efficient diagnostic technologies. Standard diagnosis with histopathology is often not feasible due to the low number of trained path ... Full text Link to item Cite

Synergistic Immuno Photothermal Nanotherapy (SYMPHONY) for the Treatment of Unresectable and Metastatic Cancers.

Journal Article Sci Rep · August 17, 2017 Metastatic spread is the mechanism in more than 90 percent of cancer deaths and current therapeutic options, such as systemic chemotherapy, are often ineffective. Here we provide a proof of principle for a novel two-pronged modality referred to as Synergis ... Full text Open Access Link to item Cite

Human Adipose-Derived Stem Cells Labeled with Plasmonic Gold Nanostars for Cellular Tracking and Photothermal Cancer Cell Ablation.

Journal Article Plast Reconstr Surg · April 2017 BACKGROUND: Gold nanostars are unique nanoplatforms that can be imaged in real time and transform light energy into heat to ablate cells. Adipose-derived stem cells migrate toward tumor niches in response to chemokines. The ability of adipose-derived stem ... Full text Link to item Cite

Plasmonic SERS nanochips and nanoprobes for medical diagnostics and bio-energy applications

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2017 The development of rapid, easy-to-use, cost-effective, high accuracy, and high sensitive DNA detection methods for molecular diagnostics has been receiving increasing interest. Over the last five years, our laboratory has developed several chip-based DNA d ... Full text Cite

SERS-based inverse molecular sentinel (iMS) nanoprobes for multiplexed detection of microRNA cancer biomarkers in biological samples

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2017 The development of sensitive and selective biosensing techniques is of great interest for clinical diagnostics. Here, we describe the development and application of a surface enhanced Raman scattering (SERS) sensing technology, referred to as "inverse Mole ... Full text Cite

Nanosensors for nucleic acid targets detection using SERS

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2017 Single cell analysis can aid the study of molecular events responsible for cellular functions and unveil their connections to the biological functions of an organism. Biosensors based on surface enhanced Raman spectroscopy (SERS) can be used to this end an ... Full text Cite

Photothermal ablation of inflammatory breast cancer tumor emboli using plasmonic gold nanostars.

Journal Article Int J Nanomedicine · 2017 Inflammatory breast cancer (IBC) is rare, but it is the most aggressive subtype of breast cancer. IBC has a unique presentation of diffuse tumor cell clusters called tumor emboli in the dermis of the chest wall that block lymph vessels causing a painful, e ... Full text Link to item Cite

Plasmonic nanochip for SERS chemical and biomedical sensing

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2017 The development of rapid, easy-to-use and highly sensitive DNA detection methods has received increasing interest for medical diagnostics and research purposes. Our laboratory has developed several chip-based DNA biosensors including molecular sentinel-on- ... Full text Cite

Preface

Book · January 1, 2017 Full text Cite

Monitoring apoptosis and anticancer drug activity in single cells using nanosensors

Chapter · January 1, 2017 One of the key challenges in biology and medicine has been molecular analysis of single living cells aimed at providing an understanding of the underlying complex mechanisms in the basic, structural, and functional unit of life. This is important in unders ... Full text Cite

Medical applications of photonics

Chapter · January 1, 2017 Over the past two decades, optic and photonic technologies have become exceedingly ubiquitous in the key enabling devices that are central to many aspects of everyday life. The importance of these paradigm-shifting technologies has so been recognized and a ... Full text Cite

Improvement in Electrotransfection of Cells Using Carbon-Based Electrodes.

Journal Article Cellular and molecular bioengineering · December 2016 Electrotransfection has been widely used as a versatile, non-viral method for gene delivery. However, electrotransfection efficiency (eTE) is still low and unstable, compared to viral methods. To understand potential mechanisms of the unstable eTE, we inve ... Full text Cite

Folate Receptor-Targeted Theranostic Nanoconstruct for Surface-Enhanced Raman Scattering Imaging and Photodynamic Therapy.

Journal Article ACS omega · October 2016 We report the synthesis of a folate receptor (FR)-targeted theranostic nanocomposite for surface-enhanced Raman scattering (SERS) imaging and photodynamic therapy (PDT). FR-specific SERS detection and PDT are demonstrated in vitro using two FR-positive can ... Full text Cite

Multiplexed Detection of MicroRNA Biomarkers Using SERS-Based Inverse Molecular Sentinel (iMS) Nanoprobes.

Journal Article The journal of physical chemistry. C, Nanomaterials and interfaces · September 2016 MicroRNAs (miRNAs) have demonstrated great promise as a novel class of biomarkers for early detection of various cancers, including breast cancer. However, due to technical difficulties in detecting these small molecules, miRNAs have not been adopted into ... Full text Cite

Sensitive DNA detection and SNP discrimination using ultrabright SERS nanorattles and magnetic beads for malaria diagnostics.

Journal Article Biosens Bioelectron · July 15, 2016 One of the major obstacles to implement nucleic acid-based molecular diagnostics at the point-of-care (POC) and in resource-limited settings is the lack of sensitive and practical DNA detection methods that can be seamlessly integrated into portable platfo ... Full text Link to item Cite

Reversible Gating of Plasmonic Coupling for Optical Signal Amplification.

Journal Article ACS applied materials & interfaces · July 2016 Amplification of optical signals is useful for a wide variety of applications, ranging from data signal transmission to chemical sensing and biomedical diagnostics. One such application in chemical sensing is surface-enhanced Raman scattering (SERS), an im ... Full text Cite

“Dry-state” surface-enhanced Raman scattering (SERS): toward non-destructive analysis of dyes on textile fibers

Journal Article Applied Physics A: Materials Science and Processing · July 1, 2016 In the present work, we report the proof of concept of the possibility to identify natural dyes on textiles using surface-enhanced Raman scattering (SERS) detection by means of a simple “dry-state” SERS approach, i.e., exploiting the interactions between a ... Full text Cite

Plasmon-Resonant Gold Nanostars with Variable Size as Contrast Agents for Imaging Applications

Journal Article IEEE Journal of Selected Topics in Quantum Electronics · May 1, 2016 Plasmon-resonant nanostars (NSts) have recently found applications in biophotonics due to their unique optical and chemical characteristics, showing comparable or superior properties than other anisotropic plasmon-resonant nanoparticles. In this paper, we ... Full text Cite

Tunable and amplified Raman gold nanoprobes for effective tracking (TARGET): in vivo sensing and imaging.

Journal Article Nanoscale · April 28, 2016 We describe the development of a highly tunable, physiologically stable, and ultra-bright Raman probe, named as TARGET (Tunable and Amplified Raman Gold Nanoprobes for Effective Tracking), for in vitro and in vivo surface-enhanced Raman scattering (SERS) a ... Full text Link to item Cite

Tracking mesenchymal stromal cells using an ultra-bright TAT-functionalized plasmonic-active nanoplatform.

Journal Article J Biophotonics · April 2016 High-resolution tracking of stem cells remains a challenging task. An ultra-bright contrast agent with extended intracellular retention is suitable for in vivo high-resolution tracking of stem cells following the implantation. Here, a plasmonic-active nano ... Full text Link to item Cite

Optical Fano resonances in a nonconcentric nanoshell.

Journal Article Applied optics · April 2016 The interaction of light with a metal nanoshell with an off-center core generates multipoles of all orders. We show here that the matrix elements used to compute the multipole expansion coefficients can be derived analytically and, with this result, we can ... Full text Cite

Plasmonic SERS biosensing nanochips for DNA detection.

Journal Article Analytical and bioanalytical chemistry · March 2016 The development of rapid, cost-effective DNA detection methods for molecular diagnostics at the point-of-care (POC) has been receiving increasing interest. This article reviews several DNA detection techniques based on plasmonic-active nanochip platforms d ... Full text Cite

Photothermal effects of plasmonic metal nanoparticles in a fluid

Journal Article Journal of Applied Physics · February 28, 2016 There is a strong interest in the use of plasmonic metal nanoparticles in medical applications involving photothermal therapy. In this study, the problem of calculating the temperature elevation of a fluid arising from the absorption of light by a suspensi ... Full text Cite

Plasmonic fano resonance sensing system using gold nanosphere and J-aggregates

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2016 Demonstrated herein is a simple method for the induction of J-aggregate formation in a colloidal solution of gold nanoparticles through the use of pseudoisocyanine (PIC) and polyvinyl sulfate. The plasmon-exciton coupling of the nanoparticle J-aggregate co ... Full text Cite

In vivo detection of SERS-encoded plasmonic nanostars in human skin grafts and live animal models.

Journal Article Anal Bioanal Chem · November 2015 Surface-enhanced Raman scattering (SERS)-active plasmonic nanomaterials have become a promising agent for molecular imaging and multiplex detection. Among the wide variety of plasmonics-active nanoparticles, gold nanostars offer unique plasmon properties t ... Full text Link to item Cite

Fano resonance in a gold nanosphere with a J-aggregate coating.

Journal Article Physical chemistry chemical physics : PCCP · October 2015 We present a facile method to induce J-aggregate formation on gold nanospheres in colloidal solution using polyvinylsulfate. The nanoparticle J-aggregate complex results in an absorption spectrum with a split lineshape due to plasmon-exciton coupling, i.e. ... Full text Cite

Time-Resolved Synchronous Fluorescence for Biomedical Diagnosis.

Journal Article Sensors (Basel) · August 31, 2015 This article presents our most recent advances in synchronous fluorescence (SF) methodology for biomedical diagnostics. The SF method is characterized by simultaneously scanning both the excitation and emission wavelengths while keeping a constant waveleng ... Full text Open Access Link to item Cite

Silver embedded nanostars for SERS with internal reference (SENSIR)

Journal Article Journal of Materials Chemistry C · July 28, 2015 We report the synthesis of a unique hybrid nanoplatform consisting of silver-embedded gold nanostars that entraps a reference dye between the gold and silver surfaces, providing an internal reference signal for quantitative surface-enhanced Raman scatterin ... Full text Cite

Plasmonics-based SERS nanobiosensor for homogeneous nucleic acid detection.

Journal Article Nanomedicine : nanotechnology, biology, and medicine · May 2015 Developing a simple and efficient nucleic acid detection technology is essential for clinical diagnostics. Here, we describe a new conceptually simple and selective "turn on" plasmonics-based nanobiosensor, which integrates non-enzymatic DNA strand-displac ... Full text Cite

Inherently stealthy and highly tumor-selective gold nanoraspberries for photothermal cancer therapy.

Journal Article Scientific reports · May 2015 Owing to their unique optical properties such as large absorption and scattering cross section and large enhancement of electromagnetic field at the surface, plasmonic nanostructures have received extensive attention as a highly promising class of material ... Full text Cite

Plasmonic gold nanostars for multi-modality sensing and diagnostics.

Journal Article Sensors (Basel, Switzerland) · February 2015 Gold nanostars (AuNSs) are unique systems that can provide a novel multifunctional nanoplatform for molecular sensing and diagnostics. The plasmonic absorption band of AuNSs can be tuned to the near infrared spectral range, often referred to as the "tissue ... Full text Cite

SERS nanosensors and nanoreporters: golden opportunities in biomedical applications.

Journal Article Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · January 2015 This article provides an overview of recent developments and applications of surface-enhanced Raman scattering (SERS) nanosensors and nanoreporters in our laboratory for use in biochemical monitoring, medical diagnostics, and therapy. The design and fabric ... Full text Cite

A Plasmonic Gold Nanostar Theranostic Probe for In Vivo Tumor Imaging and Photothermal Therapy.

Journal Article Theranostics · 2015 Nanomedicine has attracted increasing attention in recent years, because it offers great promise to provide personalized diagnostics and therapy with improved treatment efficacy and specificity. In this study, we developed a gold nanostar (GNS) probe for m ... Full text Open Access Link to item Cite

Multiplex DNA biosensor for viral infection diagnosis using SERS molecular sentinel-on-chip

Conference IFMBE Proceedings · January 1, 2015 The development of sensitive and selective techniques for multiplex detection of DNA biomarkers is paramount for clinical diagnosis. Various multiplex DNA detection techniques have been reported. However, most of these techniques require multiple incubatio ... Full text Cite

Multifunctional gold nanostars for molecular imaging and cancer therapy.

Journal Article Frontiers in chemistry · January 2015 Plasmonics-active gold nanoparticles offer excellent potential in molecular imaging and cancer therapy. Among them, gold nanostars (AuNS) exhibit cross-platform flexibility as multimodal contrast agents for macroscopic X-ray computer tomography (CT), magne ... Full text Cite

Plasmonics-Active gold nanostars for chemical and biological sensing using SERS detection

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2015 Surface-enhanced Raman scattering (SERS) provides a non-destructive sensing method with high sensitivity and multiplex detection capability since SERS takes advantage of high enhancement from surface plasmon resonance and unique â€fingerprint†spectral si ... Full text Cite

DNA bioassay-on-chip using SERS detection for dengue diagnosis.

Journal Article Analyst · November 21, 2014 A novel DNA bioassay-on-chip using surface-enhanced Raman scattering (SERS) on a bimetallic nanowave chip is presented. In this bioassay, SERS signals were measured after a single reaction on the chip's surface without any washing step, making it simple-to ... Full text Link to item Cite

Direct analysis of traditional Chinese medicines using Surface-Enhanced Raman Scattering (SERS).

Journal Article Drug testing and analysis · October 2014 Surface-Enhanced Raman Scattering (SERS) spectrometry provides an excellent tool to characterize chemical constituents in Traditional Chinese Medicines (TCMs) without requiring separation and extraction procedures. This study involved the use of SERS to an ... Full text Cite

Biosensors for medical applications

Chapter · July 29, 2014 Cite

Preface

Book · July 29, 2014 Cite

Optical properties of tissue

Chapter · July 29, 2014 Cite

Biomedical Photonics Handbook

Book · July 29, 2014 Shaped by Quantum Theory, Technology, and the Genomics Revolution The integration of photonics, electronics, biomaterials, and nanotechnology holds great promise for the future of medicine. This topic has recently experienced an explosive growth due to the ... Full text Cite

Preface

Book · July 29, 2014 Cite

Preface

Book · July 29, 2014 Cite

Basic instrumentation in photonics.

Chapter · July 29, 2014 Cite

Multiplex detection of disease biomarkers using SERS molecular sentinel-on-chip.

Journal Article Anal Bioanal Chem · May 2014 Developing techniques for multiplex detection of disease biomarkers is important for clinical diagnosis. In this work, we have demonstrated for the first time the feasibility of multiplex detection of genetic disease biomarkers using the surface-enhanced R ... Full text Link to item Cite

Plasmonics-enhanced and optically modulated delivery of gold nanostars into brain tumor.

Journal Article Nanoscale · April 21, 2014 Plasmonics-active gold nanostars exhibiting strong imaging contrast and efficient photothermal transduction were synthesized for a novel pulsed laser-modulated plasmonics-enhanced brain tumor microvascular permeabilization. We demonstrate a selective, opti ... Full text Link to item Cite

Development of Hybrid Silver-Coated Gold Nanostars for Nonaggregated Surface-Enhanced Raman Scattering.

Journal Article The journal of physical chemistry. C, Nanomaterials and interfaces · February 2014 In the ongoing search for ever-brighter surface-enhanced Raman scattering (SERS) nanoprobes, gold nanostars (AuNSs) have emerged as one of the best geometries for producing SERS in a nonaggregated state. Despite their high enhancement factor, optical extin ... Full text Cite

Enhanced SPR Sensitivity with Nano-Micro-Ribbon Grating-an Exhaustive Simulation Mapping

Journal Article Plasmonics · February 1, 2014 In this study, we theoretically investigate the sensing potential of 2D nano- and micro-ribbon grating structuration on the surface of Kretschmann-based surface plasmon resonance (SPR) biosensors when they are employed for detection of biomolecular binding ... Full text Cite

Optically enhanced blood-brain-barrier crossing of plasmonic-active nanoparticles in preclinical brain tumor animal models

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2014 Nanotechnology provides tremendous biomedical opportunities for cancer diagnosis, imaging, and therapy. In contrast to conventional chemotherapeutic agents where their actual target delivery cannot be easily imaged, integrating imaging and therapeutic prop ... Full text Cite

Plasmonic gold nanostar for biomedical sensing

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2014 Cancer has become one of most significant death reasons and causes approximately 7.9 million human deaths worldwide each year. The challenge to detect cancer at an early stage makes cancer-related biomarkers sensing attract more and more research interest ... Full text Cite

Micovascular integration into porous polyHEMA scaffold

Chapter · January 1, 2014 Surface-enhanced Raman scattering (SERS) spectroscopy can be a useful tool in regard to disease diagnosis and prevention. Advantage of SERS over conventional Raman spectroscopy is its significantly increased signal (up to factor of 106-108) which allows de ... Full text Cite

Introduction to the issue on nanobiophotonics

Journal Article IEEE Journal on Selected Topics in Quantum Electronics · January 1, 2014 Full text Cite

Preface

Chapter · January 1, 2014 Full text Cite

Preface

Chapter · January 1, 2014 Full text Cite

Preface

Chapter · January 1, 2014 Full text Cite

Introduction

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2014 Cite

Quintuple-modality (SERS-MRI-CT-TPL-PTT) plasmonic nanoprobe for theranostics.

Journal Article Nanoscale · December 21, 2013 A unique quintuple-modality theranostic nanoprobe (QMT) is developed with gold nanostars for surface-enhanced Raman scattering (SERS), magnetic resonance imaging (MRI), computed tomography (CT), two-photon luminescence (TPL) imaging and photothermal therap ... Full text Link to item Cite

Plasmonic nanoprobes: from chemical sensing to medical diagnostics and therapy.

Journal Article Nanoscale · November 2013 This article provides an overview of the development and applications of plasmonics-active nanoprobes in our laboratory for chemical sensing, medical diagnostics and therapy. Molecular Sentinel nanoprobes provide a unique tool for DNA/RNA biomarker detecti ... Full text Cite

Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics.

Journal Article Anal Chim Acta · July 5, 2013 In this paper, we describe a surface-enhanced Raman scattering (SERS)-based detection approach, referred to as "molecular sentinel" (MS) plasmonic nanoprobes, to detect an RNA target related to viral infection. The MS method is essentially a label-free tec ... Full text Link to item Cite

Plasmonic nanoprobes for intracellular sensing and imaging.

Journal Article Analytical and bioanalytical chemistry · July 2013 Recent advances in integrating nanotechnology and optical microscopy offer great potential in intracellular applications with improved molecular information and higher resolution. Continuous efforts in designing nanoparticles with strong and tunable plasmo ... Full text Cite

Label-free DNA biosensor based on SERS Molecular Sentinel on Nanowave chip.

Journal Article Analytical chemistry · July 2013 Development of a rapid, cost-effective, label-free biosensor for DNA detection is important for many applications in clinical diagnosis, homeland defense, and environment monitoring. A unique label-free DNA biosensor based on Molecular Sentinel (MS) immobi ... Full text Cite

Cell-penetrating peptide enhanced intracellular Raman imaging and photodynamic therapy.

Journal Article Molecular pharmaceutics · June 2013 We present the application of a theranostic system combining Raman imaging and the photodynamic therapy (PDT) effect. The theranostic nanoplatform was created by loading the photosensitizer, protoporphyrin IX, onto a Raman-labeled gold nanostar. A cell-pen ... Full text Cite

Progress in Biomedical Optics and Imaging - Proceedings of SPIE: Introduction

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · May 22, 2013 This PDF file contains the front matter associated with SPIE Proceedings Volume 8571, including the Title Page, Copyright Information, Table of Contents, and the Conference Committee listing. © 2013 Copyright SPIE. ... Full text Cite

Progress in Biomedical Optics and Imaging - Proceedings of SPIE: Introduction

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · May 22, 2013 This PDF file contains the front matter associated with SPIE Proceedings Volume 8572, including the Title Page, Copyright Information, Table of Contents, and the Conference Committee listing. © 2013 Copyright SPIE. ... Full text Cite

Molecular sentinel-on-chip for SERS-based biosensing.

Journal Article Physical chemistry chemical physics : PCCP · April 2013 The development of DNA detection techniques on large-area plasmonics-active platforms is critical for many medical applications such as high-throughput screening, medical diagnosis and systems biology research. Here, we report for the first time a unique " ... Full text Cite

Spectroscopic and vibrational analysis of the methoxypsoralen system: A comparative experimental and theoretical study

Journal Article Journal of Molecular Structure · March 13, 2013 Raman spectra measurements and density functional theory (DFT) calculations were performed to investigate three psoralens: 5-amino-8-methoxypsoralen (5-A-8-MOP), 5-methoxypsoralen (5-MOP) and 8-methoxypsoralen (8-MOP) with the aim of differentiating these ... Full text Cite

Spectral Characterization and Intracellular Detection of Surface-Enhanced Raman Scattering (SERS)-Encoded Plasmonic Gold Nanostars.

Journal Article Journal of Raman spectroscopy : JRS · February 2013 Plasmonic gold nanostars offer a new platform for Surface-Enhanced Raman Scattering (SERS). However, due to the presence of organic surfactant on the nanoparticles, SERS characterization and application of nanostar ensembles in solution have been challengi ... Full text Cite

Quantitative surface-enhanced resonant Raman scattering multiplexing of biocompatible gold nanostars for in vitro and ex vivo detection.

Journal Article Analytical chemistry · January 2013 Surface-enhanced Raman scattering (SERS)-active plasmonic nanomaterials have become a promising agent for molecular imaging and multiplex detection. To produce strong SERS intensity while retaining the nonaggregated state and biocompatibility needed for bi ... Full text Cite

PH-sensing nanostar probe using surface-enhanced Raman scattering (SERS): Theoretical and experimental studies

Journal Article Journal of Raman Spectroscopy · January 1, 2013 Local pH environment has been considered to be a potential biomarker for tumor diagnosis because solid tumors contain highly acidic environments. A pH-sensing nanoprobe based on surface-enhanced Raman scattering (SERS) using nanostars under near-infrared e ... Full text Cite

Angle-dependent resonance of localized and propagating surface plasmons in microhole arrays for enhanced biosensing.

Journal Article Analytical and bioanalytical chemistry · December 2012 The presence of microhole arrays in thin Au films is suited for the excitation of localized and propagating surface plasmon (SP) modes. Conditions can be established to excite a resonance between the localized and propagating SP modes, which further enhanc ... Full text Cite

Optical nanosensors and nanoprobes: From single-cell exploration to medical diagnostics

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 2012 This lecture presents an overview of recent advances in the development of optical nanobiosensor and nanoprobe technology at the nexus of engineering, biology, medicine and nanotechnology. The presentation describes two areas of research related to the dev ... Full text Cite

In vivo particle tracking and photothermal ablation using plasmon-resonant gold nanostars.

Journal Article Nanomedicine · November 2012 UNLABELLED: Gold nanostars offer unique plasmon properties that efficiently transduce photon energy into heat for photothermal therapy. Nanostars, with their small core size and multiple long thin branches, exhibit high absorption cross-sections that are t ... Full text Link to item Cite

Bimodal behavior and isobestic transition pathway in surface plasmon resonance sensing.

Journal Article Optics express · October 2012 In traditional interpretation of surface plasmon resonance (SPR) sensing and imaging data, total surface coverage of adsorbed or deposited chemical and biological molecules is generally assumed. This homogenous assumption leads to the modeling of monomodal ... Full text Cite

Guest editorial introduction to the issue on biophotonics - Part 2

Journal Article IEEE Journal on Selected Topics in Quantum Electronics · July 9, 2012 Full text Cite

TAT peptide-functionalized gold nanostars: enhanced intracellular delivery and efficient NIR photothermal therapy using ultralow irradiance.

Journal Article Journal of the American Chemical Society · July 2012 Gold nanoparticles have great potential in plasmonic photothermal therapy (photothermolysis), but their intracellular delivery and photothermolysis efficiency have yet to be optimized. We show that TAT-peptide-functionalized gold nanostars (NS) enter cells ... Full text Cite

The effect of reactive atypia/inflammation on the laser-induced fluorescence diagnosis of non-dysplastic Barrett's esophagus.

Journal Article Lasers in surgery and medicine · July 2012 Background and objectivesDifferential Normalized Fluorescence (DNF) technique has been used to distinguish high-grade dysplasia from non-dysplastic Barrett's esophagus. This technology may assist gastroenterologists in targeting biopsies, reducing ... Full text Cite

Introduction to the issue on biophotonics-part 1

Journal Article IEEE Journal on Selected Topics in Quantum Electronics · June 20, 2012 Full text Cite

Plasmonic coupling interference: A new approach for cancer diagnostics using SERS detection

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · June 12, 2012 We have developed a novel label-free approach referred to as plasmonic coupling interference (PCI) nanoprobes for nucleic acid detection using surface-enhanced Raman scattering (SERS). To induce a strong plasmonic coupling effect and SERS signal, a nano-ne ... Full text Cite

Imaging a spatially confined photoacoustic source defined by a distribution of plasmonic nanoparticles

Journal Article Journal of Applied Physics · May 1, 2012 This paper describes the use of plasmonic nanoparticles in photoacoustic imaging. When acoustic waves are generated by thermoacoustic expansion in the fluid medium surrounding a distribution of these particles and the acoustic signals are recorded over a p ... Full text Cite

Plasmonic "Nanowave" Substrates for SERS: Fabrication and Numerical Analysis.

Journal Article The journal of physical chemistry. C, Nanomaterials and interfaces · April 2012 The "Nanowave" substrate, comprising a close-packed array of nanospheres onto which a thin metal shell of silver or gold is deposited, was first fabricated in our laboratory in 1984 and used as a surface-enhanced Raman scattering (SERS)-active substrate fo ... Full text Cite

Assessing the Location of Surface Plasmons Over Nanotriangle and Nanohole Arrays of Different Size and Periodicity.

Journal Article The journal of physical chemistry. C, Nanomaterials and interfaces · March 2012 The increasing popularity of surface plasmon resonance (SPR) and surface enhanced Raman scattering (SERS) sensor design based on nanotriangle or nanohole arrays, and the possibility to manufacture substrates at the transition between these plasmonic substr ... Full text Cite

Gold nanostars: surfactant-free synthesis, 3D modelling, and two-photon photoluminescence imaging.

Journal Article Nanotechnology · February 24, 2012 Understanding the control of the optical and plasmonic properties of unique nanosystems--gold nanostars--both experimentally and theoretically permits superior design and fabrication for biomedical applications. Here, we present a new, surfactant-free synt ... Full text Link to item Cite

Plasmonic gold nanostars: A potential agent for molecular imaging and cancer therapy

Journal Article Biomedical Optics, BIOMED 2012 · January 1, 2012 Gold nanostars, with tunable plasmon in the near infrared tissue optic window, generate intense two-photon photoluminescence capable of in vitro cell labeling and in vivo particle tracking. Efficient photothermal ablation therapy is also demonstrated. © 20 ... Full text Cite

Design and fabrication of fiber-optic nanoprobes for optical sensing

Journal Article Nanoscale Research Letters · January 1, 2012 This paper describes the design and fabrication of fiber-optic nanoprobes developed for optical detection in single living cells. It is critical to fabricate probes with well-controlled nanoapertures for optimized spatial resolution and optical transmissio ... Cite

Micro- and nanotopographies for photoelectrochemical energy conversion. II: Photoelectrocatalysis - Classical and advanced systems

Journal Article Electrochimica Acta · December 1, 2011 The present trends on water photolysis are shortly reviewed. For application in monolithically integrated structures, photoelectrocatalytically active half cell developments with p-Si are described. Using the concept of nanoemitter minority carrier harvest ... Full text Cite

Design and Fabrication of Fiber-Optic Nanoprobes for Optical Sensing.

Journal Article Nanoscale research letters · December 2011 This paper describes the design and fabrication of fiber-optic nanoprobes developed for optical detection in single living cells. It is critical to fabricate probes with well-controlled nanoapertures for optimized spatial resolution and optical transmissio ... Full text Cite

Plasmonic coupling interference (PCI) nanoprobes for nucleic acid detection.

Journal Article Small (Weinheim an der Bergstrasse, Germany) · November 2011 A label-free approach using plasmonic coupling interference (PCI) nanoprobes for nucleic acid detection using surface-enhanced Raman scattering (SERS) is described. To induce a strong plasmonic coupling effect, a nanonetwork of silver nanoparticles with th ... Full text Cite

Plasmonics quenching and enhancement of a fluorescing molecule outside and inside a silver metallic nanoshell

Journal Article IEEE Transactions on Nanotechnology · November 1, 2011 Analytic expressions are derived for the enhancement and quenching of a fluorescing molecule outside and in the interior of a metallic spherical nanoshell. The competing effects of enhancement and quenching are a strong function of the radial location of a ... Full text Cite

Silica-coated gold nanostars for combined surface-enhanced Raman scattering (SERS) detection and singlet-oxygen generation: a potential nanoplatform for theranostics.

Journal Article Langmuir : the ACS journal of surfaces and colloids · October 2011 This paper reports the synthesis and characterization of surface-enhanced Raman scattering (SERS) label-tagged gold nanostars, coated with a silica shell containing methylene blue photosensitizing drug for singlet-oxygen generation. To our knowledge, this ... Full text Cite

Amplification of fluorescence emission of CdSe/ZnS QDs entrapped in a sol-gel matrix, a new approach for detection of trace level of PAHs

Journal Article Sensors and Actuators, B: Chemical · September 20, 2011 In this work, CdSe/ZnS core/shell QDs with emission wavelengths of 535 nm, 545 nm, 555 nm and 575 nm were synthesized and the ligands on their surface were exchanged with mercaptopropionic acid (MPA) to make them water-soluble. Hydrophilic QDs were incorpo ... Full text Cite

Nanostructured substrates for surface plasmon resonance sensors

Journal Article 2011 International Workshop on Biophotonics, BIOPHOTONICS 2011 · August 31, 2011 Surface Plasmon Resonance (SPR) sensors are usually based on continuous thin metallic films and coupling of propagative plasmon (PP) modes using high index prism or grating. The quantitative monitoring of the surface evolution is performed by measuring eit ... Full text Cite

Activity of psoralen-functionalized nanoscintillators against cancer cells upon X-ray excitation.

Journal Article ACS nano · June 2011 We report development of a nanoparticle-based, X-ray-activated anticancer "nanodrug" composed of yttrium oxide (Y(2)O(3)) nanoscintillators, a fragment of the HIV-1 TAT peptide, and psoralen. In this formulation, X-ray radiation is absorbed by the Y(2)O(3) ... Full text Cite

Nanotechnology research and development for military and industrial applications

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · May 23, 2011 Researchers at the Army Aviation and Missile Research, Development, and Engineering Center (AMRDEC) have initiated multidiscipline efforts to develop nano-based structures and components for insertion into advanced missile, aviation, and autonomous air and ... Full text Cite

Deep UV nano-microstructuring of substrates for surface plasmon resonance imaging.

Journal Article Nanotechnology · April 2011 In this paper, we describe wafer-scale fabrication and characterization of plasmonic chips-containing different sizes and spacings of metallic micro- and nanoline structures-using deep UV lithography. Using a high dose (25 mJ cm( - 2)) and a proper lift-of ... Full text Cite

Compact point-detection fluorescence spectroscopy system for quantifying intrinsic fluorescence redox ratio in brain cancer diagnostics.

Journal Article J Biomed Opt · March 2011 We report the development of a compact point-detection fluorescence spectroscopy system and two data analysis methods to quantify the intrinsic fluorescence redox ratio and diagnose brain cancer in an orthotopic brain tumor rat model. Our system employs on ... Full text Link to item Cite

Hybrid top-down and bottom-up fabrication approach for wafer-scale plasmonic nanoplatforms.

Journal Article Small (Weinheim an der Bergstrasse, Germany) · March 2011 Full text Cite

Characterization of nanoprobe uptake in single cells: spatial and temporal tracking via SERS labeling and modulation of surface charge.

Journal Article Nanomedicine : nanotechnology, biology, and medicine · February 2011 A critical aspect for use of nanoprobes in biomedical research and clinical applications involves fundamental spatial and temporal characterization of their uptake and distribution in cells. Raman spectroscopy and two-dimensional Raman imaging were used to ... Full text Cite

Narrow groove plasmonic nano-gratings for surface plasmon resonance sensing.

Journal Article Optics express · January 2011 We present a novel surface plasmon resonance (SPR) configuration based on narrow groove (sub-15 nm) plasmonic nano-gratings such that normally incident radiation can be coupled into surface plasmons without the use of prism-coupling based total internal re ... Full text Cite

Design and Fabrication of Fiber-Optic Nanoprobes for Optical Sensing

Journal Article Nanoscale Research Letters · January 1, 2011 This paper describes the design and fabrication of fiber-optic nanoprobes developed for optical detection in single living cells. It is critical to fabricate probes with well-controlled nanoapertures for optimized spatial resolution and optical transmissio ... Full text Cite

Single-cell monitoring using fiberoptic nanosensors.

Journal Article Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · January 2011 This article is a review on the design, fabrication, and applications of fiberoptic nanosensors for in vivo monitoring of individual living cells. The nanosensors were fabricated with tapered optical fibers with distal ends having nanometer-sized diameters ... Full text Cite

Resonant infrared matrix-assisted pulsed laser evaporation of inorganic nanoparticles and organic/inorganic hybrid nanocomposites

Journal Article AIP Conference Proceedings · November 29, 2010 In this research, resonant infrared matrix-assisted pulsed laser evaporation (RIR-MAPLE) has been used to deposit different classes of inorganic nanoparticles, including bare, un-encapsulated ZnO and Au nanoparticles, as well as ligand-encapsulated CdSe co ... Full text Cite

On the behaviour of Au plasmonic nanoparticles during hydrogen evolution at p-Si

Journal Article Electrochemistry Communications · October 1, 2010 Brewster Angle Reflectometry (BAR) and spectral photocurrent analysis show that Au nanoparticles are in a resonant state during light-induced hydrogen evolution with unpolarized light near-normal incidence. Keggin-type phosphododecamolybdate (PMo12O403-) a ... Full text Cite

Plasmonics nanoprobes: detection of single-nucleotide polymorphisms in the breast cancer BRCA1 gene.

Journal Article Analytical and bioanalytical chemistry · September 2010 This paper describes the application of plasmonics-based nanoprobes that combine the modulation of the plasmonics effect to change the surface-enhanced Raman scattering (SERS) of a Raman label and the specificity of a DNA hairpin loop sequence to recognize ... Full text Cite

A novel cyanide ion sensing approach based on Raman scattering for the detection of environmental cyanides.

Journal Article Ecotoxicology and environmental safety · September 2010 This paper describes a direct optical approach based on Raman scattering for selective and sensitive detection of cyanide ions in aqueous environment without requiring time-consuming sample pretreatment and the formation of hydrogen cyanide. Due to the str ... Full text Cite

Surface-enhanced Raman scattering detection and tracking of nanoprobes: enhanced uptake and nuclear targeting in single cells.

Journal Article Applied spectroscopy · August 2010 We describe the development and application of a co-functionalized nanoprobe and biodelivery platform combining a nuclear targeting peptide (NTP) for improved cellular uptake and intracellular targeting with p-mercaptobenzoic acid (pMBA) as a surface-enhan ... Full text Cite

Investigating the plasmonics of a dipole-excited silver nanoshell: Mie theory versus finite element method.

Journal Article Nanotechnology · August 2010 This report compares COMSOL's finite element method (FEM) algorithm with the Mie theory for solving the electromagnetic fields in the vicinity of a silica-silver core-shell nanoparticle when excited by a radiating dipole. The novelty of this investigation ... Full text Cite

Investigation of synchronous fluorescence method in multicomponent analysis in tissue

Journal Article IEEE Journal on Selected Topics in Quantum Electronics · July 1, 2010 We investigate the advantages and disadvantages of the synchronous fluorescence (SF) scan method as compared with the fixed-excitation or fixed-emission fluorescence methods in analyzing biological tissue samples or tissue-like synthetic phantoms by quanti ... Full text Cite

Photoelectrocatalysis: Principles, nanoemitter applications and routes to bio-inspired systems

Journal Article Energy and Environmental Science · June 11, 2010 An overview on processes that are relevant in light-induced fuel generation, such as water photoelectrolysis or carbon dioxide reduction, is given. Considered processes encompass the photophysics of light absorption, excitation energy transfer to catalytic ... Full text Open Access Cite

Trace molecular detection via surface-enhanced Raman scattering and surface-enhanced resonance Raman scattering at a distance of 15 meters.

Journal Article Applied spectroscopy · May 2010 We report the first demonstration of surface-enhanced Raman spectroscopy (SERS) detection of para-mercapto benzoic acid (pMBA) and surface-enhanced resonance Raman spectroscopy (SERRS) detection of brilliant cresyl blue (BCB) and cresyl violet perchlorate ... Full text Cite

A highly sensitive Raman method for selective cyanide detection based on evaporated cuprous iodide substrate

Journal Article Analytical Methods · May 1, 2010 A strong interaction between cyanide anion and copper(i) cation in combination with non-resonant Raman fingerprinting allows the selective sensing of aqueous free cynanide with high sensitivity to parts per billion (ppb)-level. © The Royal Society of Chemi ... Full text Open Access Cite

Cellular uptake and photodynamic activity of protein nanocages containing methylene blue photosensitizing drug.

Journal Article Photochemistry and photobiology · May 2010 This study reports that photosensitizers encapsulated in supramolecular protein cages can be internalized by tumor cells and can deliver singlet oxygen intracellularly for photodynamic therapy (PDT). As an alternative to other polymeric and/or inorganic na ... Full text Cite

Plasmonic Nanoparticles and Nanowires: Design, Fabrication and Application in Sensing.

Journal Article The journal of physical chemistry. C, Nanomaterials and interfaces · April 2010 This study involves two aspects of our investigations of plasmonics-active systems: (i) theoretical and simulation studies and (ii) experimental fabrication of plasmonics-active nanostructures. Two types of nanostructures are selected as the model systems ... Full text Open Access Cite

Methodologies for developing surface-enhanced raman scattering (SERS) substrates for detection of chemical and biological molecules

Journal Article IEEE Sensors Journal · March 1, 2010 This paper describes methodologies for developing efficient surface-enhanced Raman scattering (SERS) substrates such as annealing thin gold films for developing gold nanoislands, fabrication of nanopillars arrays and roughened films by employing focused io ... Full text Cite

Plasmonic nanoprobes for SERS biosensing and bioimaging.

Journal Article Journal of biophotonics · January 2010 This article provides an overview of the development and application of plasmonic nanoprobes developed in our laboratory for biosensing and bioimaging. We describe the use of plasmonics surface-enhanced Raman scattering (SERS) gene probes for the detection ... Full text Cite

Scanning probe characterization of enzymes deposited onto step-bunched silicon nanostructures

Journal Article Physica Scripta · November 27, 2009 Step-bunched Si surfaces with step heights of ∼3 nm have been prepared as substrates for immobilization of the enzyme reverse transcriptase of the avian myeloblastosis virus. Tapping mode atomic force microscopy has been used for imaging. Calibration exper ... Full text Cite

Nano-biophotonics: From laboratory research to biomedical diagnostics

Journal Article 2009 1st Annual ORNL Biomedical Science and Engineering Conference, BSEC 2009 · October 21, 2009 This lecture presents an overview of recent advances in the development of nano-biophotonic technologies including nanosensors and nanoprobes at the nexus of biology, medicine and nanotechnology [1]-[5]. This presentation describes two areas of research re ... Full text Cite

Spectral filtering modulation method for estimation of hemoglobin concentration and oxygenation based on a single fluorescence emission spectrum in tissue phantoms.

Journal Article Medical physics · October 2009 PurposeHemoglobin concentration and oxygenation in tissue are important biomarkers that are useful in both research and clinical diagnostics of a wide variety of diseases such as cancer. The authors aim to develop simple ratiometric method based o ... Full text Cite

Fiber optic SERS-based plasmonics nanobiosensing in single living cells

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · September 8, 2009 We describe the development of small molecule-sensitive plasmonics-active fiber-optic nanoprobes suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The practical utility of SERS-bas ... Full text Cite

Demonstration of surface-enhanced Raman scattering by tunable, plasmonic gallium nanoparticles.

Journal Article Journal of the American Chemical Society · September 2009 Size-controlled gallium nanoparticles deposited on sapphire were explored as alternative substrates to enhance Raman spectral signatures. Gallium's resilience following oxidation is inherently advantageous in comparison with silver for practical ex vacuo n ... Full text Cite

Plasmonics of 3-D nanoshell dimers using multipole expansion and finite element method.

Journal Article ACS nano · September 2009 The spatial and spectral responses of the plasmonic fields induced in the gap of 3-D nanoshell dimers of gold and silver are comprehensively investigated and compared via theory and simulation using the multipole expansion (ME) and the finite element metho ... Full text Cite

Plasmonics enhancement of a luminescent or Raman-active layer in a multilayered metallic nanoshell.

Journal Article Applied optics · September 2009 Expressions for the enhancement of the far-field scattering cross section of a luminescent or Raman-active compound contained within a multilayered nanosphere are derived, where the active compound resides between an outer metallic shell and a metallic cor ... Full text Cite

Applications of fiber-optics-based nanosensors to drug discovery.

Journal Article Expert opinion on drug discovery · August 2009 BackgroundFiber-optic nanosensors are fabricated by heating and pulling optical fibers to yield sub-micron diameter tips and have been used for in vitro analysis of individual living mammalian cells. Immobilization of bioreceptors (e.g., antibodie ... Full text Cite

Fabrication of nanodot plasmonic waveguide structures using FIB milling and electron beam-induced deposition.

Journal Article Scanning · July 2009 Fabrication of metallic Au nanopillars and linear arrays of Au-containing nanodots for plasmonic waveguides is reported in this article by two different processes-focused ion beam (FIB) milling of deposited thin films and electron beam-induced deposition ( ... Full text Cite

FIB/SEM fabrication of nanostructures for plasmonic sensors and waveguides

Journal Article Microscopy and Microanalysis · July 1, 2009 Full text Cite

Comparison of FDTD numerical computations and analytical multipole expansion method for plasmonics-active nanosphere dimers.

Journal Article Optics express · June 2009 This paper describes a comparative study of finite-difference time-domain (FDTD) and analytical evaluations of electromagnetic fields in the vicinity of dimers of metallic nanospheres of plasmonics-active metals. The results of these two computational meth ... Full text Cite

Fabrication of metallic nanodot structures using focused ion beam (Fib) and electron Beam-induced deposition for plasmonic waveguides

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · May 25, 2009 Fabrication of a linear array of metallic nanodots and nanopillars for plasmonic waveguides is reported in this paper by two different processes - FIB milling of deposited thin films and electron beam-induced deposition of metallic nanostructures from a or ... Full text Cite

Fabrication of novel plasmonics-active substrates

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · May 5, 2009 This paper describes methodologies for fabricating of highly efficient plasmonics-active SERS substrates - having metallic nanowire structures with pointed geometries and sub-5 nm gap between the metallic nanowires enabling concentration of high EM fields ... Full text Cite

Multiplex detection of breast cancer biomarkers using plasmonic molecular sentinel nanoprobes

Journal Article Nanotechnology · February 2009 We have demonstrated for the first time the feasibility of multiplex detection using the surface-enhanced Raman scattering-based molecular sentinel ( MS) technology in a homogeneous solution. Two MS nanoprobes tagged with different Raman labels were used t ... Cite

SERS-based plasmonic nanobiosensing in single living cells.

Journal Article Analytical and bioanalytical chemistry · February 2009 In this paper, we describe the development and application of a pH-sensitive plasmonics-active fiber-optic nanoprobe suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The effective ... Full text Cite

Development of plasmonics-active SERS substrates on a wafer scale for chemical and biological sensing applications

Journal Article Technical Digest - International Electron Devices Meeting, IEDM · December 1, 2008 This paper describes the fabrication of highly efficient plasmonics-active SERS substrates - having metallic nanowire structures with pointed geometries and sub-5 nm gap between the metallic nanowires enabling concentration of high EM fields in these regio ... Full text Cite

Plasmonic SERS molecular sentinels: A new biosensing approach

Journal Article · December 1, 2008 This chapter describes the detection of specific target DNA sequences using a novel 'molecular sentinel' (MS) biosensing approach using surface-enhanced Raman scattering (SERS) detection. The SERS-based MS nanoprobe consists of a metal nanoparticle and a s ... Full text Cite

Optical response of linear chains of metal nanospheres and nanospheroids.

Journal Article Journal of the Optical Society of America. A, Optics, image science, and vision · November 2008 A semi-analytical method for computing the electric field surrounding a finite linear chain of metal nanospheres and nanospheroids is described. In treating chains or clusters of spheres, a common approach is to use the spherical-harmonic addition theorem ... Full text Cite

Apoferritin protein cages: a novel drug nanocarrier for photodynamic therapy.

Journal Article Chemical communications (Cambridge, England) · October 2008 A methylene blue-encapsulated apoferritin complex shows cytotoxic effects on MCF-7 human breast adenocarcinoma cells when irradiated at the appropriate wavelength. ... Full text Cite

Nanosensors and nanoprobes for environmental health sensing and biomedical screening

Journal Article LEOS Summer Topical Meeting · September 22, 2008 Full text Cite

Computational design of FIB-milled nanostructures for use in biosensing

Journal Article LEOS Summer Topical Meeting · September 22, 2008 Full text Cite

Spectral bounds on plasmon resonances for Ag and Au prolate and oblate nanospheroids.

Journal Article Journal of nanophotonics · September 2008 Analytical expressions for the plasmon resonance frequencies of prolate and oblate spheroids and their dependence on ellipticity have been derived, and approximate bounds on these frequencies established. These formulas may be useful in tuning the plasmon ... Full text Cite

Modeling of nonphase mechanisms in ultrasonic modulation of light propagation.

Journal Article Applied optics · July 2008 While phase variation due to ultrasonic modulation of coherent light has been extensively studied in acousto-optical imaging, fewer groups have studied nonphase mechanisms of ultrasonic modulation, which may be important in exploring ultrasonic modulation ... Full text Cite

Focus on bioanalysis

Journal Article Analytical and Bioanalytical Chemistry · July 1, 2008 The guest editors of Analytical and Bioanalytical Chemistry (ABC) invited scientific professionals, to submit review papers on new developments in bioanalysis. The guest editors reviewed articles related to detection principles, surveying immunoassays and ... Full text Cite

Intensified biochip system using chemiluminescence for the detection of Bacillus globigii spores.

Journal Article Analytical and bioanalytical chemistry · July 2008 This paper reports the first intensified biochip system for chemiluminescence detection and the feasibility of using this system for the analysis of biological warfare agents is demonstrated. An enzyme-linked immunosorbent assay targeting Bacillus globigii ... Full text Cite

Plasmonic SERS Molecular Sentinels. A New Biosensing Approach

Chapter · April 29, 2008 This chapter describes the detection of specific target DNA sequences using a novel 'molecular sentinel' (MS) biosensing approach using surface-enhanced Raman scattering (SERS) detection. The SERS-based MS nanoprobe consists of a metal nanoparticle and a s ... Full text Cite

Modeling of amplitude and exit location variations of light propagation under ultrasonic modulation

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 21, 2008 While phase variation due to ultrasonic modulation of coherent light has been extensively studied in acousto-optical imaging, fewer groups have studied non-phase mechanisms of ultrasonic modulation, which may be important in exploring ultrasonic modulation ... Full text Cite

Theoretical modeling of plasmon resonances: Effect of aspect ratio in spheroidal nanoshells

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 21, 2008 Plasmon resonances are computed for prolate spheroidal nanoshells. Both longitudinal and transverse resonances are investigated as a function of aspect ratio. Formulas for the surface charge density on the outside and inside shell surfaces are derived. ... Full text Cite

Nano-engineered surface-enhanced Raman scattering (SERS) substrates with patterned structures on the distal end of optical fibers

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 21, 2008 This paper describes the development of fiber optic sensor probes and planar substrates containing patterned nanostructures such as nanoholes in gold films, as well as gold nanoparticles, nano-pillars, nanorods, and nano-islands. Several methods of produci ... Full text Cite

Detection of multidrug-resistant tuberculosis gene using plasmonics nanoprobes

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 21, 2008 This paper describes the use of plasmonics-based nanoprobes for detection of multidrug-resistant tuberculosis gene. The plasmonics nanoprobe is composed of a silver nanoparticle pre-coated with a stem-loop DNA probe that is tagged with a Raman label at one ... Full text Cite

Geometry-dependent plasmon resonances of metallic nanostructures for enhancement of localized electromagnetic fields around the nanostructures

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · March 21, 2008 This paper describes theoretical and experimental evaluations of electromagnetic fields around metallic nanostructures, such as nanorods, nano-pillars, and a collection of nanorods separated by nano-scale distances. Nanostructures having different sizes an ... Full text Cite

Silver nanoparticle-doped polyvinyl alcohol coating as a medium for surface-enhanced Raman scattering analysis.

Journal Article Journal of nanoscience and nanotechnology · February 2008 A simple polymer substrate for inducing Surface-Enhanced Raman Scattering (SERS) has been investigated. This SERS substrate consists of a solid support, such as a glass slide covered with polyvinyl alcohol (PVA) impregnated with fine silver nanoparticles. ... Full text Cite

Nanosensing at the single cell level.

Journal Article Spectrochimica acta. Part B, Atomic spectroscopy · February 2008 This article presents an overview of the development, operation, and applications of optical nanobiosensors for use in in vivo detection of biotargets in individual living cells. The nanobiosensors are equipped with immobilized bioreceptor probes (e ... Full text Cite

Fiberoptics Nanosensing at the Single Cell Level

Journal Article Spectrochimia Acta B · 2008 Cite

Photodynamic therapy of Barrett's esophagus: ablation of Barrett's mucosa and reduction in p53 protein expression after treatment.

Journal Article Anticancer research · January 2008 BackgroundThe effectiveness of photodynamic therapy (PDT) for ablation of high grade dysplasia (HGD) in Barrett's esophagus (BE) is typically reported histologically. Following successful PDT, Barrett's mucosa is replaced with neosquamous mucosa. ... Cite

Nanobiosensing Using Plasmonic Nanoprobes.

Journal Article IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society · January 2008 This paper provides an overview of the development and applications of plasmonics-active nanoprobes in biomedical diagnostics. Specific examples of detection techniques using surface-enhanced Raman scattering are presented to illustrate the usefulness and ... Full text Cite

FIB Fabrication of Metallic Nanostructures on End-Faces of Optical Fibers for Chemical Sensing Applications.

Journal Article Journal of vacuum science & technology. B, Microelectronics and nanometer structures : processing, measurement, and phenomena : an official journal of the American Vacuum Society · January 2008 Full text Cite

Gold Nanostars For Surface-Enhanced Raman Scattering: Synthesis, Characterization and Optimization.

Journal Article The journal of physical chemistry. C, Nanomaterials and interfaces · January 2008 The controlled synthesis of high-yield gold nanostars of varying sizes, their characterization and use in surface-enhanced Raman scattering (SERS) measurements are reported for the first time. Gold nanostars ranging from 45 to 116-nm in size were synthesiz ... Full text Cite

Biosensors and biochips

Journal Article · December 1, 2007 This chapter provides an overview of the various types of biosensors and biochips that have been developed for biological and medical applications, along with significant advances and over the last several years in these technologies. Various classificatio ... Full text Cite

Theoretical Simulation and Focused Ion Beam Fabrication of Gold Nanostructures For Surface-Enhanced Raman Scattering (SERS).

Journal Article Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences · December 2007 This paper describes the fabrication of gold nanopillar and nanorod arrays and theoretical calculations of electromagnetic fields (EMFs) around ordered arrangements of these nanostructures. The EMFs of both single nanopillars and di-mers of nanopillars - h ... Full text Cite

Nanobiosensing using plasmonics nanoprobes

Journal Article Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS · December 1, 2007 Full text Cite

Tracking SERS-active nanoprobe intracellular uptake for chemical and biological sensing

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 2007 A critical aspect of the use of nanoprobes for intracellular studies in chemical and biological sensing involves a fundamental understanding of their uptake and trajectory in cells. In this study, we describe experiments using surfaceenhanced Raman scatter ... Full text Cite

Development of a synchronous fluorescence imaging system and data analysis methods.

Journal Article Optics express · October 2007 Although conventional autofluorescence spectroscopy, in which fluorescence emission spectra are recorded for fixed excitation wavelengths, has demonstrated good performance in tissue diagnosis, it suffers from prolonged data acquisition time and broad-band ... Full text Cite

Imaging the distribution of magnetic nanoparticles with ultrasound.

Journal Article IEEE transactions on medical imaging · May 2007 Magnetic nanoparticles can be caused to oscillate under the influence of an incident ultrasonic wave. If the particles are momentarily aligned with a magnetizing pulse creating a macroscopic magnetization, this oscillation will result in a time-varying mag ... Full text Cite

Surface-enhanced Raman scattering for identification of organic pigments and dyes in works of art and cultural heritage material

Journal Article Sensor Review · April 8, 2007 Purpose - Identification and characterization of organic pigments and dyes used in works of art and cultural heritage material such as prints, drawings, manuscripts, paintings, and textiles can provide important information for dating, authentication, and ... Full text Cite

Surface-enhanced Raman scattering detection of chemical and biological agents using a portable Raman integrated tunable sensor

Journal Article Sensors and Actuators, B: Chemical · January 30, 2007 This paper describes a compact and rugged Raman integrated tunable sensor coupled with surface-enhanced Raman scattering substrates for the screening of a wide variety of chemical and biological agents for homeland defense applications. The field-deployabl ... Full text Cite

Extracting intrinsic synchronous fluorescence for spectral imaging

Journal Article Optics InfoBase Conference Papers · January 1, 2007 A ratio-metric method was proposed to extract intrinsic fluorescence from synchronous fluorescence spectra distorted by absorption and scattering. Its applicable range was investigated using Monte Carlo simulations and the accuracy was tested with phantom ... Cite

Extracting intrinsic synchronous fluorescence for spectral imaging

Journal Article Optics InfoBase Conference Papers · January 1, 2007 A ratio-metric method was proposed to extract intrinsic fluorescence from synchronous fluorescence spectra distorted by absorption and scattering. Its applicable range was investigated using Monte Carlo simulations and the accuracy was tested with phantom ... Cite

Extracting intrinsic synchronous fluorescence for spectral imaging

Journal Article Optics InfoBase Conference Papers · January 1, 2007 A ratio-metric method was proposed to extract intrinsic fluorescence from synchronous fluorescence spectra distorted by absorption and scattering. Its applicable range was investigated using Monte Carlo simulations and the accuracy was tested with phantom ... Cite

Extracting intrinsic synchronous fluorescence for spectral imaging

Conference Optics InfoBase Conference Papers · January 1, 2007 A ratio-metric method was proposed to extract intrinsic fluorescence from synchronous fluorescence spectra distorted by absorption and scattering. Its applicable range was investigated using Monte Carlo simulations and the accuracy was tested with phantom ... Full text Cite

Plasmon Resonances of Nanoshells of Spheroidal Shape.

Journal Article IEEE transactions on nanotechnology · January 2007 Plasmon resonances are computed for nanoshells of prolate and oblate spheroidal shape. Both longitudinal and transverse resonances are investigated as a function of aspect ratio and shell thickness. Formulas for the surface charge density on the outside an ... Full text Cite

Fabrication of fiber-optic sensors based on plasmon resonances of metallic nanostructures

Journal Article ECS Transactions · January 1, 2007 This paper describes the fabrication of portable and robust fiber optic sensors - mainly chemical and biological sensors - on standard communication grade optical fibers. Metallic nanostructures such as nanoparticles, nanopillars, nanorods, and nanoholes i ... Full text Cite

Time-resolved optical tomography using short-pulse laser for tumor detection.

Journal Article Applied optics · August 2006 Our objective is to perform a comprehensive experimental and numerical analysis of the short-pulse laser interaction with a tissue medium with the goal of tumor-cancer diagnostics. For a short-pulse laser source, the shape of the output signal is a functio ... Full text Cite

Hyperspectral imaging system using acousto-optic tunable filter for flow cytometry applications.

Journal Article Cytometry. Part A : the journal of the International Society for Analytical Cytology · August 2006 A major advantage of flow cytometry is its flexible and open instrument configuration, which is highly suitable for systems integration. This flexibility permits the coupling of auxiliary instrumentation that may offer the measurement of parameters other t ... Full text Cite

Self-assembly of silver nanoparticles: synthesis, stabilization, optical properties, and application in surface-enhanced Raman scattering.

Journal Article The journal of physical chemistry. B · July 2006 Silver nanoparticle aggregates were synthesized in large scale using resorcinol under alkaline condition to obtain an assembly of silver clusters. Stable dispersion of the cluster in aqueous medium has been examined out of resorcinol-capped silver nanopart ... Full text Cite

Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection.

Journal Article Annals of biomedical engineering · June 2006 An advanced hyper-spectral imaging (HSI) system has been developed having obvious applications for cancer detection. This HSI system is based on state-of-the-art liquid crystal tunable filter technology coupled to an endoscope. The goal of this unique HSI ... Full text Cite

Direct identification of alizarin and lac dye on painting fragments using surface-enhanced Raman scattering

Journal Article Analytica Chimica Acta · May 31, 2006 A surface-enhanced Raman scattering (SERS) based procedure was investigated for potential applications in the direct identification of selected anthraquinone dyes on works of art objects. The simplicity and effectiveness of this procedure was demonstrated ... Full text Cite

Hyperspectral fluorescence imaging system for biomedical diagnostics

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · May 8, 2006 An advanced hyper-spectral imaging (HSI) system has been developed for use in medical diagnostics. One such diagnostic, esophageal cancer is diagnosed currently through biopsy and subsequent pathology. The end goal of this research is to develop an optical ... Full text Cite

Nanoprobes and nanobiosensors for monitoring and imaging individual living cells.

Journal Article Nanomedicine : nanotechnology, biology, and medicine · March 2006 This article presents an overview of nanobiosensors and nanoprobes. The fabrication, operating principle, and applications of these systems and their capacity for in vivo analysis and optical imaging at the single-cell level are discussed. Recently the cro ... Full text Cite

An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging.

Journal Article Medical engineering & physics · March 2006 An acousto-optic tunable filter (AOTF)-based system for dual-modality hyperspectral imaging (DMHSI) has been developed for use in characterization of normal and malignant mouse tissue. The system consists of a laser, endoscope, AOTF, and two cameras couple ... Full text Cite

Antibody-based SERS diagnostics of Fhit protein without label

Journal Article Nanobiotechnology · March 1, 2006 Surface-enhanced Raman scattering (SERS) is a particularly promising technique that has the potential to perform highly selective and sensitive in situ measurements of antibody-antigen reactions. This work describes the use of silver (Ag) colloids for immu ... Full text Cite

FRET-based protein-DNA binding assay for detection of active NF-κB

Journal Article Sensors and Actuators, B: Chemical · February 27, 2006 A novel method to detect the active form of NF-κB, a transcription factor regulating a battery of inflammatory genes and playing a fundamental role in the development of numerous pathological states, has been developed. In the present work, we used fluores ... Full text Cite

Plasmonics and surface-enhanced raman scattering (SERS) nanoprobes for biomedical diagnostics

Journal Article Optics InfoBase Conference Papers · January 1, 2006 Cite

Plasmonics and surface-enhanced raman scattering (SERS) nanoprobes for biomedical diagnostics

Journal Article Optics InfoBase Conference Papers · January 1, 2006 Cite

Plasmonics and surface-enhanced Raman scattering (SERS) nanoprobes for biomedical diagnostics

Journal Article Optics InfoBase Conference Papers · January 1, 2006 Cite

Plasmonics and surface-enhanced Raman scattering (SERS) nanoprobes for biomedical diagnostics

Journal Article Optics InfoBase Conference Papers · January 1, 2006 Cite

Short-pulse laser propagation through tissue medium for tumor detection

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2006 The objective of this paper is to perform a comprehensive experimental and numerical analysis of the short pulse laser interaction with tissue medium with the goal of tumor / cancer diagnostics. For short pulse laser source, the shape of output signal is a ... Full text Cite

Synthesis and characterization of SERS gene probe for BRCA-1 (breast cancer).

Journal Article Faraday discussions · January 2006 A protocol for binding cresyl fast violet (CFV), a SERS-active dye (label) containing an aromatic amino group with a modified oligomer having a carboxy derivatized thymidine moiety using carbodiimide coupling has been achieved for the first time. Covalent ... Full text Cite

Hyperspectral fluorescence imaging for mouse skin tumor detection

Journal Article ETRI Journal · January 1, 2006 This paper presents a hyperspectral imaging technique based on laser-induced fluorescence for non-invasive detection of tumorous tissue on mouse skin. Hyperspectral imaging sensors collect image data in a number of narrow, adjacent spectral bands. Such hig ... Full text Cite

Neutron spectrometry using CR-39 track etch detectors.

Journal Article Radiation protection dosimetry · January 2006 Track-size distributions were measured for chemically etched CR-39 foils exposed to monoenergetic neutrons with energies ranging from 0.144 to 19 MeV and to various broad-spectrum neutron sources including spontaneous fission neutrons from (238)Pu. These t ... Full text Cite

Application of surface-enhanced Raman scattering (SERS) for the identification of anthraquinone dyes used in works of art

Journal Article Journal of Raman Spectroscopy · January 1, 2006 Surface-enhanced Raman scattering (SERS) was investigated for applications in the analysis of anthraquinone dyes used in works of art. Two SERS procedures were developed and evaluated with three frequently used anthraquinone dyes, alizarin, carminic acid a ... Full text Cite

FRET based biosensor for detection of active NF-kB

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 13, 2005 The Nuclear Factor kB is a transcription factor, ubiquitously expressed, involved in the regulation of a large number of genes and in a variety of human disease including inflammation, asthma, atherosclerosis, AIDS, septic shock, arthritis and cancer. The ... Full text Cite

Proceedings of SPIE - The International Society for Optical Engineering: Introduction

Conference Proceedings of SPIE - The International Society for Optical Engineering · December 1, 2005 Cite

Detection of human immunodeficiency virus type 1 DNA sequence using plasmonics nanoprobes.

Journal Article Analytical chemistry · December 2005 This paper describes the use of plasmonics-based nanoprobes that act as molecular sentinels for DNA diagnostics. The plasmonics nanoprobe comprises a metal nanoparticle and a stem-loop DNA molecule tagged with a Raman label. The nanoprobe utilizes the spec ... Full text Cite

Optical nanobiosensor for monitoring an apoptotic signaling process in a single living cell following photodynamic therapy.

Journal Article Journal of nanoscience and nanotechnology · December 2005 Optical nanobiosensors have enabled bioanalytical measurements to be undertaken within volumes as small as that of single biological cells. In this work, we use nanobiosensors to monitor a molecular signaling process, i.e., caspase-7 activation, following ... Full text Cite

Application of surface-enhanced Raman scattering (SERS) for the identification of anthraquinone dyes used in works of art

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 2005 Surface-enhanced Raman scattering (SERS) was investigated for applications in the analysis of anthraquinone dyes used in works of art. Two SERS procedures were developed and evaluated with frequently used anthraquinone dyes, alizarin, carminic acid and lac ... Full text Cite

Portable AOTF Raman Integrated Tunable Sensor (RAMiTS) for chemical and biosensing

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 2005 This paper describes the development of a compact, self-contained, and portable Raman Integrated Tunable Sensor (RAMiTS) for chemical and biosensing. The RAMiTS consists of a frequency-stabilized diode laser for excitation, an acousto-optic tunable filter ... Full text Cite

NanoBiotechnology: Editorial

Journal Article Nanobiotechnology · December 1, 2005 Full text Cite

Welcome to NanoBio Euro 2005

Journal Article Nanobiotechnology · December 1, 2005 Full text Cite

Applications of carbon nanotubes for cancer research

Journal Article Nanobiotechnology · December 1, 2005 Carbon nanotubes have many unique properties such as high surface area, hollow cavities, and excellent mechanical and electrical properties. Interfacing carbon nanotubes with biological systems could lead to significant applications in various disease diag ... Full text Cite

Photothermal treatment of human carcinoma cells using liposome-encapsulated gold nanoshells

Journal Article Nanobiotechnology · December 1, 2005 We report the application of liposome-encapsulated gold nanoshells for in vitro photo-induced hyperthermia in human mammary carcinoma cells. In addition to evaluating their effects in vitro, we compared the application liposome-encapsulated gold nanoshells ... Full text Cite

Introduction to NanoBiotechnology

Journal Article Nanobiotechnology · September 19, 2005 Full text Cite

Instrumentation considerations in spectral imaging for tissue demarcation: Comparing three methods of spectral resolution

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · August 16, 2005 Multiple methodologies exist to implement spectral imaging for tissue demarcation and disease diagnosis. In this paper, benchtop acousto-optic tunable filter (AOTF), liquid-crystal tunable filter (LCTF) and Fourier interferometric spectral imaging systems ... Full text Cite

Surface-enhanced Raman scattering detection of chemical and biological agent simulants

Journal Article IEEE Sensors Journal · August 1, 2005 Surface-enhanced Raman scattering spectra of chemical and biological agent simulants, such as dimethyl methylphonate, pinacolyl methylphosphonate, diethyl phosphoramidate, 2-chloroethyl ethylsulfide, bacillus globigii, erwinia herbicola, and bacillus thuri ... Full text Cite

Portable Raman device for detection of chemical and biological warfare agents

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · July 21, 2005 This paper describes a compact, self-contained, cost effective, and portable Raman Integrated Tunable Sensor (RAMiTs) for screening a wide variety of chemical and biological agents for homeland defense applications. The instrument is a fully-integrated, tu ... Full text Cite

Hyperspectral fluorescence image analysis for use in medical diagnostics

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · July 21, 2005 This paper presents hyperspectral fluorescence imaging and a support vector machine for detecting skin tumors. Skin cancers may not be visually obvious since the visual signature appears as shape distortion rather than discoloration. As a definitive test f ... Full text Cite

Surface-enhanced Raman scattering molecular nanoprobes

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · July 21, 2005 Nanoparticles are increasingly finding a wide application in the biological studies due to their unique physical and chemical properties. Colloidal nanoparticles are efficient substrate that exhibit surface-enhanced Raman scattering (SERS) phenomenon by en ... Full text Cite

Dual modality fluorescence and reflectance Hyperspectral imaging: Principle and applications

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · July 21, 2005 We present the principles and applications of our dual-modality fluorescence and reflectance hyperspectral imaging (DMHSI) system. In this paper we report on background work done using laser induced fluorescence (LIF) by the group in the early detection of ... Full text Cite

Progress in Biomedical Optics and Imaging - Proceedings of SPIE: Introduction

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · July 21, 2005 Cite

Near-real-time determination of hydrogen peroxide generated from cigarette smoke.

Journal Article Journal of environmental monitoring : JEM · July 2005 The ability to monitor hydrogen peroxide (H2O2) in aqueous smoke extracts will advance our understanding of the relationship between cigarette smoke-induced oxidative stress, inflammation, and disease and help elucidate the pathways by which the various sm ... Full text Cite

Hyperspectral surface-enhanced Raman imaging of labeled silver nanoparticles in single cells

Journal Article Review of Scientific Instruments · June 28, 2005 We describe the development of an acousto-optic tunable filter (AOTF) -based hyperspectral surface-enhanced Raman imaging (HSERI) system equipped with an intensified charged coupled device and an avalanche photodiode. The AOTF device is a miniature rapid-s ... Full text Cite

Fiber-optic nanosensors for single-cell monitoring.

Journal Article Analytical and bioanalytical chemistry · June 2005 This article is an overview of the fabrication, operating principles, and applications of fiber-optic nanobiosensors with the capability of in-vivo analysis at the single-cell level. Recently, the cross-disciplinary integration of nanotechnology, biology, ... Full text Cite

A sol-gel derived AgCl photochromic coating on glass for SERS chemical sensor application

Journal Article Sensors and Actuators, B: Chemical · May 13, 2005 A new optically translucent material has been prepared that acts as a substrate for surface-enhanced Raman spectroscopy. This material is a silica matrix, synthesized by the sol-gel method and containing in situ precipitated AgCl particles which serve as p ... Full text Cite

A compact CMOS biochip immunosensor towards the detection of a single bacteria.

Journal Article Biosensors & bioelectronics · May 2005 Recent use of biological warfare (BW) agents has led to a growing interest in the rapid and sensitive detection of pathogens. Therefore, the development of field-usable detection devices for sensitive and selective detection of BW agents is an important is ... Full text Cite

A comparison of enhancement factors for surface-enhanced Raman scattering using visible and near-infrared excitations

Journal Article Journal of Molecular Structure · February 14, 2005 We investigated enhancement factors for surface-enhanced Raman scattering from p-aminobenzoic acid (PABA) on different silver and gold substrates using visible (632.8 nm) and near-infrared (NIR) (1064 nm) excitations. The results indicated that the use of ... Full text Cite

Surface-enhanced Raman scattering for medical diagnostics and biological imaging

Journal Article Journal of Raman Spectroscopy · January 1, 2005 We present recent development and applications of a surface-enhanced Raman scattering (SERS) technology for use in medical diagnostics and biological imaging. For medical diagnostics, we use Raman-active dye-labeled DNA gene probes and nanostructured metal ... Full text Cite

Liposome encapsulated gold nanoshells for NanoPhototherapy induced hyperthermia

Journal Article International Journal of Nanotechnology · January 1, 2005 Gold nanoshells have been extensively studied since their invention and their role in both in vitro and in vivo photothermal therapy has been explored and demonstrated. In this work, we investigate liposomal delivery of gold nanoshells and evaluate their e ... Full text Cite

Plasmonics-based nanostructures for surface-enhanced Raman scattering bioanalysis.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2005 Surface-enhanced Raman scattering (SERS) spectroscopy is a plasmonics-based spectroscopic technique that combines modern laser spectroscopy with unique optical properties of metallic nanostructures, resulting in strongly increased Raman signals when molecu ... Full text Cite

Studying 3D subdomains of proteins at the nanometer scale using fluorescence spectroscopy.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2005 Databases devoted to the crystal structure of proteins have dramatically increased in size during the last two decades. Moreover, X-ray and NMR technology studies have shown that proteins belonging to the same family generally share the same global 3D arch ... Full text Cite

Near-field scanning optical microscopy for bioanalysis at nanometer resolution.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2005 The nondestructive imaging of biomolecules in nanometer domains in their original location and position as adsorbed or deposited on a surface is of garners considerable experimental interest. Near-field scanning optical microscopy (NSOM) is an emerging tec ... Full text Cite

Protein nanotechnology: the new frontier in biosciences.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2005 The combination of nanotechnology and molecular biology has led to a new generation of nanoscale-based devices and methods for probing the cell machinery and elucidating intimate life processes occurring at the molecular level that were heretofore invisibl ... Full text Cite

Optical nanosensors for detecting proteins and biomarkers in individual living cells.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2005 Recently, nanotechnology has been revolutionizing important areas in molecular biology and medicine, especially diagnostics and therapy at the molecular and cellular levels. The combination of nanotechnology, biology, advanced materials, and photonics open ... Full text Cite

Protein amyloidose misfolding: mechanisms, detection, and pathological implications.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2005 A variety of diseases result because of misfolded protein that deposits in extracellular space in the body. These deposits can be amorphous (disordered) or fibrillar (ordered). Inclusion bodies are an example of amorphous aggregates, and amyloid fibril is ... Full text Cite

Introduction

Journal Article Advances in the Economics of Environmental Resources · January 1, 2005 Full text Cite

Miniature biochip system for bioenvironmental applications

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 2004 An integrated multi-functional biochip based on integrated circuit complementary metal oxide semiconductor (CMOS) sensor array for use in medical diagnostics and pathogen detection has been described. The usefulness and potential of the biochip as a rapid, ... Full text Cite

Portable Raman Integrated Tunable Sensor (RAMiTs) for environmental field monitoring

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 2004 This paper describes a self-contained, portable Raman instrument that has been developed for environmental and homeland defense applications. The instrument consists of a 830-nm diode laser for excitation, an acousto-optic tunable filter (AOTF) for wavelen ... Full text Cite

Advanced biophotonics: From biochips to nanosensors

Journal Article Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004 · December 1, 2004 This lecture provides an overview of the research activities in our laboratory related to the development and application of advanced biomedical photonics, molecular spectroscopy, nanosensors and biochips for biomedical diagnostics. © 2004 IEEE. ... Cite

Medical biophotonics: From lasers to blochips

Journal Article Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS · December 1, 2004 Medical use of biophotonics for therapeutic and diagnostic purposes was discussed. Nanoparticle based surface-enhanced Raman scattering (SERS) technology enabled sensitive detection of a variety of compounds of environmental and medical interest. Biosensor ... Cite

Metallic nanostructures for plasmonic sensors using surface-enhanced fluorescence and Raman detection

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · November 16, 2004 We present the fabrication and characterization of silver island films with SiO 2 coatings for application in plasmonic sensors based on surface-enhanced fluorescence and Raman detection. The emission spectral properties of goat anti-mouse immunoglobulin ( ... Full text Cite

Surface-enhanced Raman scattering (SERS) detection for chemical and biological agents

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · October 27, 2004 Surface-enhanced Raman scattering (SERS) spectra of chemical agent simulants such as dimethyl methylphonate (DMMP), pinacolyl methylphosphonate (PMP), diethyl phosphoramidate (DEPA), and 2-chloroethyl ethylsulfide (CEES), and biological agent simulants suc ... Full text Cite

A hyperspectral imaging system for in vivo optical diagnostics. Hyperspectral imaging basic principles, instrumental systems, and applications of biomedical interest.

Journal Article IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society · September 2004 Full text Cite

Photochemically prepared gold metal film in a carbohydrate-based polymer: A practical solid substrate for surface-enhanced Raman scattering

Journal Article Current Science · August 25, 2004 A method for the preparation of thin gold film on glass slides has been described. When thin films of methylcellulose containing chloroauric acid are irradiated in a photoreactor under UV light, they produce a clear gold mirror made of aggregated gold part ... Cite

Investigation of microfabrication of biological sample arrays using piezoelectric and bubble-jet printing technologies

Journal Article Analytica Chimica Acta · August 2, 2004 Modified commercial ink-jet printers are simple and cost-effective tools for the fabrication of microarrays containing biological samples. In this paper, the two most common ink-dispensing technologies, thermal and piezoelectric, were investigated, tested, ... Full text Cite

Single-board computer based control system for a portable Raman device with integrated chemical identification

Journal Article Review of Scientific Instruments · June 1, 2004 A battery-powered portable chemical identification device built around an acousto-optic tunable filter (AOTF)-based Raman spectrometer was developed. The system was controlled by custom-developed software that works on an embedded single-board computer (SB ... Full text Cite

Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces.

Journal Article Journal of biomedical optics · May 2004 We describe the use of a biochip based on complementary metal oxide semiconductor (CMOS) technology for detection of specific genetic sequences using molecular beacons (MB) immobilized on solid surfaces as probes. The applicability of this miniature detect ... Full text Cite

Detection of cytochrome C in a single cell using an optical nanobiosensor.

Journal Article Analytical chemistry · May 2004 In this work, the intracellular measurement of cytochrome c using an optical nanobiosensor is demonstrated. The nanobiosensor is a unique fiberoptics-based tool which allows the minimally invasive analysis of intracellular components. Cytochrome c is a ver ... Full text Cite

Miniature biochip system for detection of Escherichia coli O157:H7 based on antibody-immobilized capillary reactors and enzyme-linked immunosorbent assay

Journal Article Analytica Chimica Acta · April 1, 2004 In this work, we report Escherichia coli O157:H7 detection using antibody-immobilized capillary reactors, enzyme-linked immunosorbent assay (ELISA), and a biochip system. ELISA selective immunological method to detect pathogenic bacteria. ELISA is also dir ... Full text Cite

Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection.

Journal Article Biosensors & bioelectronics · April 2004 We report for the first time the application of a biochip using the molecular beacon (MB) detection scheme. The usability of this biochip novel detection system for the analysis of the breast cancer gene BRCA1 is demonstrated using molecular beacon probes. ... Full text Cite

Surface-enhanced-Raman-scattering-inducing nanoprobe for spectrochemical analysis.

Journal Article Applied spectroscopy · March 2004 This paper describes a new nanoprobe that induces the surface-enhanced Raman scattering (SERS) effect when brought into contact with chemicals on any type of surface. The SERS-inducing probe was fabricated from an optical fiber that was tapered to a tip 10 ... Full text Cite

Optical sensor for the detection of caspase-9 activity in a single cell.

Journal Article Journal of the American Chemical Society · March 2004 We demonstrate for the first time, the application and utility of a unique optical sensor having a nanoprobe for monitoring the onset of the mitochondrial pathway of apoptosis in a single living cell by detecting enzymatic activities of caspase-9. Minimall ... Full text Cite

Implication of mitochondrial involvement in apoptotic activity of fragile histidine triad gene: application of synchronous luminescence spectroscopy.

Journal Article Biopolymers · March 2004 The fragile histidine triad (FHIT) tumor suppressor gene incorporates the common human chromosomal fragile site at 3p14.2. The structure and expression of the FHIT gene are frequently altered in many cancers. The tumor suppressor activity of the FHIT gene ... Full text Cite

Proceedings of SPIE - The International Society for Optical Engineering: Introduction

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2004 Cite

Detection of polycyclic aromatic compounds in single living cells using optical nanoprobes

Journal Article Polycyclic Aromatic Compounds · January 1, 2004 Exposure of mammalian cells to polycyclic aromatic compounds (PACs) such as the carcinogen benzo[a]pyrene (BaP) leads to the formation of DNA adducts N2-deoxyguanosine (dG) and N6-deoxyadenosine (dA) with adenine and guanine nucleotides, which are integral ... Full text Cite

Optical Nanosensors for Biological Applications-Spectroscopic Techniques at the Cellular Level

Journal Article · December 19, 2003 This chapter describes the basic principles of optical-based biosensors as well as discusses the evolution of the relatively new class of biosensors with nanometer dimensions and their application to the monitoring of biological species on the cellular lev ... Full text Cite

Short pulse laser propagation through tissues

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 9, 2003 An experimental and numerical study is performed to analyze short pulse laser propagation through tissue phantoms without and with inhomogeneities/tumors imbedded in it. Short pulse laser probing techniques has distinct advantages over conventional very la ... Full text Cite

Biochip using a biofluidic system for detection of E. coli and other pathogens

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 5, 2003 This work provides an overview of progress made, in our laboratory, towards the development of a practical biochip-based technology with a biofluidics system for the detection of E. coli and other pathogens. Efforts have been devoted towards efficient coup ... Full text Cite

Laser-induced fluorescence for the detection of esophageal and skin cancer

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 5, 2003 Laser-induced fluorescence (LIF) is used for in-vivo cancer diagnosis of the esophagus and skin cancer. For esophageal measurements a fiberoptic probe inserted through an endoscope was used. Autofluorescence of normal and malignant tissues were measured di ... Full text Cite

SERS gene probe for DNA diagnostics

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 5, 2003 We describe the development of a surface-enhanced Raman scattering gene (SERGen) probe technology for rapid screening for diseases and pathogens through DNA hybridization assays. The technology combines the use of gene probes labeled with SERS-active marke ... Full text Cite

Introduction

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 5, 2003 Cite

Chapter 17 Nanosensor and nanoprobe systems for in vivo bioanalysis

Journal Article Comprehensive Analytical Chemistry · December 1, 2003 Full text Cite

Proceedings of SPIE - The International Society for Optical Engineering: Introduction

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 2003 Cite

Development of a fluorescence detection system using optical parametric oscillator (OPO) laser excitation for in vivo diagnosis.

Journal Article Technology in cancer research & treatment · December 2003 In this work, the development and applications of a fluorescence detection system using optical parametric oscillator (OPO) laser excitation for in vivo disease diagnosis including oral carcinoma are described. The optical diagnosis system was based on an ... Full text Cite

Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics.

Journal Article Analytical chemistry · November 2003 The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as S ... Full text Cite

Optoacoustic diffraction tomography: analysis of algorithms.

Journal Article Journal of the Optical Society of America. A, Optics, image science, and vision · October 2003 We consider the problem of using the photoacoustic effect to image the optical properties of tissue. A region of tissue is assumed to be illuminated by frequency-modulated light that creates an ultrasonic wave of the same frequency. This wave is detected o ... Full text Cite

Monitoring intracellular proteins using fluorescence techniques: from protein synthesis and localization to activity.

Journal Article Current protein & peptide science · October 2003 The recent breakthroughs in genomics and proteomics and improvements of optical methods have made it possible to obtain localized, real-time information on intracellular proteins dynamics, through dynamic three-dimensional (3D) maps of the living cell with ... Full text Cite

Experimental and numerical analysis of short-pulse laser interaction with tissue phantoms containing inhomogeneities.

Journal Article Applied optics · September 2003 The objective is to perform an experimental and numerical study to analyze short-pulse laser propagation through tissue phantoms without and with inhomogeneities embedded in them. For a short-pulse laser the observed optical signal has a distinct temporal ... Full text Cite

Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene.

Journal Article Expert review of molecular diagnostics · September 2003 A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced ... Full text Cite

Development of a multi-spectral imaging system for medical applications

Journal Article Journal of Physics D: Applied Physics · July 21, 2003 We describe the development of a multi-spectral imaging (MSI) system based on a rapid-scanning solid-state device, an acousto-optic tunable filter (AOTF), for wavelength selection and a two-dimensional charge-coupled device for detection. The MSI device is ... Full text Cite

Short pulse laser propagation through tissues for biomedical imaging

Journal Article Journal of Physics D: Applied Physics · July 21, 2003 An experimental and numerical study is performed to analyse short pulse laser propagation through tissue phantoms with and without inhomogeneities embedded in it. Short pulse laser probing techniques have distinct advantages over conventional very large pu ... Full text Cite

Advanced biochip: Principle and applications in medical diagnostics and pathogen detection

Journal Article Proceedings - IEEE International Symposium on Circuits and Systems · July 14, 2003 An important factor in medical diagnostics is rapid, selective, and sensitive detection of biochemical substances (proteins, metabolites, nucleic acids), biological species or living systems (bacteria, virus or related components) at trace levels in biolog ... Cite

Application of an integrated microchip system with capillary array electrophoresis to optimization of enzymatic reactions

Journal Article Analytica Chimica Acta · July 1, 2003 In this work, a combination of complementary metal-oxide semiconductor (CMOS) microchip system with capillary array electrophoresis (CAE) is demonstrated as a system for optimizing conditions for enzymatic reaction. Dimethylacridinone (DDAO)-phosphate subs ... Full text Cite

Critical assessment: Use of supersonic jet spectrometry for complex mixture analysis (IUPAC Technical Report)

Journal Article Pure and Applied Chemistry · July 1, 2003 When cooled to a temperature of a few K using supersonic jet expansion into a vacuum, a molecule exists in the lowest vibrational level of the ground electronic state and is isolated at collision-free conditions. The absorption or excitation/fluorescence s ... Full text Cite

Photoacoustic method for the simultaneous acquisition of optical and ultrasonic spectra

Journal Article Acoustic Research Letters Online · May 30, 2003 A novel method for the simultaneous acquisition of optical absorption and ultrasonic attenuation spectra is described. The technique is based on the photoacoustic generation of bi-directional ultrasonic pulses by a thin optical absorber that is remote from ... Full text Cite

Photochemically prepared gold nanoparticles: A substrate for surface enhanced Raman scattering

Journal Article Current Science · May 25, 2003 Gold nanoparticles have been prepared in aqueous Triton X-100 medium using a new photochemical procedure. The characterization of the particle is made using transmission electron microscopy studies. The effects of analyte molecules such as pyridine and sal ... Cite

Multi-functional biochip for medical diagnostics and pathogen detection

Journal Article Sensors and Actuators, B: Chemical · April 20, 2003 We describe a multi-functional biochip (MFB), which uses two different types of bioreceptors, including nucleic acid and antibody probes, on a single platform. The multi-functional capability of the MFB device for biomedical diagnostics is illustrated by m ... Full text Cite

Multi-functional biochip for biological agent detection

Conference 2003 International Semiconductor Device Research Symposium, ISDRS 2003 - Proceedings · January 1, 2003 There is an increasing need to develop rapid, simple, cost-effective sensor devices for screening multiple pathogens for homeland defense applications. With its multifunctional capability, the multifunctional biochip (MFB) is a unique system that allows si ... Full text Cite

Real-time detection of PAH mixtures in the vapor phase at high temperatures

Conference Journal of Analytical and Applied Pyrolysis · January 1, 2003 Laser-induced fluorescence (LIF) spectroscopy can serve as a rapid analytical method for the quantitative analysis of polycyclic aromatic hydrocarbons (PAHs). The high quantum yields of this class of compounds, along with large absorption cross-sections, m ... Full text Cite

Amplification techniques for optical detection

Chapter · January 1, 2003 This chapter presents a summary account of various amplification techniques for analysis of biological molecules (species) that use some form of optical detection as the endpoint. The analyte species possibly under consideration include lipids, carbohydrat ... Cite

Theoretical models and algorithms in optical diffusion tomography

Chapter · January 1, 2003 The potential of optical tomography as a new diagnostic tool has stimulated considerable interest in the last 10 years. Although limited to about the first 5 cm of the body, the technique offers several advantages often not available in established imaging ... Cite

Preface

Book · January 1, 2003 Cite

Living-cell analysis using optical methods

Chapter · January 1, 2003 Previous chapters in this handbook have extensively described new methods in the field of biophotonics and reviewed applications. The scope of this chapter is to present, from the biologist’s point of view, a synthesis of the current and potential applicat ... Cite

Biochips and microarrays: Tools for the new medicine

Chapter · January 1, 2003 Biochips and microarrays are technologies that will benefit directly from the research advances of the postsequencing era in genomics. Experimental genomics, in combination with the growing body of sequence information, promises to revolutionize the way ce ... Cite

Biomedical photonics: Handbook

Book · January 1, 2003 A wide variety of biomedical photonic technologies have been developed recently for clinical monitoring of early disease states; molecular diagnostics and imaging of physiological parameters; molecular and genetic biomarkers; and detection of the presence ... Cite

Biomedical photonics: A revolution at the interface of science and technology

Chapter · January 1, 2003 Throughout human history, light has played an important role in medicine. In prehistoric times, the healing power of light was often attributed to mythological, religious, and supernatural powers. The history of light therapy dates back to the ancient Egyp ... Cite

Fluorescence spectroscopy for biomedical diagnostics

Chapter · January 1, 2003 In the past several decades, fluorescence spectroscopy has had a dramatic effect on many different fields of research. One field that has seen significant advancements is biomedical diagnostics. Within this field, fluorescence spectroscopy has been applied ... Cite

Basic instrumentation in photonics

Chapter · January 1, 2003 This chapter presents an overview of the basic instrumentation used in photonic applications. The chapter is intended to provide an introduction to the basic setups, apparatus, and system components for readers from other research fields who wish to become ... Cite

Biosensors for medical applications

Chapter · January 1, 2003 Human beings, along with other mammals, consciously interact with the surrounding world by means of seven sensing mechanisms. In addition to the five senses, the abilities to detect temperature and variations in elevation are almost as important. One canno ... Cite

Optical properties of tissue

Chapter · January 1, 2003 The electromagnetic spectrum provides a diverse set of photonic tools for probing, manipulating, and interacting with biological systems. A great variety of electromagnetic phenomena are used in biomedicine to detect and treat disease and to advance scient ... Cite

Nanosensors for single-cell analyses

Chapter · January 1, 2003 Over the years, new techniques in chemical and biological sensing have set the stage for great advances in the field of biological research. One of the most recent technological advances in this area has been the development of nanosensors. A nanosensor is ... Cite

Spectroscopic data of biologically and medically relevant species and samples

Chapter · January 1, 2003 This chapter provides several tables summarizing spectroscopic properties of various biologically and medically relevant species and samples. It is divided into two main sections: (1) Fundamental Spectroscopic Properties of Biologically and Medically Relev ... Cite

Surface-enhanced raman scattering (SERS) for biomedical diagnostics

Chapter · January 1, 2003 Raman spectroscopy is based on vibrational transitions that yield very narrow spectral features characteristic of the investigated sample. Thus, it has long been regarded as a valuable tool for the identification of chemical and biological samples as well ... Cite

Detection of bacterial pathogen DNA using an integrated complementary metal oxide semiconductor microchip system with capillary array electrophoresis.

Journal Article Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · January 2003 In this paper, we show an integrated complementary metal oxide semiconductor (CMOS)-based microchip system with capillary array electrophoresis (CAE) for the detection of bacterial pathogen amplified by polymerase chain reaction (PCR). In order to demonstr ... Full text Cite

Multi-analyte analysis system using an antibody-based biochip.

Journal Article Analytical and bioanalytical chemistry · January 2003 A multi-analyte detection system using a unique antibody (Ab) biochip is described. The Ab-based biochip, also referred to as the protein biochip, uses a sensor array based on a complementary metal oxide silicon (CMOS) integrated circuit. The Ab-biochip ha ... Full text Cite

Real-time detection of PAH mixtures in the vapor phase at high temperatures

Journal Article Journal of Analytical and Applied Pyrolysis · 2003 Laser-induced fluorescence (LIF) spectroscopy can serve as a rapid analytical method for the quantitative analysis of polycyclic aromatic hydrocarbons (PAHs). The high quantum yields of this class of compounds, along with large absorption cross-sections, m ... Cite

A miniature biochip system for detection of aerosolized Bacillus globigii spores.

Journal Article Analytical chemistry · January 2003 The feasibility of using a novel detection scheme for the analysis of biological warfare agents is demonstrated using Bacillus globigii spores, a surrogate species for Bacillus anthracis. In this paper, a sensitive and selective enzyme-linked immunosorbent ... Full text Cite

Real-time monitoring of polycyclic aromatic hydrocarbons in cigarette smoke using time-resolved laser-induced fluorescence

Journal Article Polycyclic Aromatic Compounds · January 1, 2003 Cigarette smoke contains a large number of chemicals and gaseous compounds including polycyclic aromatic hydrocarbons (PAHs). Due to the importance of PAHs and their health effects in cigarettes, development of systems to analyze PAHs in cigarette smoke ma ... Full text Cite

Crossed-beam two-photon readout system for three-dimensional radiation dosimeters

Journal Article Review of Scientific Instruments · December 1, 2002 A two-photon readout system, that allowed for the readout of information on ionizing radiation from the three dimensional (3D) optical random access memory (ORAM) materials without polymer fracturing, was investigated. A Q-switched, frequency-doubled Nd:YA ... Full text Cite

Nanosensor for in vivo measurement of the carcinogen benzo[a]pyrene in a single cell.

Journal Article Journal of nanoscience and nanotechnology · December 2002 This work describes the fabrication and the application of an antibody-based fiber-optic nanosensor for in situ measurements of the carcinogen benzo[a]pyrene (BaP) in a single cell. This antibody-based spectroscopic nanosensor is miniaturized enabling the ... Full text Cite

Surface-enhanced Raman scattering detection of the breast cancer susceptibility gene BRCA1 using a silver-coated microarray platform

Journal Article Analytica Chimica Acta · September 26, 2002 Surface-enhanced Raman scattering (SERS) spectroscopy was used to monitor DNA hybridization of a fragment of the BRCA1 breast cancer susceptibility gene on modified silver surfaces. Rhodamine B was covalently attached to a 5′-amino-labeled oligonucleotide ... Full text Cite

Integrated circuit microchip system with multiplex capillary electrophoresis module for DNA analysis

Journal Article Analytica Chimica Acta · August 27, 2002 In this paper, we describe the use of an integrated circuit (IC) microchip system as a detector in multiplex capillary electrophoresis (CE). This combination of multiplex capillary gel electrophoresis and the IC microchip technology represents a novel appr ... Full text Cite

Integrated CMOS microchip system with capillary array electrophoresis.

Journal Article Analytical and bioanalytical chemistry · July 2002 A complementary metal oxide semiconductor (CMOS)-capillary array electrophoresis (CAE) system has been used for DNA analysis. Because of its compactness and multiplex capability, the CAE-CMOS microchip is very suitable for the construction of a miniaturize ... Full text Cite

Novel modeling techniques for tumor diagnostics using pulsed laser sources

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · May 13, 2002 In this paper, a two-dimensional transient radiation transport algorithm is developed to analyze short pulse laser transport through a tissue medium having tumors and inhomogeneities imbedded in it. Short pulse probing techniques have distinct advantages o ... Full text Cite

Characterization of antibodies against benzo[a]pyrene with thermodynamic and kinetic constants.

Journal Article Talanta · April 2002 Antibodies of a polyclonal antiserum against benzo[a]pyrene were characterized by determining thermodynamic and kinetic constants of the antigen-antibody reaction. Label-free binding assays with optical detection based on reflectometric interference spectr ... Full text Cite

Analysis of short-pulse laser photon transport through tissues for optical tomography.

Journal Article Optics letters · March 2002 We describe a method for analyzing short-pulse laser propagation through tissues for the detection of tumors and inhomogeneities in tissues with the goal of developing a time-resolved optical tomography system. Traditional methods for analyzing photon tran ... Full text Cite

Investigation of Lysozyme-Chitobioside Interactions Using Synchronous Luminescence and Lifetime Measurements

Journal Article Journal of Fluorescence · March 1, 2002 Beside their ability to disrupt the outer membranes of some microorganisms, lysozymes also experience interactions with chitins or their fluorescent analogs. It has been well established that chitins bind to the cleft of lysozymes and the subsites of the l ... Full text Cite

Radiation dosimetry using three-dimensional optical random access memories

Journal Article SOLID STATE DOSIMETRY, PTS 1 AND 2, PROCEEDINGS · 2002 Cite

Development of a portable Raman spectrometer for medical diagnostics

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2002 This paper describes a self-contained, portable Raman instrument that has been developed for biomedical analyses. The instrument consists of a 785-nm diode laser for excitation, an acousto-optic tunable filter (AOTF) for wavelength discrimination, and an a ... Full text Cite

In vivo fluorescence spectroscopy for diagnosis of skin cancer

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2002 Laser-induced fluorescence spectroscopy at the excitation of 410 nm and using the fluorescence intensity as a marker was used as an in vivo technique for detection of squamous cell carcinoma, basal cell carcinoma and precancerous lesions. The technique wor ... Full text Cite

Transcranial ultrasound for brain injury monitoring: Initial clinical studies

Journal Article Proceedings of SPIE-The International Society for Optical Engineering · January 1, 2002 In this work, we discuss data acquired in a clinical investigation to evaluate the utility of transcranial ultrasound for monitoring/detecting brain injury. Using a portable ultrasonic data acquisition system, over one thousand transcranial waveforms were ... Full text Cite

Multi-spectral imaging for medical diagnostics

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2002 We report the development of a multi-spectral imaging (MSI) device for medical diagnostic applications. The MSI device uses an acousto-optic tunable filter (AOTF) for wavelength selection and a two-dimensional charge-coupled device (CCD) for detection. Unl ... Full text Cite

Opto-ultrasonic system for generation of ultrasound and optical detection

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2002 In this work, we examine a system that exploits the photoacoustic effect to act as an ultrasonic pulse generator and an optical detector. At the core of the system are a solid substrate and an optical absorber. To test the performance as an ultrasonic gene ... Full text Cite

Proceedings of SPIE: Biomedical Diagnostic, Guidance, and Surgical-Assist Systems IV: Introduction

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2002 Cite

Nanosensors: Design and application to site-specific cellular analyses

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2002 We report the development and application of an antibody-based nanoprobe for in situ measurements within a single cell. The nanoprobe has an antibody-based probe targeted to benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyrene (BaP) and ... Full text Cite

Simultaneous detection of the tumor suppressor FHIT gene and protein using the multi-functional biochip.

Journal Article Cancer detection and prevention · January 2002 The tumor suppressor gene, fragile histidine triad (FHIT), encompasses the most common human chromosomal fragile site, at 3pl4.2. Detection of FHIT gene is important in cancer diagnostics since its alterations have been associated with several human cancer ... Full text Cite

Nanobiosensors: probing the sanctuary of individual living cells.

Journal Article Journal of cellular biochemistry. Supplement · January 2002 Recently, nanotechnology has been revolutionizing important areas in molecular biology, especially diagnostics and therapy at the molecular and cellular level. The combination of nanotechnology, biology, and photonics opens the possibility of detecting and ... Full text Cite

Laser-induced fluorescence spectroscopy for in vivo diagnosis of non-melanoma skin cancers.

Journal Article Lasers in surgery and medicine · January 2002 Background and objectivesLaser-induced fluorescence spectroscopy is a non-invasive technique previously used for detection of cancer in a variety of organ systems. The objective of this study was to determine whether in vivo laser-induced fluoresc ... Full text Cite

Radiation dosimetry using three-dimensional optical random access memories.

Journal Article Radiation protection dosimetry · January 2002 The ability to determine particle type and energy plays an important role in the dosimetry of heavy charged particles (HCP) and neutrons. A new approach to radiation dosimetry is presented, which is shown to be capable of particle type and energy discrimin ... Full text Cite

Cancer gene detection using surface-enhanced Raman scattering (SERS)

Journal Article Journal of Raman Spectroscopy · January 1, 2002 We describe the development of a surface-enhanced Raman scattering (SERS) method for cancer gene detection. The technology uses DNA gene probes based on SERS labels for gene detection. The detection method uses nanostructured metallic substrates as SERS-ac ... Full text Cite

Micro arrays and biochips: Applications and potential in genomics and proteomics

Journal Article Current Genomics · December 13, 2001 This report provides an overview of the development and applications of DNA-based microarrays and biochip technology in genomics. DNA microarrays and biochip technologies are having a significant impact on a wide variety of areas in genomic research. Many ... Full text Cite

High-temperature vapor detection of polycyclic aromatic hydrocarbon fluorescence

Journal Article Fuel · October 1, 2001 Polycyclic aromatic hydrocarbons (PAHs) are generated from the incomplete combustion of organic matters in a wide variety of natural and man-made materials, such as fuels. It is important to develop an analytical technique for rapidly monitoring PAH genera ... Full text Cite

Microarray sampling-platform fabrication using bubble-jet technology for a biochip system.

Journal Article Fresenius' journal of analytical chemistry · September 2001 The fabrication of microarrays containing PCR-amplified genomic DNA extracts from mice tumors on a Zetaprobe membrane using a modified thermal ink-jet printer is described. A simple and cost-effective procedure for the fabrication of microarrays containing ... Full text Cite

Laser-induced fluorescence studies of polycyclic aromatic hydrocarbons (PAH) vapors at high temperatures.

Journal Article Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · June 2001 In this work, we present the fluorescence spectra of anthracene and pyrene vapors at different elevated temperatures (from 150 to 650 degrees C) excited with the 337 nm line of a nitrogen laser. We describe the high temperature effects on the resulting spe ... Full text Cite

Application of an antibody biochip for p53 detection and cancer diagnosis.

Journal Article Biotechnology progress · May 2001 Detection of the p53 tumor suppressor gene is important in early cancer diagnostics because alterations in the gene have been associated with carcinogenic manifestations in several tissue types in humans. We have developed an antibody-based detection instr ... Full text Cite

Nanosensors and biochips: Frontiers in biomolecular diagnostics

Journal Article Sensors and Actuators, B: Chemical · April 15, 2001 In the past two decades, the biotechnology and medical fields have seen great advances in the development of novel technologies that open new horizons for identifying and quantifying biomolecules, and diagnosing diseases. This manuscript provides an overvi ... Full text Cite

Biomedical implications of protein folding and misfolding.

Journal Article Biotechnology and applied biochemistry · February 2001 A review of protein-folding mechanisms is presented to indicate (i) the formation of correctly folded and misfolded forms, and (ii) the biomedical implications involved. Protein mechanisms may be classified into series and series-parallel mechanisms. The m ... Full text Cite

Detection of E. coli using a microfluidics-based antibody biochip detection system.

Journal Article Fresenius' journal of analytical chemistry · February 2001 This work demonstrates the detection of E. coli using a 2-dimensional photosensor array biochip which is efficiently equipped with a microfluidics sample/reagent delivery system for on-chip monitoring of bioassays. The biochip features a 4 x 4 array of ind ... Full text Cite

A kinetic analysis using fractals of cellular analyte-receptor binding and dissociation.

Journal Article Biotechnology and applied biochemistry · February 2001 A fractal analysis is presented for cellular analyte-receptor binding and dissociation kinetics using a biosensor. Data taken from the literature may be modelled, in the case of binding, using a single-fractal analysis or a dual-fractal analysis. The dual- ... Full text Cite

Proceedings of SPIE - The International Society for Optical Engineering: Introduction

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 Cite

Vapor detection of polyaromatic compounds using laser-induced fluorescence

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 In this work, we present the fluorescence spectra of anthracene and pyrene vapors at different elevated temperatures (from 150 to 650°C) excited with the 337 nm line of a nitrogen laser. We describe the high temperature effects on the resulting spectral pr ... Full text Cite

Laser-induced fluorescence spectroscopy for skin cancer diagnostics

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 Objective: The goal of this pilot study was to determine whether in vivo laser-induced fluorescence intensity may be used to distinguish normal skin from malignant lesions. Materials and Methods: The system consisted of a nitrogen/dye laser tuned at 410 nm ... Full text Cite

Ultrasonic scattering and transmission properties of mammalian white matter in the detection of brain injury

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 In this work, we examine the orientation-dependent scattering and attenuation properties of white matter from mammalian brain tissue. We find that both the backscatter and attenuation of ultrasound in these tissues exhibit anisotropy. Furthermore, when ext ... Full text Cite

Field-portable AOTF-based monitor technology for environmental sensing

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 The development of a field-portable Raman instrument for environmental analyses is described. It is based on the use of a near-infrared frequency-stabilized diode laser for excitation, an acousto-optic tunable filter for wavelength selection, and an avalan ... Full text Cite

Ultrasonic diffraction in the design of photoacoustic probes

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 In this work, we examine the role of diffraction in the design of ring-shaped ultrasonic receivers for photoacoustic probes. The sensitivity patterns of ultrasonic receiving rings are calculated at 1 MHz using the angular spectrum technique. Three separate ... Full text Cite

Detection of neutrons using a novel three-dimensional optical random access memory technology (3D-ORAM)

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 We report for the first time, the development of a three-dimensional optical random access memory (3D-ORAM) material for neutron dosimetry. Detection of energetic neutrons is important in many applications, spanning from radioactive waste monitoring to spa ... Full text Cite

Field-portable AOTF-based monitor technology for environmental sensing

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 The development of a field-portable Raman instrument for environmental analyses is described. It is based on the use of a near-infrared frequency-stabilized diode laser for excitation, an acousto-optic tunable filter for wavelength selection, and an avalan ... Full text Cite

Use of short-pulse laser for optical tomography of tissues

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 This paper analyzes the short pulse laser propagation through tissues for development of a time-resolved optical tomography system for detection of tumors and inhomogeneities in tissues. Traditional method for analyzing optical transport in tissues is the ... Full text Cite

Nanosensors for the analysis of a single cell

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 Submicron fiber-optic biosensors have been developed and used to measure toxic chemicals within single cells. These sensors are fabricated by pulling the distal-end of an optical fiber to a diameter of less than one micron and coating them with antibodies ... Full text Cite

Nanosensors for biochemical analysis of single cells

Journal Article Chimica Oggi · January 1, 2001 In the past couple of decades, new techniques in chemical and biological sensing have provided information and technology that have greatly enhanced the field of biological research. In this area of sensing, one of the most recent technological advances ha ... Cite

Synchronous luminescence: A simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein

Journal Article Biotechnology Letters · January 1, 2001 Human fragile histidine triad (FHIT) protein has dinucleoside 5′,5‴-P1, Pn-polyphosphates hydrolysis activity, with AMP being one of the reaction products. Application of synchronous luminescence (SL) spectroscopy, in which both excitation and emission wav ... Full text Cite

Proceedings of SPIE - The International Society for Optical Engineering: Introduction

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2001 Cite

Detection of E. coli using a microfluidics-based antibody biochip detection system

Journal Article Fresenius' Journal of Analytical Chemistry · 2001 This work demonstrates the detection of E. coli using a 2-dimensional photosensor array biochip which is efficiently equipped with a microfluidics sample/reagent delivery system for on-chip monitoring of bioassays. The biochip features a 4 × 4 array of ind ... Cite

Surface-enhanced Raman of dopamine and neurotransmitters using sol-gel substrates and polymer-coated fiber-optic probes

Journal Article Applied Spectroscopy · December 1, 2000 Surface-enhanced Raman spectroscopy (SERS) is used to detect catecholamines - viz., dopamine, norepinephrine, epinine, and isoproterenol - using a new substrate prepared with sol-gel technology. Catecholamines are complexed at the surface by iron(III) ions ... Full text Cite

Native fluorescence and Mag-indo-1 -protein interaction as tools for probing unfolding and refolding sequences of the bovine serum albumin subdomain in the presence of guanidine hydrochloride

Journal Article Protein Journal · December 1, 2000 Changes in the fluorescence spectrum of tryptophans Trp 134 and Trp 212 in bovine serum albumin (BSA) and of Trp 214 of human serum albumin in the presence of the chaotropic agent guanidine hydrochloride (Gnd) were studied. A detailed analysis of the fluor ... Cite

Three-dimensional optical random access memory materials for use as radiation dosimeters.

Journal Article Analytical chemistry · November 2000 This article describes the development of the first three-dimensional optical random access memory (3D-ORAM) material and readout system for monitoring energetic neutrons. Two different photochromic dyes, 5'-chloro-6-nitro-1',3',3'-trimethylspiro-[2H-1-ben ... Full text Cite

Development of an integrated single-fiber SERS sensor

Journal Article Sensors and Actuators, B: Chemical · September 10, 2000 An integrated fiberoptic surface-enhanced Raman scattering (SERS) sensor based on a single-fiber system is described and evaluated for remote and in situ measurements. The heart of the sensor consists of a unique single optical fiber designed to induce the ... Full text Cite

The development of optical nanosensors for biological measurements.

Journal Article Trends in biotechnology · September 2000 This article discusses and documents the basic concepts of, and developments in, the field of optical nanosensors and nanobiosensors. It describes the progression of this field of research from its birth up to the present, with emphasis on the techniques o ... Full text Cite

Native fluorescence and mag-indo-1-protein interaction as tools for probing unfolding and refolding sequences of the bovine serum albumin subdomain in the presence of guanidine hydrochloride.

Journal Article Journal of protein chemistry · August 2000 Changes in the fluorescence spectrum of tryptophans Trp 134 and Trp 212 in bovine serum albumin (BSA) and of Trp 214 of human serum albumin in the presence of the chaotropic agent guanidine hydrochloride (Gnd) were studied. A detailed analysis of the fluor ... Full text Cite

Antibody-based biosensor for breast cancer with ultrasonic regeneration.

Journal Article Journal of biomedical optics · July 2000 We describe a novel method and instrumental setup for regenerating antibodies immobilized on a fiberoptic probe of an immunosensor using ultrasonic irradiation with broadband imaging transducers. The instrumental setup and irradiation conditions for antibo ... Full text Cite

Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell.

Journal Article Nature biotechnology · July 2000 We report here the application of an antibody-based nanoprobe for in situ measurements of a single cell. The nanoprobe employs antibody-based receptors targeted to a fluorescent analyte, benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyre ... Full text Cite

Biosensors and biochips: advances in biological and medical diagnostics.

Journal Article Fresenius' journal of analytical chemistry · March 2000 In the past two decades, the biological and medical fields have seen great advances in the development of biosensors and biochips capable of characterizing and quantifying biomolecules. This review is meant to provide an overview of the various types of bi ... Full text Cite

Bio-imaging system using an acousto-optic tunable filter

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2000 The interaction of light with tissue has been used to recognize disease since the mid-1800s. The recent developments of light sources, detectors, and fiber optic probes provide opportunities to measure these interactions, which yield information for tissue ... Cite

Principle of synchronous luminescence (SL) technique for biomedical diagnostics

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2000 In the present study, the principle of synchronous luminescence (SL) is described for use in biomedical diagnostics. The SL method involves scanning simultaneously both emission and excitation wavelengths while keeping a constant wavelength interval betwee ... Cite

Method for the simultaneous acquisition of optical absorption and sonic attenuation with photoacoustic ultrasound

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2000 In this work, we present a novel method for the simultaneous acquisition of ultrasonic attenuation and optical absorption using photoacoustically-generated ultrasound. We discuss the theory behind the technique and apply the method to measurements in mamma ... Cite

Development of nanosensors and bioprobes

Journal Article Journal of Nanoparticle Research · January 1, 2000 We describe the development and application of nanosensors having bioreceptor probes for bioanalysis. The nanoprobes were fabricated with optical fibers pulled down to tips having distal end sizes of approximately 30-60 nm. The use of two different types o ... Full text Cite

ASSTC and field sensors: new technology for emergency care

Journal Article Proc. SPIE - Int. Soc. Opt. Eng. (USA) · 2000 The US Army Medical Research and Material Command together with the US Marine Corps Combat Development Command sponsored the design and production of a far-forward, lightweight, small footprint, reconfigurable, highly mobile Advanced Surgical Suite for Tra ... Full text Link to item Cite

Development of a compact, handheld Raman instrument with no moving parts for use in field analysis

Journal Article Review of Scientific Instruments · January 1, 2000 This article describes a lightweight, self-contained, field portable Raman instrument that has been developed for rapid on-site determination of primary mixture components. The instrument consists of a helium neon (HeNe) laser for excitation, an acousto-op ... Full text Cite

Instrumentation and measurement issues for nanometer particles: Workshop summary

Journal Article Journal of Nanoparticle Research · January 1, 2000 Full text Cite

High-temperature fluorescence measurements and instrumentation for polyaromatic hydrocarbons (PAH): A review

Journal Article Polycyclic Aromatic Compounds · January 1, 2000 This paper provides an overview of high-temperature measurements of polyaromatic hydrocarbons (PAHs) and an assessment of the state-of the art methods and instrumentation that are used for those measurements. This review deals specifically with the fluores ... Full text Cite

Intracellular measurements in mammary carcinoma cells using fiber-optic nanosensors.

Journal Article Analytical biochemistry · January 2000 Submicrometer fiber-optic biosensors have been developed and used to measure toxic chemicals within single cells. Optical fibers that have been pulled to a distal-end diameter of less than 1 micrometer are coated with antibodies to selectively bind the spe ... Full text Cite

Optical nanosensors and biological measurements

Journal Article Biofutur · January 1, 2000 Full text Cite

Laser-induced fluorescence studies of polyaromatic hydrocarbons (PAH) vapors at high temperatures

Journal Article · January 1, 2000 A study on how temperature changes affect the fluorescence properties of PAH, such as fluorescence intensity, bandwidth, etc., was carried out using an instrumental system. Light from a nitrogen laser, 337 nm, was utilized to excite anthracene and pyrene. ... Cite

Near infrared (NIR) emitter/detector probe for sensing buried objects and landmines

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1999 The detection of landmines and buried objects requires methods that can cover large areas rapidly while providing the required sensitivity to detect the optical and spectroscopic contrast in soil properties that can reveal their presence. These conditions ... Cite

Development of instrumental ORAM system for radiation dosimetry

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1999 The development of an optical-based dosimeter for neutrons and heavy charged particles is described. It is based on the use of three dimensional (3-D) optical memory materials, used in optical computing applications, and multiphoton fluorescence of photoch ... Cite

Demonstration of a separations-based fiberoptic sensor for bioanalysis

Journal Article Analytica Chimica Acta · November 15, 1999 We present a newly-developed separations-based fiberoptic sensor (SBFOS) for biomedical applications. The sensor features a linear 10 cm effective length section of capillary coupled with a single optical fiber for laser- induced fluorescence (LIF) detecti ... Full text Cite

Evaluation of a chemical vapor dosimeter using polymer-coated SERS substrates

Journal Article Analytica Chimica Acta · November 15, 1999 This paper describes the development and evaluation of an improved passive chemical dosimeter using poly(vinylpyrrolidone) coating on a surface- enhanced Raman scattering (SERS) substrate for monitoring airborne chemicals. Experimental studies are performe ... Full text Cite

Photoacoustic measurements in brain tissue

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1999 In this work, we develop and evaluate the photoacoustic technique for recording spectra of white and gray mammalian brain tissues. In additional to the experimental work, we also discuss the geometric aspects of photoacoustic signal generation using collim ... Cite

Surface-enhanced Raman scattering (SERS) method and instrumentation for genomics and biomedical analysis

Journal Article Journal of Raman Spectroscopy · January 1, 1999 The development of a surface-enhanced Raman scattering (SERS) method and instrument for use in biomedical and genomics analysis is described. The technology uses DNA gene probes based on SERS labels for gene detection and DNA mapping. The detection method ... Full text Cite

Signal expressions and initial design for a 3-D ORAM radiation dosimeter reader

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1999 Three-dimensional optical memories which utilize two-photon processes have been proposed for use as a radiation dosimeter. The 'bits' stored by the optical memory are altered by the passage of radiation and can yield information about the dose and energy. ... Cite

Subwavelength Raman imaging of biological samples using near-field spectroscopy

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1999 Scanning near-field optical microscopy (SNOM) and Raman scattering were combined to obtain subwavelength molecular resolution. Near-field microscopy allows to overcome the diffraction limit valid for all lens or mirror based optical instruments and lateral ... Cite

DNA biochip using a phototransistor integrated circuit.

Journal Article Analytical chemistry · January 1999 This work describes the development of an integrated biosensor based on phototransistor integrated circuits (IC) for use in medical detection, DNA diagnostics, and gene mapping. The evaluation of various system components developed for an integrated biosen ... Full text Cite

AOTF-based remote sensor with sol-gel probe

Journal Article Instrumentation Science and Technology · January 1, 1999 We report the development and application of a sensor using acousto-optic tunable filter (AOTF) and sol-gel probe technology. A pH-sensitive probe is used as a model sensing system with dextran derivatives of pH sensitive dyes doped into sol-gel thin films ... Full text Cite

Mag-indo-1-protein interaction as a tool for probing the 3D conformation of protein sub-domains: Influence of the chemical microenvironment of the histidin residue(s) on the parameters of the interaction

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1999 Magnesium complexation with Mag-indo-1 results in a shift of the emission fluorescence spectrum from 480 nm to 417 nm. Mag-indo-1 is also able to bind calcium and zinc. These cationic interactions induce the same spectral shift but the fluorescence intensi ... Cite

Monitoring system for the study of autotrophic biofilms in bioremediation of polyaromatic compounds

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1999 A portable laser-induced fluorescence device was developed for detecting polyaromatic hydrocarbons (PAHs). The device was tested in the laboratory by using anthracene and pyrene PAHs model systems. These PAHs were successfully field screened in surface wat ... Cite

A new surface-enhanced Raman scattering substrate based on silver nanoparticles in sol-gel

Journal Article Journal of Raman Spectroscopy · January 1, 1999 We report for the first time the development of a new surface-enhanced Raman scattering (SERS)-active silver chloride silica-glass substrate prepared by using in situ precipitation of silver chloride particles in sol-gel films as a precursor for nanopartic ... Full text Cite

Hyperspectral imaging using AOTF and NIR sensing of buried objects and landmines

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1999 The detection of landmines and buried objects requires methods that can cover large areas rapidly while providing the required sensitivity to detect the optical and spectroscopic contrasts in soil properties that can reveal their presence. These conditions ... Cite

Application of ultrasonic techniques for brain injury diagnosis

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1999 In this work, we evaluate methods for detecting brain injury using ultrasound. We have used simulations of ultrasonic fields in the head to model the phase distortion of the skull. In addition we present experimental data from the crania of large animals. ... Cite

Fiber optics SERS sensors for environmental monitoring

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1999 The development of fiberoptic sensors for remote in-situ environmental monitoring using surface-enhanced Raman scattering (SERS) is described. The approach to sensor development includes the initial development of SERS-active media on nanoparticle-based so ... Cite

Vibrational spectrum of strychnine: Detection at the nanogram level using a Raman microscope

Journal Article Journal of Raman Spectroscopy · January 1, 1999 The complete vibrational spectra of strychnine is reported. Strychnine, a compound with medicinal value at the proper dosage, is also a potent toxin at certain concentration levels. Down to 5 ng of strychnine were detected from its spectrum using a microsc ... Full text Cite

Surface-enhanced Raman detection of chemical vapors with the use of personal dosimeters

Journal Article Field Analytical Chemistry and Technology · January 1, 1999 This article provides an overview of the development and application of surface-enhanced Raman scattering (SERS) for the detection of vapors of organic chemicals with the use of personal dosimetry. The approach used with this personal dosimeter requires no ... Full text Cite

Differentiation of normal and neoplastic cells by synchronous fluorescence: Rat liver epithelial and rat hepatoma cell models

Journal Article Analytical Letters · January 1, 1999 In the present study, conventional and synchronous luminescence (SL) were utilized to investigate spectral differences in normal and neoplastic cells. The synchronous fluorescence (SE) method involves scanning simultaneously both emission and excitation wa ... Full text Cite

Development of a DNA biochip for gene diagnosis

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1998 We describe a biochip based on an integrated circuit photodiode array for use in medical diagnostics. The biochip is a self-contained device which has photosensors, amplifiers, discriminators and logic circuitry on board. The development and evaluation of ... Full text Cite

SERODS: A new medium for high-density optical data storage

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1998 A new optical data storage technology based on the surface-enhanced Raman scattering (SERS) effect has been developed for high-density optical memory and three-dimensional data storage. With the surface-enhanced Raman optical data storage (SERODS) technolo ... Full text Cite

Mag-indo-1 as a potential reporter of the 3D conformation of protein sub-domains

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1998 Mag-indo-1 is a well known fluorescent probe. Magnesium complexation results in a shift of the emission fluorescence spectrum from 480 nm to 417 nm with an intensity proportional to the magnesium concentration in the range 0.6 to 30 mM. Although designed a ... Full text Cite

Evaluation of ultrasound techniques for brain injury detection

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1998 In this work we examine the physics underlying wave propagation in the head to evaluate various ultrasonic transducers for use in a brain injury detection device. The results of measurements of the attenuation coefficient and phase velocity for ultrasonic ... Full text Cite

Some approaches to infrared spectroscopy for detection of buried objects

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1998 Detection of buried objects presents a formidable challenge which requires many different approaches. Infrared imaging has proven its versatility in a number of applications. Recent advances in technology have opened the door for spectroscopic imaging syst ... Full text Cite

A new fluorescence method for the determination of ammonia

Journal Article Indian Journal of Chemistry - Section A Inorganic, Physical, Theoretical and Analytical Chemistry · December 1, 1998 A new method is described for the determination of ammonia by exploiting the change in fluorescence intensity of the ethanolic solution of salicylaldehyde with the addition of trace amounts of aqueous ammonia solution. The method is simple, sensitive and a ... Cite

Erratum: (Proceedings of Biomedical Sensing and Imaging Technologies (26-27 January 1998))

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1998 Cite

Proceedings of SPIE - The International Society for Optical Engineering: Introduction

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1998 Cite

Monitoring and characterization of polyaromatic compounds in the environment.

Journal Article Talanta · November 1998 This paper provides an overview of analytical techniques and instruments used to monitor and characterize polycyclic aromatic compounds (PACs) in the environment. The basic operating principles of various analytical approaches and systems are presented. Th ... Full text Cite

Rapid screening method for cocaine and benzoylecgonine in saliva samples

Journal Article Analytica Chimica Acta · October 26, 1998 We are presenting a new method for cocaine and benzoylecgonine analysis in saliva samples based on room temperature phosphorimetry. The determination of the drugs is performed on filter paper, which is a suitable substrate for most saliva collection proced ... Full text Cite

Single- and dual-fractal analysis of hybridization binding kinetics: biosensor applications.

Journal Article Biotechnology progress · September 1998 The diffusion-limited hybridization kinetics of analyte in solution to a receptor immobilized on a biosensor or immunosensor surface is analyzed within a fractal framework. The data may be analyzed by a single- or a dual-fractal analysis. This was indicate ... Full text Cite

Development of a DNA biochip: Principle and applications

Journal Article Sensors and Actuators, B: Chemical · August 31, 1998 This paper provides an overview of the operating principle of a DNA biochip based on integrated circuit for use in biomedical diagnostics. The device is a self-contained system with photosensors, amplifiers, discriminators and logic circuitry on board. The ... Full text Cite

Surface-enhanced Raman spectroscopy using metallic nanostructures

Journal Article TrAC - Trends in Analytical Chemistry · August 1, 1998 This article provides an overview of the development and application of the surface-enhanced Raman scattering (SERS) techniques using metal-coated nanostructures on solid substrates. An introduction to theoretical principles of the SERS effect and the diff ... Full text Cite

Surface-enhanced Raman detection of nicotinamide in vitamin tablets

Journal Article Analytica Chimica Acta · July 17, 1998 Nicotinamide is analyzed quantitatively in vitamin tablets using surface-enhanced Raman scattering (SERS) on a silver-coated alumina substrate. Calibration curve of SERS signal intensity versus concentration of nicotinamide was established for quantitative ... Full text Cite

Near-Field Surface-Enhanced Raman Imaging of Dye-Labeled DNA with 100-nm Resolution.

Journal Article Analytical chemistry · July 1998 Raman chemical imaging on a scale of 100 nm is demonstrated for the first time. This is made possible by the combination of scanning near-field optical microscopy (SNOM or NSOM) and surface-enhanced Raman scattering (SERS), using brilliant cresyl blue (BCB ... Full text Cite

Surface-enhanced Raman gene probe for HIV detection.

Journal Article Analytical chemistry · April 1998 We report, for the first time, the use of surface-enhanced Raman (SERS)-active labels for primers used in polymerase chain reaction amplification of specific target DNA sequences. This method has the potential for combining the spectral selectivity and hig ... Full text Cite

Near-field surface-enhanced Raman spectroscopy of dye molecules adsorbed on silver island films

Journal Article Chemical Physics Letters · February 13, 1998 We report the combined use of scanning near-field optical microscopy and surface-enhanced Raman scattering (SERS) to obtain spectral, spatial and chemical information of molecular adsorbates with subwavelength lateral resolution. Near-field SERS spectra of ... Full text Cite

Laser-induced fluorescence for esophageal cancer and dysplasia diagnosis.

Journal Article Annals of the New York Academy of Sciences · February 1998 A method using laser-induced fluorescence (LIF) for in vivo cancer diagnosis of the esophagus is described. Autofluorescence of normal and malignant tissues was measured directly using a fiberoptic probe inserted through an endoscope. The measurements were ... Full text Cite

SERODS: A NEW MEDIUM FOR HIGH-DENSITY OPTICAL DATA STORAGE

Conference Optics InfoBase Conference Papers · January 1, 1998 Recently, extensive efforts have been devoted to investigating new generations of data storage devices based on nonmagnetic principles, such as optical data storage systems. Optical data storage systems can provide a unique combination of superior performa ... Cite

Diffraction tomographic imaging with photon density waves: An explicit solution

Journal Article Journal of the Optical Society of America A: Optics and Image Science, and Vision · January 1, 1998 An explicit formula is derived for reconstructing tissue inhomogeneities from scattered photon-density waves. When intensity-modulated light is used as a source, such waves obey a Helmholtz equation with a complex wave number. A process of analytical conti ... Full text Cite

Use of transmitted room-temperature phosphorescence to improve nalidixic acid determination

Journal Article Applied Spectroscopy · January 1, 1998 A method for nalidixic acid (NA) determination by transmitted room-temperature phosphorescence is proposed. The antibacterial agent NA in acidic solution shows native fluorescence; when NA is spotted on a filter paper precoated with β-cyclodextrin and lead ... Full text Cite

Development of a multiarray biosensor for DNA diagnostics

Journal Article Instrumentation Science and Technology · January 1, 1998 This work involves the development and evaluation of a multiarray biosensor for DNA diagnostics. The evaluation of various system components developed for the biosensor is discussed. The DNA probes labeled with visible and near infrared (NIR) dyes are eval ... Full text Cite

Proceedings of SPIE - The International Society for Optical Engineering: Introduction

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · 1998 Cite

Determination of Aluminium in Water through Formation of Fluorescent Dibenzoylmethane-Aluminium Complex and its Structure Elucidation

Journal Article Journal of the Indian Chemical Society · January 1, 1998 Dibenzoylmethane(DBM) was found to be a very good reagent for AlIII to produce a luminescent complex having the formula Al(DBM)3. The luminescence property of the complex was used to selectively quantify AlIII on filter paper substrate in the range of 5-55 ... Cite

A New Photoactivated Fluorescence Detection Method for Trichloroethylene

Journal Article Journal of the Indian Chemical Society · January 1, 1998 We describe here a novel method using photoactivated fluorescence for the detection of trichloroethylene (TCE). The approach involves photoactivation by uv irradiation of TCE in the presence of a photoactivator, diphenylamine, subsequent excitation of the ... Cite

Alstonine as a potential fluorescent marker for tiny tumours detection and imaging

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1997 3,4,5,6,16,17-Hexadehydro-16-(methoxycarbolyl)-19α-methyl-20α-o xyohimbanium (alstonine) is a fluorescent alcaloid which is known to stain tumor cells more efficiently than normals. The interactions between alstonine and biological macromolecules were firs ... Full text Cite

Erratum: Proceedings of Biomedical Sensing, Imaging, and Tracking Technologies II (11-13 February 1997)

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1997 Cite

Intracellular compartimentalization of Indo-1 and Mag-indo-1: Consequences on intracellular calcium and magnesium sensing and imaging for biomedical applications

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1997 Indo-1 and Mag-indo-1 are well known fluorescent probes which consist in two different cages of complexation covalently bound to the same fluorophor. The ionic trap of Indo-1 fit perfectly the size of the calcium ion and its sensitivity is well adapted to ... Full text Cite

Phosphorescence imaging system using an acousto-optic filter-based charge coupled device

Journal Article Analytica Chimica Acta · September 30, 1997 We are reporting for the first time the development of an imaging spectrometer for solid surface room temperature phosphorimetry based on an acousto-optic tunable filter. A He-Cd laser was employed as the excitation source, and a cooled two-dimensional cha ... Full text Cite

Evaluation of a Separation-Based Fiber-Optic Sensor in a Micellar Electrokinetic Capillary Chromatography Mode of Operation

Journal Article Analytical Chemistry · September 15, 1997 A fiber-optic sensor that integrates the separation-based selectivity of capillary electrophoresis with the sensitivity afforded by laser-induced fluorescence detection is evaluated. The evaluation involves measurements of neutral analytes that are separat ... Full text Cite

Phosphorescence imaging system using an acousto-optic tunable filter and a charge-coupled device

Journal Article Analytica Chimica Acta · July 21, 1997 We are reporting for the first time the development of an imaging spectrometer for solid-surface room temperature phosphorimetry based on an acousto-optic tunable filter. A HeCd laser was employed as the excitation source, and a cooled two-dimensional char ... Full text Cite

Development of a new capillary electrophoresis-based fibre optic sensor.

Journal Article Biomedical chromatography : BMC · July 1997 A new fluorescence-based fibre optic sensor is described which combines the sensitivity offered by laser-induced fluorescence with the selectivity offered by capillary electrophoresis (CE). A single optical fibre directly probes the terminus of a 5-8 cm se ... Full text Cite

Antibody-antigen binding kinetics. A model for multivalency antibodies for large antigen systems.

Journal Article Applied biochemistry and biotechnology · July 1997 This work presents a theoretical analysis of the influence of multivalency of antigen on external mass transfer-limited binding kinetics to divalent antibody for biosensor applications to polycyclic-aromatic systems. Both cases are considered wherein the a ... Full text Cite

Laser-induced differential fluorescence for cancer diagnosis without biopsy

Journal Article Applied Spectroscopy · January 1, 1997 An optical diagnostic procedure based on laser-induced fluorescence was developed for direct in vivo cancer diagnosis without requiring biopsy. The methodology was applied in a clinical study involving over 100 patients in order to differentiate normal tis ... Full text Cite

Introduction

Journal Article American Journal of Cardiology · January 1, 1997 Full text Cite

Enhanced Photoactivated Luminescence of Selected Polychlorinated Biphenyl Congeners and Aroclor Mixtures

Journal Article Microchemical Journal · January 1, 1997 Fluorescence studies of selected polychlorinated biphenyl (PCB) congeners and Aroclor mixtures were conducted using enhanced photoactivated luminescence. Diphenylamine (DPA) was used as a photoactivator. DPA reacts with PCBs to form a fluorescent photoprod ... Full text Cite

Vibrational spectra of the industrial dyes cresyl fast violet, phloxine B and saffron. Intensity studies by surface-enhanced Raman spectroscopy

Journal Article Analusis · December 1, 1996 The vibrational spectra, Fourier transform Raman, Fourier transform infrared, and surface-enhanced Raman scattering (SERS) spectra of the dyes cresyl fast violet, phloxine B, and saffron have been recorded and analyzed. The intensity of the intense SERS pe ... Cite

Laser-induced fluorescence detection of dysplasia in Barrett's esophagus

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1996 A study was conducted to determine whether laser-induced fluorescence could detect high grade dysplasia in Barrett's esophagus. Four-hundred-ten nm laser light was used to induce autofluorescence of Barrett's mucosa in 36 patients during routine endoscopy. ... Full text Cite

Detection of colon malignancy using differential normalized fluorescence

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1996 Laser-induced fluorescence was used for direct in-vivo diagnosis of colon malignancy without requiring biopsy. The methodology was applied in a clinical study in order to differentiate adenomatous polyps from hyperplastic polyps in the colon. The measureme ... Full text Cite

Multispectral imaging system using acousto-optic tunable filter (AOTF)

Conference Proceedings of SPIE - The International Society for Optical Engineering · November 13, 1996 This paper describes the development of a multispectral imaging (MSI) device using AN acousto-optic tunable filter (AOTF). A two-dimensional charge-coupled device (CCD) was used as a detector. The AOTF was used as a wavelength selector. Unlike a tunable gr ... Full text Cite

Demonstration of an integrated capillary electrophoresis-laser-induced fluorescence fiber-optic sensor.

Journal Article Talanta · November 1996 A unique integrated separation-based fiber-optic sensor for remote analysis, that incorporates capillary electrophoresis (CE) directly at the fiber sensing terminus is described for the first time. Based on laser-induced fluorescence detection, the sensor ... Full text Cite

Fiber optic sensor for laser-induced room-temperature phosphorescence detection of polycyclic aromatic compounds.

Journal Article Talanta · October 1996 The development of a fiber optic sensor for the analysis of polycyclic aromatic compounds based on laser-induced room-termperature phosphorimetry is reported for the first time. A pulsed nitrogen laser was used to excite phosphorescence emission from compo ... Full text Cite

Discrimination between tumour and normal cells by staining with 3,4,5,6,16,17-hexadehydro-16-(methoxycarbonyl)-19α-methyl-20α -oxayohimbanium: The uracil ring as a target for the specific interaction between RNA(s) and the fluorescent probe

Journal Article Anticancer Research · July 1, 1996 3,4,5,6,16,17-Hexadehydro-16-(methoxycarbonyl)-19α-methyl-20α -oxayohimbanium (Alstonine) is a fluorescent alcaloid which has been known to stain tumour cells more efficiently than normal ones. In this paper the spectral properties of Alstonine were first ... Cite

Endoscopic fluorescence detection of high-grade dysplasia in Barrett's esophagus.

Journal Article Gastroenterology · July 1996 Background & aimsEarly detection and treatment of esophageal cancer in Barrett's esophagus may improve patient survival if dysplasia is effectively detected at endoscopy. Typically, four-quadrant pinch biopsy specimens are taken at 2-cm intervals. ... Full text Cite

Development of a room-temperature phosphorescence fiber-optic sensor.

Journal Article Analytical chemistry · May 1996 The design of a new fiber-optic sensor based on solid-surface room-temperature phosphorimetry is presented for the analyses of polycyclic aromatic hydrocarbons in water samples. Analytical figures of merit are given for several compounds of environmental i ... Full text Cite

Antigen-antibody diffusion-limited binding kinetics for biosensors: A fractal analysis

Journal Article Sensors and Actuators, B: Chemical · January 1, 1996 A fractal analysis is presented for the binding of benzopyrene-tetraol (BPT) in solution to anti-BPT attached to diol silica beads in a fiber-optic antibody-based biosensor. The binding rate coefficient, k1, and the fractal dimension, Df, both increase as ... Full text Cite

Spectroscopy commissions of the International Union of Pure and Applied Chemistry

Journal Article Applied Spectroscopy · January 1, 1996 All chemists have heard of the International Union of Pure and Applied Chemistry, or IUPAC as it is usually called. Most chemists have regularly made use of the work of IUPAC, even if did not realize that they were doing so. Infrared spectroscopists have d ... Full text Cite

Remote spectral imaging system (RSIS) based on an acousto-optic tunable filter (AOTF)

Journal Article Instrumentation Science and Technology · January 1, 1996 This paper describes a new remote spectral imaging system (RSIS) based on an acousto-optic tunable filter (AOTF) capable of remote sensing using an imaging fiberoptic probe (IFP). A two-dimensional charge coupled device (CCD) was used as a detector. The AO ... Full text Cite

Fiber-optic remote multisensor system based on an Acousto-Optic Tunable Filter (AOTF)

Journal Article Applied Spectroscopy · January 1, 1996 This paper describes a new fiber-optic multisensor based on an acousto-optic tunable filter (AOTF) and capable of remote sensing using a multioptical fiber array (MOFA). A two-dimensional charge-coupled device (CCD) was used as a detector, and the AOTF was ... Full text Cite

Development of a genosensor for Mycobacterium tuberculosis

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1996 This work involves the development of a fiberoptic genosensor for the detection of Mycobacterium tuberculosis. A rapid and sensitive non-radioactive method for the detection of Mycobacterium is described. This method involves polymerase chain reaction (PCR ... Full text Cite

Determination of Enhancement Factors for Surface-Enhanced FT-Raman Spectroscopy on Gold and Silver Surfaces

Journal Article Journal of Raman Spectroscopy · January 1, 1996 The combination of surface-enhanced Raman spectroscopy (SERS) with near-infrared (NIR) excitation should be of considerable benefit for trace detection of biological and environmental samples. Enhancement factors for SERS of p-aminobenzoic acid (PABA) on s ... Full text Cite

Surface-enhanced Raman detection of nerve agent simulant (DMMP and DIMP) vapor on electrochemically prepared silver oxide substrates

Journal Article Journal of Raman Spectroscopy · January 1, 1996 Surface-enhanced Raman scattering (SERS) spectra of the chemical nerve agent simulants dimethyl methylphosphonate (DMMP) and diisopropyl methylphosphonate (DIMP) were obtained in the vapor phase. The spectra obtained demonstrate the possibility of detectin ... Full text Cite

Laser-induced solid-surface room-temperature phosphorimetry of polycyclic aromatic hydrocarbons

Journal Article Applied Spectroscopy · January 1, 1996 Laser-induced solid-surface room-temperature phosphorimetry (SSRTP) has been employed for the detection of polycyclic aromatic hydrocarbons. A nitrogen-pumped laser and a dye laser were used as excitation sources. The effects of sample volume, laser irradi ... Full text Cite

Laser-excited synchronous fluorescence system for the analysis of polycyclic aromatic compounds

Journal Article Polycyclic Aromatic Compounds · January 1, 1996 We describe a laser-based synchronous luminescence (LSL) monitor developed for the detection of polycyclic aromatic compounds (PAC). With LSL the amount of sample pre-treatment can often be reduced and both the selectivity and sensitivity of the analysis c ... Full text Cite

Antibody-based submicron biosensor for Benzo[a]pyrene DNA adduct

Journal Article Polycyclic Aromatic Compounds · January 1, 1996 Fiberoptic-based sensors have been developed for monitoring human exposure and health effects associated with polycyclic aromatic compounds such as benzo[a]pyrene. These sensors take advantage of the physical properties of optical fibers including their re ... Full text Cite

Analysis of polycyclic aromatic compounds in soil samples using laser-induced phosphorimetry

Journal Article Polycyclic Aromatic Compounds · January 1, 1996 The analysis and spectral characteristics of several polycyclic aromatic hydrocarbons (PAHs) have been investigated by Laser-Induced Solid-Surface Room Temperature Phosphorimetry (LI-SSRTP). These PAHs included phenanthrene, chrysene, 1,2 benzofluorene, 2, ... Full text Cite

Laser-excited sychronous flourescence spectrometer for benzo(a)pyrene

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1995 A laser-based synchronous scanning fluorimeter has been developed for the detection of polycyclic aromatic hydrocarbons (PAHs). Although extensive sample cleanup and separation are usually required, fluorescence has long been used for the determination of ... Cite

Field screening of polycyclic hydrocarbons contamination in soil using a portable synchronous scanning spectrofluorometer

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1995 Polycyclic aromatic hydrocarbons (PAH) contamination is a considerable problem at various hazardous waste sites. Sources of PAH contamination include incomplete combustion processes, wood preservatives, and the fuel industry. The development of rapid, cost ... Cite

Surface-enhanced Raman fiber optic sensors for remote monitoring

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1995 A new sensor design for remote surface-enhanced Raman scattering (SERS) measurements has been developed for environmental applications. The design features the modification of an optical fiber using layers of alumina microparticles and silver coatings for ... Cite

Fractal analysis for the development of chemical sensors for environmental monitoring

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1995 A fractal analysis is presented for the binding of pyrene in solution to beta-cyclodextrin attached to a fiberoptic chemical sensor. The specific (k 1) and the non-specific binding rate (k ns) coefficients and the fractal dimension, D f (specific binding c ... Cite

Development of gene-probe-based biosensors for environmental analysis

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1995 This work involves the design and development of species specific gene (DNA) probes designed to detect Pseudomonas species. A non radioactive detection system for DNA was designed to detect the presence of these microbes in environmental samples for biomon ... Cite

A beta-cyclodextrin based fiber-optic chemical sensor: a fractal analysis.

Journal Article Talanta · October 1995 A fractal analysis is presented for the binding of pyrene in solution to beta-cyclodextrin attached to a fiber-optic chemical sensor. The specific (k(l)) and non-specific binding rate coefficients and the fractal dimension (D(f)) (specific binding case onl ... Full text Cite

Identification of Polycyclic Aromatic Molecules in the UV Spectrum of Comet P/Halley

Journal Article Polycyclic Aromatic Compounds · July 1, 1995 Emission spectra of comet P/Hallev in the 275–710 nm wavelength range were obtained by using a spectrometer carried on board the Vega 2 spacecraft which encountered the famous comet on March 9, 1986. The spectrum, after subtraction of the intense solar con ... Full text Cite

Spectroscopic diagnosis of esophageal cancer: new classification model, improved measurement system.

Journal Article Gastrointestinal endoscopy · June 1995 Laser-induced fluorescence spectroscopy was used to measure fluorescence emission of normal and malignant tissue during endoscopy in patients with esophageal cancer and volunteers with normal esophagus. The spectroscopy system consisted of a nitrogen-pumpe ... Full text Cite

IN vivo cancer diagnosis of the esophagus using laser-induced fluorescence

Conference Proceedings of SPIE - The International Society for Optical Engineering · April 12, 1995 Laserinduced fluorescence (LIF) was used for direct in-viva cancer diagnosis of the esophagus without requiring biopsy. The methodology was applied to differentiate normal and malignant tumors of the esophagus. Endogenous fluorescence of normal and maligna ... Full text Cite

Analysis of polynuclear aromatic compounds using laser-excited synchronous fluorescence

Journal Article Analytica Chimica Acta · March 10, 1995 Monitoring the presence and distribution of polynuclear aromatic compounds (PACs) in the environment is an important task due to the presence of these species in many types of environmental samples. Molecular fluorescence is often used for this task, usual ... Full text Cite

Nomenclature, Symbols, Units and their Usage in Spectrochemical Analysis—Xi detection of radiation

Journal Article Pure and Applied Chemistry · January 1, 1995 This report is 11th in the series on Spectrochemical Methods of Analysis issued by IUPAC Commission V.4. It is concerned with Radiation Detection as used in analytical atomic and molecular emission, absorption, and fluorescence spectroscopy in the X-ray an ... Full text Cite

Selective Surface-Enhanced Raman Spectroscopy Using a Polymer-Coated Substrate

Journal Article Analytical Chemistry · January 1, 1995 The polymer, poly(vinylpyrrolidone), is investigated as a potential coating for alumina-based surface-enhanced Raman scattering (SERS) substrates. Description for dipcoating alumina substrates with a 5% (w/v) methanolic solution of the polymer is included. ... Full text Cite

Capillary Electrophoresis—Laser Fluorometry Instrumentation for the Facile Optimization of DNA Separations Using in Situ Size-Selective Gradients and Adjustable Detection Zone

Journal Article Analytical Chemistry · January 1, 1995 The construction and evaluation of a capillary electrophoresis instrument for the facile optimization of DNA separations is described herein. This instrumentation allows for convenient translation of the detection zone with little loss in detectability. Ch ... Full text Cite

Fast scanning synchronous luminescence spectrometer based on acousto-optic tunable filters

Journal Article Appl. Spectrosc. (USA) · 1995 A new luminescence spectrometer based on quartz-collinear acousto-optic tunable filters (AOTFs) and capable of synchronous scanning is described. An acousto-optic tunable filter is an electronically tunable optical bandpass filter. Unlike a tunable grating ... Cite

SERS chemical sensors and biosensors: new tools for environmental and biological analysis

Journal Article Sensors and Actuators, B: Chemical · 1995 This paper describes recent advances in chemical sensors and biosensors based on surface-enhanced Raman scattering (SERS) detection. The SERS-active probes involve silver-coated microstructured substrates designed to amplify the Raman signals of adsorbed m ... Full text Link to item Cite

SERS chemical sensors and biosensors: new tools for environmental and biological analysis

Journal Article Sensors and Actuators: B. Chemical · 1995 This paper describes recent advances in chemical sensors and biosensors based on surface-enhanced Raman scattering (SERS) detection. The SERS-active probes involve silver-coated microstructured substrates designed to amplify the Raman signals of adsorbed m ... Cite

Evaluation of data treatment techniques for improved analysis of fingerprint images

Journal Article Journal of Forensic Sciences · January 1, 1995 We evaluated four different methods for data treatment of fingerprint images. The first two methods (Lee's method, and Iterative Automated Noise Filtering method) are used to remove signal-independent additive noise from digital images of fingerprints. The ... Full text Cite

In vivo cancer diagnosis of the esophagus using differential normalized fluorescence (DNF) indices.

Journal Article Lasers in surgery and medicine · January 1995 Background and objectiveWe report the use of new diagnostic parameters based on the differential normalized fluorescence (DNF) signals for malignant tumor diagnosis.Study design/materials and methodsOver 200 measurements of endogenous flu ... Full text Cite

Mixture analysis using a compact SERS spectrometer

Journal Article Analusis · January 1, 1995 This paper describes the use of a simple, compact, portable spectrometer designed for field measurement using surface-enhanced Raman scattering (SERS) analysis. The instrument yields excellent sensitivity for a wide variety of chemical species, with detect ... Full text Cite

Surface-enhanced Raman optical data storage: A new optical memory with three-dimensional data storage

Journal Article Review of Scientific Instruments · December 1, 1994 A new optical data storage technology based on the surface-enhanced Raman scattering (SERS) effect has been developed for high-density optical memory and three-dimensional data storage. With the surface-enhanced Raman optical data storage (SERODS) technolo ... Full text Cite

Surface-enhanced Raman gene probes.

Journal Article Analytical chemistry · October 1994 We report for the first time a new type of DNA gene probe based on surface-enhanced Raman scattering (SERS) label detection. The surface-enhanced Raman gene (SERG) probes do not require the use of radioactive labels and have great potential to provide both ... Full text Cite

Detection of cadmium ion using the fluorescence probe Indo-1

Journal Article Analytica Chimica Acta · September 9, 1994 Fluorescence probes for biosensors used for the determination of intracellular concentration of metal ions have been synthesized in order to bind selectivity with a specific cation. Since certain ions exhibit similar chemical properties, binding selectivit ... Full text Cite

A portable surface-enhanced raman spectrometer

Journal Article Instrumentation Science & Technology · August 1, 1994 We describe the development of a simple and compact spectrometer for use in Raman and Surface-enhanced Raman Scattering (SERS) analysis. The instrument using an avalanche photodiode is designed to use under field conditions with simple optical samples. Exa ... Full text Cite

Fluorescence spectroscopy for diagnosis of esophageal cancer

Conference Proceedings of SPIE - The International Society for Optical Engineering · July 19, 1994 Laser-induced fluorescence spectroscopy was employed to measure fluorescence emission of normal and malignant tissue during endoscopy in patients with esophageal adenocarcinoma. A nitrogen/dye laser tuned at 410 nm was used for excitation source. The fluor ... Full text Cite

Synchronous luminescence: a new detection technique for multiple fluorescent probes used for DNA sequencing.

Journal Article BioTechniques · June 1994 A method fo nonradioactive DNA sequencing, which has been marketed commercially, uses four different fluorescent tags to label DNA fragments and fixed-wavelength excitation/fluorescence detection of the labels. This study presents an alternative method of ... Cite

Photoactivated Luminescence Method for Rapid Screening of Polychlorinated Biphenyls

Journal Article Analytical Chemistry · April 15, 1994 This paper describes a new method based on enhanced photoactivated luminescence (EPL) for rapid detection of polychlorinated biphenyls (PCBs). The EPL method described here is a unique procedure combining several processes including (i) photoactivation by ... Full text Cite

Gel-Based Indo-1 Probe for Monitoring Calcium(II) Ions

Journal Article Analytical Chemistry · March 15, 1994 This work describes the development of a new gel-based Indo-1 (GBI) fluorescent probe for use in detection of Ca2+. The carbodiimide-binding procedure, which allows the formation of an amide bond between the aromatic carboxylic group of Indo-1 and an amino ... Full text Cite

SERODS: principle of a new optical data storage system

Journal Article Proc. SPIE - Int. Soc. Opt. Eng. (USA) · 1994 This paper describes the basic principle of a new approach for large-memory optical disk storage based on the surface-enhanced Raman optical data storage (SERODS) technology. With the SERODS technology, the molecular interactions between the optical layer ... Cite

Biosensors and antibody probes for environmental and biomedical applications

Journal Article Proc. SPIE - Int. Soc. Opt. Eng. (USA) · 1994 This paper presents an overview of the applications of antibody-based fiber optic sensors. Special focus is devoted to antibody-based fiber optic fluoroimmunosensors developed in our laboratories to detect important pollutants and biochemicals. The fiber o ... Cite

Winefordner and molecular spectroscopy-The man and his legacy

Journal Article Spectrochimica Acta - Part B Atomic Spectroscopy · January 1, 1994 Full text Cite

Oriented Si and Ge nanocrystals formed in Al2O3 by ion implantation and annealing

Journal Article Materials Research Society Symposium Proceedings · January 1, 1994 Ion implantation followed by thermal annealing in a reducing atmosphere has been used to create a high density of oriented Si and Ge nanocrystals in (0001) Al2O3. Both types of nanocrystals are three-dimensionally aligned with respect to the Al2O3 matrix, ... Cite

Vibrational spectral analysis of Eosin Y and Erythrosin B—intensity studies for quantitative detection of the dyes

Journal Article Journal of Raman Spectroscopy · January 1, 1994 The complete vibrational spectra of Eosin Y and Erythrosin B were recorded using Fourier transform Raman and Fourier transform infrared spectroscopy. The surface‐enhanced Raman scattering (SERS) spectra of these two dyes were also studied using an intensif ... Full text Cite

Development of a battery-operated portable synchronous luminescence spectrofluorometer

Journal Article Review of Scientific Instruments · December 1, 1993 A battery-operated portable synchronous luminescence spectrofluorometer was developed for on-site analysis of groundwater or hazardous waste sites. The instrument is capable of either emission, excitation, or synchronous fluorescence measurements. The inst ... Full text Cite

Immunosensors: Principles and Applications

Journal Article ImmunoMethods · October 1, 1993 This paper presents an overview of the principles and applications of immunofluorescence spectroscopy in the development of antibody-based fiber-optic sensors. Special focus is devoted to antibody-based fiber-optic fluoroimmunosensors developed in our labo ... Full text Cite

Measurement of DNA adducts using surface-enhanced Raman spectroscopy.

Journal Article Journal of toxicology and environmental health · October 1993 Hazardous pollutants emitted from energy-related technologies, chemical industries, or waste materials are of increasing public concern because of their potential adverse health effects. Many pollutants have chemical groups of toxicological importance that ... Full text Cite

Polycyclic aromatic hydrocarbons in the atmospheres of Titan and Jupiter.

Journal Article The Astrophysical journal · September 1993 Polycyclic aromatic hydrocarbons (PAHs) are important components of the interstellar medium and carbonaceous chondrites, but have never been identified in the reducing atmospheres of the outer solar system. Incompletely characterized complex organic solids ... Full text Cite

Surface-enhanced Raman scattering analysis of etheno adducts of adenine

Journal Article Vibrational Spectroscopy · March 5, 1993 We report surface-enhanced Raman spectroscopy (SERS) detection and analysis of several etheno adducts formed from the metabolism of vinyl chloride: 1,N6-ethenoadenine, 1,N6-ethenoadenosine, and 1,N6-etheno-2′-deoxyadenosine. Spectra of the etheno adducts a ... Full text Cite

Vibrational spectra of fluvalinate

Journal Article Journal of Raman Spectroscopy · January 1, 1993 The normal Raman spectrum (NRS), Fourier transform infrared spectrum (FTIRS) and surface‐enhanced Raman spectrum (SERS) of the important insecticide and acaricide fluvalinate (C26H22ClF3N2O3) were recorded for the first time. The spectral data are discusse ... Full text Cite

Surface-Enhanced Raman Analysis of Some Polycyclic Aromatic Dyes Used in the Cosmetics and Food Industries

Journal Article Polycyclic Aromatic Compounds · January 1, 1993 The Surface-Enhanced Raman spectra of three important polycyclic aromatic dyes used in the food and cosmetic industries. Erythrosine. Eosine, and Phloxine, are recorded for the first time. The ability of the Surface-Enhanced Raman scattering techniques to ... Full text Cite

Surface-enhanced raman vapor dosimeter

Journal Article Applied Spectroscopy · 1993 Cite

Laser-excited synchronous luminescence spectroscopy

Journal Article Applied Spectroscopy · 1993 Cite

Permeation measurements of chemical agent simulants through protective clothing materials

Journal Article Journal of Hazardous Materials · January 1, 1993 Effective procedures associated with storage and disposal of chemical warfare (CW) agents are important for the protection of civilian populations from inadvertent release of these agents. Emergency groups as well as citizens in surrounding communities nee ... Full text Cite

Polycyclic aromatic hydrocarbons in the atmospheres of Titan and Jupiter

Journal Article Astrophysical Journal · 1993 Polycyclic aromatic hydrocarbons (PAHs) are important components of the interstellar medium and carbonaceous chondrites, but have never been identified in the reducing atmospheres of the outer solar system. Incompletely characterized complex organic solids ... Cite

Intensified Charge Coupled Device-Based Fiber-Optic Monitor for Rapid Remote Surface-Enhanced Raman Scattering Sensing

Journal Article Applied Spectroscopy · November 1, 1992 This paper describes the development of an intensified charge coupled device (ICCD)-based fiber-optic monitor for remote Raman and surface-enhanced Raman (SERS) sensing. Both Raman and SERS data were obtained with the use of a fiber-optic probe design inco ... Full text Cite

Surface-Enhanced Raman Analysis of p-Nitroaniline on Vacuum Evaporation and Chemically Deposited Silver-Coated Alumina Substrates

Journal Article Applied Spectroscopy · September 1, 1992 In this study we investigated the surface-enhanced Raman scattering (SERS) properties of p-nitroaniline, which is a potential chemical for use in optical disk coating(s) and also an important compound used as an intermediate or precursor in the manufacture ... Full text Cite

Improved Methods for Screening of Polychlorinated Biphenyls (PCBs) Using Room-Temperature Phosphorescence

Journal Article Applied Spectroscopy · August 1, 1992 Several methods for the improvement of room-temperature phosphorescence (RTP) analysis of polychlorinated biphenyls (PCBs) have been investigated. Two approaches that were investigated in order to detect improvements over existing methods were the second-d ... Full text Cite

Investigation of noncalcium interactions of fura-2 by classical and synchronous fluorescence spectroscopy.

Journal Article Analytical biochemistry · August 1992 Several authors have reported unexpected intracellular spectra of both indo-1 and fura-2. One of the major methodological problems in the evaluation of calcium concentration using fluorescent probes is that it is assumed that only two forms of the dyes are ... Full text Cite

DNA adduct formation by 12 chemicals with populations potentially suitable for molecular epidemiological studies.

Journal Article Mutation research · July 1992 DNA adduct formation, route of absorption, metabolism and chemistry of 12 hazardous chemicals are reviewed. Methods for adduct detection are also reviewed and approaches to sensitivity and specificity are identified. The selection of these 12 chemicals fro ... Full text Cite

Passive dosimeter for monitoring ammonia vapor

Journal Article Analytica Chimica Acta · June 15, 1992 A method is presented for ammonia detection in the vapor phase that exploits the reaction between fluorescamine and ammonia on a sodium lauryl sulfate (NaLS)-treated paper substrate. The fluorescence intensity of the aquamarine fluorescence product is used ... Full text Cite

Development of a Fluorescence Quenching Technique to Detect Permeation of Chemical Agent Simulants through Protective Clothing Materials

Journal Article Applied Spectroscopy · April 1, 1992 This study involves the development of a new and simple analytical procedure based on a spectrochemical modification (SM) technique, namely fluorescence quenching (FQ) to monitor chemical agent simulants. The FQ technique is applied to evaluate permeation ... Full text Cite

Screening Benzo(a)pyrene Metabolites in Urine Using Synchronous Room Temperature Phosphorescence

Journal Article Polycyclic Aromatic Compounds · January 1, 1992 We have developed a simple measurement procedure for screening of polycyclic aromatic hydrocarbons (PAC) and their metabolites in urine using room temperature phosphorescence (RTP). The signal intensities were proportional to the amounts of benzo(a)pyrene- ... Full text Cite

Currently available permeability and breakthrough data characterizing chemical warfare agents and their simulants in civilian protective clothing mater

Journal Article Journal of Hazardous Materials · January 1, 1992 The current analysis characterizes chemical protective clothing (CPC) that would be available to civilian emergency personnel responding to possible ne. © 1992. ... Full text Cite

Laser-based fiberoptic lmmljnosensors

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1992 This paper provides a brief overview of the development and application of fiber-optic antibody-based fluoroimmunosensors (FIS) for measuring environmental pollutants and related biomarkers of human exposure and health effects. The FIS combines the excelle ... Full text Cite

Surface-enhanced Raman scattering interaction of p-aminobenzoic acid on a silver-coated alumina substrate

Journal Article Spectrochim. Acta A, Mol. Spectrosc. (UK) · 1992 The adsorption behavior of p-aminobenzoic acid (PABA) molecules on a silver-coated alumina surface-enhanced Raman scattering (SERS) substrate was investigated. For spotted PABA and PABA in non-polar solvents, the PABA molecule is adsorbed flat on the surfa ... Full text Link to item Cite

A rapid field screening method for polychlorinated b1phenyls (pcbs)

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1992 The analysis of polychlorinated biphenyls (PCBs) generally requires selectivity and sensitivity. Even after cleanup, PCBs are usually at ultratrace levels in field samples, mixed in with other halocarbons, hydrocarbons, lipids, etc. The levels of PCBs typi ... Full text Cite

Chemical monitors based on surface-enhanced raman scatiering (sers)

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1992 This paper presents an overview of the development of chemical monitors using the Surface-Enhanced Raman Scattering (SERS) technique. The SERS effect is based on recent experimental observations, which have indicated enhancement of the Raman scattering eff ... Full text Cite

Portable surface-enhanced Raman toxic chemical analyzer development

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1992 GAMMA-METRICS and Oak Ridge National Laboratory have developed a prototype portable toxic chemical analyzer (TCA) for environmental screening using surface enhanced Raman scattering technology. The focus is on detection of anthropogenic chemicals such as p ... Cite

Optical monitor for microbial metabolism for hazardous waste application

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1992 A remote detection system for in-situ monitoring of the bioluminescence produced by a microbial `reporter' strain grown in rotary film bioreactors has been developed. Two systems were developed: one using a low-temperature photomultiplier, and one using an ... Cite

Surface-enhanced Raman scattering interaction of p-aminobenzoic acid on a silver-coated alumina substrate

Journal Article Spectrochimica Acta Part A: Molecular Spectroscopy · 1992 The adsorption behavior of p-aminobenzoic acid (PABA) molecules on a silver-coated alumina surface-enhanced Raman scattering (SERS) substrate was investigated. For spotted PABA and PABA in non-polar solvents, the PABA molecule is adsorbed flat on the surfa ... Cite

Comparative pharmacokinetics of the photosensitizer tin-etiopurpurin in dogs and rats

Journal Article Journal of Veterinary Pharmacology and Therapeutics · 1992 Photodynamic therapy is a promising new treatment for local eradication of cancer. Little work has been done to define the pharmacokinetics of photodynamic drugs or the variability in drug disposition that may occur between different species and pathophysi ... Cite

Normal Raman and surface‐enhanced Raman scattering (SERS) spectra of some fungicides and related chemical compounds

Journal Article Journal of Raman Spectroscopy · January 1, 1992 Surface‐enhanced Raman scattering (SERS) spectra of Thiram (C6H12N2S4) and Ferbam (C9H18N3S6Fe); and normal Raman scattering (NRS) spectra of Thiram and Metam‐Sodium (C2H4NNaS2) were investigated. The SERS spectra of the two compounds and NRS spectrum of M ... Full text Cite

A multi-optical-fiber array with charge-coupled device image detection for parallel processing of light signals and spectra

Journal Article Review of Scientific Instruments · December 1, 1991 We describe a new two-dimensional multioptical-fiber array (MOFA) with 144 elements, its use with charge-coupled device (CCD) image detection to perform parallel processing for light intensity measurements, and its use with a concave aspherical grating and ... Full text Cite

Construction and evaluation of a regenerable fluoroimmunochemical-based fibre optic biosensor

Journal Article The Analyst · December 1, 1991 A microscale fibre optic biosensor that is capable of in situ regeneration is described and characterized. By combining recently developed fibre optic sensing technology with a capillary column reagent delivery system, it is possible to perform a variety o ... Full text Cite

A fiber-optic cyclodextrin-based sensor.

Journal Article Talanta · May 1991 This paper describes the development of a fiber-optic cyclodextrin-based (FCD) sensor. The device uses laser excitation and fluorescence detection with beta-cyclodextrin as the reagent phase, immobilized at the tip of an optical fiber. The sensitivity of t ... Full text Cite

Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples.

Journal Article Clinical chemistry · April 1991 This paper presents the application of a fiber-optic antibody-based fluoroimmunosensor (FIS) for measuring DNA adducts of benzo[alpha]pyrene in human placenta samples. The FIS combines the excellent specificity of the antigen-antibody reaction, the high se ... Cite

Fiber optic sensor probe for in-situ surface-enhanced Raman monitoring

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1991 In this paper we report on the development of a fiber optic sensor using a new detection method based on the surface-enhanced Raman scattering (SERS) technique. The SERS effect is based on recent experimental observations, which have indicated enhancement ... Cite

Advanced approaches in luminescence and Raman spectroscopy

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1991 An overview is given of advanced analytical techniques and instrumentation, such as surface-enhanced Raman scattering (SERS) and antibody-based fiber- optic sensors, used to detect trace levels of chemical pollutants and related biomarkers in complex envir ... Cite

Surface-enhanced Raman analysis of vitamin B complex: quantitative detection of p-aminobenzoic acid

Journal Article J. Raman Spectrosc. (UK) · 1991 Surface-enhanced Raman scattering (SERS) spectra of vitamin B complex extracted in ethanol were analyzed using a silver-coated alumina substrate. The SERS effect appears to enhance selectively the spectrum of p-aminobenzoic acid (PABA) from among the compl ... Cite

Surface-Enhanced Raman spectroscopy for remote sensing

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1990 Conventional Raman spectroscopy is often limited by its low sensitivity due to the inherently weak Raman cross section of organic chemicals. A relatively new detection technique, Surface-Enhanced Raman Scattering (SERS) spectroscopy is based on recent expe ... Cite

Advances in surface-enhanced Raman spectroscopy for hazardous waste monitoring

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · December 1, 1990 Surface-enhanced Raman spectroscopy is being evaluated for use as an advanced method for detecting organic contaminants in groundwater during field-screening of environmental samples. The SERS technique offers attractive and unique capabilities for detecti ... Cite

Fiber-Optic Remote Sensor for in Situ Surface-Enhanced Raman Scattering Analysis

Journal Article Analytical Chemistry · November 1, 1990 An in situ SERS fiber-optic system for remote sensing was developed for exciting and collecting surface-enhanced Raman scattering (SERS) signals generated from a sensing probe having silver-coated microparticles deposited on a glass plate support. The geom ... Full text Cite

Room temperature phosphorescence detection for paper electrophoresis (rtp-pe)

Journal Article Analytical Letters · May 1, 1990 This paper reports for the first time room-temperature phosphorescence applied to paper electrophoresis (RTP-PE). Examples are illustrated for benzo[a]pyrene-r-7, t-8, 9, 10-tetrahydrotetro! (BPT) and pyrene which have been detected by RTP after separation ... Full text Cite

Laser Techniques in Luminescence Spectroscopy

Conference ASTM Special Technical Publication · January 1, 1990 Full text Cite

Laser Induced Luminescence Spectroscopy on Solid Substrates: An Overview

Conference ASTM Special Technical Publication · January 1, 1990 This paper provides an overview of the photophysical processes and analytical applications of high-resolution luminescence spectroscopy using solid samples. Experimental results are discussed within the framework of a theoretical model involving isolated g ... Full text Cite

Fibre-optic-based fluoroimmunosensors

Journal Article Philos. Trans. R. Soc. A, Phys. Sci. Eng. (UK) · 1990 Remote sensing of chemicals can be performed using fibre optic chemical sensors that use immunochemical reagent phases. Exploiting the specificity of antibody-antigen interactions and the sensitivity of laser-excited fluorimetry, highly selective measureme ... Cite

Phase-resolved fiber-optics fluoroimmunosensor

Journal Article Applied Spectroscopy · January 1, 1990 This paper describes the development and application of a phase-resolved fiber-optics fluoroimmunosensor (PR-FIS), which combines the biochemical specificity of antigen-antibody reaction, the high sensitivity of laser excitation, the versatility of fiber-o ... Full text Cite

Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE)

Journal Article Applied Spectroscopy · January 1, 1990 This paper describes a new instrument system combining capillary-zone electrophoresis (CZE) separation and charge-coupled device (CCD) fluorescence detection. The performance of this CCD-CZE system is evaluated and compared with the performance of a photom ... Full text Cite

Canine treatment with SnET2 for photodynamic therapy

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · January 1, 1990 Photodynamic therapy is a treatment technique that utilizes the photoactived species of a drug to destroy tumor tissue. To be successful, the drug must localize in tumor tissue preferentially over normal tissue and must be activated by light of a specific ... Full text Cite

Surface-enhanced Raman scattering fiber-optic sensor

Journal Article Applied Spectroscopy · January 1, 1990 A fiber-optic system was developed for exciting and collecting surface-enhanced Raman scattering (SERS) signals generated from a sensing plate tip having silver-coated microparticles deposited on a glass support. Various fiber parameters, such as fiber typ ... Full text Cite

A fiber optic antibody-based biosensor with time-resolved detection

Journal Article Proc. SPIE - Int. Soc. Opt. Eng. (USA) · 1990 A phase-resolved fiber optic antibody-based biosensor is described, which combines the biochemical specificity of antigen-antibody reaction, the high sensitivity of laser excitation, the versatility of fiber optic sensors, and the selectivity of phase-reso ... Cite

Paper electrophoresis with surface-enhanced fluorimetric detection

Journal Article Analytica Chimica Acta · January 1, 1990 Amorphous fumed silica was used as a fluorescence sensitizer coated on a filter-paper substrate for use in paper electrophoresis. The usefulness of the separation method was illustrated for a mixture of two polynuclear aromatic compounds of biological inte ... Full text Cite

Evaluation of antibody immobilization technqiues for fiber optic-based fluoroimmunosensing

Journal Article Analytica Chimica Acta · January 1, 1990 Fiber optic chemical sensors have been developed that employ immunochemicals to perform fluoroimmunoassays. A regenerable fiber optic sensor was developed with a capillary delivery system to perform repetitive assays using antibodies immobilized to beads, ... Full text Cite

Fluorescence monitoring of a benzo[a]pyrene metabolite using a regenerable immunochemical-based fiber-optic sensor

Journal Article Analytica Chimica Acta · January 1, 1990 Microscale regenerable biosensors are described and utilized to measure the natural fluorophor benzo[a]pyrene tetraol (BPT). The sensors combine laser-excited/fiber-optic remote sensing principles with a unique capillary tube delivery system to make repeti ... Full text Cite

Fumed Silica Substrates for Enhanced Fluorescence Spot Test Analysis of Benzo[a]pyrene-DNA Adduct Products

Journal Article Analytical Chemistry · January 1, 1989 Amorphous fumed silica has been found to enhance both the Intensity and resolution of the emission, excitation, and synchronous fluorescence spectra of benzo[a]pyrene-r-7,t-8,9,10-tetrahydrotetrol (BPT) on a filter paper substrate. Experimental parameters ... Full text Cite

Silver-coated alumina as a new medium for surfaced-enhanced Raman scattering analysis

Journal Article Applied Spectroscopy · January 1, 1989 A new and simple substrate for inducing surface-enhanced Raman scattering (SERS) was investigated. This new SERS substrate consists of a solid support, such as a microscope slide, coated with alumina and then covered with silver. The alumina used in this w ... Full text Cite

Novel surface-enhanced fluorescence detection of polynuclear aromatic hydrocarbons separated by paper chromatography

Journal Article Analytical Letters · January 1, 1989 Amorphous fumed silica was used to enhance the fluorescence signal of a mixture of polynuclear aromatic (PNA) compounds separated by paper chromatography. Benzo[a]pyrene-r-7, t-8, 9, 10-tetrahydrotetrol (BPT) and benzo[a]pyrene (BaP) were well resolved by ... Full text Cite

Silver-Coated Fumed Silica as a Substrate Material for Surface-Enhanced Raman Scattering

Journal Article Analytical Chemistry · January 1, 1989 A new and simple type of substrate for surface-enhanced raman scattering (SERS) was investigated. This new substrate is based upon sliver-coated fumed silica on surfaces such as glass plates or microscopic slides. Fumed silica, which has submicrometer size ... Full text Cite

Surface-active substrates for Raman and luminescence analysis.

Journal Article Talanta · January 1989 This paper describes the development of active materials for optically enhanced Raman and fluorescence spectroscopy. The substrates for surface-enhanced Raman scattering investigated in this study involved silver-coated microspheres on glass plates. The ef ... Full text Cite

Titanium Dioxide Based Substrate for Optical Monitors in Surface-Enhanced Raman Scattering Analysis

Journal Article Analytical Chemistry · January 1, 1989 An optical monitor was investigated for in situ surface-enhanced Raman scattering (SERS) analysis. This optical monitor consists of a solid surface such as a glass plate coated with TiO2 and covered with a sllver layer. The TiO2 provides the necessary surf ... Full text Cite

Studies of cyclodextrin-enhanced room-temperature phosphorescence

Journal Article Analytica Chimica Acta · January 1, 1989 The enhancement of room-temperature phosphorescence by α-, β- and γ-cyclodextrins in the presence of heavy atoms is described for p-aminobenzoic acid, anthracene and six of its derivatives, dibenzofuran and some other compounds. The sensitivity can be impr ... Full text Cite

Evaluation of protective clothing materials challenged by petroleum and synfuel fluids

Journal Article ASTM Special Technical Publication · December 1, 1988 The permeation characteristics of eleven petroleum, coal, and shale oil hydrocarbon liquids through eight different types of glove were measured over 24 h. Two analytical techniques, photoionization and room-temperature phosphorescence of polynuclear aroma ... Cite

Development of antibody-based fiber-optic sensors

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · June 21, 1988 The speed and specificity characteristic of immunochemical complex formation has encouraged the development of numerous antibody-based analytical techniques. The scope and versatility of these established methods can be enhanced by combining the principles ... Full text Cite

Immunofluorescence Detection for Fiber Optics Chemical and Biological Sensors

Journal Article Proceedings of SPIE - The International Society for Optical Engineering · April 27, 1988 This paper presents the development and application of immunofluorescence spectroscopy in fiberoptics-based chemical and biological sensors. Focus is on a fluoroimmuno-sensor (FIS), which combines the excellent specificity of the antigen-antibody reaction ... Full text Cite

Fluorescence Detection of Phototoxic Psoralens in Vegetable Products

Journal Article Journal of Agricultural and Food Chemistry · March 1, 1988 This work describes the fluorescence detection of phototoxic psoralens in various vegetable products including celery, lime, carrot, parsley, and spinach. The spectral properties of psoralen, 5-methoxypsoralen, and 8-methoxypsoralen and raw extracts of cel ... Full text Cite

Selective Enhancement of Room Temperature Phosphorescence Using Cyclodextrin-Treated Cellulose Substrate

Journal Article Analytical Chemistry · March 1, 1988 The selective enhancement of room temperature phosphorescence (RTP) for a wide variety of polynuclear aromatic hydrocarbons (PNAs) on cyclodextrln-treated filter paper was Investigated. Filter paper substrates were treated by simple Impregnation In saturat ... Full text Cite

Fiber-Optic time-resolved fluorimetry for immunoassays.

Journal Article Talanta · February 1988 Rare-earth metal chelates used as fluorescent labels for immunoassays possess extremely long fluorescence lifetimes and permit the elective use of time-resolved detection. This is very important in fiber-optic fluorimetry, a technique that ordinarily exhib ... Full text Cite

ROOM-TEMPERATURE PHOSPHORIMETRY TO STUDY PETROLEUM PRODUCT PERMEATION THROUGH PROTECTIVE CLOTHING MATERIALS.

Journal Article Applied Spectroscopy · January 1, 1988 A simple analytical tool based on room-temperature phosphorimetry (RTP) is developed and used for evaluating the effectiveness of protective clothing materials against permeation of organic substances containing compounds such as the polycyclic aromatic co ... Full text Cite

Surface-enhanced Raman spectrometry of chlorinated pesticides

Journal Article Analytica Chimica Acta · January 1, 1988 The utility of surface-enhanced Raman scattering (SERS) spectrometry for trace determination of various chlorinated pesticides including chlordan, carbophenothion, bromophos, methyl chloropyrifos, dichloran, linuron, and 1-hydroxychlordene is described. Si ... Full text Cite

Recent advances in surface-enhanced Raman spectrometry for chemical analysis

Journal Article Spectrochimica Acta Part B: Atomic Spectroscopy · January 1, 1988 Surface-Enhanced Raman Scattering (SERS) is a relatively new approach in chemical analysis whereby the Raman scattering efficiency can be enhanced by factors of 103 to 107 when a compound is adsorbed on or near roughened metal surfaces, submicron structure ... Full text Cite

Surface-enhanced Raman spectrometry of chlorinated pesticides

Journal Article Analytica Chimica Acta · January 1, 1988 The utility of surface-enhanced Raman scattering (SERS) spectrometry for trace determinations of various chlorinated pesticides including chlordan, carbophenothion, bromophos, methyl chloropyrifos, dichloran, linuron, and l-hydroxychlordene is described. S ... Full text Cite

SITE-SELECTION PHOSPHORIMETRY VIA SINGLET-STATE EXCITATION.

Journal Article Applied Spectroscopy · January 1, 1988 This paper describes the technique of excitation energy site-selection (SS) phosphorimetry for chemical analysis of polycyclic aromatic compounds in Shpolskii matrices at 4. 2 K. The principle of the SS phosphorescence technique, which uses excitation via ... Full text Cite

Surface-enhanced Raman spectrometry of organophosphorus chemical agents.

Journal Article Analytical chemistry · September 1987 Full text Cite

Evaluation of an improved fiberoptics luminescence skin monitor with background correction.

Journal Article American Industrial Hygiene Association journal · June 1987 In this work, an improved version of a fiberoptics luminescence monitor, the prototype luminoscope II, is evaluated for in situ quantitative measurements. The instrument was developed to detect traces of luminescing organic contaminants on skin. An electro ... Full text Cite

Development of luminescence procedures to evaluate permeation of multi-ring polyaromatic compounds through protective materials.

Journal Article American Industrial Hygiene Association journal · April 1987 This study describes two practical and simple luminescence techniques developed to evaluate the efficacy of protective clothing materials against permeation of multi-ring polyaromatic compounds contained in heavy oil and petroleum products. The procedures ... Full text Cite

ANTIBODY-BASED FIBEROPTICS BIOSENSOR FOR THE CARCINOGEN BENZO(A)PYRENE.

Journal Article Applied Spectroscopy · January 1, 1987 A new antibody-based fiberoptics biosensor was used to detect the important carcinogen benzo(a)pyrene (BaP). The fiberoptics sensor utilizes anti-BaP antibodies covalently bound to its tip. A helium cadmium laser was used as the excitation source to induce ... Full text Cite

INVESTIGATION OF EXPERIMENTAL PARAMETERS FOR SURFACE-ENHANCED RAMAN SCATTERING (SERS) USING SILVER-COATED MICROSPHERE SUBSTRATES.

Journal Article Applied Spectroscopy · January 1, 1987 The effects of experimental parameters on surface-enhanced Raman scattering (SERS) for silver-coated microsphere substrates were investigated. The parameters included sphere size, silver thickness, and excitation wavelength. The SERS signal intensity varie ... Full text Cite

ENHANCED ROOM-TEMPERATURE PHOSPHORESCENCE OF ANTHRACENE ON CYCLODEXTRIN-TREATED FILTER PAPER.

Journal Article Applied Spectroscopy · 1987 This study investigates the enhancement of room-temperature phosphorescence (RTP) for anthracene when the compound is adsorbed on cyclodextrin (CD)-treated filter paper. The results show that beta -CD treatment induces a significant increase in anthracene ... Cite

Surface-enhanced Raman analysis of benzo[a]pyrene-DNA adducts on silver-coated cellulose substrates

Journal Article Appl. Spectrosc. (USA) · 1987 Benzo[a]pyrene-7,8-diol-9,10 epoxide (BPDE)-DNA adducts have been analyzed by surface-enhanced Raman scattering (SERS) spectrometry. BPDE is the ultimate carcinogenic metabolite of benzo[a]pyrene. The SERS measurements were performed with the use of a silv ... Cite

External Heavy-Atom Effect in Room-Temperature Phosphorescence

Journal Article Analytical Chemistry · January 1, 1987 The effect of external heavy-atom perturbers on the room-temperature phosphorescence (RTP) spectrum of dlbenzo-[f,h]quinoxallne (DBQ) adsorbed on Whatman 4 filter paper is reported. The totally symmetric vibrational bands are predominantly enhanced, leadin ... Full text Cite

Direct synchronous luminescence detection of co-eluting solutes in pseudophase liquid chromatography

Journal Article Journal of Chromatography A · January 1, 1987 Synchronous luminescence (SL) was used as the detection method for liquid chromatography (LC) to identify polynuclear aromatic (PNA) components that co-elute in the course of an LC separation. As many as five compounds could be identified from a single chr ... Full text Cite

Synchronous Fluorescence Measurement Of Bap Metabolites In Human And Animal Urine

Journal Article Analytical Letters · January 1, 1987 Injection of Benzo(a)pyrene into rats results in urinary excretion of fluorescent metabolites of BaP. The synchronous fluorescence (SF) technique can detect picogram quantities of fluorescent compounds such as 7, 8, 9, 10-tetrahydro-tetrahydroxy-benzo(a)py ... Full text Cite

Synchronous Luminescence Screening for Polynuclear Aromatic Compounds in Environmental Samples Collected at a Coal Gasification Process Develonment Unit

Journal Article American Industrial Hygiene Association Journal · July 1, 1986 An evaluation of the synchronous luminescence screening technique for rapid analysis of environmental samples is described. Synchronous luminescence is employed to estimate the content of polynuclear aromatic species of product gas particulate samples, and ... Full text Cite

PORTABLE FIBEROPTIC MONITOR FOR FLUORIMETRIC BIOASSAYS.

Journal Article Applied Spectroscopy · January 1, 1986 A simple and portable fiberoptic instrument for fluorimetric bioassays is described. The instrument is designed to be readily adaptable to commercially available biotesting wells on microplates. The microwells can also be used as interchangeable sensor hea ... Full text Cite

Sensitized Fluorescence Spectrometry Using Solid Organic Substrate

Journal Article Analytical Chemistry · January 1, 1986 This study investigated a simple technique based on sensitized luminescence for detecting trace amounts of polynuclear aromatic (PNA) compounds. Anthracene was used as the sensitizer designed to absorb excitation energy and funnel it to the guest analyte c ... Full text Cite

Surface-enhanced Raman spectrometry with silver particles on stochastic-post substrates

Journal Article Analytica Chimica Acta · January 1, 1986 A special type of substrate for surface-enhanced Raman scattering (s.e.r.s.) is evaluated. The substrates consist of silver particles deposited on stochastically arranged SiO2 posts produced by plasma etching of a quartz surface using a silver island film ... Full text Cite

Detection of Nitro Polynuclear Aromatic Compounds by Surface-Enhanced Raman Spectrometry

Journal Article Analytical Chemistry · January 1, 1986 The various nitro polynuclear aromatic compounds, Including 1-nitropyrene, 9-nitroanthracene 2-nitronaphthalene, and 2-nitrofluorene have been investigated by use of the surface-enhanced Raman scattering (SERS) spectrometry technique. Silver-coated substra ... Full text Cite

Hydrogen-bonding properties of a room-temperature phosphorescence cellulose substrate

Journal Article Journal of Physical Chemistry · January 1, 1986 The luminescence of heterocyclic compounds on Whatman 4 filter paper, a substrate frequently used in room-temperature phosphorimetry, is investigated with the view to characterizing the molecule-substrate interaction. Three molecules having known spectrosc ... Full text Cite

Fluorescence Line-Narrowing Spectrometry of Polycyclic Compounds on Filter Paper Substrates

Journal Article Analytical Chemistry · January 1, 1986 The fluorescence line-narrowing (FLN) technique was used to characterize polynuclear aromatic compounds adsorbed on a simple and practical substrate consisting of a filter paper material. Narrow-based excitation was used to produce highly resolved fluoresc ... Full text Cite

Development of a Dosimeter for Personnel Exposure to Vapors of Polyaromatic Pollutants

Journal Article Environmental Science and Technology · October 1, 1985 A new personnel dosimeter based on molecular diffusion and direct detection by room temperature phosphorescence (RTP) has been developed to monitor vapors of polynuclear aromatic (PNA) pollutants. The dosimeter is a simple, pen-size device that requires no ... Full text Cite

Detection of Specific Nitrogen-Containing Compounds by Room-Temperature Phosphorescence

Journal Article Analytical Chemistry · May 1, 1985 The use of room-temperature phosphorescence (RTP) for the selective analysis of nitrogen-containing heterocyclics has been investigated. The RTP emissions of azaarenes are enhanced in the presence of mercury(II) chloride, while those of homocyclic polyarom ... Full text Cite

Analysis of pseudouridine by fluorescence spectrometry

Journal Article Analytical Letters · January 1, 1985 Fluorescence spectral properties of pseudouridine, an important biological indicator, have been measured for the first time in this study. The fluorescence quantum yield is maximal in aqueous solution with pH values above 9 with spectral properties charact ... Full text Cite

Phosphorescence line narrowing of coronene and phenanthrene adsorbed on cellulose substrates

Journal Article Journal of Physical Chemistry · January 1, 1985 Phosphorescence line narrowing (PLN) of phenanthrene and coronene adsorbed on cellulose (filter paper) substrates was observed for the first time. The 457.9- and 514.5-nm lines of an argon ion laser were used to directly excite into the T1 ← S0 transitions ... Full text Cite

Luminescence determination of benzoquinoline isomers in complex samples

Journal Article Analytica Chimica Acta · January 1, 1985 The room-temperature phosphorescence (r.t.p.) and fluorescence spectra of benzoquinoline isomers are investigated. The isomers can be resolved into the linear or angular subgroups on the basis of their fluorescence and r.t.p. spectra by using conventional ... Full text Cite

Analysis of pseudouridine by fluorescence spectrometry

Journal Article Analytical Letters · 1985 Cite

Silver particles on stochastic quartz substrates providing tenfold increase in Raman enhancement

Journal Article Journal of Physical Chemistry · January 1, 1985 Exceptionally strong Raman enhancements have been observed for molecules adsorbed on silver deposited onto etched quartz substrates. A stochastic arrangement of tall quartz posts, with an average spacing of ≃110 nm, is obtained by plasma etching of SiO2 us ... Full text Cite

EFFECTIVENESS AND COST BENEFIT OF A LUMINESCENCE SCREENING PROCEDURE FOR POLYAROMATIC POLLUTANTS IN AMBIENT AIR.

Journal Article Proceedings, Annual Meeting - Air Pollution Control Association · December 1, 1984 This paper describes the use of a cost-effective screening procedure based on two simple luminescence techniques, synchronous luminescence (SL) and room temperature phosphorescence (RTP). The techniques are designed to rank air samples according to their t ... Cite

AIR POLLUTION: APPLICATIONS OF SIMPLE LUMINESCENCE TECHNIQUES.

Journal Article Identif and Anal of Org Pollut in Air · December 1, 1984 This paper provides an overview of the principles and applications of two simple spectroscopic methods synchronous luminescence (SL) and room temperature phosphorescence (RTP). These methods offer a variety of analytical applications and provide two extrem ... Cite

Luminescence of 1,4-naphthoquinone and the vitamin K system in Shpolskii matrices at 4K

Journal Article Spectrochim. Acta A, Mol. Spectrosc. (UK) · 1984 Investigates the high-resolution phosphorescence spectra of 1,4-naphthoquinone and the vitamin K system in Shpolskii solvents at 4K. The quasi-linear vibronic bands are discussed with regard to spectral assignments and polarization data. The effect of non- ... Full text Link to item Cite

Surface-Enhanced Raman Spectrometry for Trace Organic Analysis

Journal Article Analytical Chemistry · January 1, 1984 The analytical potential of a new spectrochemlcal method based on surface-enhanced Raman spectrometry (SERS) on silver-spheres-coated substrates Is presented for the first time In this work. A new method for preparing SERS-active substrate using submicron ... Full text Cite

A ranking index to characterize polynuclear aromatic pollutants in environmental samples

Journal Article Environment International · January 1, 1984 This study investigated the use of a screening procedure to provide a ranking index (RI) for characterizing polynuclear aromatic (PNA) species in atmospheric samples. The ranking procedure employs a cost-effective and rapid screening technique based on syn ... Full text Cite

Luminescence of 1,4-naphthoquinone and the vitamin K system in Shpolskii matrices at 4 K

Journal Article Spectrochimica Acta Part A: Molecular Spectroscopy · 1984 This work investigates the high-resolution phosphorescence spectra of 1,4-naphthoquinone and the vitamin K system in Shpolskii solvents at 4 K. The quasi-linear vibronic bands are discussed with regard to spectral assignments and polarization data. The eff ... Cite

Monitoring Exposure to Polynuclear Aromatic Compounds via Room Temperature Phosphorescence from Solid Substrates

Journal Article Radiation Protection Dosimetry · December 1, 1983 Room Temperature Phosphorescence (RTP) Spectroscopy is a relatively new solid state luminescence technique for monitoring polynuclear aromatic (PNA) compounds. The RTP technique can detect a variety of PNA pollutants at subnanogram levels. Practical applic ... Full text Cite

NEW INSTRUMENTS FOR PLANT AREA AND PERSONAL MONITORING.

Journal Article Alternative Energy Sources: Proceedings of the Miami International Conference on Alternative Energ · December 1, 1983 Cite

Screening air samples for polynuclear aromatic compounds: a field study.

Journal Article · December 1, 1983 The use of simple and cost-effective luminescence techniques for screening air-particulate samples is described. Two analytical methods, synchronous luminescence and room-temperature phosphorescence, are employed to monitor the content of polynuclear aroma ... Cite

Screening air samples for polynuclear aromatic compounds: a field study.

Journal Article · January 1, 1983 The use of simple and cost-effective luminescence techniques for screening air-particulate samples is described. Two analytical methods, synchronous luminescence and room-temperature phosphorescence, are employed to monitor the content of polynuclear aroma ... Cite

Surface detection of contamination: Principles, applications and recent developments

Journal Article Journal of Environmental Sciences · January 1, 1983 This paper presents the basic principles of luminescence spectroscopy used to detect trace organic substances on surfaces and some examples of application related to surface luminescence detection. These applications include the analysis of a synthetic fue ... Cite

RAPID SCREENING LUMINESCENCE TECHNIQUES FOR TRACE ORGANIC ANALYSIS.

Journal Article ASTM Special Technical Publication · January 1, 1983 This paper provides an overview of the principles and applications of two spectroscopic methods, synchronous luminescence (SL) and room-temperature phosphorescence (RTP), used as rapid screening tools for monitoring polynuclear aromatic compounds in real-l ... Full text Cite

SYNCHRONOUS LUMINESCENCE SPECTROSCOPY: METHODOLOGY AND APPLICABILITY.

Journal Article Applied Spectroscopy · January 1, 1982 A discussion is presented of the figures of merit associated with the synchronous luminescence (SL) technique in multicomponent analysis of complex samples. Spectral interferences and experimental factors that influence the choice of experimental condition ... Full text Cite

Direct determination of selected polynuclear aromatic hydrocarbons in a coal liquefaction product by synchronous luminescence techniques

Journal Article Analytica Chimica Acta · April 1, 1981 Synchronous fluorescence and the room-temperature phosphorescence methods were used to determine selected polynuclar aromatic hydrocarbons in a coal liquid (solvent-refined coal) product without a preseparation step. The procedure identified and quantified ... Full text Cite

The lightpipe luminoscope for monitoring occupational skin contamination.

Journal Article American Industrial Hygiene Association journal · February 1981 A portable luminescence detector has been constructed for monitoring occupational skin contamination. The instrument design is based upon a fiberoptics bifurcated lightguide which transmits the ultraviolet excitation light to the skin area being monitored ... Full text Cite

New luminescence techniques simplify air analysis

Journal Article InTech · January 1, 1981 Luminescence methods have been developed for air pollution measurement applications. Synchronous excitation and room temperature phosphorescence techniques are appropriate for analyzing atmospheric pollutants extracted from high volume samplers as well as ... Cite

RECENT DEVELOPMENT OF SIMPLE LUMINESCENCE TECHNIQUES FOR THE ANALYSIS OF AIR SAMPLES.

Journal Article Analysis Instrumentation: Proceedings of The Annual ISA Analysis Instrumentation Symposium · January 1, 1981 This paper reports the recent developments in luminescence analysis of atmospheric samples by using two simple luminescence methods, the synchronous excitation and the room temperature phosphorescence (RTP) techniques. Samples extracted from high-volume sa ... Cite

LUMINESCENCE MONITORING OF OIL OR TAR CONTAMINATION FOR INDUSTRIAL HYGIENE.

Journal Article Nuclear instruments and methods · January 1, 1980 Synfuel plants produce potentially carcinogenic oils and tars. Exposure of workers to these tars and oils is difficult to avoid completely and occurs via direct contact with dirty surfaces or condensation of escaped fumes onto or within the body. Surface s ... Full text Cite

Identification and quantification of polynuclear aromatic compounds in synthoil by room-temperature phosphorimetry

Journal Article Analytica Chimica Acta · January 1, 1980 Room-temperature phosphorimetry (r.t.p.) is used to identify and quantify polynuclear aromatic compounds in a synthetic fuel (Synthoil). Several trace and major components, benzo[a]pyrene, chrysene, fluorenes, fluoranthene, phenanthrene, and pyrene, have b ... Full text Cite

The applicability of the second-derivative method to room-temperature phosphorescence analysis

Journal Article Analytica Chimica Acta · January 6, 1979 The use of the second-derivative (d2) technique to overcome some of the problems encountered in room-temperature phosphorescence analysis is investigated. Despite some reduction in the signal-to-noise values, the d2-technique was shown to be particularly s ... Full text Cite

Selective Heavy-Atom Perturbation for Analysis of Complex Mixtures by Room-Temperature Phosphorimetry

Journal Article Analytical Chemistry · January 1, 1979 The technique of selective external heavy-atom pertubation (SEHAP) is applied to improve room temperature phosphorescence (RTP) analysis of complex samples. It is demonstrated that heavy atoms can selectively increase the phosphorescence emission of specif ... Full text Cite

Multicomponent analysis by synchronous luminescence spectrometry.

Journal Article Analytical chemistry · March 1978 Full text Cite

Synchronous Spectroscopy for Analysis of Polynuclear Aromatic Compounds

Journal Article Environmental Science and Technology · January 1, 1978 The technique of synchronous excitation is used to enhance the selectivity of luminescence spectrometry. The applicability of this simple methodology to the monitoring of trace organic pollutants such as the polynuclear aromatic compounds (PNA) originating ... Full text Cite

Singlet-Triplet Energy Difference as a Parameter of Selectivity in Synchronous Phosphorimetry

Journal Article Analytical Chemistry · January 1, 1978 The direct use of the singlet-triplet energy difference as a new factor of selectivity in phosphorimetry is suggested. The methodology, which is based on the synchronous excitation technique, exploits the specificity of energy gaps ΔST between the phosphor ... Full text Cite

RAPID ANALYSIS OF PNA COMPOUNDS IN COMPLEX SAMPLES BY ROOM TEMPERATURE PHOSPHORIMETRY.

Journal Article Preprints - Tribology Conference · January 1, 1978 Described is the methodology of the Room Temperature Phosphorimetry (RTP), a relatively new technique based on the phosphorescence at room temperature of organic compounds adsorbed on various solid substrates including asbestos, silica gel, sodium acetate, ... Cite

Room-temperature phosphorescence of several polyaromatic hydrocarbons.

Journal Article Talanta · February 1977 The room-temperature phosphorescence of several polyaromatic hydrocarbons (anthracene, coronene, phenanthrene, pyrene, benzo[a]pyrene) and carbazole on paper support has been investigated. Heavy-atom solvents such as silver nitrate and sodium iodide were f ... Full text Cite

Room temperature phosphorimetry as a new spectrochemical method of analysis

Journal Article Appl. Spectrosc. Rev. (USA) · 1977 A review of the recent advances in methodology and instrumentation applied to room temperature phosphorimetry is presented. The general principles of the method are first discussed, followed by methodology and instrumentation. Characteristic features of ro ... Cite

Comparison of multichannel SIT image vidicon and photomultiplier sequential linear scanning systems for the measurement of steady-state and transient fluorescence of molecules in solution

Journal Article Anal. Chem. (USA) · 1977 The feasibility of using the SIT image vidicon as a potential detector for liquid chromatography is demonstrated. Limits of detection range from ~2×10-2 ng for anthracene to ~6×10-1 ng for riboflavin. A direct experimental comparison is made of ... Cite

Instrument for the facilitation of room temperature phosphorimetry with a continuous filter paper device

Journal Article Anal. Chem. (USA) · 1977 The design of a simple and unique automated phosphorimetric instrument is described. The apparatus, which was developed for a new method of analysis based on room temperature phosphorescence of adsorbed organic molecules on filter paper, uses a simple rota ... Cite

A SIT image detector in analytical fluorescence spectrometry

Journal Article Spectrochim. Acta A, Mol. Spectrosc. (UK) · 1977 The use of a silicon intensified target vidicon optical multichannel analyser system to replace the photomultiplier detector for a spectrophotofluorometer is described. The performance of this system is examined ... Full text Link to item Cite

A SIT image detector in analytical fluorescence spectrometry

Journal Article Spectrochimica Acta Part A: Molecular Spectroscopy · 1977 Cite

Fluorescence studies of benzo-[a]-pyrene in liposome membrane systems.

Journal Article Biochemical and biophysical research communications · November 1976 Full text Cite

Fluorescence from the second excited singlet state of [18] annulenes

Journal Article Chemical Physics Letters · August 1, 1976 The emission spectra of the second excited singlet state of [18] annulene and of monofluoro [18] annulene (in a 3-methylpentane glass at 4 K) are reported. The large energy gap between the first and second excited singlet states inhibits fast internal conv ... Full text Cite

Heavy-atom effect on room temperature phosphorimetry.

Journal Article Analytical chemistry · July 1976 Full text Cite

Quasilinear fluorescence of pyrene in a monocrystalline matrix of n-heptane

Journal Article Chemical Physics Letters · April 1, 1976 The fluorescence spectrum of pyrene oriented in a pseudo-monocrystalline matrix of n-heptane has been studied at 8 K. Quasilinear and polarized emission emanates predominantly from a single site having its 0-0 transition at 26926 cm-1. A vibrational analys ... Full text Cite

Phosphorescence spectra from selected sites of N-ethylcarbazole in n-alkanes

Journal Article Chemical Physics Letters · February 1, 1974 The phosphorescence spectra of N-ethylcarbazole in n-alkanes consist of sharp lines which may be grouped in slightly shifted subspectra. For n-heptane, using narrow band excitation, distinct subspectra were observed at 4°K. Each subspectrum is associated w ... Full text Cite

HIGH RESOLUTION LUMINESCENCE SPECTROMETER EM DASH 2. DATA TREATMENT AND CORRECTED SPECTRA.

Journal Article Applied Optics · 1974 Computer processing of the digital output of a high resolution luminescence spectrometer is discussed. The data treatment is based upon statistical concepts. Subroutines for wavelength calibration, dark count correction, data smoothing, and curve presentat ... Cite

High resolution luminescence spectrometer. I: simultaneous recording of total luminescence and phosphorescence

Journal Article Applied Optics · 1973 A luminescence spectrometer is described that has the particular advantage of allowing the simultaneous recording of total luminescence and phosphorescence spectra. The basic design is straightforward and uses commercially available components. The measuri ... Cite

Laser excited luminescence of coronene in a shpolskii matrix

Journal Article Journal of Luminescence · January 1, 1973 The luminescence spectrum of coronene in n-heptane at 77°K has been studied using both xenon lamp and argon ion laser excitation. The results illustrate the Shpolskii theory of different emitting sites. With xenon excitation the emission spectrum consists ... Full text Cite

Phosphorescence spectra and lifetimes of 1,4-naphthoquinone, vitamin K3 and K1

Journal Article Z. Phys. Chem., Leipz. (East Germany) · 1972 The luminescence spectra of 1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), and 2-methyl-3-phytyl-1,4-naphthoquinone (vitamin K1) were studied at 77 K in a hydrocarbon glass. In all three cases the nature of the luminescence can be assigned t ... Cite